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Invisibleh0ldthedoor
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how do these jars look? pf tek, brf cakes
    #23483240 - 07/27/16 06:20 PM (7 years, 6 months ago)

First timer, here are some jars that were inoculated on Sunday morning. Would appreciate feedback and advice, especially if anything looks wrong. The jar that is circled looks different than the rest, maybe the start of some mycelium? Would have snapped photos of all jars, but did not want to disturb them.



--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


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Invisiblemupetmower
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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor]
    #23483245 - 07/27/16 06:21 PM (7 years, 6 months ago)

yup. thats myc starting to grow in the circled pic. :thumbup:

now just gotta play the waiting game.


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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Invisibleh0ldthedoor
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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor]
    #23483291 - 07/27/16 06:34 PM (7 years, 6 months ago)

:zomgzomgzomg:


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


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Offlinelocallorax
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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor]
    #23483313 - 07/27/16 06:42 PM (7 years, 6 months ago)

your jars look properly mixed. this is pleasing


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Invisibleh0ldthedoor
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Re: how do these jars look? pf tek, brf cakes [Re: locallorax]
    #23484725 - 07/28/16 07:10 AM (7 years, 6 months ago)

Thank you!

Checked them this morning and another jar has a little spot of something going on now too.

Here's the jar that is circled in the first post


Here is the other jar


It is still really early, but does the moisture look good? After checking some pf tek grow logs, the moisture on the inside of the jar has me a little worried..

Go easy on me if posting pics this early and asking for advice is poor form, still learning and really worried about these little guys...


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


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Invisiblemupetmower
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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor]
    #23484779 - 07/28/16 07:41 AM (7 years, 6 months ago)

they look fine. a little moisture on the inside is normal. especially once the myc starts growing, it will create more moisture.

try to leave them alone, and only check on them weekly or so, until they are getting really close to finished. this way you arent moving them too much and it should help the anxiety/worry. maybe start some more projects, like getting into agar or something, to keep you occupied and away from the colonizing jars =p

or make your SGFC if you havent already.

looks good so far, though.


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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Invisibleh0ldthedoor
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Re: how do these jars look? pf tek, brf cakes [Re: mupetmower]
    #23486364 - 07/28/16 04:09 PM (7 years, 6 months ago)

Got the tub, planning on how to build it now.

Dimensions of the tub are 26 1/2" L x 16" W x 12 1/4" H

The initial plan is to make 20 holes on the long sides, one every 5 1/3" across spaced 3 1/16" apart, make 12 holes on the short sides, one every 3" across spaced 3 1/16" apart, and make 20 holes on the bottom and top sides, one every 5 1/3" across spaced 4" apart. The holes would all be 1/4".

The inside would have about 4" of perlite, with the BRF cakes sitting on foil sheets slightly larger than the cakes themselves. There would also be an equivalent 60w CFL bulb over the tub on a 12/12 timer. The tub would also be resting on something to elevate it off of whatever it's placed on (counter, shelf, box, whatever the case) by about 1" or 2" (depending on what's lying around).

Points of concern are the exact number of holes and the size of holes (pf tek says to use uniform 1/4" holes, comparing the perlite to a 1/4" hole, it looks like the perlite will fall through, how can one prevent this from happening? is screen a viable solution?).

How does this sound?

Mind you all measurements are approximate, this is nothing but a sketch in my mind.


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


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Offlinezeric
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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor]
    #23486533 - 07/28/16 05:00 PM (7 years, 6 months ago)

Do you have a non-inoculated jar for easy mould detection?

The jars look fine tough :wink:


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Invisiblemupetmower
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Re: how do these jars look? pf tek, brf cakes [Re: zeric]
    #23486549 - 07/28/16 05:05 PM (7 years, 6 months ago)

this is all you need to know.. the exact number of holes you need is however many holes it takes to drill 14in in a 2x2in grid on all sides.

why are you planning all these crazy dimensions for the holes? just make a proper SGFC, following that tek.

the perlite wont fall through. a few pieces will when you first put it in, but then it will stop, and the rest will stay just fine.

also, i would try to get it more than 2in from the floor/table. try for at least 4-6in if possible. you could use jars or canned soup/veggies, cups, etc. and ideally, you want at least a foot on all sides from walls/obstructions.


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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Offlinezeric
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Re: how do these jars look? pf tek, brf cakes [Re: zeric]
    #23486553 - 07/28/16 05:05 PM (7 years, 6 months ago)

I believe you are greatly over thinking it. You don't need that many holes nor 4'' of perlite. My terrariums have had 1'' of perlite and done the job quite well. As long as you fan  and mist them everyday you will be fine.


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Offlinezeric
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Re: how do these jars look? pf tek, brf cakes [Re: zeric]
    #23486560 - 07/28/16 05:08 PM (7 years, 6 months ago)

Btw: the single greatest thing you can do to your crop is rolling and drinking the cakes at the proper times. Now THAT makes a huge difference.


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Invisiblemupetmower
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Re: how do these jars look? pf tek, brf cakes [Re: zeric]
    #23486561 - 07/28/16 05:08 PM (7 years, 6 months ago)

no, you do want that much perlite... 4-5in. and holes on all six sides in a 2x2in grid. at least, if you want ideal conditions for your fruits.

fanning is not the same as FAE..


go take a look at the link i just posted.


EDIT - so, youre saying that proper FAE wont make that much difference? i disagree.


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


Edited by mupetmower (07/28/16 05:10 PM)


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Offlinemrmazdarx9
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Re: how do these jars look? pf tek, brf cakes [Re: zeric]
    #23486568 - 07/28/16 05:11 PM (7 years, 6 months ago)

Quote:

zeric said:
I believe you are greatly over thinking it. You don't need that many holes nor 4'' of perlite. My terrariums have had 1'' of perlite and done the job quite well. As long as you fan  and mist them everyday you will be fine.



Yeah don't listen to all the PROVEN advice on here listen to this guy with the terrible advice who also reckons you should waste a jar to that you sterilized to check for contams

It all looks OK mate the patties worked better than everyone expected I'm sure this will work well you listen and take in the CORRECT advice
Quote:

zeric said:
Btw: the single greatest thing you can do to your crop is rolling and drinking the cakes at the proper times. Now THAT makes a huge difference.



