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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Making a liquid culture with a mushroom cap [Re: mupetmower]
#23474944 - 07/25/16 09:42 AM (7 years, 6 months ago) |
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Quote:
mupetmower said: well... that's not true. you sir, are very much a pro. and we all learn from you every day. just think you should know that.
Naw man. Paul stamets is a pro. John Holliday is a pro. Mark R Keith was a pro. Much as I might disagree with many of their positions on things, those guys did it for real. I am a simple hobbiest.
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mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
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Re: Making a liquid culture with a mushroom cap [Re: Pastywhyte]
#23474956 - 07/25/16 09:48 AM (7 years, 6 months ago) |
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I like the things you two are saying but youve yet to see my thread
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Making a liquid culture with a mushroom cap [Re: mrmazdarx9]
#23475043 - 07/25/16 10:17 AM (7 years, 6 months ago) |
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Oh boy, OP is keeping this thing? I think we need to link the "avoiding facepalms" thread right here.
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lukehighwalker710
Cloud 9 Dweller



Registered: 03/04/16
Posts: 609
Loc: out there
Last seen: 6 years, 22 days
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Re: Making a liquid culture with a mushroom cap [Re: Supalemonhaze]
#23475067 - 07/25/16 10:23 AM (7 years, 6 months ago) |
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Highwalker
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Making a liquid culture with a mushroom cap [Re: Supalemonhaze]
#23475080 - 07/25/16 10:26 AM (7 years, 6 months ago) |
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Quote:
Supalemonhaze said: Oh boy, OP is keeping this thing? I think we need to link the "avoiding facepalms" thread right here.
All I know is I want pics of the results. Not just go and hide in shame. Pics dammit. This needs to be updated regularly.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Making a liquid culture with a mushroom cap [Re: Pastywhyte]
#23475095 - 07/25/16 10:30 AM (7 years, 6 months ago) |
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That thing is gonna create some super trich strain that will ruin all mushroom cultivation in the world. Burn it before it germinates.
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Making a liquid culture with a mushroom cap [Re: Supalemonhaze]
#23475152 - 07/25/16 10:49 AM (7 years, 6 months ago) |
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this can't be real? gotta be a goofing around.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Making a liquid culture with a mushroom cap [Re: spacechildo]
#23475159 - 07/25/16 10:52 AM (7 years, 6 months ago) |
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I was thinking the same thing when I saw the pic named "awesome culture" or some shit. I mean, how does something like this even come to mind? Even 20 year old teks aren't this bad At least with spores, you have a chance for success, albiet a small one.
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where_did_Panda_go
Aspiring psychonaut


Registered: 06/21/16
Posts: 10
Loc: The Netherlands
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Re: Making a liquid culture with a mushroom cap [Re: Supalemonhaze]
#23475298 - 07/25/16 11:49 AM (7 years, 6 months ago) |
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The bottle is from rawsome smoothies, so I dubbed it into awesome culture. I will keep updating pics of my cute baby, it will make a new civilization after all humans gets exterminating with aids. Of course it will spare its Mother.
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Yerow
Stranger



