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weetsie
unlicensed tub surgeon



Registered: 05/08/11
Posts: 572
Loc: United Kingdom
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Re: Panaeolus cambodginiensis var. Sandose Trays ready to be cased [Re: ravenfeeder]
#23519399 - 08/07/16 04:17 PM (7 years, 5 months ago) |
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Good luck man, I'm casing my cambos tomorrow. 
Will be watching how you get on
-------------------- Active grow logs: Oysters on Straw Pellets Trade list
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ravenfeeder
Wherever I May Grow



Registered: 01/29/13 
Posts: 238
Loc: South Africa
Last seen: 1 year, 3 months
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Re: Panaeolus cambodginiensis var. Sandose Trays ready to be cased [Re: weetsie]
#23542924 - 08/15/16 04:45 AM (7 years, 5 months ago) |
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Quote:
weetsie said: Good luck man, I'm casing my cambos tomorrow. 
Will be watching how you get on 
Hi All,
In the meantime I have been patching the whole surface as overlay was taking place (kinda to be expected?) around day 8, and still nothing has transpired... I've even the scratched the whole casing, on day 12, as I feared it might have been too thick, especially after patching, and also hydrated the substrate with some water, as the tray did seem to feel less weighty...
My suspicion is that the hydrated lime I've added to the substrate, to balance the pH to just above 7, might have affected the non-pin formation, and perhaps what looked like Mycogone perniciosa before casing, might also have influenced this?
On the other hand, I've invested in some filter patched spawn bags, an impulse sealer, and straw (mainly for Oysters), and have now prepared a Panaeolus substrate of note; One that most TC's here have used to great success, and I'll be sterilizing it this time, just like StoneSun has done with great results, as I'm kinda fed up of trying pasteurization and trays with these delicate Genus & species. I've customized the recipe a tad, but it should work well, especially seeing as I added no hydrated lime this time, and (surprisingly) the pH is around 6.8 - 7.0 
The substrate now comprises of:
20 cups/5 L/50% Field-Aged Horse Manure 8 cups/2 L/20% Coco Coir 6 cups/1.5 L/15% Chopped Straw (shorter than 1") 4 cups/1 L/10% Vermiculite 2 cups/500 ml/5% Gypsum
10 cups/2.50 L/25% Water (up to field capacity).
After about 12 hours the substrate's field capacity seems just right (leaning slightly towards the dry side, which I've read are the way Pans like it), and I've filled each 250 mm x 330 mm spawn bag with 3.5 cups/875 ml of prepared substrate, shaped it on a flat surface and measured the substrate height level at about 1.5". Thereafter I rolled the bag with the filter patch's exterior side pointing inwards, until filter patch's interior side nearly fully faces upwards, and tape it firmly to keep it firmly rolled up. Conveniently, the opening ends of each bag ended up on the side of the rolled-up bag, pointing downwards, thus there should be no way that steam/water during sterilization can get in the bag - This way I won't have to use excessive amounts of aluminum foil to cover bags each time I PC some.
Then I stacked the bags in sterilizer, leaving spaces in between the bags as to allow the steam to cover most of the bags' surfaces effectively - I also won't need to use a bunch of ring lids as spacers in between the bags and the PC's side wall, as they all fit inside so perfectly, as you can see below:

Once sterilized for 2 hours at 15 psi~250 degrees Fahrenheit and cooled down, I'll open the PC in front of my HEPA flow hood (designed and built according to StoneSun's tek - I salute you sir! ), Open up the bags - that tend to almost vacuum-seal itself when the PC depressurizes - and knock it up with about 1.5 cups of good-looking Pan cam Millet-, Rye- and Oat-spawn, which should bring the substrate height to between 1.5 to 2 inches, and impulse seal twice or thrice (extra sure it's sealed!), shake it up good, shape the substrate again at the base of the bag, and allow to colonize at 72 to 80 degrees Fahrenheit in ambient, indirect fruiting light.
I'll keep you posted!
 
-------------------- "Live & Let Live"
     
Edited by ravenfeeder (08/15/16 06:48 AM)
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Leftfield420
bong toker



Registered: 02/26/16
Posts: 10,023
Last seen: 7 hours, 45 minutes
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Re: Panaeolus cambodginiensis var. Sandose Trays ready to be cased [Re: PsilocyBen17]
#23551865 - 08/18/16 12:03 AM (7 years, 5 months ago) |
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Quote:
PsilocyBen17 said:
Quote:
r35l said: i...5th grade science guys..5th grade. that person giving you bunk infos
Hahaha this guy

I'm pretty sure I looked like this after reading that
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ravenfeeder
Wherever I May Grow



Registered: 01/29/13 
Posts: 238
Loc: South Africa
Last seen: 1 year, 3 months
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Re: Panaeolus cambodginiensis var. Sandose Trays ready to be cased [Re: Leftfield420]
#23564919 - 08/22/16 02:32 AM (7 years, 5 months ago) |
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Hi All!
I've tossed out the trays, and in the meantime the new Panaeolus cambodginiensis var. Sandose Domesticate bags have been colonizing fairly well. Thus far only 2 out of the 16 bags have contaminated on Day 2, and been tossed out asap (source = same spawn jar), and it is currently Day 6, and the bags that were spawned from millet and oats are actually all fully colonized! Only the bags spawned from rye seems to be lagging behind, thus I'll only be using oats and millet as my Pan cam spawn henceforth...
Here are pictures of the bags taken this morning, where they colonize in ambient indirect 6500k CFL lighting at mostly a temperature range of 72 - 81 degrees Fahrenheit:

