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OfflineTerpfreak
❀Terpenes❀
Female


Registered: 07/08/16
Posts: 1,065
Loc: Land Of Ooo
Last seen: 5 years, 10 months
Noob problems ?
    #23485176 - 07/28/16 10:05 AM (7 years, 6 months ago)

Im pretty sure all I'm growing in this jar is bacteria. Any information is appreciated!
WBS sterilized .
Inoculated next day, incubated in small cardboard box at 76-86
Ten days in. 
new cultivator obviously, taking every possible precaution besides a glovebox.
However I inoculated in a sterile canna grow environment with all fans off.




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InvisibleKush_Zombie
smug piece of shit
 User Gallery


Registered: 10/22/14
Posts: 4,793
Re: Noob problems ? [Re: Terpfreak] * 1
    #23485187 - 07/28/16 10:08 AM (7 years, 6 months ago)

Yeah those definitely don't look right.

Nice nails.

Also, I prefer using coffee containers to burn 2 holes into a clear plastic tub. Regardless on what you use though, you should lay a wet towel at the bottom for contams to fall and stick to and spray the inside of your SAB with soapy water to also make contams stick.

And the pic where you're holding two of them, the one on the left looks like it might be fine.


--------------------
How to get started in bulk:
Presto 23-Quart Pressure Cooker
BOD's Simple as FUCK Still Air Box
PastyWhyte's Easy Agar Tek
Munchauzen's Cultivation Video Series
How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs)
What is G2G? (Grain-to-Grain)
Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary)
Spitball's Monotub Tek (A liner isn't necessary but is useful)
Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.


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OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 8 days
Trusted Cultivator
Re: Noob problems ? [Re: Kush_Zombie]
    #23485195 - 07/28/16 10:12 AM (7 years, 6 months ago)

the one you are holding is trash


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineAgarStudent
Noob
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Registered: 01/25/15
Posts: 550
Last seen: 2 years, 10 days
Re: Noob problems ? [Re: Terpfreak]
    #23485197 - 07/28/16 10:13 AM (7 years, 6 months ago)

Hmm that seems like pretty fast growth in ten days.  If it colonises too fast then be skeptical.  If it is a strain of cubensis, normally it would take four weeks or so to colonise at a nice toasty temp.  On the first image the partially colonised area appears to lack defined boundaries and has kind of blurred edges.  I have my doubts that what you have growing in there is what you want.
Mycelium typically has very sharp bounded edges and strands of growth can be seen if you look closely.


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Why reinvent the wheel when someone here has already done the science?



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OfflineTerpfreak
❀Terpenes❀
Female


Registered: 07/08/16
Posts: 1,065
Loc: Land Of Ooo
Last seen: 5 years, 10 months
Re: Noob problems ? [Re: Kush_Zombie]
    #23485230 - 07/28/16 10:24 AM (7 years, 6 months ago)

Quote:

cronicr said:
the one you are holding is trash



Quote:

Kush_Zombie said:
Yeah those definitely don't look right.

Nice nails.

Also, I prefer using coffee containers to burn 2 holes into a clear plastic tub. Regardless on what you use though, you should lay a wet towel at the bottom for contams to fall and stick to and spray the inside of your SAB with soapy water to also make contams stick.

And the pic where you're holding two of them, the one on the left looks like it might be fine.






Thanks for the quick replies everyone. It is a cube , but the species is known to be a fast colonizer. ( pardon my shit myco vocab). 

Okay so my suspicion is cleared, what can I do at this point to insure the others survive? 
I've already separated the messed up one and put the others in a plastic bin with a blanket over her, in the grow room where temps should stay around 80-85.  I'm assuming all I can do now is hope, pray to the myco gods, go over my notes to see where I went wrong and do better next time?