Dont drink your cakes :rofl:
The proper time not times to roll is after the dunk after birthing :thumbup:
Not roll dunk or drink


Mine make sure its off the ground


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Edited by mrmazdarx9 (07/28/16 05:20 PM)


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Offlinezeric
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Re: how do these jars look? pf tek, brf cakes [Re: mrmazdarx9]
    #23486624 - 07/28/16 05:28 PM (7 years, 6 months ago)

Haha. Very nice post indeed. Obviously it was rolling and dunking.

As for the terrariums I'm just giving my humble opinion based on the experiences I've had. The OP is already completely overwhelmed and he solely has some spreading mycelium over some jars. You really believe he need the added stress of a theoretically perfect terrarium?

Plus you're shooting yourself in the foot by disregarding a simple cake for molds and bacteria presence. Did you see the guy sterilizing the materials? Do you believe he inoculated the jars in a laboratory environment in order to assure 99.999% of sterilization?

What about when he's developed that perfect terrarium with that perfect crops? Is he going to eat all them shrooms?

Anyway I must thank you as I learned something with you in this post which will save me a lot of time: Don't bother trying to help...


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Invisibledankington
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Re: how do these jars look? pf tek, brf cakes [Re: zeric]
    #23486634 - 07/28/16 05:31 PM (7 years, 6 months ago)

I don't see what's so stressful about drilling some holes in a tote.

I'd be more stressed misting and fanning all goddamned day. Fanning isn't FAE.
Helping isn't the wrong part, it's giving 20year old tips, when we do things far more efficiently nowadays.


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Invisibleh0ldthedoor
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Re: how do these jars look? pf tek, brf cakes [Re: zeric]
    #23486635 - 07/28/16 05:31 PM (7 years, 6 months ago)

Here are pics of the jars now,



Quote:

mupetmower said:
this is all you need to know.. the exact number of holes you need is however many holes it takes to drill 14in in a 2x2in grid on all sides.

why are you planning all these crazy dimensions for the holes? just make a proper SGFC, following that tek.

the perlite wont fall through. a few pieces will when you first put it in, but then it will stop, and the rest will stay just fine.

also, i would try to get it more than 2in from the floor/table. try for at least 4-6in if possible. you could use jars or canned soup/veggies, cups, etc. and ideally, you want at least a foot on all sides from walls/obstructions.




Perfect, thank you for the resource. :thumbsup:


Quote:

mrmazdarx9 said:
Quote:

zeric said:
I believe you are greatly over thinking it. You don't need that many holes nor 4'' of perlite. My terrariums have had 1'' of perlite and done the job quite well. As long as you fan  and mist them everyday you will be fine.



Yeah don't listen to all the PROVEN advice on here listen to this guy with the terrible advice who also reckons you should waste a jar to that you sterilized to check for contams

It all looks OK mate the patties worked better than everyone expected I'm sure this will work well you listen and take in the CORRECT advice
Quote:

zeric said:
Btw: the single greatest thing you can do to your crop is rolling and drinking the cakes at the proper times. Now THAT makes a huge difference.



Dont drink your cakes :rofl:
The proper time not times to roll is after the dunk after birthing :thumbup:
Not roll dunk or drink




Thank you for the kind words! :rockon:

My plan is after it looks like the jars have colonized 100%, they'll sit another seven days. Then, the stock pot will be filled with water. After opening one jar at a time, knocking them on something to get the cake free, if necessary, the cake will be gently wiped free of the dry verm layer and gently rinsed with a small stream of fresh water before being placed in the stock pot. This process will be repeated until all cakes are rinsed and placed in the stock pot. A large dinner plate will be used to hold the cakes down in the stock pot and the sauce pot will remain covered to sit for 24hrs. After 24hrs, the cakes will be rolled in dry verm until they are uniformly coated and then moved to the SGFC.

Quote:

zeric said:
I believe you are greatly over thinking it. You don't need that many holes nor 4'' of perlite. My terrariums have had 1'' of perlite and done the job quite well. As long as you fan  and mist them everyday you will be fine.



Quote:

zeric said:
Btw: the single greatest thing you can do to your crop is rolling and drinking the cakes at the proper times. Now THAT makes a huge difference.




:underageban:


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


Edited by h0ldthedoor (07/28/16 05:45 PM)


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Offlinemrmazdarx9
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Re: how do these jars look? pf tek, brf cakes [Re: zeric]
    #23486639 - 07/28/16 05:33 PM (7 years, 6 months ago)

Quote:

zeric said:
Haha. Very nice post indeed. Obviously it was rolling and dunking.

As for the terrariums I'm just giving my humble opinion based on the experiences I've had. The OP is already completely overwhelmed and he solely has some spreading mycelium over some jars. You really believe he need the added stress of a theoretically perfect terrarium?

Plus you're shooting yourself in the foot by disregarding a simple cake for molds and bacteria presence. Did you see the guy sterilizing the materials? Do you believe he inoculated the jars in a laboratory environment in order to assure 99.999% of sterilization?

What about when he's developed that perfect terrarium with that perfect crops? Is he going to eat all them shrooms?

Anyway I must thank you as I learned something with you in this post which will save me a lot of time: Don't bother trying to help...



How is he overwhelmed?
What are you on about lab techniques and 99.999% bullshit we follow teks and advice so I know he sterilized you obviously don't follow teks or we wouldn't be having this discussion
And yes don't bother trying to help when your help is wrong do your research before telling people what to do


--------------------
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Invisibledankington
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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor]
    #23486645 - 07/28/16 05:33 PM (7 years, 6 months ago)

my man, you want to dunk them before rolling them in the verm.
Then, you want to mist after they've sat in the SGFC a few hours.


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OfflineYaMoonSun
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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor]
    #23486646 - 07/28/16 05:33 PM (7 years, 6 months ago)

The yellow spots look bacterial. How do those particular jars smell?


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Invisibledankington
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Re: how do these jars look? pf tek, brf cakes [Re: YaMoonSun]
    #23486650 - 07/28/16 05:34 PM (7 years, 6 months ago)

Quote:

YaMoonSun said:
The yellow spots look bacterial. How do those particular jars smell?




I think I see some mold in there :sad:
Do any of your jars look better?


