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Offlinemrmazdarx9
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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor] * 1
    #23486673 - 07/28/16 05:40 PM (7 years, 6 months ago)

Quote:

h0ldthedoor said:
Here are pics of the jars now,



Quote:

mupetmower said:
this is all you need to know.. the exact number of holes you need is however many holes it takes to drill 14in in a 2x2in grid on all sides.

why are you planning all these crazy dimensions for the holes? just make a proper SGFC, following that tek.

the perlite wont fall through. a few pieces will when you first put it in, but then it will stop, and the rest will stay just fine.

also, i would try to get it more than 2in from the floor/table. try for at least 4-6in if possible. you could use jars or canned soup/veggies, cups, etc. and ideally, you want at least a foot on all sides from walls/obstructions.




Perfect, thank you for the resource. :thumbsup:


Quote:

mrmazdarx9 said:
Quote:

zeric said:
I believe you are greatly over thinking it. You don't need that many holes nor 4'' of perlite. My terrariums have had 1'' of perlite and done the job quite well. As long as you fan  and mist them everyday you will be fine.



Yeah don't listen to all the PROVEN advice on here listen to this guy with the terrible advice who also reckons you should waste a jar to that you sterilized to check for contams

It all looks OK mate the patties worked better than everyone expected I'm sure this will work well you listen and take in the CORRECT advice
Quote:

zeric said:
Btw: the single greatest thing you can do to your crop is rolling and drinking the cakes at the proper times. Now THAT makes a huge difference.



Dont drink your cakes :rofl:
The proper time not times to roll is after the dunk after birthing :thumbup:
Not roll dunk or drink




Thank you for the kind words! :rockon:

My plan is after it looks like the jars have colonized 100%, they'll sit another seven days. Then, the stock pot will be filled with water and some verm will be ready in a large bowl. After opening one jar at a time, knocking them on something to get the cake free, if necessary, the cake will be gently wiped free of the dry verm layer and gently rinsed with a small stream of fresh water before being gently rolled in dry verm placed and then placed in the stock pot. This process will be repeated until all cakes are rinsed, covered uniformly in verm, and placed in the stock pot. A large dinner plate will be used to hold the cakes down in the stock pot and the sauce pot will remain covered to sit for 24hrs. After 24hrs, the cakes will be moved to the SGFC.

Quote:

zeric said:
I believe you are greatly over thinking it. You don't need that many holes nor 4'' of perlite. My terrariums have had 1'' of perlite and done the job quite well. As long as you fan  and mist them everyday you will be fine.



Quote:

zeric said:
Btw: the single greatest thing you can do to your crop is rolling and drinking the cakes at the proper times. Now THAT makes a huge difference.




:underageban:



Dunk first then roll unless I read it wrong quite possible drank alot of vodka and codeine


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Invisibleh0ldthedoor
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Re: how do these jars look? pf tek, brf cakes [Re: dankington]
    #23486686 - 07/28/16 05:42 PM (7 years, 6 months ago)

Quote:

dankington said:
my man, you want to dunk them before rolling them in the verm.
Then, you want to mist after they've sat in the SGFC a few hours.




Thanks, got a little messed up with the dog buggin' me, it can be difficult to type out what you're seeing in your head with a dog silently begging for a treat, lmao.

Quote:

YaMoonSun said:
The yellow spots look bacterial. How do those particular jars smell?




Grabbed this jar



and this jar



the second smelled a little like moldy bread, maybe? the first did not smell like anything, really.

Quote:

dankington said:
Quote:

YaMoonSun said:
The yellow spots look bacterial. How do those particular jars smell?




I think I see some mold in there :sad:
Do any of your jars look better?




If it's okay with the experience in this thread, should photos be taken of all jars for evaluation? Documenting them in sequence would be a pleasure.


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


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Invisibleh0ldthedoor
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Re: how do these jars look? pf tek, brf cakes [Re: mrmazdarx9]
    #23486691 - 07/28/16 05:43 PM (7 years, 6 months ago)

Quote:

mrmazdarx9 said:
Dunk first then roll unless I read it wrong quite possible drank alot of vodka and codeine




No you read it right, I typed it wrong. Changing it now. :super:


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


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Offlinemrmazdarx9
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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor]
    #23486707 - 07/28/16 05:47 PM (7 years, 6 months ago)

Definitely more pics it's our porn :lol:
My first jars all went in the bin my second set 6 out of 7 did well one turned green which is sat in the garage away from everything and everyone I wanna see which wins the contams or myc I won't be eating anything from it just wanna see what happens


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Invisibleh0ldthedoor
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Re: how do these jars look? pf tek, brf cakes [Re: mrmazdarx9]
    #23486713 - 07/28/16 05:49 PM (7 years, 6 months ago)

Quote:

mrmazdarx9 said:
Definitely more pics it's our porn :lol:
My first jars all went in the bin my second set 6 out of 7 did well one turned green which is sat in the garage away from everything and everyone I wanna see which wins the contams or myc I won't be eating anything from it just wanna see what happens




Okay, will have pics soon. Same here! Turns out there's a video showing pleurotus mycelium vs trich, the results were great to watch.



