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mrmazdarx9
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Registered: 05/15/16
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Gentlemen I invite you to start your flamethrowers first terrible agar
#23474691 - 07/25/16 07:53 AM (7 years, 6 months ago) |
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Before you see the pics let me explain all my bits and Bob's arrived for agar and I wanted a play just so I could understand the general process of how it all works I did sterilize everything PC and flame All done in SAB The plates are already in the bin
Oh god I don't wanna do this but... Have a laugh on me 
 So in the first pic I fudged up with the spores they sort of flicked off the inoculation loop all over the place
The second pic I figured maybe you gently rub it on but I'm a tad heavy handed it seems
I didn't wrap either with tape or cling film (should have read more)
Had a fairly large amount of condensation in the dishes
And I left them in the SAB in the extremely hot conservatory which explain the condensation
I have one now that I approached a lot better but that's not ready yet any one wanna place bets on how that ones gonna look
Edited by mrmazdarx9 (07/25/16 07:54 AM)
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: mrmazdarx9]
#23474716 - 07/25/16 08:12 AM (7 years, 6 months ago) |
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Wait for the bottle to cool before you pour, this will take care of the condensation. The bottle needs to be cool enough for you to handle with nothing protecting your hands.
Those are fucked, through and through, have you left a bunch of poured plates uninoculated to see if your pour was OK?
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Kush_Zombie
smug piece of shit



Registered: 10/22/14
Posts: 4,793
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: mrmazdarx9]
#23474720 - 07/25/16 08:14 AM (7 years, 6 months ago) |
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How did you distribute the spores onto the agar? sterile cotton swab?
You might have better luck with PastyPlates
-------------------- How to get started in bulk: Presto 23-Quart Pressure Cooker BOD's Simple as FUCK Still Air Box PastyWhyte's Easy Agar Tek Munchauzen's Cultivation Video Series How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs) What is G2G? (Grain-to-Grain) Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary) Spitball's Monotub Tek (A liner isn't necessary but is useful) Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.
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mrmazdarx9
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: Kush_Zombie]
#23474732 - 07/25/16 08:21 AM (7 years, 6 months ago) |
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Quote:
Supalemonhaze said: Wait for the bottle to cool before you pour, thprocessill take care of the condensation. The bottle needs to be cool enough for you to handle with nothing protecting your hands.
Those are fucked, through and through, have you left a bunch of poured plates uninoculated to see if your pour was OK?
Ive got two more in the fridge I only did a few to sort of get my head around the whole process
Quote:
Kush_Zombie said: How did you distribute the spores onto the agar? sterile cotton swab?
You might have better luck with PastyPlates
first plate I scraped a bit of the print off the foil with innoc loop so they fell on to the plate second plate I got a bit of print on the loop and tried to rub it onto the plate
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Kush_Zombie
smug piece of shit



Registered: 10/22/14
Posts: 4,793
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: mrmazdarx9]
#23474739 - 07/25/16 08:23 AM (7 years, 6 months ago) |
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is the innoc loop something that you sterilized by any chance?
-------------------- How to get started in bulk: Presto 23-Quart Pressure Cooker BOD's Simple as FUCK Still Air Box PastyWhyte's Easy Agar Tek Munchauzen's Cultivation Video Series How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs) What is G2G? (Grain-to-Grain) Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary) Spitball's Monotub Tek (A liner isn't necessary but is useful) Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.
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mrmazdarx9
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: Kush_Zombie]
#23474754 - 07/25/16 08:30 AM (7 years, 6 months ago) |
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Flame sterilised it till it glew red
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lvnthalife
Stranger


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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: mrmazdarx9]
#23474797 - 07/25/16 08:48 AM (7 years, 6 months ago) |
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Putting your petri dishes in stacks of three also helps with condensation if you have to pour your agar before it cooled.
I know im sometimes pressed for time and that was tip given to me by another member.
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Bobabouy
Shrimp Boat Captain



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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: lvnthalife]
#23474823 - 07/25/16 09:00 AM (7 years, 6 months ago) |
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Glew red.
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mrmazdarx9
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: Bobabouy]
#23474846 - 07/25/16 09:10 AM (7 years, 6 months ago) |
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Quote:
Bobabouy said: Glew red. 
Lol trust me im english haha glowed red when I typed it originally it didnt look right neither did glew but I rolled the dice and got it wrong
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Kush_Zombie
smug piece of shit



