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MykoMykoAnay
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Registered: 03/18/16
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55 gallons of LC
#23491152 - 07/30/16 01:51 AM (7 years, 5 months ago) |
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I have a 55 gallon steel drum and I need some outside opinions on my logic. The logic of how I'm going about this, not why. My family have some land and my uncle keeps live stock on it. Every so often he moves the cattle from one pasture to another. In one of the pastures the water tank is right under a small grove of huge, sprawling oak trees. Naturally the cows like to hang out in the shade on hot days and also spend a lot of time drinking. These two variables mean there's a lot of shit in a relatively small area. I am thinking about raking the area around the water tanks in the other three pastures and bringing it to the shaded site with our tractor. All together I bet there would be about a 2" layer of shit over an area with the dimensions of ~75'x150', or 11,250 sq ft.
Now to the how of it.
I have a black, steel, 55 gal drum that I intend on fitting with two Plexiglas windows directly across from each other so I can keep an eye on stuff, sealed with silicone. There are two larger holes in the the lid that will be utilized for GE and hooking the barrel to the sprayer. The bigger of the two holes will be used to GE as it is almost the perfect size for a filter disc and I really don't need a bid diameter hole for spraying but I do need the threading and it is closer to he diameter of the hose. Less messing around with having to step down in diameters later. I would insert a smooth interior diameter male end into the hole and then insert a pvc pipe that was a small enough diameter that I could use it as a sort of swizzle stick. I'd have a booth that was secured by the male end and came up and held with a hose clamp on the pvc pipe. I'll be adding an injection port just like on a little jar and will be pumping a Qt jar of LC in to the barrel using a 100 ml syringe. 2.75 gallons of Karo will be used with 50 gallons of mineral water. Before I add these two together I would set the barrel on something, haven't figured this out yet, perhaps at least a foot off the ground. Then build a fire around the barrel and boil the mixture for at least an hour. Not sure how to protect the Plexiglas either. After cooling for 24 hrs I'd move the barrel back into my garage and knock it up. Monitored with the windows installed and then when the day comes hook it up to the PTO sprayer on the tractor and spread my seed.
Any flaws?
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mrmazdarx9
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Registered: 05/15/16
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What would you need 55 gallons for I'd imagine you could do acres with that much You only need 1cc for a half pint jar 250036cc is roughly 55 gallon (UK) would do 62509 pints worth of substrate lol
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Edited by mrmazdarx9 (07/30/16 02:08 AM)
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Flaws/questions to ask before
1) how many successful lcs have you done before? 50? How many jars have you successfully fruited made from lc innoc? 500 quarts?
2) how are you making sure the lc is sterile, specifically, beyond 'a fire under it', do you have a pressure gauge on the barrel?
3) bags would probably be better suited so you can see visually see growth. The plexiglass window is not goin to be verifying every gallon of the lc is clean, people fuck that up with pints of lc and your talking about visually checking 50 gallons in one container. impossible.
4) all your eggs in one basket. The cow farm doesnt have one cow that weighs 55 tons, it has 55 cows that weigh a ton each. So if one thing fucks up one cow their entire farm is not lost. Ever heard the expression betting the farm?
5) lc goes to grain, then substrate, never seen it go lc>> substrate except in smaller sterile containers. So im pretty sure its a wild goose chase
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MykoMykoAnay
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Registered: 03/18/16
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Bragging rights. Why do people put a V8 engine on a motorcycle frame? I see what you're saying though. With the help of Google I think I've figure out what exactly it would take, if I felt like being reasonable. There are approximately 29 cubic inches in a pint. So, we need to convert ft to inches. (75'x150') 12" x 2"deep = 9,310 Pints, 18,620 half pint jars. 3785.41 milliliters in a gallon and one milliliter per half pint leads us to 18,620/3785.41 = 4.9188859331 gallons.
Also, I came up with 208197.55 half pint jars with 55 gallons of LC.