Also, it was just brought to my attention that the tape on the jars is not made of the right material. It looks similar to micropore tape, but is apparently made from some sort of plastic material. Should this tape be replaced with the fabric-like 3M HealthCare tape?


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


Edited by h0ldthedoor (07/28/16 05:55 PM)


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Offlinemrmazdarx9
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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor]
    #23486728 - 07/28/16 05:53 PM (7 years, 6 months ago)

Haha that's cool but why did he add the green filter at the end was he hiding the remain contams
The yellowing in the jars you have I have read metabolite's can be the cause which ain't that much of a problem but tbh I don't know enough about contams to make a judgement or pass advice about them but do a search on yellow in Pf jars


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InvisibleKenInVic
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Re: how do these jars look? pf tek, brf cakes [Re: zeric]
    #23486733 - 07/28/16 05:55 PM (7 years, 6 months ago)

Quote:

zeric said:
Haha. Very nice post indeed. Obviously it was rolling and dunking.

As for the terrariums I'm just giving my humble opinion based on the experiences I've had. The OP is already completely overwhelmed and he solely has some spreading mycelium over some jars. You really believe he need the added stress of a theoretically perfect terrarium?

Plus you're shooting yourself in the foot by disregarding a simple cake for molds and bacteria presence. Did you see the guy sterilizing the materials? Do you believe he inoculated the jars in a laboratory environment in order to assure 99.999% of sterilization?

What about when he's developed that perfect terrarium with that perfect crops? Is he going to eat all them shrooms?

Anyway I must thank you as I learned something with you in this post which will save me a lot of time: Don't bother trying to help...




I sense some butthurt.  Welp, get over it.  You're brand new and your tilting at windmills that been grinding it out here a long time.  Good luck with that.  Don't let the door hit you on the ass.


--------------------
***My SGFC***  ***ID Mushrooms Here***
Pondering the question, "Are we all here, because we're not all there?"
       

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Offlinemrmazdarx9
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Re: how do these jars look? pf tek, brf cakes [Re: KenInVic]
    #23486737 - 07/28/16 05:56 PM (7 years, 6 months ago)

Quote:

KenInVic said:
Quote:

zeric said:
Haha. Very nice post indeed. Obviously it was rolling and dunking.

As for the terrariums I'm just giving my humble opinion based on the experiences I've had. The OP is already completely overwhelmed and he solely has some spreading mycelium over some jars. You really believe he need the added stress of a theoretically perfect terrarium?

Plus you're shooting yourself in the foot by disregarding a simple cake for molds and bacteria presence. Did you see the guy sterilizing the materials? Do you believe he inoculated the jars in a laboratory environment in order to assure 99.999% of sterilization?

What about when he's developed that perfect terrarium with that perfect crops? Is he going to eat all them shrooms?

Anyway I must thank you as I learned something with you in this post which will save me a lot of time: Don't bother trying to help...




I sense some butthurt.  Welp, get over it.  You're brand new and your tilting at windmills that been grinding it out here a long time.  Good luck with that.  Don't let the door hit you on the ass.



:rofl::whip:


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InvisibleKenInVic
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Re: how do these jars look? pf tek, brf cakes [Re: zeric]
    #23486746 - 07/28/16 05:58 PM (7 years, 6 months ago)

Quote:

zeric said:
Do you have a non-inoculated jar for easy mould detection?

The jars look fine tough :wink:




What would he mould it into?


--------------------
***My SGFC***  ***ID Mushrooms Here***
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Offlinezeric
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Re: how do these jars look? pf tek, brf cakes [Re: KenInVic]
    #23486823 - 07/28/16 06:17 PM (7 years, 6 months ago)

Mold on the jars? Now that would be a LOL wouldn't it?

My advices came only from my experience. Nothing else. I never stated I was the supreme mushroom grower of the universe. I believe the number of posts I have make that pretty clear. But growing twice gave me the insights into the PFtek which is what i use.

How is the guy overwhelmed? He is posting pictures of growing mycelium for Christ sake.

Hey at last some coherence in this forum. The OP makes justice to his nick.