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Offlinemrmazdarx9
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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor] * 1
    #23486673 - 07/28/16 05:40 PM (7 years, 6 months ago)

Quote:

h0ldthedoor said:
Here are pics of the jars now,



Quote:

mupetmower said:
this is all you need to know.. the exact number of holes you need is however many holes it takes to drill 14in in a 2x2in grid on all sides.

why are you planning all these crazy dimensions for the holes? just make a proper SGFC, following that tek.

the perlite wont fall through. a few pieces will when you first put it in, but then it will stop, and the rest will stay just fine.

also, i would try to get it more than 2in from the floor/table. try for at least 4-6in if possible. you could use jars or canned soup/veggies, cups, etc. and ideally, you want at least a foot on all sides from walls/obstructions.




Perfect, thank you for the resource. :thumbsup:


Quote:

mrmazdarx9 said:
Quote:

zeric said:
I believe you are greatly over thinking it. You don't need that many holes nor 4'' of perlite. My terrariums have had 1'' of perlite and done the job quite well. As long as you fan  and mist them everyday you will be fine.



Yeah don't listen to all the PROVEN advice on here listen to this guy with the terrible advice who also reckons you should waste a jar to that you sterilized to check for contams

It all looks OK mate the patties worked better than everyone expected I'm sure this will work well you listen and take in the CORRECT advice
Quote:

zeric said:
Btw: the single greatest thing you can do to your crop is rolling and drinking the cakes at the proper times. Now THAT makes a huge difference.



Dont drink your cakes :rofl:
The proper time not times to roll is after the dunk after birthing :thumbup:
Not roll dunk or drink




Thank you for the kind words! :rockon:

My plan is after it looks like the jars have colonized 100%, they'll sit another seven days. Then, the stock pot will be filled with water and some verm will be ready in a large bowl. After opening one jar at a time, knocking them on something to get the cake free, if necessary, the cake will be gently wiped free of the dry verm layer and gently rinsed with a small stream of fresh water before being gently rolled in dry verm placed and then placed in the stock pot. This process will be repeated until all cakes are rinsed, covered uniformly in verm, and placed in the stock pot. A large dinner plate will be used to hold the cakes down in the stock pot and the sauce pot will remain covered to sit for 24hrs. After 24hrs, the cakes will be moved to the SGFC.

Quote:

zeric said:
I believe you are greatly over thinking it. You don't need that many holes nor 4'' of perlite. My terrariums have had 1'' of perlite and done the job quite well. As long as you fan  and mist them everyday you will be fine.



Quote:

zeric said:
Btw: the single greatest thing you can do to your crop is rolling and drinking the cakes at the proper times. Now THAT makes a huge difference.




:underageban:



Dunk first then roll unless I read it wrong quite possible drank alot of vodka and codeine


--------------------
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Invisibleh0ldthedoor
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Re: how do these jars look? pf tek, brf cakes [Re: dankington]
    #23486686 - 07/28/16 05:42 PM (7 years, 6 months ago)

Quote:

dankington said:
my man, you want to dunk them before rolling them in the verm.
Then, you want to mist after they've sat in the SGFC a few hours.




Thanks, got a little messed up with the dog buggin' me, it can be difficult to type out what you're seeing in your head with a dog silently begging for a treat, lmao.

Quote:

YaMoonSun said:
The yellow spots look bacterial. How do those particular jars smell?




Grabbed this jar



and this jar



the second smelled a little like moldy bread, maybe? the first did not smell like anything, really.

Quote:

dankington said:
Quote:

YaMoonSun said:
The yellow spots look bacterial. How do those particular jars smell?




I think I see some mold in there :sad:
Do any of your jars look better?




If it's okay with the experience in this thread, should photos be taken of all jars for evaluation? Documenting them in sequence would be a pleasure.


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


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Invisibleh0ldthedoor
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Re: how do these jars look? pf tek, brf cakes [Re: mrmazdarx9]
    #23486691 - 07/28/16 05:43 PM (7 years, 6 months ago)

Quote:

mrmazdarx9 said:
Dunk first then roll unless I read it wrong quite possible drank alot of vodka and codeine




No you read it right, I typed it wrong. Changing it now. :super:


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


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Offlinemrmazdarx9
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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor]
    #23486707 - 07/28/16 05:47 PM (7 years, 6 months ago)

Definitely more pics it's our porn :lol:
My first jars all went in the bin my second set 6 out of 7 did well one turned green which is sat in the garage away from everything and everyone I wanna see which wins the contams or myc I won't be eating anything from it just wanna see what happens


--------------------
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Invisibleh0ldthedoor
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Re: how do these jars look? pf tek, brf cakes [Re: mrmazdarx9]
    #23486713 - 07/28/16 05:49 PM (7 years, 6 months ago)

Quote:

mrmazdarx9 said:
Definitely more pics it's our porn :lol:
My first jars all went in the bin my second set 6 out of 7 did well one turned green which is sat in the garage away from everything and everyone I wanna see which wins the contams or myc I won't be eating anything from it just wanna see what happens




Okay, will have pics soon. Same here! Turns out there's a video showing pleurotus mycelium vs trich, the results were great to watch.



Also, it was just brought to my attention that the tape on the jars is not made of the right material. It looks similar to micropore tape, but is apparently made from some sort of plastic material. Should this tape be replaced with the fabric-like 3M HealthCare tape?


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


Edited by h0ldthedoor (07/28/16 05:55 PM)


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Offlinemrmazdarx9
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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor]
    #23486728 - 07/28/16 05:53 PM (7 years, 6 months ago)

Haha that's cool but why did he add the green filter at the end was he hiding the remain contams
The yellowing in the jars you have I have read metabolite's can be the cause which ain't that much of a problem but tbh I don't know enough about contams to make a judgement or pass advice about them but do a search on yellow in Pf jars


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Re: how do these jars look? pf tek, brf cakes [Re: zeric]
    #23486733 - 07/28/16 05:55 PM (7 years, 6 months ago)

Quote:

zeric said:
Haha. Very nice post indeed. Obviously it was rolling and dunking.

As for the terrariums I'm just giving my humble opinion based on the experiences I've had. The OP is already completely overwhelmed and he solely has some spreading mycelium over some jars. You really believe he need the added stress of a theoretically perfect terrarium?

Plus you're shooting yourself in the foot by disregarding a simple cake for molds and bacteria presence. Did you see the guy sterilizing the materials? Do you believe he inoculated the jars in a laboratory environment in order to assure 99.999% of sterilization?

What about when he's developed that perfect terrarium with that perfect crops? Is he going to eat all them shrooms?

Anyway I must thank you as I learned something with you in this post which will save me a lot of time: Don't bother trying to help...




