Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinepichardo
Pich
Male User Gallery

Registered: 08/31/10
Posts: 172
Loc: Ensenada, B.C. México.
Last seen: 1 year, 3 months
Liquid culture bottle.
    #23489377 - 07/29/16 01:49 PM (7 years, 5 months ago)



It-s polycarb, I have doubt about if it works for liquid culture mycelium...


--------------------



Extras: Filter Print Post Top
Invisibleweetsie
unlicensed tub surgeon
 User Gallery


Registered: 05/08/11
Posts: 572
Loc: United Kingdom Flag
Re: Liquid culture bottle. [Re: pichardo]
    #23490191 - 07/29/16 07:21 PM (7 years, 5 months ago)

I see no reason why not.


--------------------
Active grow logs: Oysters on Straw Pellets
Trade list


Extras: Filter Print Post Top
Offlinetump
ban the undead
 User Gallery


Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
Re: Liquid culture bottle. [Re: weetsie]
    #23490580 - 07/29/16 09:56 PM (7 years, 5 months ago)

If you can pc it and get the culture out with a needle it be perfect. Lc aren't picky of bottles, jars , 2 liters. But if you can't get it out cleanly its worthless


Extras: Filter Print Post Top
Offlineleschampignons
Biochemistry + Mycology
Male User Gallery


Registered: 08/30/13
Posts: 1,583
Loc: NY/NJ/ME Flag
Last seen: 4 days, 5 hours
Re: Liquid culture bottle. [Re: pichardo]
    #23490588 - 07/29/16 09:59 PM (7 years, 5 months ago)

I don't think polycarbonate will withstand heat sterilization without melting. So assuming it comes sterile it would only be good for one use.


--------------------
leschampignons Trade List


Extras: Filter Print Post Top
Offlinepichardo
Pich
Male User Gallery

Registered: 08/31/10
Posts: 172
Loc: Ensenada, B.C. México.
Last seen: 1 year, 3 months
Re: Liquid culture bottle. [Re: leschampignons]
    #23512747 - 08/05/16 01:02 PM (7 years, 5 months ago)

Polycarbonate its specially designed for that.
My doubt was for the CO2 on medium.


--------------------



Extras: Filter Print Post Top
Offlinepichardo
Pich
Male User Gallery

Registered: 08/31/10
Posts: 172
Loc: Ensenada, B.C. México.
Last seen: 1 year, 3 months
Re: Liquid culture bottle. [Re: pichardo]
    #23513149 - 08/05/16 03:01 PM (7 years, 5 months ago)

So here it is.


Looks good, its been 6 days of incubation, stir bar mantain at 200rpm, and did vortex effect(stirrer at max) for 3min everyday for oxygen.

I will inoculate some petri dishes and grain on 07/08/16


--------------------



Edited by pichardo (08/05/16 03:14 PM)


Extras: Filter Print Post Top
Offlineinvitro


Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 21 days
Re: Liquid culture bottle. [Re: pichardo]
    #23513277 - 08/05/16 03:41 PM (7 years, 5 months ago)

I'm fairly sure the vortex effect is not needed, the 200 continuous rpm should be enough to
bring the liquid to oxygen saturation.


Extras: Filter Print Post Top
OfflineFreeTheSoul
The wonderer.
Registered: 01/04/14
Posts: 2,297
Last seen: 2 years, 2 months
Re: Liquid culture bottle. [Re: invitro]
    #23513659 - 08/05/16 05:45 PM (7 years, 5 months ago)

How did you inoculate the lc? Also do you have a sfd piece on top of the lc container? Just wondering.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies


Similar ThreadsPosterViewsRepliesLast post
* Panellus stipticus and liquid culturing techniques Blue Helix 3,317 11 09/25/05 11:57 AM
by GourmetMushroom
* Oysters, coffee grounds, and liquid culture psyconaut 5,268 10 11/07/02 12:51 PM
by psyconaut
* Which gourmets/medicinals do well in liquid culture? GnuBobo 2,324 6 04/25/05 10:06 AM
by mule
* Vendor Folks! Black Poplar liquid culture medium GnuBobo 1,008 1 11/20/05 08:01 AM
by falcon
* Liquid culture (dextrose) question MushMushi 1,532 2 10/01/02 01:14 PM
by MushMushi
* When are reishi liquid cultures ready? Bobo 1,034 2 12/19/19 08:50 PM
by slones
* Cultivating yeast cultures. Baby_Hitler 2,531 13 07/14/04 12:54 PM
by blackmold
* Got some Panellus stipticus (glowing) cultures. Looking for White Trumpet (Pleurotus eryngii) GourmetMushroom 1,389 4 01/18/24 06:34 PM
by madcap93

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, Pastywhyte, Forrester, Stromrider, SHROOMSISAY01
389 topic views. 2 members, 11 guests and 2 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.024 seconds spending 0.007 seconds on 14 queries.