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OfflineKapheen
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Pf tek colonization question(s)....
    #23491444 - 07/30/16 05:59 AM (7 years, 5 months ago)

I have to make this quick, I'm just getting to work but this is something I need to put out there for the more experienced growers to look at...

Okay, so I have 18 jars that were inoculated 31 days ago. I was attempting to wait for them all to completely colonize so that I could fruit them together, for better yields. I started with two strains, Cambodian and albino penis envy. The PE colonized MUCH slower. After 31 days there's still a fair number of jars that aren't completely colonized. I have some that are partially colonized and pinning rather heavily. Should I remove those before they're complete and attempt to fruit? Most of them have reached the bottom of their jars, just not fully covering the bottom portion yet.. a couple more questions, I realize that they're taking much longer than they should be to colonize, what are the possible reasons for this? Also, is it a bad idea to fruit the cakes that are ready to be birthed now, and just add them to the fruiting chamber as they achieve full colonization?

Any thoughts or suggestions that anyone can offer would be greatly appreciated.. thanks.


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Re: Pf tek colonization question(s).... [Re: Kapheen]
    #23491602 - 07/30/16 07:09 AM (7 years, 5 months ago)

Your fine. Be patient. The ones that are starting to pin you can go ahead and birth but make sure to cut off the uncolonized part. Slow growth can be many things. Probably genetics and or temp.


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InvisiblebodhisattaMDiscordReddit
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Re: Pf tek colonization question(s).... [Re: spirit_shadow] * 1
    #23491748 - 07/30/16 08:09 AM (7 years, 5 months ago)

Post pictures


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OfflineKapheen
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Re: Pf tek colonization question(s).... [Re: bodhisatta]
    #23491941 - 07/30/16 09:14 AM (7 years, 5 months ago)

The one is formed to the point where you can see the gills.. the other are still completely white but are obviously visually forming... thoughts ?



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Re: Pf tek colonization question(s).... [Re: Kapheen]
    #23491955 - 07/30/16 09:20 AM (7 years, 5 months ago)

Probably stalling from keeping the foil on during colonization. That and keeping the foil on makes your dry verm barrier get moist which allows bacteria to grow and hinder colonization.

Next time foil off after sterilization so the dry verm barrier can actually work as a filter rather than a wet sponge contamination magnet


Wash off the uncolonized parts and try to fruit the cake you're left with


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Re: Pf tek colonization question(s).... [Re: bodhisatta]
    #23492033 - 07/30/16 09:48 AM (7 years, 5 months ago)

also, those are tall half pints. you want the wide ones. tall ones will often stall, even with very little bacterial problems. with that said, they could also be bacterial, which can cause jars to stall, as well. Oh, but also, PE and APE take longer to colonize and fruit. so any or all of those reasons could be the cause of he stalling.

what youre saying is a mushroom and it's gills(assuming you are referring to second pic) looks kinda strange, to me. looks more like some kind of contam.

also, lots of vendors sell their premade substrate jars with those tall half pints. are these from a vendor or DIY? DIY is cheaper and more reliable.

but i agree with TC, to birth and rinse. when i had those tall jars, and they would stall and start pinning, i just went ahead and birthed them, rinsed the uncolonized sub off, dunk, roll, and fruited in SGFC as normal. still got a few mushrooms from them, but not as much as the ones that finished, and werent as bacterial. if they dont smell like fresh mushrooms, though, then toss them. but not all bacteria/contam will smell. so bad smell can prove contam, but normal smell can not disprove contam.

oh, and yes you can fruit them as they are ready. they dont all have to wait to be fruited at the same time. that's one of the cool things about pf tek.


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Offlinespacechildo
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Re: Pf tek colonization question(s).... [Re: mupetmower]
    #23492063 - 07/30/16 09:57 AM (7 years, 5 months ago)

stalling? looks like a train wreck to me.. trash it and start over.


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OfflineKapheen
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Re: Pf tek colonization question(s).... [Re: mupetmower]
    #23492066 - 07/30/16 09:57 AM (7 years, 5 months ago)

Awesome.. thanks for the replies. You know, I was thinking that it may be a possible contamination as well, but up close it resembles a mushroom.. this is my first attempt so please excuse any ignorance.

Also, yes these are from a vendor. I just figured it's cheap and quick. Less than 35 dollars for 12 prepared jars.

