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nicelee808
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Registered: 05/25/16
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Agar dishes 1
#23494479 - 07/31/16 03:33 AM (7 years, 5 months ago) |
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Hi all.
Once the agar dishes have cooled. Can they be stored in the fridge. If so how long for. Also once the mycelium has grown can they be stored in the fridge
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nicelee808
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Just to clarify. Once i have done the dishes without being inoculated can they be stored in the fridge. Or whats the best storage pre inoculation. The dishes i have inoculated. As far as im aware should be stored in a dark place. Once fully colonized can they be stored in the fridge. Or would leaving them where they are be better
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nicelee808
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Also how long should i wait before enoculating
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mindbentempire



Registered: 10/26/09
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Hey. From what I know, you should be good to keep colonised dishes in the fridge for a few months.
For long term storage, slants are better. Use the search engine for that.
You can inoculate your dishes as soon as they are no longer hot.
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nicelee808
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Thanks mindbentempire. What about un inoculated dishes. Can they be stored in the fridge
Edited by nicelee808 (07/31/16 04:08 AM)
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LocN9ne
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They can be stored anywhere you would like... Right up until they dry out...
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mrmazdarx9
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Re: Agar dishes [Re: LocN9ne]
#23494661 - 07/31/16 05:40 AM (7 years, 5 months ago) |
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Its a good idea to leave a couple uninnoculated dishes out for about a week to check for any contams prior to their use after the week and there's no sign of contams then store them in the fridge if you want but there no real need as long they aren't dried out they are fine to use
Edited by mrmazdarx9 (07/31/16 01:58 PM)
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nicelee808
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Thanks all. Hoefully should get my copelandias going
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PsilocyBen17
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Fridges are dirty places and I never recomend storing anything in there, except for maybe slant tubes, but i'd be sure to store them in a clean ziplock container before putting them in the fridge....
Letting plates sit for a week to check for contaminations before inoculation is a silly idea. If this was important we'd be doing it with jars too. 99% of contaminations come from cultivator and the only way around them is good sterile technique.
As for your plates, they are fine to sit at room temp. I do mine in big batches and use them as needed.
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mrmazdarx9
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Quote:
PsilocyBen17 said: Fridges are dirty places and I never recomend storing anything in there, except for maybe slant tubes, but i'd be sure to store them in a clean ziplock container before putting them in the fridge....
Letting plates sit for a week to check for contaminations before inoculation is a silly idea. If this was important we'd be doing it with jars too. 99% of contaminations come from cultivator and the only way around them is good sterile technique.
As for your plates, they are fine to sit at room temp. I do mine in big batches and use them as needed.
Well ive done it for piece of mind to check my pour technique was good and im not wasting any spores on plates ive contaminated before ive started. Im new to agar anything that can help ensure a good plate isnt "silly" in my eyes
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r35l
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Registered: 07/18/16
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Quote:
mrmazdarx9 said:
Quote:
PsilocyBen17 said: Fridges are dirty places and I never recomend storing anything in there, except for maybe slant tubes, but i'd be sure to store them in a clean ziplock container before putting them in the fridge....
Letting plates sit for a week to check for contaminations before inoculation is a silly idea. If this was important we'd be doing it with jars too. 99% of contaminations come from cultivator and the only way around them is good sterile technique.
As for your plates, they are fine to sit at room temp. I do mine in big batches and use them as needed.
Well ive done it for piece of mind to check my pour technique was good and im not wasting any spores on plates ive contaminated before ive started. Im new to agar anything that can help ensure a good plate isnt "silly" in my eyes
fridges indeed have mold (if you ever let anything get moldy in there-EVER)
but the bacteria tho. your mouth and HANDS are directly related to everything in ther.... if your going to do it wrapped it before you slap it in there.. i been doing plastic wrap each plate then in two ziplocs in a tub kept where i keep my jars.
youre not wwasting spores on agar unless you are just dumping assloads out on the plate. you go to agar to isolate if anything you are spreading them out as long as you use a minimal amount of solution and even swipe with a loupe
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mrmazdarx9
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Re: Agar dishes [Re: r35l]
#23497666 - 08/01/16 06:17 AM (7 years, 5 months ago) |
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Literally none of that relates to what I said and pasty and supalemonhaze told me its a good idea to leave a couple blanks to check my pour technique so I'll listen to what they said
Quote:
Pastywhyte said: You have a lot of satellites there. Need to work on your movements. Never reach over the media, be sure your box is wet, use less spores. The tiniest speck visible is like 10 million spores. Technically you only need 2.