I sense some butthurt.  Welp, get over it.  You're brand new and your tilting at windmills that been grinding it out here a long time.  Good luck with that.  Don't let the door hit you on the ass.


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Re: how do these jars look? pf tek, brf cakes [Re: KenInVic]
    #23486737 - 07/28/16 05:56 PM (7 years, 6 months ago)

Quote:

KenInVic said:
Quote:

zeric said:
Haha. Very nice post indeed. Obviously it was rolling and dunking.

As for the terrariums I'm just giving my humble opinion based on the experiences I've had. The OP is already completely overwhelmed and he solely has some spreading mycelium over some jars. You really believe he need the added stress of a theoretically perfect terrarium?

Plus you're shooting yourself in the foot by disregarding a simple cake for molds and bacteria presence. Did you see the guy sterilizing the materials? Do you believe he inoculated the jars in a laboratory environment in order to assure 99.999% of sterilization?

What about when he's developed that perfect terrarium with that perfect crops? Is he going to eat all them shrooms?

Anyway I must thank you as I learned something with you in this post which will save me a lot of time: Don't bother trying to help...




I sense some butthurt.  Welp, get over it.  You're brand new and your tilting at windmills that been grinding it out here a long time.  Good luck with that.  Don't let the door hit you on the ass.



:rofl::whip:


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Re: how do these jars look? pf tek, brf cakes [Re: zeric]
    #23486746 - 07/28/16 05:58 PM (7 years, 6 months ago)

Quote:

zeric said:
Do you have a non-inoculated jar for easy mould detection?

The jars look fine tough :wink:




What would he mould it into?


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Re: how do these jars look? pf tek, brf cakes [Re: KenInVic]
    #23486823 - 07/28/16 06:17 PM (7 years, 6 months ago)

Mold on the jars? Now that would be a LOL wouldn't it?

My advices came only from my experience. Nothing else. I never stated I was the supreme mushroom grower of the universe. I believe the number of posts I have make that pretty clear. But growing twice gave me the insights into the PFtek which is what i use.

How is the guy overwhelmed? He is posting pictures of growing mycelium for Christ sake.

Hey at last some coherence in this forum. The OP makes justice to his nick.


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Re: how do these jars look? pf tek, brf cakes [Re: KenInVic]
    #23486838 - 07/28/16 06:20 PM (7 years, 6 months ago)

:lolsy:

i doubt the more yellowish spots in those jars are metabolites, like someone said, since there isnt really any myc in there to create metabolites, yet.

when i saw those more yellow spots in the very first pics you posted, i just assumed it was some of the brf/verm mix that was just slightly different color, for whatever reason. idk haha. did they look like that right after you got them out of the PC? are the yellow spots getting bigger?

you'll know son enough if its fine or not, i guess.


also, thanks for that vid. was pretty cool to see that stuff grow and devour the mold.



Also, in reply to zertic: i dont think he seems overwhelmed at all :shrug:

just seems like he is posting pics of his first jars to see if thy are alright..


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Re: how do these jars look? pf tek, brf cakes [Re: mupetmower]
    #23486848 - 07/28/16 06:22 PM (7 years, 6 months ago)

:lolsy::whathesaid:


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Re: how do these jars look? pf tek, brf cakes [Re: zeric]
    #23486885 - 07/28/16 06:27 PM (7 years, 6 months ago)

Quote:

zeric said:
Mold on the jars? Now that would be a LOL wouldn't it?

My advices came only from my experience. Nothing else. I never stated I was the supreme mushroom grower of the universe. I believe the number of posts I have make that pretty clear. But growing twice gave me the insights into the PFtek which is what i use.

How is the guy overwhelmed? He is posting pictures of growing mycelium for Christ sake.

Hey at last some coherence in this forum. The OP makes justice to his nick.



He was mocking that you used the wrong spelling of mold not saying mold on the jar
Literally every noob (bar you) posts pictures of their first grow asking for peoples opinions its not being overwhelmed its simple reassurance and further guidance

Muppetmower I figured that would be the case not enough myc for metabolites but don't know enough either way to to pass judgement myself :-)


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Re: how do these jars look? pf tek, brf cakes [Re: mrmazdarx9]
    #23486996 - 07/28/16 06:57 PM (7 years, 6 months ago)

Here are the jars, tried to get every side. Jars with areas of interest, had those areas photographed and if the rest other areas appeared "normal" (just BRF cake, no growth, no yellowing). Every photo starts in the same spot, and most rotate the same amount on the second photo, after that some are equal rotations and others only show areas of interest, as mentioned above.

Enjoy!

:fonz:



--------------------
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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor]
    #23487021 - 07/28/16 07:05 PM (7 years, 6 months ago)

okay, friend. Walk us through this. Are you flaming before each inoculation?
You sufficiently sterilized your jars?
Proper dry verm layer?

I see almost all of those have mold at the site of inoculation. If you're working cleanly, especially if you're using a SAB, then you should tell the vendor that you were unable to identify any viable spores with your microscope, because of all the mold spores.


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Re: how do these jars look? pf tek, brf cakes [Re: dankington]
    #23487092 - 07/28/16 07:23 PM (7 years, 6 months ago)

Quote:

dankington said:
okay, friend. Walk us through this. Are you flaming before each inoculation?
You sufficiently sterilized your jars?
Proper dry verm layer?

I see almost all of those have mold at the site of inoculation. If you're working cleanly, especially if you're using a SAB, then you should tell the vendor that you were unable to identify any viable spores with your microscope, because of all the mold spores.





Before inoculation, wiped the needle with ISO alcohol and then flamed with a candle. Also wiped and flamed after 6 jars. And after a needle stick. One jar got a couple cc's excess due to operator error (the plunger got dry and released under pressure).

Placed some water in a stock pot with mason jar lids, foil balls, and a layer of foil between them, and 6 jars at a time. After reaching boiling, some bowls were placed on the lid to weight it down, temp was reduced per pf tek, it was still boiling well, but not hard and fast enough to dry up all the water within a short period of time. 2 cups of water had to be added towards the end of the first 90 minutes. After that less than a cup was added on the second batch.

There is ~1/2" of dry verm on top of the BRF cake. The cakes were not pressed down hard, very gently with gloved hands just enough to get keep the verm off the dry glass. Once wiped dry, the dry verm was sprinkled on top until it was about flush with the mouth of the jar. Then the jars had their inoculation points covered with small pieces of micropore tape (plastic-like, apparently wrong type, not sure how to handle this), using ungloved hands. After waiting overnight the jars were inoc'd in a SAB, then taken out and the inoculation holes were taped over all at once.