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Re: how do these jars look? pf tek, brf cakes [Re: KenInVic]
    #23486838 - 07/28/16 06:20 PM (7 years, 6 months ago)

:lolsy:

i doubt the more yellowish spots in those jars are metabolites, like someone said, since there isnt really any myc in there to create metabolites, yet.

when i saw those more yellow spots in the very first pics you posted, i just assumed it was some of the brf/verm mix that was just slightly different color, for whatever reason. idk haha. did they look like that right after you got them out of the PC? are the yellow spots getting bigger?

you'll know son enough if its fine or not, i guess.


also, thanks for that vid. was pretty cool to see that stuff grow and devour the mold.



Also, in reply to zertic: i dont think he seems overwhelmed at all :shrug:

just seems like he is posting pics of his first jars to see if thy are alright..


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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OfflineTeeRoy87
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Re: how do these jars look? pf tek, brf cakes [Re: mupetmower]
    #23486848 - 07/28/16 06:22 PM (7 years, 6 months ago)

:lolsy::whathesaid:


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Offlinemrmazdarx9
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Re: how do these jars look? pf tek, brf cakes [Re: zeric]
    #23486885 - 07/28/16 06:27 PM (7 years, 6 months ago)

Quote:

zeric said:
Mold on the jars? Now that would be a LOL wouldn't it?

My advices came only from my experience. Nothing else. I never stated I was the supreme mushroom grower of the universe. I believe the number of posts I have make that pretty clear. But growing twice gave me the insights into the PFtek which is what i use.

How is the guy overwhelmed? He is posting pictures of growing mycelium for Christ sake.

Hey at last some coherence in this forum. The OP makes justice to his nick.



He was mocking that you used the wrong spelling of mold not saying mold on the jar
Literally every noob (bar you) posts pictures of their first grow asking for peoples opinions its not being overwhelmed its simple reassurance and further guidance

Muppetmower I figured that would be the case not enough myc for metabolites but don't know enough either way to to pass judgement myself :-)


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Invisibleh0ldthedoor
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Re: how do these jars look? pf tek, brf cakes [Re: mrmazdarx9]
    #23486996 - 07/28/16 06:57 PM (7 years, 6 months ago)

Here are the jars, tried to get every side. Jars with areas of interest, had those areas photographed and if the rest other areas appeared "normal" (just BRF cake, no growth, no yellowing). Every photo starts in the same spot, and most rotate the same amount on the second photo, after that some are equal rotations and others only show areas of interest, as mentioned above.

Enjoy!

:fonz:



--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor]
    #23487021 - 07/28/16 07:05 PM (7 years, 6 months ago)

okay, friend. Walk us through this. Are you flaming before each inoculation?
You sufficiently sterilized your jars?
Proper dry verm layer?

I see almost all of those have mold at the site of inoculation. If you're working cleanly, especially if you're using a SAB, then you should tell the vendor that you were unable to identify any viable spores with your microscope, because of all the mold spores.


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Invisibleh0ldthedoor
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Re: how do these jars look? pf tek, brf cakes [Re: dankington]
    #23487092 - 07/28/16 07:23 PM (7 years, 6 months ago)

Quote:

dankington said:
okay, friend. Walk us through this. Are you flaming before each inoculation?
You sufficiently sterilized your jars?
Proper dry verm layer?

I see almost all of those have mold at the site of inoculation. If you're working cleanly, especially if you're using a SAB, then you should tell the vendor that you were unable to identify any viable spores with your microscope, because of all the mold spores.





Before inoculation, wiped the needle with ISO alcohol and then flamed with a candle. Also wiped and flamed after 6 jars. And after a needle stick. One jar got a couple cc's excess due to operator error (the plunger got dry and released under pressure).

Placed some water in a stock pot with mason jar lids, foil balls, and a layer of foil between them, and 6 jars at a time. After reaching boiling, some bowls were placed on the lid to weight it down, temp was reduced per pf tek, it was still boiling well, but not hard and fast enough to dry up all the water within a short period of time. 2 cups of water had to be added towards the end of the first 90 minutes. After that less than a cup was added on the second batch.

There is ~1/2" of dry verm on top of the BRF cake. The cakes were not pressed down hard, very gently with gloved hands just enough to get keep the verm off the dry glass. Once wiped dry, the dry verm was sprinkled on top until it was about flush with the mouth of the jar. Then the jars had their inoculation points covered with small pieces of micropore tape (plastic-like, apparently wrong type, not sure how to handle this), using ungloved hands. After waiting overnight the jars were inoc'd in a SAB, then taken out and the inoculation holes were taped over all at once.