Registered: 10/22/14
Posts: 4,793
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: Bobabouy]
#23474848 - 07/25/16 09:10 AM (7 years, 6 months ago) |
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Quote:
Bobabouy said: Glew red. 
I'm glad I'm not the only one who appreciated the word "glew" I had to type it in and see if autocorrect recognized it as a word when he first posted that  It should be.
-------------------- How to get started in bulk: Presto 23-Quart Pressure Cooker BOD's Simple as FUCK Still Air Box PastyWhyte's Easy Agar Tek Munchauzen's Cultivation Video Series How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs) What is G2G? (Grain-to-Grain) Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary) Spitball's Monotub Tek (A liner isn't necessary but is useful) Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: Kush_Zombie] 1
#23474972 - 07/25/16 09:54 AM (7 years, 6 months ago) |
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You have a lot of satellites there. Need to work on your movements. Never reach over the media, be sure your box is wet, use less spores. The tiniest speck visible is like 10 million spores. Technically you only need 2.
Practice makes perfect. If you are pouring plates be sure to leave a few blanks to make sure your pour techniques are good. Later you can try a dry run with em to be sure your movements are good. Flame the tools, open the plates, touch the loop to the agar, do everything you normally would except put spores on the loop. If you can do that and have nothing show up your technique is solid.
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mrmazdarx9
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: Pastywhyte]
#23474983 - 07/25/16 09:59 AM (7 years, 6 months ago) |
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Ok yeah my skill leave alot to be desired at the mo I have a couple blanks in the fridge is it better to leave them out so I can see if any contams grow without spores on there then? with flaming the loop how long does it need to cool before use? after doing the pour I can see the benefits in the no pour
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Kush_Zombie
smug piece of shit



Registered: 10/22/14
Posts: 4,793
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: mrmazdarx9]
#23474991 - 07/25/16 10:01 AM (7 years, 6 months ago) |
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Take em out for a week or so and then throw them back in the fridge if you don't see anything wrong
-------------------- How to get started in bulk: Presto 23-Quart Pressure Cooker BOD's Simple as FUCK Still Air Box PastyWhyte's Easy Agar Tek Munchauzen's Cultivation Video Series How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs) What is G2G? (Grain-to-Grain) Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary) Spitball's Monotub Tek (A liner isn't necessary but is useful) Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.
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mrmazdarx9
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: Kush_Zombie]
#23474996 - 07/25/16 10:03 AM (7 years, 6 months ago) |
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Right ill do that now, just leave in a cupboard or in sab
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Kush_Zombie
smug piece of shit



Registered: 10/22/14
Posts: 4,793
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: mrmazdarx9]
#23475001 - 07/25/16 10:05 AM (7 years, 6 months ago) |
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I'm not familiar with pouring agar so it could be different from how I normally do it, but if the plates are sealed to where no foreign bacteria can enter the petri dish then I'd just leave them on some random shelf or something
-------------------- How to get started in bulk: Presto 23-Quart Pressure Cooker BOD's Simple as FUCK Still Air Box PastyWhyte's Easy Agar Tek Munchauzen's Cultivation Video Series How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs) What is G2G? (Grain-to-Grain) Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary) Spitball's Monotub Tek (A liner isn't necessary but is useful) Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.
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mrmazdarx9
Pffffttt


Registered: 05/15/16
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: Kush_Zombie]
#23475006 - 07/25/16 10:07 AM (7 years, 6 months ago) |
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Yeah sellotaped and clingfilmed around the circumference
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: mrmazdarx9]
#23475016 - 07/25/16 10:10 AM (7 years, 6 months ago) |
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Just leave em out. Only time I would put blank plates in the fridge is if I was trying to dry out some condensation. Agar is fine at room temp.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: Kush_Zombie]
#23475027 - 07/25/16 10:12 AM (7 years, 6 months ago) |
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Yeah, don't refrigerate blank plates. You want contams to grow if you have them so you can throw it out. Pouring is quite easy, you should do fine if you move slowly enough. Try doing a pour and leave a bunch of plates unused to see if your technique is good enough. If after a week nothing pops up, do what pasty said. Blank transfers are great for seeing where the faults lie.
When pouring agar, try to stack them as high as you can, without them getting too close to the top of the SAB ofc. The stack will prevent the ones on the bottom from clouding up with condensation. Someone above suggested stacks of 3, you should do bigger stacks. I notice that my first 3 in a stack all have at least some condensation, the one on top having the most. The ones below the 3rd will be crystal clear.
goodluck with it, keep us posted. I like seeing folks pour their own agar, people tend to think it's a lot harder than it actually is.
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Kush_Zombie
smug piece of shit



Registered: 10/22/14
Posts: 4,793
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: Supalemonhaze]
#23475045 - 07/25/16 10:18 AM (7 years, 6 months ago) |
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If he was keeping it for longer than a month he'd want it put in the fridge though, right?
-------------------- How to get started in bulk: Presto 23-Quart Pressure Cooker BOD's Simple as FUCK Still Air Box PastyWhyte's Easy Agar Tek Munchauzen's Cultivation Video Series How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs) What is G2G? (Grain-to-Grain) Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary) Spitball's Monotub Tek (A liner isn't necessary but is useful) Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: Kush_Zombie]
#23475048 - 07/25/16 10:19 AM (7 years, 6 months ago) |
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Not really.
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mrmazdarx9
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: Supalemonhaze]
#23475051 - 07/25/16 10:19 AM (7 years, 6 months ago) |
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I only did 5 but I have about 40 plates to use up so ill do that next time I get a few hours spare cheers everyone
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
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Kush_Zombie
smug piece of shit



