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r35l
innoculatory engineer

Registered: 07/18/16
Posts: 34
Last seen: 1 year, 1 month
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Disposable Petri Dishes and MEA
#23482430 - 07/27/16 01:57 PM (7 years, 6 months ago) |
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Can you Sterilize disposable petri dishes in PC?
I read somewhere to be carefull how you bring the PC up to operating temp/pressure to prevent them from boiling over. what is the 'right' way?
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The Mycologist
Explorer

Registered: 05/06/16
Posts: 3,024
Last seen: 30 days, 10 hours
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Re: Disposable Petri Dishes and MEA [Re: r35l]
#23482442 - 07/27/16 02:00 PM (7 years, 6 months ago) |
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Depending on the plastic they will probably melt. They should come pre-sterilized though. Just pc a large jar with the agar in it then poor the agar into the pre-sterilized plates in a clean area.
-------------------- "That you are here—that life exists, and identity; That the powerful play goes on, and you will contribute a verse.” ― Walt Whitman, Leaves of Grass

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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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No. They're disposable. And this is not advanced.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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r35l
innoculatory engineer

Registered: 07/18/16
Posts: 34
Last seen: 1 year, 1 month
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Re: Disposable Petri Dishes and MEA [Re: 36fuckin5]
#23485525 - 07/28/16 11:55 AM (7 years, 6 months ago) |
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Quote:
36fuckin5 said: No. They're disposable. And this is not advanced.
so you're telling me agar questions go in cultivation? what noob uses agar? don't be a dick...
in fact, i know there are ways to do this i,e steam sterilizing for 90-120min... or a level three germacide for cold sterilization.... i am learning through experience and experienced both of those recently... i just wanted to know if anyone had PC'd them, would be much easier, but i see now the plastic melts. they are disposable, yes, but so is a water bottle and they get re-used all the time
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Psychedelic Pupil
Goober



Registered: 09/27/12
Posts: 744
Loc: The bright side of life
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Re: Disposable Petri Dishes and MEA [Re: r35l]
#23486340 - 07/28/16 04:03 PM (7 years, 6 months ago) |
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The short answer is no. The longer answer, really is it depends on the plastic they are made from. Most disposable petri dishes are made from Polystyrene which becomes malleable at 85C or 185F so they are not suited to pressure cooking. If you find a "disposable" petri dish made from another material that can stand up to the temps... please let everyone know. They are not sold under the label of "disposable" though.
If your looking for an easy agar solution I'd look at this from Pastywhyte
https://www.shroomery.org/forums/showflat.php/Number/19208976
-------------------- I'd like to think I'm smart enough to realize how much knowledge I don't have.
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mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
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Re: Disposable Petri Dishes and MEA [Re: r35l]
#23486374 - 07/28/16 04:11 PM (7 years, 6 months ago) |
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Quote:
r35l said:
Quote:
36fuckin5 said: No. They're disposable. And this is not advanced.
so you're telling me agar questions go in cultivation? what noob uses agar? don't be a dick...
in fact, i know there are ways to do this i,e steam sterilizing for 90-120min... or a level three germacide for cold sterilization.... i am learning through experience and experienced both of those recently... i just wanted to know if anyone had PC'd them, would be much easier, but i see now the plastic melts. they are disposable, yes, but so is a water bottle and they get re-used all the time
Noobs use agar too And it isnt a noob question your right but there isn't a retarded forum you can post this question in Disposal Petri's are one use, you could probably clean and dry toilet roll but why the fuck would you want to
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Disposable Petri Dishes and MEA [Re: r35l]
#23486593 - 07/28/16 05:20 PM (7 years, 6 months ago) |
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Quote:
r35l said:
Quote:
36fuckin5 said: No. They're disposable. And this is not advanced.
so you're telling me agar questions go in cultivation? what noob uses agar? don't be a dick...
in fact, i know there are ways to do this i,e steam sterilizing for 90-120min... or a level three germacide for cold sterilization.... i am learning through experience and experienced both of those recently... i just wanted to know if anyone had PC'd them, would be much easier, but i see now the plastic melts. they are disposable, yes, but so is a water bottle and they get re-used all the time
Yes, agar questions go in cult. If you read the description of this forum you'll see that it's supposed to be for experimental stuff for the most part.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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r35l
innoculatory engineer