The spores were taken manually from spore prints made of wild fruit. During the print, the caps were kept under a moistened glass on alcohol wiped foil for ~4 or 5 days. After that, they were moved to the SAB and collected with a 14Ga needle, distilled water, and a dishwasher cleaned glass measuring cup. Some other spore prints were available, but were made on paper and collected in open air with only an alcohol wiped blade.

This is all new, but an earnest effort is being made to not deviate from the tek.

:takingnotes:


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


Edited by h0ldthedoor (07/28/16 07:28 PM)


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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor]
    #23487131 - 07/28/16 07:33 PM (7 years, 6 months ago)

yeah, that's the wrong tape; but tape is really unnecessary.
Your verm layer is the real filter you're using. Do not tape up after inoculation, it's best to leave the holes open (using micropore tape is okay as an added precaution, but unnecessary).

You're basically not going to move the jars after you inoculate. You should flame until the needle's red between each jar. You put your dominant arm in one hole and out the other of your SAB holding the syrings, and pull it back in to inoculate each jar. Repeat each time. Don't wipe after flaming the needle.

Oooooohhh. You made the syringe? From a wild print? You are going to learn to use agar, friend.
BRF cakes are fine for vendor syringes that have minimal contams (but still have some), but I don't know about with wild fruit and a homemade syringe…


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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor]
    #23487153 - 07/28/16 07:41 PM (7 years, 6 months ago)

Sounds like you sure had your hands full. You need to sterilize the foil in a heat resistant container prior to taking the print. The isopropyl alcohol only sanitizes, and therefore you may have had foreign spores on the foil. Also, you probably only needed to print it for about 24 hours. Hopefully a couple of those jars work out for you, but I imagine most will go bacterial, or like the bottom one, black mold. Like the post above stated, agar is a better approach from wild spores.


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Re: how do these jars look? pf tek, brf cakes [Re: YaMoonSun]
    #23487160 - 07/28/16 07:45 PM (7 years, 6 months ago)

Quote:

dankington said:
yeah, that's the wrong tape; but tape is really unnecessary.
Your verm layer is the real filter you're using. Do not tape up after inoculation, it's best to leave the holes open (using micropore tape is okay as an added precaution, but unnecessary).

You're basically not going to move the jars after you inoculate. You should flame until the needle's red between each jar. You put your dominant arm in one hole and out the other of your SAB holding the syrings, and pull it back in to inoculate each jar. Repeat each time. Don't wipe after flaming the needle.

Oooooohhh. You made the syringe? From a wild print? You are going to learn to use agar, friend.
BRF cakes are fine for vendor syringes that have minimal contams (but still have some), but I don't know about with wild fruit and a homemade syringe…




Got it, can the wrong tape be replaced?

Leave them in the SAB, check. Thanks for the tips on needle handling :smile:

Yeah, this has been a DIY through and through. Hell, ground down two pounds of brown rice in a little coffee grinder until it was almost glowing, haha.

Agar has been brought up quite a bit, there's another spore print on foil and a fruiting patty available. Lead the way...

Quote:

YaMoonSun said:
Sounds like you sure had your hands full. You need to sterilize the foil in a heat resistant container prior to taking the print. The isopropyl alcohol only sanitizes, and therefore you may have had foreign spores on the foil. Also, you probably only needed to print it for about 24 hours. Hopefully a couple of those jars work out for you, but I imagine most will go bacterial, or like the bottom one, black mold. Like the post above stated, agar is a better approach from wild spores.




Got it, will sterilize the spore print medium beforehand going forward. Yeah, did not have the SAB built yet, figured better to leave them until they could be collected in a SAB, rather than open air. Will make sure to collect after no more than 24 hours from now on. See above :smile:


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor] * 1
    #23487185 - 07/28/16 07:56 PM (7 years, 6 months ago)

No need to change the tape now, they're basically fucked. Sorry. Just learn to do agar. Make your SAB.

https://www.shroomery.org/forums/showflat.php/Number/19208976#19208976
https://www.shroomery.org/forums/showflat.php/Number/22721954#22721954
https://www.shroomery.org/forums/showflat.php/Number/21889950#21889950

I'm sure you know an SAB is just two big ol' holes in a tote. So that's easy enough. With agar you can clean your cultures and make awesome cakes, or go to bulk... which is what you'll be doing eventually anyways.


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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor]
    #23487206 - 07/28/16 08:03 PM (7 years, 6 months ago)

I've managed to do some agar work without a pressure cooker, but it's highly advised that you consider investing in one if you plan on doing frequent sterilization.

Essentially, to start off, you may want to buy some malt extract agar powder, some glade 5oz mini rounds, some micro-pore surgical tape, some cotton swabs (plain ones without plastic or lotion ect)

Here's how to make pasty plates


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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor]
    #23487226 - 07/28/16 08:10 PM (7 years, 6 months ago)

Quote:

h0ldthedoor said:
Before inoculation, wiped the needle with ISO alcohol and then flamed with a candle. Also wiped and flamed after 6 jars. And after a needle stick. One jar got a couple cc's excess due to operator error (the plunger got dry and released under pressure). (Should have mentioned this was done in a SAB)

Placed some water in a stock pot with mason jar lids, foil balls, and a layer of foil between them, and 6 jars at a time. After reaching boiling, some bowls were placed on the lid to weight it down, temp was reduced per pf tek, it was still boiling well, but not hard and fast enough to dry up all the water within a short period of time. 2 cups of water had to be added towards the end of the first 90 minutes. After that less than a cup was added on the second batch.

There is ~1/2" of dry verm on top of the BRF cake. The cakes were not pressed down hard, very gently with gloved hands just enough to get keep the verm off the dry glass. Once wiped dry, the dry verm was sprinkled on top until it was about flush with the mouth of the jar. Then the jars had their inoculation points covered with small pieces of micropore tape (plastic-like, apparently wrong type, not sure how to handle this), using ungloved hands. After waiting overnight the jars were inoc'd in a SAB, then taken out and the inoculation holes were taped over all at once.

The spores were taken manually from spore prints made of wild fruit. During the print, the caps were kept under a moistened glass on alcohol wiped foil for ~4 or 5 days. After that, they were moved to the SAB and collected with a 14Ga needle, distilled water, and a dishwasher cleaned glass measuring cup. Some other spore prints were available, but were made on paper and collected in open air with only an alcohol wiped blade.