Now I'll wait 3 to 6 days to allow the mycelium to properly consolidate the substrate - and REALLY become amped to fruit - and cut it open down to the height of the substrate's surface, case it with approximately a ¼" thin layer of field capacity modified 50/50+ pasteurized casing material. (This time I'll add a bit more vermiculite than peat moss, and also a bit of perlite, as I've seen highly successful grows with this added by Stonesun and BlindingLeaf Up'ing aeration at the substrate level can only be a good thing!). The pH will be adjusted with hydrated lime to between 7 and 8.
Love & Light!
   
-------------------- "Live & Let Live"
     
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Panaeolus cambodginiensis var. Sandose Trays ready to be cased [Re: ravenfeeder]
#23565136 - 08/22/16 06:35 AM (7 years, 5 months ago) |
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thats a unique way to grow. I like the large sample size.
i use verm at 25%. I don't use hydrated lime. just limestone. I don't check PH, but its probably 7.
I'm definitely interested in seeing if consolidation helps with those god damn overlaying cambos, so I'm rooting for ya dude
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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ravenfeeder
Wherever I May Grow



Registered: 01/29/13 
Posts: 238
Loc: South Africa
Last seen: 1 year, 3 months
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Re: Panaeolus cambodginiensis var. Sandose Trays ready to be cased [Re: blindingleaf]
#23589343 - 08/29/16 08:35 AM (7 years, 4 months ago) |
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Hi All!
It's just been over 7 Days, and here's the update on the bags, including - most importantly - the pictures 
I've allowed all the fully colonized Millet and Oats sterile substrate bags to consolidate for 6 days, and cased it very thinly, in front of my running flow hood (to ensure the cleanest casing application ) with the pasteurized casing material I'll also mention that the bags spawned with rye never colonized completely, yet became firmer, and so I decided to sacrifice it and practice my casing application skills on it and placed it outside to see how it will fair in an abundance of fresh air

You'll notice from the the top shots that some of the cased bags' surface's edges tend to be exposed as the casing drops off and down the side of the substrate and the bag - Will this affect the grow/pinset negatively, and should I simply patch the exposed edges later on? 
Thanks in Advance!
   
-------------------- "Live & Let Live"
     
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Panaeolus cambodginiensis var. Sandose Trays ready to be cased [Re: ravenfeeder]
#23589363 - 08/29/16 08:44 AM (7 years, 4 months ago) |
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i would play it by ear. those edges may even be where ur first pins emerge.
careful with doing the dirty in front of ur hood. not even a half hour ago I broke a jar of rye in front of the running hood and had to stop everything and vacuum 
luckily, grains dont "smudge" and are large enough for the vacuum to pick up (not to mention they are sterilized). peat, poo, coir, not as much. i see u got a nice screen though, so bumping by accident shouldn't be an issue.
im interested to see effect of consolidating. I'm going to try fruiting at spawning next. with two ends of the spectrum, maybe we can figure this out
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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PutACapInHisAss
Stranger Than Fiction



Registered: 08/11/07
Posts: 252
Loc: Earth
Last seen: 6 years, 4 months
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Re: Panaeolus cambodginiensis var. Sandose Trays ready to be cased [Re: blindingleaf]
#23638749 - 09/12/16 03:12 PM (7 years, 4 months ago) |
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Quote:
blindingleaf said: they basically almost overlaid the second casing too by the time it fruited.
Second casing? Do you normally case pans twice?
If so...
When? Once at spawning to substrate and again at fruiting?
My cubes seem to do very well with early casing but clearly this changes a lot of factors that may not Pan out
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d0urd3n
Just call me "D"

Registered: 09/15/10
Posts: 5,237
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Re: Panaeolus cambodginiensis var. Sandose Trays ready to be cased [Re: PutACapInHisAss]
#23638827 - 09/12/16 03:37 PM (7 years, 4 months ago) |
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Clearly I'm not BL but what he meant was that he cases once, it ate the casing, so he patched it/cased it again, and it practically ate all the patches.
Ideally you only want to case once but they are aggressive so it's tricky.
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Panaeolus cambodginiensis var. Sandose Trays ready to be cased [Re: d0urd3n]
#23640609 - 09/13/16 04:41 AM (7 years, 4 months ago) |
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first casing got overlay, on all 3trays. that sucks…i knew they wouldn't pin except maybe from the sides. i had nothing to lose really, so i scratched and cased again…luckily it worked.
here is one tray where u can see it almost ate the whole second casing too, the other 2 didn't eat it to this extent.
 
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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PutACapInHisAss
Stranger Than Fiction



Registered: 08/11/07
Posts: 252
Loc: Earth
Last seen: 6 years, 4 months
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Re: Panaeolus cambodginiensis var. Sandose Trays ready to be cased [Re: blindingleaf]
#23641666 - 09/13/16 01:38 PM (7 years, 4 months ago) |
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Thanks for the clarification dudes!
While this thread is resurrected, how is it harvesting Pans to keep the substrate intact?
Can you pull/twist them? Do you have to cut them out with scissors or something like an Exacto? Other?
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