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InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Noob problems ? [Re: Terpfreak]
    #23485246 - 07/28/16 10:30 AM (7 years, 6 months ago)

What do you use for a filter? If you got filters that are good, the other jars will be fine. The one you're holding has pin mold galore going on there. The one on the left looks OK so far


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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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Invisiblemupetmower
Mower of Muppets
Male User Gallery


Registered: 03/29/16
Posts: 3,036
Loc: Here and There
Re: Noob problems ? [Re: Terpfreak]
    #23485247 - 07/28/16 10:31 AM (7 years, 6 months ago)

pin mold. ew.


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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OfflinecronicrFacebook
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Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 8 days
Trusted Cultivator
Re: Noob problems ? [Re: Mad Season] * 1
    #23485252 - 07/28/16 10:31 AM (7 years, 6 months ago)

save your coffee filters for your coffee pot.


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineTerpfreak
❀Terpenes❀
Female


Registered: 07/08/16
Posts: 1,065
Loc: Land Of Ooo
Last seen: 5 years, 10 months
Re: Noob problems ? [Re: cronicr]
    #23485273 - 07/28/16 10:41 AM (7 years, 6 months ago)

I used tyvek and coffee filters. What would be the perfect alternative?
Quote:

cronicr said:
save your coffee filters for your coffee pot.





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Edited by Terpfreak (07/28/16 10:42 AM)


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InvisibleKush_Zombie
smug piece of shit
 User Gallery


Registered: 10/22/14
Posts: 4,793
Re: Noob problems ? [Re: Terpfreak]
    #23485277 - 07/28/16 10:43 AM (7 years, 6 months ago)

Synthetic Filter Discs would be ideal if you have money to spend on them. They're re-usable and easier than making tyvek lids.


--------------------
How to get started in bulk:
Presto 23-Quart Pressure Cooker
BOD's Simple as FUCK Still Air Box
PastyWhyte's Easy Agar Tek
Munchauzen's Cultivation Video Series
How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs)
What is G2G? (Grain-to-Grain)
Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary)
Spitball's Monotub Tek (A liner isn't necessary but is useful)
Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.


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OfflineTerpfreak
❀Terpenes❀
Female


Registered: 07/08/16
Posts: 1,065
Loc: Land Of Ooo
Last seen: 5 years, 10 months
Re: Noob problems ? [Re: Kush_Zombie]
    #23485286 - 07/28/16 10:45 AM (7 years, 6 months ago)

Thank you, anything else? I would look into a more sanitary method but I don't know where to begin.


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Invisibleh0ldthedoor
HODOR
I'm a teapot User Gallery


Registered: 06/25/16
Posts: 510
Loc: North of The Wall
Re: Noob problems ? [Re: Kush_Zombie]
    #23485368 - 07/28/16 11:06 AM (7 years, 6 months ago)

Quote:

Kush_Zombie said:
Synthetic Filter Discs would be ideal if you have money to spend on them.




There's a guide somewhere on here about making SFD's from polyfill. Pretty sure materials were just a bag of polyfill, an iron, an ironing board, something to use as a template and something to cut the disc out with. This should be considerably cheaper, assuming OP already has an iron.


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


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OfflineTerpfreak
❀Terpenes❀
Female


Registered: 07/08/16
Posts: 1,065
Loc: Land Of Ooo
Last seen: 5 years, 10 months
Re: Noob problems ? [Re: h0ldthedoor]
    #23485402 - 07/28/16 11:14 AM (7 years, 6 months ago)

Quote:

h0ldthedoor said:
Quote:

Kush_Zombie said:
Synthetic Filter Discs would be ideal if you have money to spend on them.




There's a guide somewhere on here about making SFD's from polyfill. Pretty sure materials were just a bag of polyfill, an iron, an ironing board, something to use as a template and something to cut the disc out with. This should be considerably cheaper, assuming OP already has an iron.




Would a "tshirt heat press" work? (Haha)

I forgot to mention the jars are also polyfilled under the coffee filter as well.
If I made the SFDs would that be all or would that be used under the lid with polyfilled hole.