This is all new, but an earnest effort is being made to not deviate from the tek.

:takingnotes:




Already have the SAB and used it where instructed, so far. Thank you for the links!!

Also, none of the jars should be kept and let continue? Should they all be scrapped and reused for whatever comes next?


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


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Re: how do these jars look? pf tek, brf cakes [Re: YaMoonSun]
    #23487232 - 07/28/16 08:12 PM (7 years, 6 months ago)

Quote:

YaMoonSun said:
I've managed to do some agar work without a pressure cooker, but it's highly advised that you consider investing in one if you plan on doing frequent sterilization.

Essentially, to start off, you may want to buy some malt extract agar powder, some glade 5oz mini rounds, some micro-pore surgical tape, some cotton swabs (plain ones without plastic or lotion ect)

Here's how to make pasty plates




PC is an option, it is only 6 quarts and is electric. Pressure Cooker XL or some clone. A trip to the asian grocery store possibly sufficient? Or where can the malt extract agar powder be purchased locally?


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor]
    #23487253 - 07/28/16 08:16 PM (7 years, 6 months ago)

Quote:

h0ldthedoor said:
Quote:

YaMoonSun said:
I've managed to do some agar work without a pressure cooker, but it's highly advised that you consider investing in one if you plan on doing frequent sterilization.

Essentially, to start off, you may want to buy some malt extract agar powder, some glade 5oz mini rounds, some micro-pore surgical tape, some cotton swabs (plain ones without plastic or lotion ect)

Here's how to make pasty plates




PC is an option, it is only 6 quarts and is electric. Pressure Cooker XL or some clone. A trip to the asian grocery store possibly sufficient? Or where can the malt extract agar powder be purchased locally?




I think you can get the malt extract wherever fine brewing supplies are sold.  Agar possibly an asian grocery or maybe even the pharmacy.  There are recipes here somewhere I believe, or find them in TMC if you have a copy.


--------------------
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Edited by KenInVic (07/28/16 08:18 PM)


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Re: how do these jars look? pf tek, brf cakes [Re: KenInVic]
    #23487278 - 07/28/16 08:24 PM (7 years, 6 months ago)

agar is typically in asian markets and organic grocers, or online. Get a high percentage agar. Sometimes it'll be just too soft otherwise. I like landor trading co. myself. But there are many good brands

You can use instant mashed potatoes and karo (or any other simple sugar) as in the link I gave for easy agar.

Your malt can be purchased online or at a brewer's supply like keninvic said.


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Re: how do these jars look? pf tek, brf cakes [Re: dankington]
    #23488474 - 07/29/16 07:46 AM (7 years, 5 months ago)

i didnt realize you made the syringe from a print from those cow patties.. that's definitely your problem. if you want to grow with those prints, you basically need to use agar.

otherwise, you can get a syringe from a vendor, but those arent 100% clean either. but they are usually clean enough to where you can do a pf tek grow with minimal issues, and maybe just a tiny amount of bacteria.


but sorry dude. those cakes are toast. you got mold growing on them. =[

good luck next time :thumbup:


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Re: how do these jars look? pf tek, brf cakes [Re: mupetmower]
    #23488538 - 07/29/16 08:14 AM (7 years, 5 months ago)

Thank you everyone for all the advice and feedback, so far.

This https://www.shroomery.org/forums/showflat.php/Number/19208976#19208976 looks like the best bet for getting started with agar, ASAP. After producing a few plates to work with, (inside the SAB) a spore swab will be created from an existing spore print. After collecting the spore swab, the agar mini's would be opened (one by one) and the agar rubbed with the spore swab. The mini's would be left in the SAB until healthy mycelium appears. After healthy mycelium is growing on a significant portion of the plate (in a perfect world without contam's, going over this is all just to help get my bearings), move onto the Tiger Drop or Tiger Drop 2.0 (Tic tac toe).

On the right path so far?

After doing research mostly on pf tek, this is where things get fuzzy. Hoping to be able to use a tek that does not involve any sort of care and maintenance throughout the day. There will be no one available to perform any such tasks during the work week. Also, as mentioned above, a PC is available, though it is only a 6qt electric Pressure Cooker XL clone. Thoughts?

Also, to reiterate, all of these cakes are toast? Should the jars themselves be trashed or how could they be salvaged without spreading mold indoors?


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


Edited by h0ldthedoor (07/29/16 08:16 AM)


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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor]
    #23488565 - 07/29/16 08:25 AM (7 years, 5 months ago)

after you inoc the plates, you dont need to leve them in the SAB, since thier lid will be closed.

also, you will want to make at least one transfer from the plates you inoc before using them to inoc grains or whatever. just to ensure it's clean. and since this wil be from a wild print, you will likely need to anyways, since there will probably be contams on the plate withe the myc.

some bacteria may be hidden and meshed in with the myc, too, which is why we take the leading edge of healthy growth as the transfer as soon as you start seeing the growth(when it's like the size of a dime). that way you can get some healthy myc which shouldve grew out a little quicker than the bacteria. thats the reason for one transfer at the very least.

you dont have to toss the jars themselves. just clean them out. just take the jars outside, and dumb them out out there.

but yes, they all look toast. the only one that might bee ok is F, just because i cant clearly see any of that yellow or black spots on it, but if you decide to keep it, just keep a close eye on it. the rest all have either the yellow or black, though. it seems whatever the yellow was, was just the beginnings of the black stuff.


EDIT - on another note, nice pictures, man! those look super professional haha.


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


Edited by mupetmower (07/29/16 08:29 AM)


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Re: how do these jars look? pf tek, brf cakes [Re: mupetmower]
    #23488585 - 07/29/16 08:35 AM (7 years, 5 months ago)

Quote:

mupetmower said:
after you inoc the plates, you dont need to leve them in the SAB, since thier lid will be closed.

also, you will want to make at least one transfer from the plates you inoc before using them to inoc grains or whatever. just to ensure it's clean. and since this wil be from a wild print, you will likely need to anyways, since there will probably be contams on the plate withe the myc.

some bacteria may be hidden and meshed in with the myc, too, which is why we take the leading edge of healthy growth as the transfer as soon as you start seeing the growth(when it's like the size of a dime). that way you can get some healthy myc which shouldve grew out a little quicker than the bacteria. thats the reason for one transfer at the very least.

you dont have to toss the jars themselves. just clean them out. just take the jars outside, and dumb them out out there.




