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Invisiblewhere_did_Panda_go
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Making a liquid culture with a mushroom cap
    #23474393 - 07/25/16 03:40 AM (7 years, 6 months ago)

Hi,

Newbie here, I started from a growkit and decided that it could be cheaper to grow my own mushrooms by doing everything myself. So I let some mushrooms mature and decided to make some spore prints and a liquid culture.
For the liquid culture it sounded a bit tedious to first start with spore print, then scrape some of with a sterilized scraper, whatever it is called and drop it into the liquid culture or syringe it first (If I haven't screwed up by contaminating my spore print that is). So I spotted a cute glass of bottle, put some water and little honey in it and let it pressure cook for 1.30 hour. After cooling down, I decided to change the bottle cap to a mushroom cap. After 3 hours it looked like this.



I will keep the mushroom cap on for 12 hours and will use it in the coming days to drown my prepared substrate with it.  Ok not really drown, but to use alot of inucolation holes.


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InvisibleInocuole
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Re: Making a liquid culture with a mushroom cap [Re: where_did_Panda_go]
    #23474400 - 07/25/16 03:43 AM (7 years, 6 months ago)

Oh goodness no that is absolutely not how you make an LC, that is already ruined.

1. Inoculating LC with spores is reckless because spores aren't sterile and it's sugar water we're talking about here.
2. If you were to inoculate anything with spores, you'd use like, a tiny speck, you'd absolutely never let a whole cap sporulate into your medium, and especially not in.....
3. Open air
4. Drown.. the substrate?  I'm pretty sure you're trolling now.

Here.. so you're not like "But that's not helpful"

http://www.shroomery.org/forums/showflat.php/Number/19140341#19140341
https://www.shroomery.org/forums/showflat.php/Number/22721954
https://www.shroomery.org/forums/showflat.php/Number/21448273

Don't mess with LC until you've mastered agar.  Very important intermediate step there.


Edited by Inocuole (07/25/16 05:15 AM)


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Invisiblewhere_did_Panda_go
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Re: Making a liquid culture with a mushroom cap [Re: Inocuole]
    #23474543 - 07/25/16 06:16 AM (7 years, 6 months ago)

If spores are never sterile, how would it be able to not contaminate on agar? I read somewhere in recent posts agar gives spores more favorable environment, but do I really also need to add sugar and starch to an agar mixture used for desserts? Sugar no problem, but do I need to boil a potato to get starch?

I assumed that the mushroom cap is covering my bottle, so only spores can drop into the liquid culture. Or do you think this might had a better chance if I only used sterilized water? Most spore syringe only contain water and spores, would that not be a problem with contamination too?


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OfflineYerow
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Re: Making a liquid culture with a mushroom cap [Re: where_did_Panda_go]
    #23474583 - 07/25/16 06:46 AM (7 years, 6 months ago)

Oh my. The cap itself is covered in bacteria and other sorts of shit that's dropping down into the bottle, + the mushroom is nowhere near enough to prevent shit from the air getting down there..

We use agar to start cultures so we can transfer away from contams, and then use the clean agar for LC or whatever


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InvisibleSupalemonhaze
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Re: Making a liquid culture with a mushroom cap [Re: Yerow]
    #23474670 - 07/25/16 07:42 AM (7 years, 6 months ago)

That thing is uber fucked. Possibly the funniest shit I've seen so far. :lolsy:


OP, you really need to do a lot of reading. But to answer your question, agar still contaminates but since it's a 2d surface, you can take a piece of clean mycelium and transfer that into another plate until you get a clean culture. You then proceed to cut the plate into wedges and drop them into grain jars. If you wanna do liquids, I suggest LI over LC for a begginer, it's easier and most definitely safer.

Even I have trouble keeping an LC clean and I can do agar pretty easily and with minimal contamination. Using a mushroom cap as a lid in an open air environment is about the worst thing you can do, not to mention the funniest. Trash that thing before it gives you aids.


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Invisiblewhere_did_Panda_go
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Re: Making a liquid culture with a mushroom cap [Re: Supalemonhaze]
    #23474806 - 07/25/16 08:53 AM (7 years, 6 months ago)

Nah I want to see what happens, the world needs more love and aids anyway. Plus I can't throw it away, it's staring at me not to do it...



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Invisiblemupetmower
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Re: Making a liquid culture with a mushroom cap [Re: where_did_Panda_go]
    #23474811 - 07/25/16 08:55 AM (7 years, 6 months ago)

this just made my day :lol:


--------------------
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OfflineBobabouy
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Re: Making a liquid culture with a mushroom cap [Re: where_did_Panda_go]
    #23474816 - 07/25/16 08:57 AM (7 years, 6 months ago)

Can never have enough aids. As long as it's in Africa.