EDIT:  Is it too late to add some humidity back into the jars at this point?  Is it necessary?  - I was going to leave them be until tub time. Thanks again everyone


--------------------


Edited by Terpfreak (07/28/16 11:21 AM)


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Invisibleh0ldthedoor
HODOR
I'm a teapot User Gallery


Registered: 06/25/16
Posts: 510
Loc: North of The Wall
Re: Noob problems ? [Re: Terpfreak]
    #23485409 - 07/28/16 11:17 AM (7 years, 6 months ago)

A tshirt heat press would probably work. If that Homemade SFD thread was easier to find, I'd provide a link.


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


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InvisibleKush_Zombie
smug piece of shit
 User Gallery


Registered: 10/22/14
Posts: 4,793
Re: Noob problems ? [Re: Terpfreak]
    #23485411 - 07/28/16 11:17 AM (7 years, 6 months ago)

Idk the method he's talking about but I would assume a T-shirt press would work fine if a regular iron does.

With a SFD you just drill 2 different 1/4" holes in the lid for gas exchange, and then place the SFD on top of the lid and then screw your ring on top of the lid/SFD.


--------------------
How to get started in bulk:
Presto 23-Quart Pressure Cooker
BOD's Simple as FUCK Still Air Box
PastyWhyte's Easy Agar Tek
Munchauzen's Cultivation Video Series
How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs)
What is G2G? (Grain-to-Grain)
Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary)
Spitball's Monotub Tek (A liner isn't necessary but is useful)
Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.


Extras: Filter Print Post Top
InvisibleKush_Zombie
smug piece of shit
 User Gallery


Registered: 10/22/14
Posts: 4,793
Re: Noob problems ? [Re: Kush_Zombie]
    #23485415 - 07/28/16 11:18 AM (7 years, 6 months ago)



--------------------
How to get started in bulk:
Presto 23-Quart Pressure Cooker
BOD's Simple as FUCK Still Air Box
PastyWhyte's Easy Agar Tek
Munchauzen's Cultivation Video Series
How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs)
What is G2G? (Grain-to-Grain)
Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary)
Spitball's Monotub Tek (A liner isn't necessary but is useful)
Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.


Extras: Filter Print Post Top
Invisibleh0ldthedoor
HODOR
I'm a teapot User Gallery


Registered: 06/25/16
Posts: 510
Loc: North of The Wall
Re: Noob problems ? [Re: Kush_Zombie]
    #23485426 - 07/28/16 11:22 AM (7 years, 6 months ago)

Quote:

Kush_Zombie said:
Would this be it? https://www.shroomery.org/forums/showflat.php/Number/20115107/fpart/1/vc/1





That be it! :thumbup:


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


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OfflineTerpfreak
❀Terpenes❀
Female


Registered: 07/08/16
Posts: 1,065
Loc: Land Of Ooo
Last seen: 5 years, 10 months
Re: Noob problems ? [Re: Kush_Zombie]
    #23485435 - 07/28/16 11:24 AM (7 years, 6 months ago)

I couldn't be more gracious! Or aggravated with myself ! thanks for the tips and your time I really really appreciate it.


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InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: Noob problems ? [Re: Terpfreak]
    #23485480 - 07/28/16 11:40 AM (7 years, 6 months ago)

if you cut the SFD up and just silicone it over a 1/4-3/8" hole you can get 12 of them from one disk. they're outrageously cheap. for 10$(including shipping) you can get enough quality SFDs for 24 jars if not more.


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OfflineTerpfreak
❀Terpenes❀
Female


Registered: 07/08/16
Posts: 1,065
Loc: Land Of Ooo
Last seen: 5 years, 10 months
Re: Noob problems ? [Re: bodhisatta]
    #23485709 - 07/28/16 12:55 PM (7 years, 6 months ago)



These are the supposedly clean jars. I'm worried. ??


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