Registered: 07/18/16
Posts: 34
Last seen: 1 year, 1 month
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Re: Disposable Petri Dishes and MEA [Re: 36fuckin5]
#23487168 - 07/28/16 07:49 PM (7 years, 6 months ago) |
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thanks 36troll5, for being so encouraging and such... you sound like you need to get laid... too bad theres not a dick you can grow for you to ride on... is there a forum for that? ya mealyprick....i dont need an english lesson on the meaning of disposable...
everyone else 'preciate the input, i read pasty's tek awhile ago, and theres an exception to every rule, including the 'disposable' one...as seen in pasty's tek. pretty sure those are semi disposable plastic containers too...
as for cleaning a toilet roll i think that is cyans and azurescens....retard. not where i am going with this..... clever tho, very clever. bet your mom is proud of you everytime you come up from the basement for a juice box. lol lolto bad your not a MOD, 36troll5, you would be giving the shroomery a pleasent community atmosphere and encouraging the betterment of science hobbyist everywhere.....
oh and ps...if reusing 'disposable dishes' isnt experimental idk what is YOU PREBUESCENT Fuck...go run your dicksuckers elswhere Mr. Fabulous wannabe MOD, your TC tag doesnt give you the right to bash peoples ideas and act like you invented the wheel. go fuck someone elses goat, god knows your wife is tired of it lol lol
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Disposable Petri Dishes and MEA [Re: r35l] 1
#23487505 - 07/28/16 09:43 PM (7 years, 6 months ago) |
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Nice melt bro.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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r35l
innoculatory engineer

Registered: 07/18/16
Posts: 34
Last seen: 1 year, 1 month
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Re: Disposable Petri Dishes and MEA [Re: 36fuckin5]
#23487765 - 07/28/16 10:56 PM (7 years, 6 months ago) |
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weetsie
unlicensed tub surgeon



Registered: 05/08/11
Posts: 572
Loc: United Kingdom
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Re: Disposable Petri Dishes and MEA [Re: r35l]
#23487897 - 07/29/16 12:02 AM (7 years, 6 months ago) |
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Search #1: disposable petri dish specification Search #2: polystyrene melting point
You're right disposable doesn't necessarily mean disposable, but in the time it took you to post you could of found the answer yourself.
-------------------- Active grow logs: Oysters on Straw Pellets Trade list
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r35l
innoculatory engineer

Registered: 07/18/16
Posts: 34
Last seen: 1 year, 1 month
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Re: Disposable Petri Dishes and MEA [Re: weetsie]
#23488013 - 07/29/16 01:21 AM (7 years, 6 months ago) |
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Quote:
weetsie said:

Search #1: disposable petri dish specification Search #2: polystyrene melting point
You're right disposable doesn't necessarily mean disposable, but in the time it took you to post you could of found the answer yourself.
I did search 1, 2 and a few more after that....
i found that most people do not, tho it is possible. i wasnt asking if they were meant to be re-used I was asking if any one has had success in cleaning them with a level three germacide or a steam bath (though a steam bath is beyond the 1eighty5 farenheit melting point of this specific plastic... also my plates are polypropylene... i said that in a previous post.... they can be microwavable.... didnt realise polystyrene was standard. that is junk plastic. idk why you would use those...they break easier than glass. this thread is turning into a CF you didnt even read my post..... regugitated info is not what i am asking about... i am asking for real tangible experience... has anyone cleaned them and reused them,,,,, simple question on an advanced topic. sorry if reading is over your heads, but dont assume it is over mine.... have been around here 2003, this is my second acct....the last one i lost password too in hiatus.... so yes. i have used a search funtion. i can even spell google without looking at my keyboard! :P so wtf shroomerites. when did you all become so much more 'headierthanthou" ?? in the time it took you to post a smart ass remark like this you could have read the question and realised i already said the dishes a PP plastic not styrene.... and that a level three is my goal... again looking for informed input, not a smartass remark from a regurgitator......
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weetsie
unlicensed tub surgeon