While I was doing this I was day dreaming about doing this with a wood lover and spraying the woods, all the woods, every where.
-------------------- "He who seeks freedom for anything but freedom's self is made to be a slave." Old Regime (1856)
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MykoMykoAnay
Stranger


Registered: 03/18/16
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Quote:
filthyknees said: Flaws/questions to ask before
1) how many successful lcs have you done before? 50? How many jars have you successfully fruited made from lc innoc? 500 quarts?
2) how are you making sure the lc is sterile, specifically, beyond 'a fire under it', do you have a pressure gauge on the barrel?
3) bags would probably be better suited so you can see visually see growth. The plexiglass window is not goin to be verifying every gallon of the lc is clean, people fuck that up with pints of lc and your talking about visually checking 50 gallons in one container. impossible.
4) all your eggs in one basket. The cow farm doesnt have one cow that weighs 55 tons, it has 55 cows that weigh a ton each. So if one thing fucks up one cow their entire farm is not lost. Ever heard the expression betting the farm?
5) lc goes to grain, then substrate, never seen it go lc>> substrate except in smaller sterile containers. So im pretty sure its a wild goose chase
#1 don't see the relevance
#2 good point, with the threaded openings i could easily adapt a pressure gauge to fit the smaller hole
#3 never though about bags...maybe. I guess more than one set of windows would need to be installed but I think you're over stating this specific problem. bulk liquid bags
#4 a little redundant
#5 spraying liquid culture
Thank you for the input. This idea has my head swimming with the possibilities.
-------------------- "He who seeks freedom for anything but freedom's self is made to be a slave." Old Regime (1856)
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mrmazdarx9
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Registered: 05/15/16
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Quote:
MykoMykoAnay said: Bragging rights. Why do people put a V8 engine on a motorcycle frame? I see what you're saying though. With the help of Google I think I've figure out what exactly it would take, if I felt like being reasonable. There are approximately 29 cubic inches in a pint. So, we need to convert ft to inches. (75'x150') 12" x 2"deep = 9,310 Pints, 18,620 half pint jars. 3785.41 milliliters in a gallon and one milliliter per half pint leads us to 18,620/3785.41 = 4.9188859331 gallons.
Also, I came up with 208197.55 half pint jars with 55 gallons of LC.
While I was doing this I was day dreaming about doing this with a wood lover and spraying the woods, all the woods, every where.
I'm using UK measurements lol I'm not shitting on your idea go for it if it worked it would be cool to see like something outta Alice in wonderland
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MykoMykoAnay
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Registered: 03/18/16
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That's what I'm thinking man. Or wizard of Oz and instead of a poppy field a fucking forest of mushrooms.
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mrmazdarx9
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Registered: 05/15/16
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Quote:
MykoMykoAnay said: That's what I'm thinking man. Or wizard of Oz and instead of a poppy field a fucking forest of mushrooms.
Aha yeah that would be awesome
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MykoMykoAnay
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Registered: 03/18/16
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I think my math was wrong. There are 144 sq inches in a sq foot so it would be (75'x150')144"x2"=3,240,000 cubic inches divided by 29 to give us the pints, which come out to 111724.13 and 223448.27 for half pints. 3785.41 milliliters in a gallon, 223448.27 divided by 3785.41 = 59.02 gallons. There are 43,560 sq ft in an acre and we just calculated that we need 59 gallons for 11,250 sq ft. 43,560/11,250=3.872; 3.872x59=228.448 gallons per acre@ assuming you're spraying for 2" colonization based on ml per half pint.
