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Frank_S
Stranger
Registered: 01/07/16
Posts: 4
Last seen: 6 years, 8 months
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bulk disasters.
#23474472 - 07/25/16 05:31 AM (7 years, 6 months ago) |
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Hello all. I have been doing brf cakes for about a year. With no abnormal issues. Nothing more than to be expected from a new guy. I am getting tired of making them tho and have been trying to do bulk. Alot. I am using a recipe and original spore print from a local grower who befriended me and has been helping me out soon the way. I went through 50 lbs of grain trying bulk. Everything turns green in colonization. My substrate is pasteurized. I try to get the center of the Sub and the water in the PC to stay at 165 for 90 min. Grain gets a 12 you soak with a tsp of gypsum and I heat it to almost a boil at the end of the 12 hours to help it dry. I am currently using Polly fill in the jar lids but I am getting the supplies to make plastic lids with injection port and filters on the way now. I have tried LC that I inoculated brf jars with at the same time the spawn looked good but sub went bad. They don't go bad a little. I think if was an outside contamination it would start at one spot. This looks like fluffy mycelium just a bit off white and a few hours later green mold spots everywhere. Makes me think it is contaminated spawn? I know the BRF ca
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: bulk disasters. [Re: Frank_S]
#23474494 - 07/25/16 05:47 AM (7 years, 6 months ago) |
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LC's should really ever be inoculated with agar, you'll be rolling a dice with anything else.
As for your bulk contaminating, what is your inoculation method? Are you using spores right off the print into the jar?
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Frank_S
Stranger
Registered: 01/07/16
Posts: 4
Last seen: 6 years, 8 months
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Yes I am using a SAB to inoculate in. I am taking the print and scraping into a shot glass of sterilized water. Then into the jars. New syringe and needle with flame in-between. I understand agar is the way to go. It was more of a learning experience thing. I am saving for a flow hood then I plan on trying agar.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: bulk disasters. [Re: Frank_S]
#23474561 - 07/25/16 06:33 AM (7 years, 6 months ago) |
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You can still do agar in a SAB, slightly different technique but still the same results. If your sterile tek is on point, the most likely vector I see is your print. You said a friend gave it to you, right? Home made prints are often dirtier than vendor syringes/prints. If I was in your shoes, I would give agar a go as that is the best chance you have to find out where it's going wrong. Short of agar, I would at least get vendor syringes if I wanted to go spore to grain.
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Frank_S
Stranger
Registered: 01/07/16
Posts: 4
Last seen: 6 years, 8 months
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He gave me the original print. I have taken many since. I use caps about 1-1.5 inch I found they drop alot of spores at that size and are easy to work with. I use a freshly sanitized shotgun chamber with fresh pearlight and take the cakes that look the best. I take them from the first flush. I wipe down the foil with rubbing alcohol and put them into a cleaned plastic box with a plastic cup that has been wiped with ISO over top. Wrapped in foil that has been wiped with ISO. I wear fresh gloves wipe my arms with ISO and a face mask for pretty much everything.
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Hugh Jorgan
Mycophile



Registered: 09/21/15
Posts: 173
Last seen: 2 years, 9 months
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Re: bulk disasters. [Re: Frank_S]
#23474646 - 07/25/16 07:28 AM (7 years, 6 months ago) |
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Cakes tend to me more resistant to contams than bulk. It probably is your spawn from contaminated spores. Learn to use Agar (its mind numbingly easy). You will be able to eliminate the spores as a contamination vector and may solve your problems in 1 easy step. Its better to see contams pop up on agar than to waste pound after pound of grain. I know I've been in your shoes. Once you get your sterile technique down you will be thrilled by the results.
1000G mono harvested yesterday
-------------------- Always go forward! Never go straight!!
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: bulk disasters. [Re: Frank_S]
#23474655 - 07/25/16 07:32 AM (7 years, 6 months ago) |
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Doesn't really matter, as soon as the mushroom breaks it's veil it's exposed to open air. Even while printing, it's still easy to contaminate a print. Takes a lot of practice to get something like that done good enough to use as an inoculant. Keep in mind that vendors often have positive pressure growrooms with filtered air and good enough tools to keep their prints and syringes clean, yet even those are contaminated sometimes. Gives you an idea how hard it is to get a very clean print, let alone a 100% clean one.
I only see 2 ways out of this current dilemma, either start working with agar or buy vendor syringes. Agar will be the best choice. You will save money and working with it is the best experience you can get in terms of sterile tek. Using blank agar you can easily see if your sterile tek is adequate or if you need to improve. No pour agar is ridiculously cheap to set up, and easy to do. You should give it a go.
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