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Invisibler.lutece
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Re: Making a liquid culture with a mushroom cap [Re: Bobabouy]
    #23474827 - 07/25/16 09:00 AM (7 years, 6 months ago)

God, it literally has a face.


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InvisibleMunchauzen
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Re: Making a liquid culture with a mushroom cap [Re: where_did_Panda_go]
    #23474833 - 07/25/16 09:02 AM (7 years, 6 months ago)

Quote:

where_did_Panda_go said:
If spores are never sterile, how would it be able to not contaminate on agar? I read somewhere in recent posts agar gives spores more favorable environment, but do I really also need to add sugar and starch to an agar mixture used for desserts? Sugar no problem, but do I need to boil a potato to get starch?

I assumed that the mushroom cap is covering my bottle, so only spores can drop into the liquid culture. Or do you think this might had a better chance if I only used sterilized water? Most spore syringe only contain water and spores, would that not be a problem with contamination too?



On agar we can transfer away from contaminates to clean our culture up. A few generations of transfers and ur culture is squeaky clean.


Edited by Munchauzen (07/25/16 09:03 AM)


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Invisibleh0ldthedoor
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Re: Making a liquid culture with a mushroom cap [Re: Bobabouy]
    #23474853 - 07/25/16 09:13 AM (7 years, 6 months ago)

Quote:

Bobabouy said:
Can never have enough aids. As long as it's in Africa.



:notyou:


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

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Offlinemrmazdarx9
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Re: Making a liquid culture with a mushroom cap [Re: where_did_Panda_go]
    #23474864 - 07/25/16 09:19 AM (7 years, 6 months ago)

Quote:

where_did_Panda_go said:
Nah I want to see what happens, the world needs more love and aids anyway. Plus I can't throw it away, it's staring at me not to do it...





Looks like a homemade real boob feeder for babies or something out of texas chainsaw massacre


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Edited by mrmazdarx9 (07/25/16 09:24 AM)


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InvisiblePastywhyteMDiscord
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Re: Making a liquid culture with a mushroom cap [Re: mrmazdarx9]
    #23474873 - 07/25/16 09:22 AM (7 years, 6 months ago)

:popcorn:


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Invisiblemupetmower
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Re: Making a liquid culture with a mushroom cap [Re: Pastywhyte]
    #23474880 - 07/25/16 09:24 AM (7 years, 6 months ago)

Quote:

Pastywhyte said:
:popcorn:






damn you, nice ninja edit! was gonna add on the countless amounts of bacteria and mold spores to it =p


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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InvisiblePastywhyteMDiscord
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Re: Making a liquid culture with a mushroom cap [Re: mupetmower]
    #23474886 - 07/25/16 09:25 AM (7 years, 6 months ago)

:ninjawe:

Funnier this way. I actually want to see this play out lol.


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Offlinemrmazdarx9
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Re: Making a liquid culture with a mushroom cap [Re: Pastywhyte]
    #23474899 - 07/25/16 09:28 AM (7 years, 6 months ago)

You and muppetmower need to save your powers till you see my awesome agar attempt


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Invisiblemupetmower
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Re: Making a liquid culture with a mushroom cap [Re: mrmazdarx9]
    #23474910 - 07/25/16 09:31 AM (7 years, 6 months ago)

i have no powers =\

but you'll do fine, man. you will get the hang of it. if i can manage to get some healthy plates from transfers, then you can too. anyone can.


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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InvisiblePastywhyteMDiscord
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Re: Making a liquid culture with a mushroom cap [Re: mupetmower]
    #23474923 - 07/25/16 09:36 AM (7 years, 6 months ago)

Yeah agar is easier than it sounds. Hardest part is just taking the plunge. Then once your doing it it get easier every time. Practice and muscle memory are 90% of the process.

There are no powers. I'm not a pro. I just muddle along like everyone else.


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Invisiblemupetmower
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Re: Making a liquid culture with a mushroom cap [Re: Pastywhyte]
    #23474926 - 07/25/16 09:37 AM (7 years, 6 months ago)

well... that's not true. you sir, are very much a pro. and we all learn from you every day. just think you should know that.

but enough stroking =p


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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InvisibleMad Season
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Re: Making a liquid culture with a mushroom cap [Re: where_did_Panda_go]
    #23474935 - 07/25/16 09:40 AM (7 years, 6 months ago)

Quote:

where_did_Panda_go said:
Nah I want to see what happens, the world needs more love and aids anyway. Plus I can't throw it away, it's staring at me not to do it...