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tump
ban the undead



Registered: 03/17/16
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Ok better plan for 55gallons of lc. This plan a little more theasable. First its going to take a dick year to colonies of myc for that big amount. So in the meant time bluid a stream sterilizer barrels and then buy grow bags. Run 30 gallons of cracked corn spawn a day for 30 days. Plus a barn to store these bags also for drying later. Once the lc is grown and stired with huge magnet stirer. Make a pressure needle to sprays 25 ml of lc per gallon bag. Let it colonies through out. Then plant them in your cow shit field. Later when you have the trouble drying. A few thoumd pound of shrooms use the barn you bluiding. Then invite all of us over to party for a week.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Quote:
filthyknees said: Flaws/questions to ask before
1) how many successful lcs have you done before? 50? How many jars have you successfully fruited made from lc innoc? 500 quarts?
2) how are you making sure the lc is sterile, specifically, beyond 'a fire under it', do you have a pressure gauge on the barrel?
3) bags would probably be better suited so you can see visually see growth. The plexiglass window is not goin to be verifying every gallon of the lc is clean, people fuck that up with pints of lc and your talking about visually checking 50 gallons in one container. impossible.
4) all your eggs in one basket. The cow farm doesnt have one cow that weighs 55 tons, it has 55 cows that weigh a ton each. So if one thing fucks up one cow their entire farm is not lost. Ever heard the expression betting the farm?
5) lc goes to grain, then substrate, never seen it go lc>> substrate except in smaller sterile containers. So im pretty sure its a wild goose chase
LC's meant for outside don't really need to be sterile. I have seen a post from a woodlover grower saying how he just drops spores or a clone in an LC and let it grow out, contams and all. Once that is poured outside, the mycelium will be able to outgrow any contamination more often than not.
I do agree that 55gallons is too much though, if it were me, I would just do quart or half gallon jars with LC and just pour that around where you want the shrooms to grow. You don't have to cover every square inch of the place with LC, the mycelium will grow to the spots where the LC didn't hit. Pouring it in lines a foot apart or zigzag motions would still work great.
If it is already summer over there, don't expect anything this year though, it might need time to grow and consolidate (even more important) before it fruits. This means that even if you soak the whole field with LC, it will still need time to colonize the soil and consolidate so the amount of LC is not as important as you seem to think it is. Most folks who see fruits right after spawning outside get them because the mycelium has already been grown on a bulk sub, consolidated and usually also fruited before it was buried. People who spawn woodlover spawn outside often have to wait 1-2 years before they see fruits.
Another concern is that since this part of the field will be packed with cows, the fresh poo and urine might hinder the mycelium from fruiting. Homegrowers who use poo only use field aged or composted manure since these will be already leached from ammonia. This is just a guess/me brainstorming though, I'm not sure if this will be a problem or not. The mycelium might not like having a shitload of cows pissing on the patch.
I wish you luck with this though.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
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Quote:
MykoMykoAnay said:
#1 don't see the relevance
You don't see the relevance of whether you have successfully made and used an LC before, to the idea of making 55 gallons of the shit?
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spirit_shadow
Feature not a bug



Registered: 08/15/11
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Re: 55 gallons of LC [Re: Inocuole]
#23491579 - 07/30/16 07:00 AM (7 years, 5 months ago) |
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Careful not to accidentally kill all your livestock :/
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weetsie
unlicensed tub surgeon



Registered: 05/08/11
Posts: 572
Loc: United Kingdom
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I would go for some kind of slurry instead, blend up some colonized substrate and spread that.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: 55 gallons of LC [Re: weetsie] 1
#23491704 - 07/30/16 07:52 AM (7 years, 5 months ago) |
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http://discussions.probrewer.com/showthread.php?55700-Laboratory-propegation-and-Brewery-propegation-of-yeast-(SOPs)
Yeast is just single celled fungi. Same thing as a LC but a hair different. You'll be able to use the methods in that link to cross over
For 55gal you're going to need to keep it aerobic you will need to pump in sterile air or O2. I suggest air since you would have to regulate O2 to not become toxic. With just air no worry.
The air also serves as agitation to keep growth from getting all clumpy
You're going to have to inoculate then 55g LC with a large amount of LC you'll want to use at least a gallon if not more to inoculate.
