|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
|
Re: Making a liquid culture with a mushroom cap [Re: Inocuole]
#23478182 - 07/26/16 09:37 AM (7 years, 6 months ago) |
|
|
Quote:
Inocuole said:
Quote:
where_did_Panda_go said: Guess I call this day one

Why are you even doing this though? Really, start on something else now. Waiting around on this is going to be a waste of time. You cannot expose sugar water to open air and expect other shit not to grow in it. Even a single bacterium can ruin a LC, with thousands falling in there, it's really got no chance at all.
Its like when little kids make magic potions from hose water and grass clippings. Use yer imagination mang. . .
|
lukehighwalker710
Cloud 9 Dweller



Registered: 03/04/16
Posts: 609
Loc: out there
Last seen: 6 years, 22 days
|
Re: Making a liquid culture with a mushroom cap [Re: Pastywhyte]
#23478231 - 07/26/16 09:57 AM (7 years, 6 months ago) |
|
|
Quote:
Pastywhyte said:
Quote:
Inocuole said:
Quote:
where_did_Panda_go said: Guess I call this day one

Why are you even doing this though? Really, start on something else now. Waiting around on this is going to be a waste of time. You cannot expose sugar water to open air and expect other shit not to grow in it. Even a single bacterium can ruin a LC, with thousands falling in there, it's really got no chance at all.
Its like when little kids make magic potions from hose water and grass clippings. Use yer imagination mang. . .
--------------------
Highwalker
|
Josex
#cheat_code



Registered: 11/13/15
Posts: 8,995
Loc:
|
Re: Making a liquid culture with a mushroom cap [Re: Pastywhyte]
#23478244 - 07/26/16 10:01 AM (7 years, 6 months ago) |
|
|
Well, I'm pretty sure he's going to grow some mashrooms, please people, do not discourage him with your sarcasm, for science's sake!
|
Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
|
Re: Making a liquid culture with a mushroom cap [Re: Josex]
#23478294 - 07/26/16 10:27 AM (7 years, 6 months ago) |
|
|
We may or may not be dealing with a super attractive but unfortunately not very sharp asian chick, based on gallery/journal.
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
|
Re: Making a liquid culture with a mushroom cap [Re: Inocuole]
#23478300 - 07/26/16 10:28 AM (7 years, 6 months ago) |
|
|
I'm a doctor of journalism. . .
|
morty422
Wuba-luba-dub-dub!



Registered: 07/06/16
Posts: 988
|
Re: Making a liquid culture with a mushroom cap [Re: Josex]
#23478301 - 07/26/16 10:29 AM (7 years, 6 months ago) |
|
|

Day 2 on my LC.
Pressure cooked this water bottle for 24 hours at 20psi.
Inoculated it with a contamed BRF cake...
|
Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
|
Re: Making a liquid culture with a mushroom cap [Re: morty422]
#23478306 - 07/26/16 10:33 AM (7 years, 6 months ago) |
|
|
Idk if 24 hours is long enough..
|
mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
|
Re: Making a liquid culture with a mushroom cap [Re: Inocuole]
#23478324 - 07/26/16 10:41 AM (7 years, 6 months ago) |
|
|
Quote:
Inocuole said: We may or may not be dealing with a super attractive but unfortunately not very sharp asian chick, based on gallery/journal.
Wait... WHAT!! I retract all negative things I said
Quote:
where_did_Panda_go said: Guess I call this day one

Hey that looks brilliant whatever your doing anyway...
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
Edited by mrmazdarx9 (07/26/16 10:44 AM)
|
morty422
Wuba-luba-dub-dub!



Registered: 07/06/16
Posts: 988
|
Re: Making a liquid culture with a mushroom cap [Re: Mad Season]
#23478479 - 07/26/16 11:47 AM (7 years, 6 months ago) |
|
|
Quote:
Mad Season said: Idk if 24 hours is long enough..
I could put it back in the PC for a while right? That won't hurt anything... Also, Is this cobweb?
|
mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
|
Re: Making a liquid culture with a mushroom cap [Re: morty422]
#23478514 - 07/26/16 12:04 PM (7 years, 6 months ago) |
|
|
Quote:
morty422 said:
Quote:
Mad Season said: Idk if 24 hours is long enough..
I could put it back in the PC for a while right? That won't hurt anything... Also, Is this cobweb?

Ah man think I got it too
 And dog hair mold plus brown forest branch maybe its just wall myc
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
|
Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
|
Re: Making a liquid culture with a mushroom cap [Re: mrmazdarx9]
#23478933 - 07/26/16 02:19 PM (7 years, 6 months ago) |
|
|
|
where_did_Panda_go
Aspiring psychonaut


Registered: 06/21/16
Posts: 10
Loc: The Netherlands
|
Re: Making a liquid culture with a mushroom cap [Re: Supalemonhaze]
#23482151 - 07/27/16 11:55 AM (7 years, 6 months ago) |
|
|
Well might as well keep it, who knows a beautiful rainbow in a bottle will appear. I can then send it as a peace offering to inocuele. Well I can try something else, but according to the information available, it will be all set for failure without a pressure cooker anyway.
|
Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
|
|
How did you make those mushrooms? You didn't use a pc.. PF tek doesn't need a PC. Agar can be boiled to sterilize. A PC is needed for grain spawn.
|
where_did_Panda_go
Aspiring psychonaut


Registered: 06/21/16
Posts: 10
Loc: The Netherlands
|
Re: Making a liquid culture with a mushroom cap [Re: Mad Season]
#23484379 - 07/28/16 02:20 AM (7 years, 6 months ago) |
|
|
I started with a growkit. I thought with PF tek you add a layer of vermiculite on top. How am I suppose to throw a wedge of agar mycelium in there if the vermiculite is being used as a barrier? Or do I need the mycelium from agar to make a spore syringe first...
|
Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
|
|
You do PF tek with syringes, and you do grains with agar. You do add a dry vermiculite layer with PF tek. Right now you need to pick one method, and stick with it. Steps that work for one method will completely fuck up another based on small details you might not be able to appreciate.
PF tek: https://www.shroomery.org/forums/showflat.php/Number/11585613#11585613
|
|