Home | Community | Message Board


Gaiana.nl
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Contamination Forum

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars, Rye Grain, Wild Bird Seed, pH Test Strips

Jump to first unread post. Pages: 1
Offlineragadinks
MrBeatle
 User Gallery

Registered: 10/20/03
Posts: 1,297
Last seen: 2 years, 8 months
Contam on WBS ( pics )
    #2349216 - 02/18/04 10:21 AM (12 years, 9 months ago)

I have just soaked WBS in diluted coffee and PC'd for about an hour. Both of the jars got a contam that is a kind of yellow sludge around the seeds. Does anybody know what that could be ?

Here is the jar:



And here are two close ups of the contaminations:





Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Re: Contam on WBS ( pics ) [Re: ragadinks]
    #2349232 - 02/18/04 10:25 AM (12 years, 9 months ago)

that looks like bacteria to me


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleJoshua
Holoman
Male

Registered: 10/27/98
Posts: 5,389
Loc: The Matrix
Re: Contam on WBS ( pics ) [Re: ragadinks]
    #2349325 - 02/18/04 11:06 AM (12 years, 9 months ago)

It is bacteria. You need a much better mix. You want something w/o sunflower seeds. Rye grain can also harbor high levels of endospores, which cannot be sterilized without first soaking for 12-24 hours.

Joshua


--------------------
The Shroomery Bookstore

Great books for inquiring minds!

"Life After Death is Saprophytic!"


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineragadinks
MrBeatle
 User Gallery

Registered: 10/20/03
Posts: 1,297
Last seen: 2 years, 8 months
Re: Contam on WBS ( pics ) [Re: Joshua]
    #2353662 - 02/19/04 06:39 AM (12 years, 9 months ago)

I guess I did not PC them long enough, because other jars with the same mix of WBS were all right. How long do you PC in general ? Is there a recomondation for the different substrates like WBW, corn, rye, millet etc. ?


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMAIA
World-BridgerKartikeya (DftS)
Male User Gallery

Registered: 04/27/01
Posts: 7,275
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 6 months, 11 days
Re: Contam on WBS ( pics ) [Re: ragadinks]
    #2355020 - 02/19/04 04:04 PM (12 years, 9 months ago)

Well, actually the substrate looks a bit dry and that's also a cause for contaminants to germinate.
I find it better if i precook the grain first, because it's easier to keep track of the water content. When you reach an optimal absorption (before the kernels start bursting), drain the water, fill the jars with the kernels and PC them. The timings can vary but it should be at least 45 min. IMHO. I normally do 60 min.

MAIA


--------------------
Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineragadinks
MrBeatle
 User Gallery

Registered: 10/20/03
Posts: 1,297
Last seen: 2 years, 8 months
Re: Contam on WBS ( pics ) [Re: MAIA]
    #2356507 - 02/19/04 09:22 PM (12 years, 9 months ago)

And I was afraid that they were too wet because you can see the water on the kernels where they touch the glass surface. I always soak for 12-20 hours and drain them overnight. Then I put them in the jars and PC them for about an hour. Usually it works, but this time it did not. Do you think the diluted coffee could have brought in an new vector of contamination ?


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineSuntzu
Geek
 User Gallery

Registered: 10/15/99
Posts: 1,394
Last seen: 1 month, 3 days
Re: Contam on WBS ( pics ) [Re: ragadinks]
    #2356709 - 02/19/04 10:01 PM (12 years, 9 months ago)

Definitely Bacillus in those jars. You should have no problem catching the smell of it by now.
Personally, I've found that the proportion of sunflower seeds can make a difference. I like buying cheap-ass birdseed, and end up scooping many of the sunflower seeds out. If you completely submerge the grain-to-be-used, the sunflower seeds float.
I can't see how the coffee addition would have made the substrate more contam-prone, as Bacillus does fine on any grain. Perhaps the moisture content was compromised in the process? Maybe the pH, but again, the pH of straight grain is conducive to Bacillus. . .


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineExtravagantDream
Beacon in theDarkness
 User Gallery

Registered: 10/24/02
Posts: 1,271
Loc: Somewhere in the Local Su...
Last seen: 9 years, 6 months
Re: Contam on WBS ( pics ) [Re: Suntzu]
    #2356889 - 02/19/04 10:44 PM (12 years, 9 months ago)

I have found that a few sunflower seeds helps out a bit if the grain was too wet. It gives a little space.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineragadinks
MrBeatle
 User Gallery

Registered: 10/20/03
Posts: 1,297
Last seen: 2 years, 8 months
Re: Contam on WBS ( pics ) [Re: Suntzu]
    #2362592 - 02/21/04 04:22 AM (12 years, 9 months ago)

Quote:

I can't see how the coffee addition would have made the substrate more contam-prone, as Bacillus does fine on any grain. Perhaps the moisture content was compromised in the process? Maybe the pH, but again, the pH of straight grain is conducive to Bacillus. . .