The spores were taken manually from spore prints made of wild fruit. During the print, the caps were kept under a moistened glass on alcohol wiped foil for ~4 or 5 days. After that, they were moved to the SAB and collected with a 14Ga needle, distilled water, and a dishwasher cleaned glass measuring cup. Some other spore prints were available, but were made on paper and collected in open air with only an alcohol wiped blade.

This is all new, but an earnest effort is being made to not deviate from the tek.

:takingnotes:


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


Edited by h0ldthedoor (07/28/16 07:28 PM)


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Invisibledankington
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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor]
    #23487131 - 07/28/16 07:33 PM (7 years, 6 months ago)

yeah, that's the wrong tape; but tape is really unnecessary.
Your verm layer is the real filter you're using. Do not tape up after inoculation, it's best to leave the holes open (using micropore tape is okay as an added precaution, but unnecessary).

You're basically not going to move the jars after you inoculate. You should flame until the needle's red between each jar. You put your dominant arm in one hole and out the other of your SAB holding the syrings, and pull it back in to inoculate each jar. Repeat each time. Don't wipe after flaming the needle.

Oooooohhh. You made the syringe? From a wild print? You are going to learn to use agar, friend.
BRF cakes are fine for vendor syringes that have minimal contams (but still have some), but I don't know about with wild fruit and a homemade syringe…


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OfflineYaMoonSun
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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor]
    #23487153 - 07/28/16 07:41 PM (7 years, 6 months ago)

Sounds like you sure had your hands full. You need to sterilize the foil in a heat resistant container prior to taking the print. The isopropyl alcohol only sanitizes, and therefore you may have had foreign spores on the foil. Also, you probably only needed to print it for about 24 hours. Hopefully a couple of those jars work out for you, but I imagine most will go bacterial, or like the bottom one, black mold. Like the post above stated, agar is a better approach from wild spores.


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Invisibleh0ldthedoor
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Re: how do these jars look? pf tek, brf cakes [Re: YaMoonSun]
    #23487160 - 07/28/16 07:45 PM (7 years, 6 months ago)

Quote:

dankington said:
yeah, that's the wrong tape; but tape is really unnecessary.
Your verm layer is the real filter you're using. Do not tape up after inoculation, it's best to leave the holes open (using micropore tape is okay as an added precaution, but unnecessary).

You're basically not going to move the jars after you inoculate. You should flame until the needle's red between each jar. You put your dominant arm in one hole and out the other of your SAB holding the syrings, and pull it back in to inoculate each jar. Repeat each time. Don't wipe after flaming the needle.

Oooooohhh. You made the syringe? From a wild print? You are going to learn to use agar, friend.
BRF cakes are fine for vendor syringes that have minimal contams (but still have some), but I don't know about with wild fruit and a homemade syringe…




Got it, can the wrong tape be replaced?

Leave them in the SAB, check. Thanks for the tips on needle handling :smile:

Yeah, this has been a DIY through and through. Hell, ground down two pounds of brown rice in a little coffee grinder until it was almost glowing, haha.

Agar has been brought up quite a bit, there's another spore print on foil and a fruiting patty available. Lead the way...

Quote:

YaMoonSun said:
Sounds like you sure had your hands full. You need to sterilize the foil in a heat resistant container prior to taking the print. The isopropyl alcohol only sanitizes, and therefore you may have had foreign spores on the foil. Also, you probably only needed to print it for about 24 hours. Hopefully a couple of those jars work out for you, but I imagine most will go bacterial, or like the bottom one, black mold. Like the post above stated, agar is a better approach from wild spores.




Got it, will sterilize the spore print medium beforehand going forward. Yeah, did not have the SAB built yet, figured better to leave them until they could be collected in a SAB, rather than open air. Will make sure to collect after no more than 24 hours from now on. See above :smile:


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


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Re: how do these jars look? pf tek, brf cakes [Re: h0ldthedoor] * 1
    #23487185 - 07/28/16 07:56 PM (7 years, 6 months ago)

No need to change the tape now, they're basically fucked. Sorry. Just learn to do agar. Make your SAB.

https://www.shroomery.org/forums/showflat.php/Number/19208976#19208976
https://www.shroomery.org/forums/showflat.php/Number/22721954#22721954
https://www.shroomery.org/forums/showflat.php/Number/21889950#21889950

I'm sure you know an SAB is just two big ol' holes in a tote. So that's easy enough. With agar you can clean your cultures and make awesome cakes, or go to bulk... which is what you'll be doing eventually anyways.


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* Re: Is the PF Tek Reliable ??? LinkA 2,582 2 06/26/00 09:48 AM
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