Practice makes perfect. If you are pouring plates be sure to leave a few blanks to make sure your pour techniques are good. Later you can try a dry run with em to be sure your movements are good. Flame the tools, open the plates, touch the loop to the agar, do everything you normally would except put spores on the loop. If you can do that and have nothing show up your technique is solid.
Quote:
Supalemonhaze said: Yeah, don't refrigerate blank plates. You want contams to grow if you have them so you can throw it out. Pouring is quite easy, you should do fine if you move slowly enough. Try doing a pour and leave a bunch of plates unused to see if your technique is good enough. If after a week nothing pops up, do what pasty said. Blank transfers are great for seeing where the faults lie.
When pouring agar, try to stack them as high as you can, without them getting too close to the top of the SAB ofc. The stack will prevent the ones on the bottom from clouding up with condensation. Someone above suggested stacks of 3, you should do bigger stacks. I notice that my first 3 in a stack all have at least some condensation, the one on top having the most. The ones below the 3rd will be crystal clear.
goodluck with it, keep us posted. I like seeing folks pour their own agar, people tend to think it's a lot harder than it actually is.
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Edited by mrmazdarx9 (08/01/16 06:27 AM)
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bodhisatta 
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Registered: 04/30/13
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Re: Agar dishes [Re: r35l]
#23497721 - 08/01/16 07:09 AM (7 years, 5 months ago) |
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Quote:
r35l said:
Quote:
mrmazdarx9 said:
Quote:
PsilocyBen17 said: Fridges are dirty places and I never recomend storing anything in there, except for maybe slant tubes, but i'd be sure to store them in a clean ziplock container before putting them in the fridge....
Letting plates sit for a week to check for contaminations before inoculation is a silly idea. If this was important we'd be doing it with jars too. 99% of contaminations come from cultivator and the only way around them is good sterile technique.
As for your plates, they are fine to sit at room temp. I do mine in big batches and use them as needed.
Well ive done it for piece of mind to check my pour technique was good and im not wasting any spores on plates ive contaminated before ive started. Im new to agar anything that can help ensure a good plate isnt "silly" in my eyes
fridges indeed have mold (if you ever let anything get moldy in there-EVER)
but the bacteria tho. your mouth and HANDS are directly related to everything in ther.... if your going to do it wrapped it before you slap it in there.. i been doing plastic wrap each plate then in two ziplocs in a tub kept where i keep my jars.
youre not wwasting spores on agar unless you are just dumping assloads out on the plate. you go to agar to isolate if anything you are spreading them out as long as you use a minimal amount of solution and even swipe with a loupe
I couldn't find the pictures of the agar work you've done. Links?
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mupetmower
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Registered: 03/29/16
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i wanna see this so called loupe 
by the way, OP, i store my unused plates in the same place i store my growing plates. which is in a cabinet. i have heard they can be stored for even a month, and be fine to use. like others have said, as long as they havent dried out.
really, just about anywhere is fine.. i have only put a few in the fridge, and those were because they hit the edge of the plate, and i wanted to use them to make LI, and didnt want them to grow much more until then. only for about a week, though.
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PsilocyBen17
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Quote:
PsilocyBen17 said:
Letting plates sit for a week to check for contaminations before inoculation is a silly idea. If this was important we'd be doing it with jars too. 99% of contaminations come from cultivator and the only way around them is good sterile technique.
I said this before I knewyou were using a pour tek....most new guys start with pasty plates....
even still, I wouldn't bother doing a contam check every time I pour new plates. I'd check once or twice until I knew my technique was solid, then just transfer as soon as they are cooled.
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mrmazdarx9
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Ah right I understand yeah im not very good yet to trust my technique so if I see ive poured good clean plates then I know ive got that part down well then if it goes bad in the inoculation then I know thats where my problem lies
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PsilocyBen17
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Yeah, but dont forget the whole point of agar is to develop clean growth by transfering away from contaminations. Its typical to see a bit of mold or bacteria in the first set of transfers, especially when cloning from a wild specimen.
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mrmazdarx9
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Yeah I understand that but if you had seen my first attempt youd get why I wanted to make sure my pour was good the thing looked like it could have got up and walked away
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