:rofl: it's your grow man :P. I hope you got some spore prints going as well lol


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InvisiblePastywhyteMDiscord
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Re: Making a liquid culture with a mushroom cap [Re: mupetmower]
    #23474944 - 07/25/16 09:42 AM (7 years, 6 months ago)

Quote:

mupetmower said:
well... that's not true. you sir, are very much a pro. and we all learn from you every day. just think you should know that.





Naw man. Paul stamets is a pro. John Holliday is a pro. Mark R Keith was a pro. Much as I might disagree with many of their positions on things, those guys did it for real. I am a simple hobbiest.


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Offlinemrmazdarx9
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Re: Making a liquid culture with a mushroom cap [Re: Pastywhyte]
    #23474956 - 07/25/16 09:48 AM (7 years, 6 months ago)

I like the things you two are saying but youve yet to see my thread :lol:


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InvisibleSupalemonhaze
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Re: Making a liquid culture with a mushroom cap [Re: mrmazdarx9]
    #23475043 - 07/25/16 10:17 AM (7 years, 6 months ago)

Oh boy, OP is keeping this thing? I think we need to link the "avoiding facepalms" thread right here.


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Offlinelukehighwalker710
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Re: Making a liquid culture with a mushroom cap [Re: Supalemonhaze]
    #23475067 - 07/25/16 10:23 AM (7 years, 6 months ago)

:rofl2:


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InvisiblePastywhyteMDiscord
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Re: Making a liquid culture with a mushroom cap [Re: Supalemonhaze]
    #23475080 - 07/25/16 10:26 AM (7 years, 6 months ago)

Quote:

Supalemonhaze said:
Oh boy, OP is keeping this thing? I think we need to link the "avoiding facepalms" thread right here.




All I know is I want pics of the results. Not just go and hide in shame. Pics dammit. This needs to be updated regularly.


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InvisibleSupalemonhaze
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Re: Making a liquid culture with a mushroom cap [Re: Pastywhyte]
    #23475095 - 07/25/16 10:30 AM (7 years, 6 months ago)

That thing is gonna create some super trich strain that will ruin all mushroom cultivation in the world. Burn it before it germinates. :onfire:


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Offlinespacechildo
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Re: Making a liquid culture with a mushroom cap [Re: Supalemonhaze]
    #23475152 - 07/25/16 10:49 AM (7 years, 6 months ago)

this can't be real? gotta be a :puppet: goofing around.


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InvisibleSupalemonhaze
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Re: Making a liquid culture with a mushroom cap [Re: spacechildo]
    #23475159 - 07/25/16 10:52 AM (7 years, 6 months ago)

I was thinking the same thing when I saw the pic named "awesome culture" or some shit. I mean, how does something like this even come to mind? Even 20 year old teks aren't this bad :rofl: At least with spores, you have a chance for success, albiet a small one.


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Invisiblewhere_did_Panda_go
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Re: Making a liquid culture with a mushroom cap [Re: Supalemonhaze]
    #23475298 - 07/25/16 11:49 AM (7 years, 6 months ago)

The bottle is from rawsome smoothies, so I dubbed it into awesome culture. I will keep updating pics of my cute baby, it will make a new civilization after all humans gets exterminating with aids. Of course it will spare its Mother.


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OfflineYerow
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Re: Making a liquid culture with a mushroom cap [Re: where_did_Panda_go]
    #23475312 - 07/25/16 11:54 AM (7 years, 6 months ago)

:mindblown:


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InvisiblePastywhyteMDiscord
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Re: Making a liquid culture with a mushroom cap [Re: where_did_Panda_go]
    #23475318 - 07/25/16 11:57 AM (7 years, 6 months ago)

So I gotta ask. . .what is your end game with this? I mean assuming that you were very careful and placed the cap over the bottle in a SAB or in front of a flowhood and left it there the whole time, the cap itself is likely going to be a vector for nasty. But even if it wasn't, 300 trillion spores is not really the way to do a good grow, you want less spores not more.

Based on what I see there I have no idea how you will asperate the LC without running major contam vectors, assuming it's a clean culture to begin with. So were you simply planning to knock up some cakes and pray? Or grain jars and take pics of the rainbows that show? You obviously know that it's not going to produce much of anything in the way of mushrooms, so what is the point?

We see people do this kind of stuff all the time. I just wonder since you know it will be unlikely to produce even a single mushroom indoors, why bother with it? Growing mold isn't hard, so far if that was your goal, you have already invested a lot more effort than you needed to. Just leave a cup of coffee on the counter for 2 weeks if you want to grow mold.