Registered: 10/22/14
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: Supalemonhaze]
#23475054 - 07/25/16 10:20 AM (7 years, 6 months ago) |
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Oh okay. That's the only reason I said to put them back in the fridge is because I thought I remembered reading that somewhere. I'm probably getting it confused with something else.
-------------------- How to get started in bulk: Presto 23-Quart Pressure Cooker BOD's Simple as FUCK Still Air Box PastyWhyte's Easy Agar Tek Munchauzen's Cultivation Video Series How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs) What is G2G? (Grain-to-Grain) Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary) Spitball's Monotub Tek (A liner isn't necessary but is useful) Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: Kush_Zombie]
#23475059 - 07/25/16 10:21 AM (7 years, 6 months ago) |
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You put it in the fridge when you have a culture growing on it that you want to keep for a bit.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: Kush_Zombie]
#23475073 - 07/25/16 10:24 AM (7 years, 6 months ago) |
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Quote:
mrmazdarx9 said: I only did 5 but I have about 40 plates to use up so ill do that next time I get a few hours spare cheers everyone
What kind of plates are you using? Plastic? Ideally, you want to pour the whole sleeve once you open it. Once poured, let them cool and slip them back in the sleeve they came in. They will stay clean in there even though they are not individually wrapped. I left plates in that sleeve for a couple of months before I saw them growing mold. IIRC, only a couple from the top contaminated, the others were fine.
Quote:
Kush_Zombie said: Oh okay. That's the only reason I said to put them back in the fridge is because I thought I remembered reading that somewhere. I'm probably getting it confused with something else.
It takes a very long time for a plate to dry up, which apart from contams, is the only thing that will fuck it up. As I said above, I've left plates for two months unwrapped, inside the sleeve they came with and they were nowhere near drying.
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Kush_Zombie
smug piece of shit



Registered: 10/22/14
Posts: 4,793
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: Pastywhyte]
#23475078 - 07/25/16 10:26 AM (7 years, 6 months ago) |
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Yeah, I knew that but I thought it was with blanks too for some reason maybe it's the weed.
-------------------- How to get started in bulk: Presto 23-Quart Pressure Cooker BOD's Simple as FUCK Still Air Box PastyWhyte's Easy Agar Tek Munchauzen's Cultivation Video Series How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs) What is G2G? (Grain-to-Grain) Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary) Spitball's Monotub Tek (A liner isn't necessary but is useful) Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.
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mrmazdarx9
Pffffttt


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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: Supalemonhaze]
#23475082 - 07/25/16 10:26 AM (7 years, 6 months ago) |
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Yeah the 20 plate sleaves plastic ones I resealed the bag again after popping what I wanted out
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: mrmazdarx9]
#23475090 - 07/25/16 10:29 AM (7 years, 6 months ago) |
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It's best that you don't do that. Once you open a sleeve, pour it all and store it where you won't disturb it. Taking the plates in and out to pour and store everytime is bound to contam the plates before you even pour them. 20plates run out pretty quickly anyway, you'd be surprised how fast you go throught them, especially if you keep getting contams.
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: Supalemonhaze]
#23475559 - 07/25/16 01:41 PM (7 years, 6 months ago) |
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Expecting updates
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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mrmazdarx9
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: ComebackKid]
#23475586 - 07/25/16 01:49 PM (7 years, 6 months ago) |
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I have one left that I use better sterile techniques so in a few day I should have something to display whether is be a pile of crap or not
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mrmazdarx9
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: mrmazdarx9]
#23489102 - 07/29/16 11:58 AM (7 years, 5 months ago) |
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Ive bought some PP5 petridishes would these be OK to put PDA in then sterilize ala no pour tek?
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
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mrmazdarx9
Pffffttt


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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: mrmazdarx9]
#23515327 - 08/06/16 08:17 AM (7 years, 5 months ago) |
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Just inoculated now
 latest attempt both plates where left out for a little over a week and showed no sign of contams inoculated both with spores dead centre on the plates using innoc loop flamed red all done in SAB sprayed with soapy weakly bleachy water wore gloves cleaned with iso wrapped with cling film hopefully better results this time handily plate one has one spot plate two has two spots completely accidental but helpful to remember which is which for now
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: mrmazdarx9]
#23517600 - 08/07/16 01:21 AM (7 years, 5 months ago) |
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If you mean that it's a regular petri dish, then no, you cannot do a no-pour in them. PP5 petri dishes are not worth it IMO, they are cloudy and expensive. Better off using regular petri dishes or any other pp5 container.
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