To recap

When working with homemade, wild prints
1. inoc plates
2. transfer to another plate
(repeat step 2 if time permits or contams are present)
3. inoc grains or whatever

When transferring, take sample from the edge of the myc growth (away from any contams, if present), which should be dime-sized.

Also, the jars will be taken outside, dumped out, placed in a plastic bag, brought inside and run through the dishwasher.

Sound good?

Thanks, a couple sheets of copy paper were used to make a sort of backdrop on the counter :super:


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

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Edited by h0ldthedoor (07/29/16 08:36 AM)


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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor]
    #23488589 - 07/29/16 08:37 AM (7 years, 5 months ago)

sounds good. good luck :thumbup:


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Re: how do these jars look? pf tek, brf cakes [Re: mupetmower]
    #23488755 - 07/29/16 09:46 AM (7 years, 5 months ago)

Available is powdered agar and a package with "two solid chunks" as the clerk explained over the phone, is one preferred over the other?

Also, still seeking advice on this

Quote:

After doing research mostly on pf tek, this is where things get fuzzy. Hoping to be able to use a tek that does not involve any sort of care and maintenance throughout the day. There will be no one available to perform any such tasks during the work week. Also, as mentioned above, a PC is available, though it is only a 6qt electric Pressure Cooker XL clone. Thoughts?




Mainly concerned with which tek is best for this schedule and whether or not the PC available is adequate for an agar tek? Somewhere it was mentioned that the electric PC's only get to 11psi, if that's the case can adjustments be made to account for this lack of pressure or are electric PC's wholly unsuitable for these purposes?

Again, advice and feedback is welcome,

Thank you!!

:smile:


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor]
    #23488780 - 07/29/16 09:55 AM (7 years, 5 months ago)

Quote:

h0ldthedoor said:
Available is powdered agar and a package with "two solid chunks" as the clerk explained over the phone, is one preferred over the other?

Also, still seeking advice on this

Quote:

After doing research mostly on pf tek, this is where things get fuzzy. Hoping to be able to use a tek that does not involve any sort of care and maintenance throughout the day. There will be no one available to perform any such tasks during the work week. Also, as mentioned above, a PC is available, though it is only a 6qt electric Pressure Cooker XL clone. Thoughts?




Mainly concerned with which tek is best for this schedule and whether or not the PC available is adequate for an agar tek? Somewhere it was mentioned that the electric PC's only get to 11psi, if that's the case can adjustments be made to account for this lack of pressure or are electric PC's wholly unsuitable for these purposes?

Again, advice and feedback is welcome,

Thank you!!

:smile:




It sounds like your life schedule would be better suited for something like a monotub.  My understanding is they're more maintenance free than the PF tek/SGFC operation but also, a little more technical in getting started.  The results when dialed in properly are said to exceed your wildest dreams compared to PF tek (that could just be the mushrooms talking though).


--------------------
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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor]
    #23488802 - 07/29/16 10:02 AM (7 years, 5 months ago)

Quote:

h0ldthedoor said:
Available is powdered agar and a package with "two solid chunks" as the clerk explained over the phone, is one preferred over the other?

Also, still seeking advice on this

Quote:

After doing research mostly on pf tek, this is where things get fuzzy. Hoping to be able to use a tek that does not involve any sort of care and maintenance throughout the day. There will be no one available to perform any such tasks during the work week. Also, as mentioned above, a PC is available, though it is only a 6qt electric Pressure Cooker XL clone. Thoughts?




Mainly concerned with which tek is best for this schedule and whether or not the PC available is adequate for an agar tek? Somewhere it was mentioned that the electric PC's only get to 11psi, if that's the case can adjustments be made to account for this lack of pressure or are electric PC's wholly unsuitable for these purposes?

Again, advice and feedback is welcome,

Thank you!!

:smile:




Agar is typically fine after 30min. @ 10psi. I do mine for a little more, but that should be adequate.
I haven't tried agar blocks, but I like the powdered better than the fluffy crystals, myself. The powder has really good gelling strength. You will want a real PC at some point so you can have properly prepared grains for spawn.


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Re: how do these jars look? pf tek, brf cakes [Re: dankington]
    #23488886 - 07/29/16 10:29 AM (7 years, 5 months ago)

:takingnotes:


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Re: how do these jars look? pf tek, brf cakes [Re: dankington]
    #23489036 - 07/29/16 11:29 AM (7 years, 5 months ago)

Quote:

KenInVic said:
It sounds like your life schedule would be better suited for something like a monotub.  My understanding is they're more maintenance free than the PF tek/SGFC operation but also, a little more technical in getting started.  The results when dialed in properly are said to exceed your wildest dreams compared to PF tek (that could just be the mushrooms talking though).




Sounds enticing, checking into monotubs now.

Quote:

dankington said:
Agar is typically fine after 30min. @ 10psi. I do mine for a little more, but that should be adequate.
I haven't tried agar blocks, but I like the powdered better than the fluffy crystals, myself. The powder has really good gelling strength. You will want a real PC at some point so you can have properly prepared grains for spawn.




Two types were acquired, as the purchase was made before replies had been posted.

The powdered type is (1) Telephone Brand Agar-Agar Powder 25g (Product of Thailand) and the brick type is (1) Masagana Agar-Agar White 20g (Product of the Philippines).

Glad to hear the PC will work for the time being. Will be checking around for an old-school PC at the second-hand stores, though.

(Can't stop thanking everyone that is offering help and advice, thank you for your time)


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor]
    #23495903 - 07/31/16 02:43 PM (7 years, 5 months ago)

Got the plates created yesterday using the pasty plate tek (except for using an electric PC, 45m on canning preserving) and today inoc'd the plates using this https://www.shroomery.org/forums/showflat.php/Number/20630969#20630969 method, basically swab spore print, swab agar, replace lid, let sit.

So the pasty plates are in the SAB now (where the swabbing was done) and they will remain there until signs of myc appear.

There is some condensation on the inside of the plastic tubs, is this OK?


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor]
    #23495925 - 07/31/16 02:51 PM (7 years, 5 months ago)

:super:

You can also order sterile swabs. I got mine for about $5/ 100.