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InvisibleInocuole
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Re: Making a liquid culture with a mushroom cap [Re: where_did_Panda_go]
    #23475357 - 07/25/16 12:13 PM (7 years, 6 months ago)

Quote:

Pastywhyte said:
So I gotta ask. . .what is your end game with this? I mean assuming that you were very careful and placed the cap over the bottle in a SAB or in front of a flowhood and left it there the whole time, the cap itself is likely going to be a vector for nasty. But even if it wasn't, 300 trillion spores is not really the way to do a good grow, you want less spores not more.

Based on what I see there I have no idea how you will asperate the LC without running major contam vectors, assuming it's a clean culture to begin with. So were you simply planning to knock up some cakes and pray? Or grain jars and take pics of the rainbows that show? You obviously know that it's not going to produce much of anything in the way of mushrooms, so what is the point?

We see people do this kind of stuff all the time. I just wonder since you know it will be unlikely to produce even a single mushroom indoors, why bother with it? Growing mold isn't hard, so far if that was your goal, you have already invested a lot more effort than you needed to. Just leave a cup of coffee on the counter for 2 weeks if you want to grow mold.



Come on Pasty, they're gonna dunk their substrate in it.


Quote:

where_did_Panda_go said:
I will keep the mushroom cap on for 12 hours and will use it in the coming days to drown my prepared substrate with it.  Ok not really drown, but to use alot of inucolation holes.




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Re: Making a liquid culture with a mushroom cap [Re: Inocuole]
    #23475379 - 07/25/16 12:22 PM (7 years, 6 months ago)

yeah.. this is gonna be so damn wasteful.. why not just mail me all the sub mix that your plan to ruin and throw away, anyways... ill use it on something that will actually not be a waste, and turn into trash.


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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Re: Making a liquid culture with a mushroom cap [Re: Supalemonhaze]
    #23475476 - 07/25/16 01:07 PM (7 years, 6 months ago)

Quote:

mrmazdarx9 said:
I like the things you two are saying but youve yet to see my thread :lol:



Lets see this thread DM me

Quote:

Supalemonhaze said:
I was thinking the same thing when I saw the pic named "awesome culture" or some shit. I mean, how does something like this even come to mind? Even 20 year old teks aren't this bad :rofl: At least with spores, you have a chance for success, albiet a small one.



I have yet to have a problem with spores. I've made 4 spore syringes in open air environment with complete success so far.
Just boiled water in a microwave for 10 mins. Used the water to clean my syringes then finally let some hot water cool in the syringe.
Scraped some spores in a shot glass and mixed with cooled syringe water.
My prints were made in open air as well.
The first time I used this method I was expecting a complete fail but 4 syringes later I haven't had an issue yet


--------------------
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Re: Making a liquid culture with a mushroom cap [Re: ComebackKid]
    #23475488 - 07/25/16 01:12 PM (7 years, 6 months ago)

maybe not a complete fail, but i bet bacteria reduced your yield significantly. and if not, then youve been getting super lucky.

doesnt mean you should suggest things like this to noobs, when instead, if we know better, then we should be trying to give them every opportunity to succeed. im glad and grateful the shrooomery was here to keep me from fails from doing stuff like that.

also, i feel like this is said a lot here, but correlation does not imply causation. starting to become one of my favorite quotes.


oh, and yet is a pretty big keyword in that post, too.


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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Invisiblewhere_did_Panda_go
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Re: Making a liquid culture with a mushroom cap [Re: Inocuole]
    #23475509 - 07/25/16 01:23 PM (7 years, 6 months ago)

My end game is of course to produce mushrooms, I think it might have a shot so I will see if it does. Not one way is the correct way, there are many good written ways to do it. But I will try a different way, because there wasnt any agar pudding packs in my cupboard, or a flow hood. Yes I am lazy, but heck why not try and see what happens. The worse that can happen is that I caused an aids epidemic, big deal. I like the quote of Thomas A. Edison: "I have not failed, I've just found 10.000 ways that won't work.

Plus a little side story,  I bought a spore syringe 3 weeks ago (PES Amazonian). Well you know I didnt had any vermiculite, brown rice powder, tyvek or any type of grains that isn't rice either :frown:
I admit I don't even have a pressure cooker, maybe you can already guess with my username but I am asian and we asians only need a rice cooker. Wanna make soup, fried eggs, fluffy rice? Time to turn on my magical rice cooker!