I inoculated 12 more jars recently (3 days ago) with golden teachers and also some b+. Removing the foil from their tops as we speak.


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Re: Pf tek colonization question(s).... [Re: Kapheen]
    #23492132 - 07/30/16 10:14 AM (7 years, 5 months ago)

that is far from cheap. you can spend MUCH less by DIY.

and yeah, space, i was thinking they were pretty bacterial/contam'd, also. but wasnt 100% sure, and wanted to leave the decision to toss up to the more experienced.


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Offlinemrmazdarx9
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Re: Pf tek colonization question(s).... [Re: mupetmower]
    #23492287 - 07/30/16 11:07 AM (7 years, 5 months ago)

Quote:

mupetmower said:
that is far from cheap. you can spend MUCH less by DIY.

and yeah, space, i was thinking they were pretty bacterial/contam'd, also. but wasnt 100% sure, and wanted to leave the decision to toss up to the more experienced.



Plus diy you control the environment there's no telling how sterile the area the jars where prepared in


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OfflineKapheen
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Re: Pf tek colonization question(s).... [Re: mrmazdarx9]
    #23492428 - 07/30/16 12:15 PM (7 years, 5 months ago)

Okay, I birthed 5 of the cakes, including the two in the photos. The one was definitely, without a doubt bacterial.. it smelled pretty damn funky. The rest smelled like fresh mushrooms.. very pleasant.



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Offlinemrmazdarx9
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Re: Pf tek colonization question(s).... [Re: Kapheen]
    #23492436 - 07/30/16 12:18 PM (7 years, 5 months ago)

Need dunking and rolling asap


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OfflineKapheen
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Re: Pf tek colonization question(s).... [Re: mrmazdarx9]
    #23493382 - 07/30/16 06:31 PM (7 years, 5 months ago)

Dunking and rolling as in dunking in water and rolling in vermiculite? I read that that wasn't necessary as long as i rehydrate them in between fruitings.


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OfflineMycologist217
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Re: Pf tek colonization question(s).... [Re: Kapheen]
    #23493410 - 07/30/16 06:41 PM (7 years, 5 months ago)

Dunk and roll your cakes bro


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OfflineKapheen
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Re: Pf tek colonization question(s).... [Re: Mycologist217]
    #23493429 - 07/30/16 06:48 PM (7 years, 5 months ago)

Okay.. but as I said in my last post, I read that that wasn't necessary. I don't have any vermiculite currently. I can get some tomorrow.. I think every place that sells it is closed right now. Will they be okay for the night?


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Re: Pf tek colonization question(s).... [Re: Kapheen]
    #23493448 - 07/30/16 06:55 PM (7 years, 5 months ago)

Put them in water right now (dunk) leave them in the water for the night until you have the verm....then take them out of dunk water and roll them in verm....wherever you read that it isn't necessary was wrong...,just wrong.


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OfflineKapheen
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Re: Pf tek colonization question(s).... [Re: Mycologist217]
    #23493644 - 07/30/16 08:01 PM (7 years, 5 months ago)

I made it to Home Depot in time to grab a bag of vermiculite, then stopped at walmart and grabbed a bunch of zip lock bags. They're currently all submerged in water in their own individual bags. Thanks for the tip Mycologist.


Edited by Kapheen (07/30/16 09:33 PM)


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OfflineRealmLAir
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Re: Pf tek colonization question(s).... [Re: Kapheen]
    #23493871 - 07/30/16 09:22 PM (7 years, 5 months ago)

currently following this thread as Ive just started the same project. Innoc'd BRF cakes on 7/15 and have yet to see any sign of growth. Albeit, after reading this thread I DID barely just remove the foil from 4 out of 6 of my jars. the dry verm layer is now exposed, no foil whatsoever.


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OfflineKapheen
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Re: Pf tek colonization question(s).... [Re: RealmLAir]
    #23493896 - 07/30/16 09:31 PM (7 years, 5 months ago)

Hmm.. I really don't know for certain, but of the 4 strains that I've used I've seen growth from all of them within 2 weeks, most of them (other than the APE) showed growth within 4 days. This is just from my experience. But I would think you should be showing some signs of growth...


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Re: Pf tek colonization question(s).... [Re: Kapheen]
    #23493902 - 07/30/16 09:34 PM (7 years, 5 months ago)

Use those bags and put your cakes in, add Verm, and shake well. I find that it sticks better than simply "rolling"

Shake one cake at a time.


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