In the advanced secion there is a thread "The coffee project". There RoggerRabbit says that he PC's WBS for about 2 hours because coffee has a lot of endospores. As you said: It could also be the PH because coffee has a low PH ( about 5 ).
How can you see from the pic that the moisture is too low ? I always thought too low is better than too high ?


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,121
Loc: my room
Last seen: 2 months, 16 days
Re: Contam on WBS ( pics ) [Re: ragadinks]
    #2362686 - 02/21/04 05:39 AM (12 years, 9 months ago)

You should buy a different kind of birdseed....Ones for Finchs (Finken) or Parrots ( Papagei).
It should be mostly millet, like this:



Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleOldSpice
Geritol Breath...
Female User Gallery

Registered: 08/25/03
Posts: 59,080
Loc: Crankytown, Texas
Post deleted by Administrator [Re: ragadinks]
    #2362756 - 02/21/04 07:29 AM (12 years, 9 months ago)



--------------------
So hard to be ....WDWGFH?
Texas is humongus compared to France
Our Gair, who art in Texas,
Paw Paw be thy Name....
My friends are thirsty


You never see a motorcycle parked outside a Psychiatrist office:biker:


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineragadinks
MrBeatle
 User Gallery

Registered: 10/20/03
Posts: 1,297
Last seen: 2 years, 8 months
Re: Contam on WBS ( pics ) [Re: OldSpice]
    #2362799 - 02/21/04 08:35 AM (12 years, 9 months ago)

Anno:
shit, this seeds were ass cheap. I hoped that it would be ok.

Alounacara:
are the sunflower seeds really that bad? I hoped that PC'ing them for some time would kill all contams.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineSuntzu
Geek
 User Gallery

Registered: 10/15/99
Posts: 1,394
Last seen: 1 month, 3 days
Re: Contam on WBS ( pics ) [Re: ragadinks]
    #2363905 - 02/21/04 03:15 PM (12 years, 9 months ago)

It's impossible to tell at this point whether the original moisture content was too high or too low, as Bacillus growth tends to make things look wetter regardless.  Having jars too wet is one *potential* factor that might favor Bacillus [coffee or no coffee].  When I said the moisture content was possibly 'compromised' I didn't mean 'not enough moisture'.  :smile:
My own 'pet' theory on sunflower seeds is that the texture of the seed coat makes it harder to sterilize;  It's a rough coat, with all sorts of little micro niches. . .millet, on the other hand, has a more smooth 'waxy' coat.  Theoretically, you should be able to get fine results with straight sunflower seeds, if the water content is adjusted accordingly.  Working from personal experience, though, the less the better :wink:
As far as the endospore load, I'd have a hard time believing that coffee beans have significantly/any higher amount than cereal grains. . .who knows, though.


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: ½ Pint Jars, Rye Grain, Wild Bird Seed, pH Test Strips

Mushrooms, Mycology and Psychedelics >> Contamination Forum

Similar ThreadsPosterViewsRepliesLast post
* WBS cake contam HELP!!! (pics) eazygrowin 1,254 8 06/07/05 02:29 PM
by MAIA
* Contam ID ? (Pics) Boris 938 4 12/10/08 08:04 PM
by Boris
* Contam in BRF jars, anybody know what it is? maglight 1,128 7 11/06/07 06:42 PM
by maglight
* Is this contam? check pic CaptBeefheart 808 3 03/05/03 08:05 AM
by MAIA
* Is this contam? (w/ pic) ForbiddenFruit 641 3 08/17/05 11:43 PM
by waixingren
* possible contam.. maybe? (pics inside) thevast 1,064 2 03/17/05 02:05 AM
by MrMaddHatter
* strange red/brown contam w/ pics JT 875 2 07/30/07 11:55 PM
by JT
* Shiitake LC contamed? (No pics) hightimesreader 959 1 01/27/07 09:07 PM
by CureCat

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, cronicr, Pastywhyte
1,344 topic views. 0 members, 3 guests and 1 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Zamnesia.com
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.056 seconds spending 0.003 seconds on 14 queries.