I admit only 2 of the 8 jars made it, but I tried different ingredients as substrate. The spores didnt liked my expired rice noodles (2 jars)  and also not my speculoos cookies (2 jars). Damn those spores are picky. The rest I used was brown rice, 2 ended up too soggy and 2 survived and are consolidating.  I will show you one of my jars that is fully colonized,





And my shameful reason why I put a tin container on top of my jar.


I don't find it wasteful or useless, I didn't buy any ingredients except the spore syringe. And the ingredients I used was sitting forever collecting dust in my cupboard. Of course my way is not recommendable, but it's not impossible. I am growing for my own use, 1 successful jar will be enough to enjoy for months.


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Re: Making a liquid culture with a mushroom cap [Re: ComebackKid]
    #23475510 - 07/25/16 01:23 PM (7 years, 6 months ago)

Quote:

ComebackKid said:
I have yet to have a problem with spores. I've made 4 spore syringes in open air environment with complete success so far.
Just boiled water in a microwave for 10 mins. Used the water to clean my syringes then finally let some hot water cool in the syringe.
Scraped some spores in a shot glass and mixed with cooled syringe water.
My prints were made in open air as well.
The first time I used this method I was expecting a complete fail but 4 syringes later I haven't had an issue yet





Why would you even do this?  Surely you know better?  Is this one of those "Too busy trying to figure out if you can to stop and ask if you should" type things?

Quote:

where_did_Panda_go said:
My end game is of course to produce mushrooms, I think it might have a shot so I will see if it does. Not one way is the correct way, there are many good written ways to do it. But I will try a different way, because there wasnt any agar pudding packs in my cupboard, or a flow hood. Yes I am lazy, but heck why not try and see what happens. The worse that can happen is that I caused an aids epidemic, big deal. I like the quote of Thomas A. Edison: "I have not failed, I've just found 10.000 ways that won't work.

Plus a little side story,  I bought a spore syringe 3 weeks ago (PES Amazonian). Well you know I didnt had any vermiculite, brown rice powder, tyvek or any type of grains that isn't rice either :frown:
I admit I don't even have a pressure cooker, maybe you can already guess with my username but I am asian and we asians only need a rice cooker. Wanna make soup, fried eggs, fluffy rice? Time to turn on my magical rice cooker!

I admit only 2 of the 8 jars made it, but I tried different ingredients as substrate. The spores didnt liked my expired rice noodles (2 jars)  and also not my speculoos cookies (2 jars). Damn those spores are picky. The rest I used was brown rice, 2 ended up too soggy and 2 survived and are consolidating.  I will show you one of my jars that is fully colonized,


I don't find it wasteful or useless, I didn't buy any ingredients except the spore syringe. And the ingredients I used was sitting forever collecting dust in my cupboard. Of course my way is not recommendable, but it's not impossible. I am growing for my own use, 1 successful jar will be enough to enjoy for months.





You are gonna have to get over all these silly ass misconceptions of yours if you want to make any progress.  You learn a lot more by reading and absorbing information than you do by failing left and right.  That's not a good excuse and it's just plain bad form.  Also nobody assumed you were asian or cared in the first place.  Not relevant to cultivation, just like your rice cooker, which is also totally irrelevant to cultivation.


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Re: Making a liquid culture with a mushroom cap [Re: mupetmower]
    #23475515 - 07/25/16 01:25 PM (7 years, 6 months ago)

I like to blow things up a bit. Doing 4 runs of something that has a 25% chance of success doesn't mean that it's a proven method. If you run 100 grows that way, 25 should work out. So 4 is well within that tolerance. But you still have 75 waiting around the corner ready to fail.

People also need to remember that brf cakes are very forgiving. Unbroken colony, a substrate that is relatively dry, bacteria has a hard time with the food source. You can get away with shit on cakes that would burn you hard on grain.


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Re: Making a liquid culture with a mushroom cap [Re: mupetmower]
    #23475532 - 07/25/16 01:31 PM (7 years, 6 months ago)

You're right, I'm not recommending this method. Just simply stating that open air procedures don't necessarily mean a fail (for BRF). Although I did end up with more bacteria than usual, fruiting didn't seem to be an issue (based on my very limited experience)
The only reason I was making these syringes was because I was waiting for my agar powder and micropore tape in the mail. And I wanted more shrooms.

Of course, even if this LC somehow magically works out there are a lot better and easier methods to make LC from spores.


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Re: Making a liquid culture with a mushroom cap [Re: ComebackKid]
    #23475540 - 07/25/16 01:34 PM (7 years, 6 months ago)

Fail is a relative concept.

This tub was a fail in my eyes. Full of bacteria and BE was totally off.