Registered: 09/22/14
Posts: 1,206
Last seen: 2 years, 7 months
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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So I gotta ask. . .what is your end game with this? I mean assuming that you were very careful and placed the cap over the bottle in a SAB or in front of a flowhood and left it there the whole time, the cap itself is likely going to be a vector for nasty. But even if it wasn't, 300 trillion spores is not really the way to do a good grow, you want less spores not more.
Based on what I see there I have no idea how you will asperate the LC without running major contam vectors, assuming it's a clean culture to begin with. So were you simply planning to knock up some cakes and pray? Or grain jars and take pics of the rainbows that show? You obviously know that it's not going to produce much of anything in the way of mushrooms, so what is the point?
We see people do this kind of stuff all the time. I just wonder since you know it will be unlikely to produce even a single mushroom indoors, why bother with it? Growing mold isn't hard, so far if that was your goal, you have already invested a lot more effort than you needed to. Just leave a cup of coffee on the counter for 2 weeks if you want to grow mold.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Quote:
Pastywhyte said: So I gotta ask. . .what is your end game with this? I mean assuming that you were very careful and placed the cap over the bottle in a SAB or in front of a flowhood and left it there the whole time, the cap itself is likely going to be a vector for nasty. But even if it wasn't, 300 trillion spores is not really the way to do a good grow, you want less spores not more.
Based on what I see there I have no idea how you will asperate the LC without running major contam vectors, assuming it's a clean culture to begin with. So were you simply planning to knock up some cakes and pray? Or grain jars and take pics of the rainbows that show? You obviously know that it's not going to produce much of anything in the way of mushrooms, so what is the point?
We see people do this kind of stuff all the time. I just wonder since you know it will be unlikely to produce even a single mushroom indoors, why bother with it? Growing mold isn't hard, so far if that was your goal, you have already invested a lot more effort than you needed to. Just leave a cup of coffee on the counter for 2 weeks if you want to grow mold.
Come on Pasty, they're gonna dunk their substrate in it.
Quote:
where_did_Panda_go said: I will keep the mushroom cap on for 12 hours and will use it in the coming days to drown my prepared substrate with it. Ok not really drown, but to use alot of inucolation holes.
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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Re: Making a liquid culture with a mushroom cap [Re: Inocuole]
#23475379 - 07/25/16 12:22 PM (7 years, 6 months ago) |
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yeah.. this is gonna be so damn wasteful.. why not just mail me all the sub mix that your plan to ruin and throw away, anyways... ill use it on something that will actually not be a waste, and turn into trash.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Making a liquid culture with a mushroom cap [Re: Supalemonhaze]
#23475476 - 07/25/16 01:07 PM (7 years, 6 months ago) |
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Quote:
mrmazdarx9 said: I like the things you two are saying but youve yet to see my thread 
Lets see this thread DM me
Quote:
Supalemonhaze said: I was thinking the same thing when I saw the pic named "awesome culture" or some shit. I mean, how does something like this even come to mind? Even 20 year old teks aren't this bad At least with spores, you have a chance for success, albiet a small one.
I have yet to have a problem with spores. I've made 4 spore syringes in open air environment with complete success so far. Just boiled water in a microwave for 10 mins. Used the water to clean my syringes then finally let some hot water cool in the syringe. Scraped some spores in a shot glass and mixed with cooled syringe water. My prints were made in open air as well. The first time I used this method I was expecting a complete fail but 4 syringes later I haven't had an issue yet
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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Re: Making a liquid culture with a mushroom cap [Re: ComebackKid]
#23475488 - 07/25/16 01:12 PM (7 years, 6 months ago) |
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maybe not a complete fail, but i bet bacteria reduced your yield significantly. and if not, then youve been getting super lucky.
doesnt mean you should suggest things like this to noobs, when instead, if we know better, then we should be trying to give them every opportunity to succeed. im glad and grateful the shrooomery was here to keep me from fails from doing stuff like that.
also, i feel like this is said a lot here, but correlation does not imply causation. starting to become one of my favorite quotes.
oh, and yet is a pretty big keyword in that post, too.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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where_did_Panda_go
Aspiring psychonaut


Registered: 06/21/16
Posts: 10
Loc: The Netherlands
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Re: Making a liquid culture with a mushroom cap [Re: Inocuole]
#23475509 - 07/25/16 01:23 PM (7 years, 6 months ago) |
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My end game is of course to produce mushrooms, I think it might have a shot so I will see if it does. Not one way is the correct way, there are many good written ways to do it. But I will try a different way, because there wasnt any agar pudding packs in my cupboard, or a flow hood. Yes I am lazy, but heck why not try and see what happens. The worse that can happen is that I caused an aids epidemic, big deal. I like the quote of Thomas A. Edison: "I have not failed, I've just found 10.000 ways that won't work.
Plus a little side story, I bought a spore syringe 3 weeks ago (PES Amazonian). Well you know I didnt had any vermiculite, brown rice powder, tyvek or any type of grains that isn't rice either  I admit I don't even have a pressure cooker, maybe you can already guess with my username but I am asian and we asians only need a rice cooker. Wanna make soup, fried eggs, fluffy rice? Time to turn on my magical rice cooker!
I admit only 2 of the 8 jars made it, but I tried different ingredients as substrate. The spores didnt liked my expired rice noodles (2 jars) and also not my speculoos cookies (2 jars). Damn those spores are picky. The rest I used was brown rice, 2 ended up too soggy and 2 survived and are consolidating. I will show you one of my jars that is fully colonized,


And my shameful reason why I put a tin container on top of my jar.

