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mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: Supalemonhaze]
#23475051 - 07/25/16 10:19 AM (7 years, 6 months ago) |
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I only did 5 but I have about 40 plates to use up so ill do that next time I get a few hours spare cheers everyone
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Kush_Zombie
smug piece of shit



Registered: 10/22/14
Posts: 4,793
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: Supalemonhaze]
#23475054 - 07/25/16 10:20 AM (7 years, 6 months ago) |
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Oh okay. That's the only reason I said to put them back in the fridge is because I thought I remembered reading that somewhere. I'm probably getting it confused with something else.
-------------------- How to get started in bulk: Presto 23-Quart Pressure Cooker BOD's Simple as FUCK Still Air Box PastyWhyte's Easy Agar Tek Munchauzen's Cultivation Video Series How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs) What is G2G? (Grain-to-Grain) Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary) Spitball's Monotub Tek (A liner isn't necessary but is useful) Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: Kush_Zombie]
#23475059 - 07/25/16 10:21 AM (7 years, 6 months ago) |
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You put it in the fridge when you have a culture growing on it that you want to keep for a bit.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: Kush_Zombie]
#23475073 - 07/25/16 10:24 AM (7 years, 6 months ago) |
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Quote:
mrmazdarx9 said: I only did 5 but I have about 40 plates to use up so ill do that next time I get a few hours spare cheers everyone
What kind of plates are you using? Plastic? Ideally, you want to pour the whole sleeve once you open it. Once poured, let them cool and slip them back in the sleeve they came in. They will stay clean in there even though they are not individually wrapped. I left plates in that sleeve for a couple of months before I saw them growing mold. IIRC, only a couple from the top contaminated, the others were fine.
Quote:
Kush_Zombie said: Oh okay. That's the only reason I said to put them back in the fridge is because I thought I remembered reading that somewhere. I'm probably getting it confused with something else.
It takes a very long time for a plate to dry up, which apart from contams, is the only thing that will fuck it up. As I said above, I've left plates for two months unwrapped, inside the sleeve they came with and they were nowhere near drying.
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Kush_Zombie
smug piece of shit



Registered: 10/22/14
Posts: 4,793
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: Pastywhyte]
#23475078 - 07/25/16 10:26 AM (7 years, 6 months ago) |
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Yeah, I knew that but I thought it was with blanks too for some reason maybe it's the weed.
-------------------- How to get started in bulk: Presto 23-Quart Pressure Cooker BOD's Simple as FUCK Still Air Box PastyWhyte's Easy Agar Tek Munchauzen's Cultivation Video Series How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs) What is G2G? (Grain-to-Grain) Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary) Spitball's Monotub Tek (A liner isn't necessary but is useful) Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.
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mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: Supalemonhaze]
#23475082 - 07/25/16 10:26 AM (7 years, 6 months ago) |
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Yeah the 20 plate sleaves plastic ones I resealed the bag again after popping what I wanted out
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: mrmazdarx9]
#23475090 - 07/25/16 10:29 AM (7 years, 6 months ago) |
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It's best that you don't do that. Once you open a sleeve, pour it all and store it where you won't disturb it. Taking the plates in and out to pour and store everytime is bound to contam the plates before you even pour them. 20plates run out pretty quickly anyway, you'd be surprised how fast you go throught them, especially if you keep getting contams.
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: Supalemonhaze]
#23475559 - 07/25/16 01:41 PM (7 years, 6 months ago) |
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Expecting updates
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: ComebackKid]
#23475586 - 07/25/16 01:49 PM (7 years, 6 months ago) |
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I have one left that I use better sterile techniques so in a few day I should have something to display whether is be a pile of crap or not
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
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mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: mrmazdarx9]
#23489102 - 07/29/16 11:58 AM (7 years, 5 months ago) |
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Ive bought some PP5 petridishes would these be OK to put PDA in then sterilize ala no pour tek?
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
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mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: mrmazdarx9]
#23515327 - 08/06/16 08:17 AM (7 years, 5 months ago) |
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Just inoculated now
 latest attempt both plates where left out for a little over a week and showed no sign of contams inoculated both with spores dead centre on the plates using innoc loop flamed red all done in SAB sprayed with soapy weakly bleachy water wore gloves cleaned with iso wrapped with cling film hopefully better results this time handily plate one has one spot plate two has two spots completely accidental but helpful to remember which is which for now
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Gentlemen I invite you to start your flamethrowers first terrible agar [Re: mrmazdarx9]
#23517600 - 08/07/16 01:21 AM (7 years, 5 months ago) |
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If you mean that it's a regular petri dish, then no, you cannot do a no-pour in them. PP5 petri dishes are not worth it IMO, they are cloudy and expensive. Better off using regular petri dishes or any other pp5 container.
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