This one was better. Still not my best yield but within range.



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Re: Making a liquid culture with a mushroom cap [Re: ComebackKid]
    #23475544 - 07/25/16 01:35 PM (7 years, 6 months ago)

Well we're talking about a LC here.  This is like, astronomical disastrous failure.  BRF cakes are the most resistant to contamination, LC are on the far opposite end of the spectrum.

Sorry for putting things quite so bluntly, asian panda person, you have to understand how much we try to avoid people failing and wasting their time and ours.  You need a pressure cooker if you want to be making LCs and shit.  With your tools and experience the only thing you should be doing is making spore syringes and brf cakes.

Fully imagine the feeling of investing your time in giving advice and having it ignored in favor of dumb shit, every day, and you might see why I'm so snappy.


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Re: Making a liquid culture with a mushroom cap [Re: Inocuole]
    #23475554 - 07/25/16 01:39 PM (7 years, 6 months ago)

Doing sterile tech in open air is as dumb as growing MJ under a 30w incandescent bulb..
yeah "it works" but you're simply stealing from yourself and its just as easy to work inside a SAB as it is to plug in the right bulb for your weed.
But as soon as we're talking about mushrooms people think yey 1 mushroom I won, while if they grew a plant and got 0,2g seed-weed they'd know they fucked up.


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Re: Making a liquid culture with a mushroom cap [Re: Inocuole]
    #23475562 - 07/25/16 01:41 PM (7 years, 6 months ago)

Alfred Pennyworth:" Well, because he thought it was good sport. Because men aren't looking for anything logical, like money. They can't be bought, bullied, reasoned, or negotiated with. .some men just want to watch the world burn"
stupid quote stupid thread Batman Rules


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Re: Making a liquid culture with a mushroom cap [Re: mrmazdarx9]
    #23475565 - 07/25/16 01:42 PM (7 years, 6 months ago)

If only I could just let it all go and be like that. :shrug:  Caring is a burden I find it hard to wish on anyone.


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Re: Making a liquid culture with a mushroom cap [Re: Inocuole]
    #23475576 - 07/25/16 01:45 PM (7 years, 6 months ago)

Quote:

Inocuole said:
If only I could just let it all go and be like that. :shrug:  Caring is a burden I find it hard to wish on anyone.



Inocuole you old softy you
look on the bright side this thread pushed you over 20000 posts


Edited by mrmazdarx9 (07/25/16 01:46 PM)


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Re: Making a liquid culture with a mushroom cap [Re: Pastywhyte]
    #23475626 - 07/25/16 02:04 PM (7 years, 6 months ago)

I am not aiming for 100% succes, 25 percent or even less is good enough for me. Why should I buy all those stuff if I just want enough gram for myself for a month or two. I am not collecting mushrooms like pokemon, I am actually planning to eat them. Inocuole I am glad nobody cares about my etnicity or my rice cooker. Good to know racism is losing its battle. Failure is fine, you learn best with failure.  I am truly sorry if I have upset anybody because I didnt follow the mushroom cultivation bible. If it becomes a astronomical failure or a lucky miracle, who wants to follow it?:thumbup:


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Re: Making a liquid culture with a mushroom cap [Re: where_did_Panda_go]
    #23475651 - 07/25/16 02:13 PM (7 years, 6 months ago)

No one is upset at least I'm not, hell its your grow not mine. But understand that in this hobby failure compounds itself. You might get away with sloppy work a few times. But as sporeload rise, and you have no foundation to build on, you might find even getting a gram or two challenging. Do things right, or face the possibility of having no success in the future.

I doubt that LC mess is going to produce even a single gram.


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Re: Making a liquid culture with a mushroom cap [Re: where_did_Panda_go]
    #23475679 - 07/25/16 02:20 PM (7 years, 6 months ago)

This is like watching a guy take a unicycle from Seattle to Los Angeles on the Interstate 5.  Medics are standing by.
:popcorn:


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Re: Making a liquid culture with a mushroom cap [Re: Pastywhyte]
    #23475688 - 07/25/16 02:24 PM (7 years, 6 months ago)

Quote:

where_did_Panda_go said:Why should I buy all those stuff if I just want enough gram for myself for a month or two.




Why use a timer when you cook? To make sure the food turns out right.

Same idea applies here. I understand about doing something purely as an experiment (just check my history). But when you're trying to accomplish something your own way that other people are already doing "right" (following generally accepted procedure and guidelines) and in doing so are hearing consistent criticisms that transcend personal preferences, one might step back and re-evaluate what theyre doing to begin with.