Registered: 05/08/11
Posts: 572
Loc: United Kingdom
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Re: Disposable Petri Dishes and MEA [Re: r35l]
#23488061 - 07/29/16 02:14 AM (7 years, 5 months ago) |
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Where did you say there were PP? Don't see it anywhere in this thread.
Ive never seen a PP petri dish advertised as disposable.
If they are PP, clean them, wrap them in foil and PC them alongside your agar.
-------------------- Active grow logs: Oysters on Straw Pellets Trade list
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r35l
innoculatory engineer

Registered: 07/18/16
Posts: 34
Last seen: 1 year, 1 month
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Re: Disposable Petri Dishes and MEA [Re: weetsie]
#23488078 - 07/29/16 02:25 AM (7 years, 5 months ago) |
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SHIT! now i am the asshole... my bad, i guess my link didnt copy... http://www.agarscientific.com/polypropylene-petri-dishes.html
there. these . ............
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weetsie
unlicensed tub surgeon



Registered: 05/08/11
Posts: 572
Loc: United Kingdom
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Re: Disposable Petri Dishes and MEA [Re: r35l]
#23488101 - 07/29/16 02:34 AM (7 years, 5 months ago) |
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Yeah they are definitely reusable.
It's best to sterilize the petris and agar separately then pour afterwards, people pouring then sterilizer are typically using much deeper containers.
Stack them up and wrap them tight in foil to keep them sterile when you move them from PC to your clean area.
-------------------- Active grow logs: Oysters on Straw Pellets Trade list
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mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
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Re: Disposable Petri Dishes and MEA [Re: weetsie]
#23488371 - 07/29/16 06:33 AM (7 years, 5 months ago) |
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 Dude that's why we gave you so much shit you said disposable which is the polystyrene ones PP5 stuff is what we all want defo reusable so thanks for the link too I'll be purchasing those And for reusable ones that's a banging price after 4 uses Ill be saving money £22 quid for 20 the disposable ones about £5 worth the investment imo
Edit: purchased
Edited by mrmazdarx9 (07/29/16 07:06 AM)
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r35l
innoculatory engineer

Registered: 07/18/16
Posts: 34
Last seen: 1 year, 1 month
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Re: Disposable Petri Dishes and MEA [Re: mrmazdarx9]
#23488974 - 07/29/16 11:09 AM (7 years, 5 months ago) |
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the <facepalm> is all mine, my bad. i thought i had published my reply with that link but obviously i did not.... hilarious. yep yep. those styrene dishes are lame. i had some once and cracked the bottom between my index and thumb as i was taping it! pp5 dishes are not that fragile.
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Disposable Petri Dishes and MEA [Re: r35l]
#23489121 - 07/29/16 12:07 PM (7 years, 5 months ago) |
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Polypropylene might be able to be PC'd depending on how thick they are. They'll warp if they're too thin. You also won't be able to see in them worth a fuck either way. So if you're trying to isolate you're just gonna have to make a guess and then open the plate for longer to make sure you got your wedge.
But this still isn't advanced. It fits in with the other threads here but they don't belong either.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
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Re: Disposable Petri Dishes and MEA [Re: 36fuckin5]
#23489156 - 07/29/16 12:20 PM (7 years, 5 months ago) |
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They look pretty translucent on the pics but I've ordered some so will report on their PCability and the viability once I get them
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
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leschampignons
Biochemistry + Mycology



Registered: 08/30/13
Posts: 1,583
Loc: NY/NJ/ME
Last seen: 4 days, 10 hours
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Re: Disposable Petri Dishes and MEA [Re: mrmazdarx9]
#23489513 - 07/29/16 02:45 PM (7 years, 5 months ago) |
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yes please keep us updated on this. Those pictures look like polystyrene because the plates are crystal clear. Once I purchased electrical tape and it came in a circular little pp5 container that looked very much like a petri dish. They weren't very thick but were still a translucent whitish clear - and I think that is just how pure polypropylene is. I PC'd them with no problem but not being able to see inside them well enough was more trouble than it was worth so I stopped using them.
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