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Re: how do these jars look? pf tek, brf cakes [Re: dankington] * 1
    #23495944 - 07/31/16 02:57 PM (7 years, 5 months ago)

Right, used the sterile wood cotton-tipped swabs instead of q-tips.
:gome:

Only swabbed 4 plates, figured the other 4 can be used for transfers once the first 4 colonize. Hopefully they come out half-decent..  :dawerp:


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor]
    #23495973 - 07/31/16 03:08 PM (7 years, 5 months ago)

Oh, well, you should go ahead and make a few more :awesomenod:

But good going! This is how you get started right!


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Re: how do these jars look? pf tek, brf cakes [Re: dankington]
    #23502788 - 08/02/16 06:04 PM (7 years, 5 months ago)

So all jars inevitably showed mold growth, the plates are still sitting in the SAB.

Sitting around is verm, brf, perlite, potting soil (most of a 20lb bag, a few years old), ~5lbs WBS, a dozen half pint widemouth mason jars, some pint mason jars and a model 1928 walmart tub.

The tub was going to be a SGFC for pf tek, but it remains untouched and ready to be made into a monotub, if that's the consensus.

Any advice and feedback is appreciated!!


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor]
    #23504752 - 08/03/16 08:35 AM (7 years, 5 months ago)

with agar, you can go either way. i actually did this recently and loved it: used a clean clone plate to make some blenderless LI, via Munch's tek, and used that to inoc some brf cakes, about 10mL per.

the brf cakes were not made with a verm layer, though, because i was pouring the LI on top. made some lids for it with just one 1/4in hole with SFD RTV'd to the top, via Spitball's Lids Tek. made plastic lids with SFD for most("plastic lids v2" in the lid tek) and then also made some metal lids with same 1/4 hole but with Poly instead of SFD. both worked great.

All 12 jars finished colonizing much faster than traditional pf tek with a spore syringe, and all look to have very clean growth, with minimal bacteria, since starting with a clean plate. All 12 are fruiting now.

So, if you are going to go with brf cakes, i would suggest doing this, instead of fiddling with syringes.. you can also do basically the exact same process for grain jars, too. just using a little more LI per jar, and quart jars of grain.


whatever you decide to do, good luck! you should have much better ulck since you are using agar, now.


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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Re: how do these jars look? pf tek, brf cakes [Re: mupetmower]
    #23506808 - 08/03/16 06:20 PM (7 years, 5 months ago)

Sweet, thanks for all the advice!!!

Plenty to go over and absorb :smile:

Here's the growth on one of the plates. The first three plates were inoc'd by pressing firmly on a spore print and rubbing the cotton swab across, but not hard enough to cause the spores to completely rub off the foil. On the last plate, the print was swabbed hard enough to remove some spores from the foil and the agar was rubbed hard enough to leave a few black specs on the puck. After watching them since the morning following inoculation the only growth is on the last jar. The growth is right on/under the letter A in Glad, by the way the jar is upside-down with a led light under the lid. It's not a lot, but its something! :laugh:



edit:spelling


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


Edited by h0ldthedoor (08/04/16 07:01 AM)


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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor]
    #23506833 - 08/03/16 06:25 PM (7 years, 5 months ago)

it can take up to a couple weeks for spores to germinate on agar, so be patient. with those being fresh spores it might only take a few days. next time, you dont need to see spores actually come off to know they got onto the plate. they are microscopic. and you really only need 2 to land on the plate. if youre seeing black dots rubbing off, then there are likely hundreds of thousands or more on there. but keep up the good work =]


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Re: how do these jars look? pf tek, brf cakes [Re: mupetmower]
    #23507064 - 08/03/16 07:13 PM (7 years, 5 months ago)

Indeed, you may be witnessing a bacterial pool starting up from which the mycelium will have to escape. It's happened to me a few times with a contaminated print that was donated to me.


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Re: how do these jars look? pf tek, brf cakes [Re: YaMoonSun]
    #23522774 - 08/08/16 06:35 PM (7 years, 5 months ago)

Here's the same plate today



And here are the jars



Thinking of throwing some of the jars in the dirt and watering it to see what happens....

:smokinacandycane:


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor]
    #23524501 - 08/09/16 10:24 AM (7 years, 5 months ago)

ewww you kept the jars? haha

yeah go bury those somewhere and i bet some mushrooms will pop up after a while.


--------------------
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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor]
    #23524538 - 08/09/16 10:37 AM (7 years, 5 months ago)

Quote:

h0ldthedoor said:Thinking of throwing some of the jars in the dirt and watering it to see what happens....

:smokinacandycane:




Go for it.  I buried some spent Alacabenzi in the shrubs outdoors and managed to see some mushrooms, even from the spent material.  Downside, slugs love shrooms as much as I do.  Didn't get to harvest one before they had all the young caps eaten off.


--------------------
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Re: how do these jars look? pf tek, brf cakes [Re: KenInVic]
    #23524757 - 08/09/16 12:16 PM (7 years, 5 months ago)

Quote:

mupetmower said:
ewww you kept the jars? haha

yeah go bury those somewhere and i bet some mushrooms will pop up after a while.




Yeah man! There was no other need for those jars, immediately. Figured, why not leave em sit and see what happens, y'know? Kind of glad now, hopefully the amount of mycelium across all the jars will win out over the mold once they make it in the ground! Only time will tell.. pics will be added if anything comes of the jars once their emptied and buried :smile:

Quote:

KenInVic said:
Quote:

h0ldthedoor said:Thinking of throwing some of the jars in the dirt and watering it to see what happens....

:smokinacandycane:




Go for it.  I buried some spent Alacabenzi in the shrubs outdoors and managed to see some mushrooms, even from the spent material.  Downside, slugs love shrooms as much as I do.  Didn't get to harvest one before they had all the young caps eaten off.




Damn slugs!! Bet they had an awesome time after consuming those caps, especially given their size..

:raveface:

EDIT: Here's the agar plate showing the most "activity", took a while to get these pics, even using a better camera and lens instead of the cell phone camera. Turns out trying to focus on something behind plastic, especially with condensation, can be extremely difficult. Also, the images had to be hosted off-site, efforts were made to make it appear as similar as possible to the shroomery-hosted images.



Edited by h0ldthedoor (08/09/16 05:53 PM)


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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor]
    #23534066 - 08/12/16 09:26 AM (7 years, 5 months ago)

After checking the plates this AM, the plate shown in the post above is still ahead of the rest in terms of growth.

The others are starting to show a little change now too, realizing now how mycology involves a bit of "hurry up and wait" before the real fun begins...


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


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