I don't find it wasteful or useless, I didn't buy any ingredients except the spore syringe. And the ingredients I used was sitting forever collecting dust in my cupboard. Of course my way is not recommendable, but it's not impossible. I am growing for my own use, 1 successful jar will be enough to enjoy for months.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Making a liquid culture with a mushroom cap [Re: ComebackKid]
#23475510 - 07/25/16 01:23 PM (7 years, 6 months ago) |
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Quote:
ComebackKid said: I have yet to have a problem with spores. I've made 4 spore syringes in open air environment with complete success so far. Just boiled water in a microwave for 10 mins. Used the water to clean my syringes then finally let some hot water cool in the syringe. Scraped some spores in a shot glass and mixed with cooled syringe water. My prints were made in open air as well. The first time I used this method I was expecting a complete fail but 4 syringes later I haven't had an issue yet
Why would you even do this? Surely you know better? Is this one of those "Too busy trying to figure out if you can to stop and ask if you should" type things?
Quote:
where_did_Panda_go said: My end game is of course to produce mushrooms, I think it might have a shot so I will see if it does. Not one way is the correct way, there are many good written ways to do it. But I will try a different way, because there wasnt any agar pudding packs in my cupboard, or a flow hood. Yes I am lazy, but heck why not try and see what happens. The worse that can happen is that I caused an aids epidemic, big deal. I like the quote of Thomas A. Edison: "I have not failed, I've just found 10.000 ways that won't work.
Plus a little side story, I bought a spore syringe 3 weeks ago (PES Amazonian). Well you know I didnt had any vermiculite, brown rice powder, tyvek or any type of grains that isn't rice either  I admit I don't even have a pressure cooker, maybe you can already guess with my username but I am asian and we asians only need a rice cooker. Wanna make soup, fried eggs, fluffy rice? Time to turn on my magical rice cooker!
I admit only 2 of the 8 jars made it, but I tried different ingredients as substrate. The spores didnt liked my expired rice noodles (2 jars) and also not my speculoos cookies (2 jars). Damn those spores are picky. The rest I used was brown rice, 2 ended up too soggy and 2 survived and are consolidating. I will show you one of my jars that is fully colonized,
I don't find it wasteful or useless, I didn't buy any ingredients except the spore syringe. And the ingredients I used was sitting forever collecting dust in my cupboard. Of course my way is not recommendable, but it's not impossible. I am growing for my own use, 1 successful jar will be enough to enjoy for months.
You are gonna have to get over all these silly ass misconceptions of yours if you want to make any progress. You learn a lot more by reading and absorbing information than you do by failing left and right. That's not a good excuse and it's just plain bad form. Also nobody assumed you were asian or cared in the first place. Not relevant to cultivation, just like your rice cooker, which is also totally irrelevant to cultivation.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Making a liquid culture with a mushroom cap [Re: mupetmower]
#23475515 - 07/25/16 01:25 PM (7 years, 6 months ago) |
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I like to blow things up a bit. Doing 4 runs of something that has a 25% chance of success doesn't mean that it's a proven method. If you run 100 grows that way, 25 should work out. So 4 is well within that tolerance. But you still have 75 waiting around the corner ready to fail.
People also need to remember that brf cakes are very forgiving. Unbroken colony, a substrate that is relatively dry, bacteria has a hard time with the food source. You can get away with shit on cakes that would burn you hard on grain.
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Making a liquid culture with a mushroom cap [Re: mupetmower]
#23475532 - 07/25/16 01:31 PM (7 years, 6 months ago) |
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You're right, I'm not recommending this method. Just simply stating that open air procedures don't necessarily mean a fail (for BRF). Although I did end up with more bacteria than usual, fruiting didn't seem to be an issue (based on my very limited experience) The only reason I was making these syringes was because I was waiting for my agar powder and micropore tape in the mail. And I wanted more shrooms.
Of course, even if this LC somehow magically works out there are a lot better and easier methods to make LC from spores.
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Making a liquid culture with a mushroom cap [Re: ComebackKid]
#23475540 - 07/25/16 01:34 PM (7 years, 6 months ago) |
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Fail is a relative concept.
This tub was a fail in my eyes. Full of bacteria and BE was totally off.

This one was better. Still not my best yield but within range.
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