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Invisiblemupetmower
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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor]
    #23504752 - 08/03/16 08:35 AM (7 years, 5 months ago)

with agar, you can go either way. i actually did this recently and loved it: used a clean clone plate to make some blenderless LI, via Munch's tek, and used that to inoc some brf cakes, about 10mL per.

the brf cakes were not made with a verm layer, though, because i was pouring the LI on top. made some lids for it with just one 1/4in hole with SFD RTV'd to the top, via Spitball's Lids Tek. made plastic lids with SFD for most("plastic lids v2" in the lid tek) and then also made some metal lids with same 1/4 hole but with Poly instead of SFD. both worked great.

All 12 jars finished colonizing much faster than traditional pf tek with a spore syringe, and all look to have very clean growth, with minimal bacteria, since starting with a clean plate. All 12 are fruiting now.

So, if you are going to go with brf cakes, i would suggest doing this, instead of fiddling with syringes.. you can also do basically the exact same process for grain jars, too. just using a little more LI per jar, and quart jars of grain.


whatever you decide to do, good luck! you should have much better ulck since you are using agar, now.


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-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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Invisibleh0ldthedoor
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Re: how do these jars look? pf tek, brf cakes [Re: mupetmower]
    #23506808 - 08/03/16 06:20 PM (7 years, 5 months ago)

Sweet, thanks for all the advice!!!

Plenty to go over and absorb :smile:

Here's the growth on one of the plates. The first three plates were inoc'd by pressing firmly on a spore print and rubbing the cotton swab across, but not hard enough to cause the spores to completely rub off the foil. On the last plate, the print was swabbed hard enough to remove some spores from the foil and the agar was rubbed hard enough to leave a few black specs on the puck. After watching them since the morning following inoculation the only growth is on the last jar. The growth is right on/under the letter A in Glad, by the way the jar is upside-down with a led light under the lid. It's not a lot, but its something! :laugh:



edit:spelling


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Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


Edited by h0ldthedoor (08/04/16 07:01 AM)


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Invisiblemupetmower
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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor]
    #23506833 - 08/03/16 06:25 PM (7 years, 5 months ago)

it can take up to a couple weeks for spores to germinate on agar, so be patient. with those being fresh spores it might only take a few days. next time, you dont need to see spores actually come off to know they got onto the plate. they are microscopic. and you really only need 2 to land on the plate. if youre seeing black dots rubbing off, then there are likely hundreds of thousands or more on there. but keep up the good work =]


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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OfflineYaMoonSun
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Re: how do these jars look? pf tek, brf cakes [Re: mupetmower]
    #23507064 - 08/03/16 07:13 PM (7 years, 5 months ago)

Indeed, you may be witnessing a bacterial pool starting up from which the mycelium will have to escape. It's happened to me a few times with a contaminated print that was donated to me.


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Invisibleh0ldthedoor
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Re: how do these jars look? pf tek, brf cakes [Re: YaMoonSun]
    #23522774 - 08/08/16 06:35 PM (7 years, 5 months ago)

Here's the same plate today



And here are the jars



Thinking of throwing some of the jars in the dirt and watering it to see what happens....

:smokinacandycane:


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


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Invisiblemupetmower
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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor]
    #23524501 - 08/09/16 10:24 AM (7 years, 5 months ago)

ewww you kept the jars? haha

yeah go bury those somewhere and i bet some mushrooms will pop up after a while.


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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InvisibleKenInVic
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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor]
    #23524538 - 08/09/16 10:37 AM (7 years, 5 months ago)

Quote:

h0ldthedoor said:Thinking of throwing some of the jars in the dirt and watering it to see what happens....

:smokinacandycane:




Go for it.  I buried some spent Alacabenzi in the shrubs outdoors and managed to see some mushrooms, even from the spent material.  Downside, slugs love shrooms as much as I do.  Didn't get to harvest one before they had all the young caps eaten off.


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***My SGFC***  ***ID Mushrooms Here***
Pondering the question, "Are we all here, because we're not all there?"
       

"Because something is happening here, but you don't know what it is, do you, Mr. Jones."  Ballad of a Thin Man by Mrs. Zimmerman's little boy, Bobby.


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Invisibleh0ldthedoor
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Re: how do these jars look? pf tek, brf cakes [Re: KenInVic]
    #23524757 - 08/09/16 12:16 PM (7 years, 5 months ago)

Quote:

mupetmower said:
ewww you kept the jars? haha

yeah go bury those somewhere and i bet some mushrooms will pop up after a while.




Yeah man! There was no other need for those jars, immediately. Figured, why not leave em sit and see what happens, y'know? Kind of glad now, hopefully the amount of mycelium across all the jars will win out over the mold once they make it in the ground! Only time will tell.. pics will be added if anything comes of the jars once their emptied and buried :smile:

Quote:

KenInVic said:
Quote:

h0ldthedoor said:Thinking of throwing some of the jars in the dirt and watering it to see what happens....

:smokinacandycane:




Go for it.  I buried some spent Alacabenzi in the shrubs outdoors and managed to see some mushrooms, even from the spent material.  Downside, slugs love shrooms as much as I do.  Didn't get to harvest one before they had all the young caps eaten off.




Damn slugs!! Bet they had an awesome time after consuming those caps, especially given their size..

:raveface:

EDIT: Here's the agar plate showing the most "activity", took a while to get these pics, even using a better camera and lens instead of the cell phone camera. Turns out trying to focus on something behind plastic, especially with condensation, can be extremely difficult. Also, the images had to be hosted off-site, efforts were made to make it appear as similar as possible to the shroomery-hosted images.



Edited by h0ldthedoor (08/09/16 05:53 PM)


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Invisibleh0ldthedoor
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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor]
    #23534066 - 08/12/16 09:26 AM (7 years, 5 months ago)

After checking the plates this AM, the plate shown in the post above is still ahead of the rest in terms of growth.

The others are starting to show a little change now too, realizing now how mycology involves a bit of "hurry up and wait" before the real fun begins...


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


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