If you want a good shot at success without going all-out, just scale back the recipe for brf cakes and make a smaller batch. You don't need a PC for pf, either. From what I hear it's a great tek for beginners and does not involve a big expensive shopping list.

:2cents:


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Re: Making a liquid culture with a mushroom cap [Re: h0ldthedoor]
    #23475701 - 07/25/16 02:29 PM (7 years, 6 months ago)

One thing the OP might not understand is that LC by design is not for small amounts. The whole point of LC is exponentially expanding a colony. I don't bother with LC at all unless I plan to run at least half a dozen 66 quart monos with it.

If you just want to stretch spores then sterilize a quart of water with a lid outfitted with a SHIP. Then carefully make or buy a spore syringe and fire the whole works into the quart of water. Spores are then stretched.


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Re: Making a liquid culture with a mushroom cap [Re: Pastywhyte]
    #23475838 - 07/25/16 03:17 PM (7 years, 6 months ago)

Bwahaha I leave for a few months and come back to find a post like this, my faith in humanity has been restored! Love it :derp:


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Re: Making a liquid culture with a mushroom cap [Re: MrSturgill]
    #23475840 - 07/25/16 03:18 PM (7 years, 6 months ago)

It's gotten really bad the past few weeks/months I feel like, tons of trolls and people who like learning the hard way.


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Re: Making a liquid culture with a mushroom cap [Re: Inocuole]
    #23475847 - 07/25/16 03:22 PM (7 years, 6 months ago)

Hey man long time, it has always been pretty bad but this is just on a whole new level.


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Re: Making a liquid culture with a mushroom cap [Re: Inocuole]
    #23475884 - 07/25/16 03:39 PM (7 years, 6 months ago)

Like The whining guy whos posted something on advanced mycology that is nothing to do with advanced mycology is doing my nut in attention seeking troll


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Edited by mrmazdarx9 (07/25/16 03:47 PM)


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Re: Making a liquid culture with a mushroom cap [Re: mrmazdarx9]
    #23475895 - 07/25/16 03:46 PM (7 years, 6 months ago)

Lol yeah that guy's somethin else.


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Re: Making a liquid culture with a mushroom cap [Re: MrSturgill]
    #23475896 - 07/25/16 03:48 PM (7 years, 6 months ago)

Quote:

MrSturgill said:
Lol yeah that guy's somethin else.



Thank you I was trying to figure out if im being a prick or not


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Re: Making a liquid culture with a mushroom cap [Re: mrmazdarx9]
    #23478095 - 07/26/16 08:59 AM (7 years, 6 months ago)

Guess I call this day one



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Re: Making a liquid culture with a mushroom cap [Re: where_did_Panda_go]
    #23478139 - 07/26/16 09:22 AM (7 years, 6 months ago)

Wow! An Assian Topsy!! :threadmonitor:


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Re: Making a liquid culture with a mushroom cap [Re: where_did_Panda_go]
    #23478154 - 07/26/16 09:28 AM (7 years, 6 months ago)

Quote:

where_did_Panda_go said:
Guess I call this day one






Why are you even doing this though?  Really, start on something else now.  Waiting around on this is going to be a waste of time.  You cannot expose sugar water to open air and expect other shit not to grow in it.  Even a single bacterium can ruin a LC, with thousands falling in there, it's really got no chance at all.


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Re: Making a liquid culture with a mushroom cap [Re: Inocuole]
    #23478165 - 07/26/16 09:32 AM (7 years, 6 months ago)

no use trying to reason, here.


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Re: Making a liquid culture with a mushroom cap [Re: Inocuole]
    #23478182 - 07/26/16 09:37 AM (7 years, 6 months ago)

Quote:

Inocuole said:
Quote:

where_did_Panda_go said:
Guess I call this day one






Why are you even doing this though?  Really, start on something else now.  Waiting around on this is going to be a waste of time.  You cannot expose sugar water to open air and expect other shit not to grow in it.  Even a single bacterium can ruin a LC, with thousands falling in there, it's really got no chance at all.




Its like when little kids make magic potions from hose water and grass clippings. Use yer imagination mang. . .


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Re: Making a liquid culture with a mushroom cap [Re: Pastywhyte]
    #23478231 - 07/26/16 09:57 AM (7 years, 6 months ago)

Quote:

Pastywhyte said:
Quote:

Inocuole said:
Quote:

where_did_Panda_go said:
Guess I call this day one






Why are you even doing this though?  Really, start on something else now.  Waiting around on this is going to be a waste of time.  You cannot expose sugar water to open air and expect other shit not to grow in it.  Even a single bacterium can ruin a LC, with thousands falling in there, it's really got no chance at all.