Not using enough inoculant to start the 55g LC means a huge lag phase and also a huge opportunity for contamination to grow in the media while the mycelium catches up
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Quote:
MykoMykoAnay said:
Quote:
filthyknees said: Flaws/questions to ask before
1) how many successful lcs have you done before? 50? How many jars have you successfully fruited made from lc innoc? 500 quarts?
2) how are you making sure the lc is sterile, specifically, beyond 'a fire under it', do you have a pressure gauge on the barrel?
3) bags would probably be better suited so you can see visually see growth. The plexiglass window is not goin to be verifying every gallon of the lc is clean, people fuck that up with pints of lc and your talking about visually checking 50 gallons in one container. impossible.
4) all your eggs in one basket. The cow farm doesnt have one cow that weighs 55 tons, it has 55 cows that weigh a ton each. So if one thing fucks up one cow their entire farm is not lost. Ever heard the expression betting the farm?
5) lc goes to grain, then substrate, never seen it go lc>> substrate except in smaller sterile containers. So im pretty sure its a wild goose chase
#1 don't see the relevance
#2 good point, with the threaded openings i could easily adapt a pressure gauge to fit the smaller hole
#3 never though about bags...maybe. I guess more than one set of windows would need to be installed but I think you're over stating this specific problem. bulk liquid bags
#4 a little redundant
#5 spraying liquid culture
Thank you for the input. This idea has my head swimming with the possibilities.
this is a garbage idea and you are going to fail 100%. not because this is a difficult task, but because its an impossible one, especially for you.
1) if you don't see the relevance, just abandon this whole fucking project man. if you haven't made a normal LC, you are never going to make 55 gallons of it, ever.
2) you don't even understand the basics of sterilization it seems
3) I think you're overstating your skills and understanding of mycology
4) learn to accept good advise and not be such a blowhard
5) if thats your "proof" for this method, then you are totally fucked
Edited by Munchauzen (07/30/16 08:28 AM)
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Urmomma
Strangler
Registered: 07/28/16
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Why not put spores in the cow's drinking water?
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Re: 55 gallons of LC [Re: Urmomma]
#23491804 - 07/30/16 08:29 AM (7 years, 5 months ago) |
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Quote:
Urmomma said: Why not put spores in the cow's drinking water?
this has a much better chance of working than bulk lc to substrate...
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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If you use the 55g drum LC there's no way it's going to work for anything but spraying on straw and making outdoor beds.
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MykoMykoAnay
Stranger


Registered: 03/18/16
Posts: 88
Last seen: 3 years, 4 months
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Quote:
weetsie said: I would go for some kind of slurry instead, blend up some colonized substrate and spread that.
Interesting, it seems like this would help with the colonization times as well. I'd have to find a different method of spraying. Something that can handle fairly large particulates.
Quote:
Inocuole said:
Quote:
MykoMykoAnay said:
#1 don't see the relevance
You don't see the relevance of whether you have successfully made and used an LC before, to the idea of making 55 gallons of the shit?
This is why...
Quote:
Supalemonhaze said:
LC's meant for outside don't really need to be sterile. I have seen a post from a woodlover grower saying how he just drops spores or a clone in an LC and let it grow out, contams and all. Once that is poured outside, the mycelium will be able to outgrow any contamination more often than not.
How about pressure cooking the LC in the pot rather than in jars then create a vacuum in a five gallon bucket that has been sterilized and let the vacuum transfer the liquid via the port for the pressure gauge, or perhaps I can tap and thread a specialty port with a siphon to the bottom of the pot? Use an air house coupling or something.
That being said, I'm starting to lean towards a woods full of shrooms. Using a large expedition pack I could easily, and discretely spray family member's woods and public land. Anyone know of a strain that is hardy to USDA zone 3?
Anyone have a clever version of Johnny Apple Seed for this endeavour?
-------------------- "He who seeks freedom for anything but freedom's self is made to be a slave." Old Regime (1856)
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