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Offlinenoppie
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Panaeolus cyanescens pftek jars looks dried
    #23497660 - 08/01/16 06:03 AM (7 years, 5 months ago)

I have 4 pan cyan pftek jars (cow manure/brf/verm mix)

13 days passed since inoculated no any colonization signs
I guess jars inside is dried
Why no any colonization sign after 13 days?

Should I do some injection distilled water inside jars?


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InvisiblebodhisattaMDiscordReddit
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Re: Panaeolus cyanescens pftek jars looks dried [Re: noppie]
    #23497712 - 08/01/16 07:03 AM (7 years, 5 months ago)

Should start spores of species other than cubensis out on agar.

And post pictures

And never inject a failed jar, just start over


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Offlinenoppie
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Re: Panaeolus cyanescens pftek jars looks dried [Re: bodhisatta]
    #23497776 - 08/01/16 07:39 AM (7 years, 5 months ago)

Quote:

Trusted Cultivator said:
Should start spores of species other than cubensis out on agar.

And post pictures

And never inject a failed jar, just start over




I have spore-prints righnow, how should i start spores on agar? Could you please give me link for step by step instructions for spore-print to agar?


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InvisiblebodhisattaMDiscordReddit
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Re: Panaeolus cyanescens pftek jars looks dried [Re: noppie]
    #23497830 - 08/01/16 08:18 AM (7 years, 5 months ago)

Already did. Fourth link down in my signature(says agar). And

https://www.shroomery.org/forums/dosearch.php?where=body&tosearch=main&how=all&words=Agar&newerval=5&newertype=y&limit=25&showmain=1&expert=1&image=1&sort=r&way=d

And

The first two links also have recommended threads


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Offlinenoppie
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Re: Panaeolus cyanescens pftek jars looks dried [Re: bodhisatta]
    #23497921 - 08/01/16 09:23 AM (7 years, 5 months ago)

Thank you

Also please see
Here's the photo of 13th day with no colonization sign




Quote:

Trusted Cultivator said:
Already did. Fourth link down in my signature(says agar). And

https://www.shroomery.org/forums/dosearch.php?where=body&tosearch=main&how=all&words=Agar&newerval=5&newertype=y&limit=25&showmain=1&expert=1&image=1&sort=r&way=d

And

The first two links also have recommended threads




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InvisiblebodhisattaMDiscordReddit
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Re: Panaeolus cyanescens pftek jars looks dried [Re: noppie]
    #23497928 - 08/01/16 09:28 AM (7 years, 5 months ago)

Using pint jars and foil lids is last ditch, I woke up in rural Africa and need to trip, technology.

That's probably half your problem right there.

I would get some jars and make appropriate lids


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Offlinenoppie
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Re: Panaeolus cyanescens pftek jars looks dried [Re: bodhisatta]
    #23497953 - 08/01/16 09:45 AM (7 years, 5 months ago)

Quote:

Trusted Cultivator said:
Using pint jars and foil lids is last ditch, I woke up in rural Africa and need to trip, technology.

That's probably half your problem right there.

I would get some jars and make appropriate lids





This is waterglass not pint jar, i do colonize copelandia a few times with no problem.
But this mixture of cow manure/brf/verm with pan cyan is my first attempt. (Print to sryinge then inoculate into jar)

Is that method right? Should i wait ? How many days?


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Offlinenoppie
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Re: Panaeolus cyanescens pftek jars looks dried [Re: noppie]
    #23506226 - 08/03/16 03:49 PM (7 years, 5 months ago)

Today 16th day since inoculated, no any difference :frown:(((



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InvisibleInocuole
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Re: Panaeolus cyanescens pftek jars looks dried [Re: noppie]
    #23506266 - 08/03/16 04:04 PM (7 years, 5 months ago)

That's the same exact picture.


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Re: Panaeolus cyanescens pftek jars looks dried [Re: Inocuole]
    #23506725 - 08/03/16 06:03 PM (7 years, 5 months ago)

:lmafo:


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Offlinenoppie
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Re: Panaeolus cyanescens pftek jars looks dried [Re: Psilosoulful]
    #23508952 - 08/04/16 09:57 AM (7 years, 5 months ago)

Here's 17th day of my manure jars :frown:



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InvisibleMad Season
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Re: Panaeolus cyanescens pftek jars looks dried [Re: noppie]
    #23508963 - 08/04/16 10:00 AM (7 years, 5 months ago)

I see a tiny spot growing on the last 3 o.o. Why sad?


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Offlinenoppie
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Re: Panaeolus cyanescens pftek jars looks dried [Re: Mad Season]
    #23509067 - 08/04/16 10:33 AM (7 years, 5 months ago)

Quote:

Mad Season said:
I see a tiny spot growing on the last 3 o.o. Why sad?




I am worrying 17th day since inoculated any ony see that tiny spot which i am not sure it's mycelium because it's same dimensions 4 days ago :frown:

Nothing grows on those jars :frown: why? :frown:(4


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Re: Panaeolus cyanescens pftek jars looks dried [Re: noppie]
    #23509074 - 08/04/16 10:36 AM (7 years, 5 months ago)

Why are you doing pan cyan as pf tek anyway?  That's not even something I'd feel confident enough to pull off.  I'm sure they'd feel much more at home in a substrate that can retain moisture properly.


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Re: Panaeolus cyanescens pftek jars looks dried [Re: Inocuole]
    #23509082 - 08/04/16 10:40 AM (7 years, 5 months ago)

Quote:

Inocuole said:
Why are you doing pan cyan as pf tek anyway?  That's not even something I'd feel confident enough to pull off.  I'm sure they'd feel much more at home in a substrate that can retain moisture properly.




I am totally newbie :smile: pftek is easy. Which tek do you prefer for pan cyan?


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Re: Panaeolus cyanescens pftek jars looks dried [Re: noppie]
    #23509122 - 08/04/16 10:53 AM (7 years, 5 months ago)

More like, which species do I prefer for PF tek, to which the answer is cubensis.  For cyans some kind of cased bulk substrate would really be better.


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Offlineleech911
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Re: Panaeolus cyanescens pftek jars looks dried [Re: Inocuole]
    #23509140 - 08/04/16 11:01 AM (7 years, 5 months ago)

Pan Cyanes are also a bit trickier for a newer grower. If I were you, I'd start with cubes and stick to the tek.


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Re: Panaeolus cyanescens pftek jars looks dried [Re: Inocuole]
    #23510021 - 08/04/16 03:58 PM (7 years, 5 months ago)

Quote:

Inocuole said:
More like, which species do I prefer for PF tek, to which the answer is cubensis.  For cyans some kind of cased bulk substrate would really be better.




For colonization period what kind of bulk substrate do you prefer?


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InvisibleInocuole
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Re: Panaeolus cyanescens pftek jars looks dried [Re: noppie]
    #23510025 - 08/04/16 03:59 PM (7 years, 5 months ago)

Um... I don't understand the question.  For colonization period?  I don't use a bulk substrate for colonization.


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Offlinenoppie
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Re: Panaeolus cyanescens pftek jars looks dried [Re: leech911]
    #23510028 - 08/04/16 04:00 PM (7 years, 5 months ago)

Quote:

leech911 said:
Pan Cyanes are also a bit trickier for a newer grower. If I were you, I'd start with cubes and stick to the tek.




I do cubensis several times with pftek now i need an easy tek for colonization isn't that pftek safe and easy for also pan cyanes?


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