Its like when little kids make magic potions from hose water and grass clippings. Use yer imagination mang. . .



:dumblol:  :rofl:


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Re: Making a liquid culture with a mushroom cap [Re: Pastywhyte]
    #23478244 - 07/26/16 10:01 AM (7 years, 6 months ago)

Well, I'm pretty sure he's going to grow some mashrooms, please people, do not discourage him with your sarcasm, for science's sake!


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Re: Making a liquid culture with a mushroom cap [Re: Josex]
    #23478294 - 07/26/16 10:27 AM (7 years, 6 months ago)

We may or may not be dealing with a super attractive but unfortunately not very sharp asian chick, based on gallery/journal.


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Re: Making a liquid culture with a mushroom cap [Re: Inocuole]
    #23478300 - 07/26/16 10:28 AM (7 years, 6 months ago)

I'm a doctor of journalism. . .

:fearandloathing:


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Re: Making a liquid culture with a mushroom cap [Re: Josex]
    #23478301 - 07/26/16 10:29 AM (7 years, 6 months ago)



Day 2 on my LC.

Pressure cooked this water bottle for 24 hours at 20psi.

Inoculated it with a contamed BRF cake...


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Re: Making a liquid culture with a mushroom cap [Re: morty422]
    #23478306 - 07/26/16 10:33 AM (7 years, 6 months ago)

Idk if 24 hours is long enough..


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Re: Making a liquid culture with a mushroom cap [Re: Inocuole]
    #23478324 - 07/26/16 10:41 AM (7 years, 6 months ago)

Quote:

Inocuole said:
We may or may not be dealing with a super attractive but unfortunately not very sharp asian chick, based on gallery/journal.



Wait... WHAT!! I retract all negative things I said
Quote:

where_did_Panda_go said:
Guess I call this day one







Hey that looks brilliant whatever your doing anyway...



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Edited by mrmazdarx9 (07/26/16 10:44 AM)


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Re: Making a liquid culture with a mushroom cap [Re: Mad Season]
    #23478479 - 07/26/16 11:47 AM (7 years, 6 months ago)

Quote:

Mad Season said:
Idk if 24 hours is long enough..




I could put it back in the PC for a while right?
That won't hurt anything...
Also,
Is this cobweb?


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Offlinemrmazdarx9
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Re: Making a liquid culture with a mushroom cap [Re: morty422]
    #23478514 - 07/26/16 12:04 PM (7 years, 6 months ago)

Quote:

morty422 said:
Quote:

Mad Season said:
Idk if 24 hours is long enough..




I could put it back in the PC for a while right?
That won't hurt anything...
Also,
Is this cobweb?




Ah man think I got it too

And dog hair mold plus brown forest branch maybe its just wall myc


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Re: Making a liquid culture with a mushroom cap [Re: mrmazdarx9]
    #23478933 - 07/26/16 02:19 PM (7 years, 6 months ago)

:thisisterrible:


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Invisiblewhere_did_Panda_go
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Re: Making a liquid culture with a mushroom cap [Re: Supalemonhaze]
    #23482151 - 07/27/16 11:55 AM (7 years, 6 months ago)

Well might as well keep it, who knows a beautiful rainbow in a bottle will appear. I can then send it as a peace offering to inocuele. Well I can try something else, but according to the information available, it will be all set for failure without a pressure cooker anyway.


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Re: Making a liquid culture with a mushroom cap [Re: where_did_Panda_go]
    #23482155 - 07/27/16 11:58 AM (7 years, 6 months ago)

How did you make those mushrooms? You didn't use a pc.. PF tek doesn't need a PC. Agar can be boiled to sterilize. A PC is needed for grain spawn.


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Re: Making a liquid culture with a mushroom cap [Re: Mad Season]
    #23484379 - 07/28/16 02:20 AM (7 years, 6 months ago)

I started with a growkit. I thought with PF tek you add a layer of vermiculite on top. How am I suppose to throw a wedge of agar mycelium in there if the vermiculite is being used as a barrier? Or do I need the mycelium from agar to make a spore syringe first...


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Re: Making a liquid culture with a mushroom cap [Re: where_did_Panda_go]
    #23484389 - 07/28/16 02:28 AM (7 years, 6 months ago)

You do PF tek with syringes, and you do grains with agar.  You do add a dry vermiculite layer with PF tek.  Right now you need to pick one method, and stick with it.  Steps that work for one method will completely fuck up another based on small details you might not be able to appreciate.

PF tek: https://www.shroomery.org/forums/showflat.php/Number/11585613#11585613


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