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Invisiblewhere_did_Panda_go
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Making a liquid culture with a mushroom cap
    #23474393 - 07/25/16 03:40 AM (7 years, 6 months ago)

Hi,

Newbie here, I started from a growkit and decided that it could be cheaper to grow my own mushrooms by doing everything myself. So I let some mushrooms mature and decided to make some spore prints and a liquid culture.
For the liquid culture it sounded a bit tedious to first start with spore print, then scrape some of with a sterilized scraper, whatever it is called and drop it into the liquid culture or syringe it first (If I haven't screwed up by contaminating my spore print that is). So I spotted a cute glass of bottle, put some water and little honey in it and let it pressure cook for 1.30 hour. After cooling down, I decided to change the bottle cap to a mushroom cap. After 3 hours it looked like this.



I will keep the mushroom cap on for 12 hours and will use it in the coming days to drown my prepared substrate with it.  Ok not really drown, but to use alot of inucolation holes.


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InvisibleInocuole
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Re: Making a liquid culture with a mushroom cap [Re: where_did_Panda_go]
    #23474400 - 07/25/16 03:43 AM (7 years, 6 months ago)

Oh goodness no that is absolutely not how you make an LC, that is already ruined.

1. Inoculating LC with spores is reckless because spores aren't sterile and it's sugar water we're talking about here.
2. If you were to inoculate anything with spores, you'd use like, a tiny speck, you'd absolutely never let a whole cap sporulate into your medium, and especially not in.....
3. Open air
4. Drown.. the substrate?  I'm pretty sure you're trolling now.

Here.. so you're not like "But that's not helpful"

http://www.shroomery.org/forums/showflat.php/Number/19140341#19140341
https://www.shroomery.org/forums/showflat.php/Number/22721954
https://www.shroomery.org/forums/showflat.php/Number/21448273

Don't mess with LC until you've mastered agar.  Very important intermediate step there.


Edited by Inocuole (07/25/16 05:15 AM)


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Invisiblewhere_did_Panda_go
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Re: Making a liquid culture with a mushroom cap [Re: Inocuole]
    #23474543 - 07/25/16 06:16 AM (7 years, 6 months ago)

If spores are never sterile, how would it be able to not contaminate on agar? I read somewhere in recent posts agar gives spores more favorable environment, but do I really also need to add sugar and starch to an agar mixture used for desserts? Sugar no problem, but do I need to boil a potato to get starch?

I assumed that the mushroom cap is covering my bottle, so only spores can drop into the liquid culture. Or do you think this might had a better chance if I only used sterilized water? Most spore syringe only contain water and spores, would that not be a problem with contamination too?


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OfflineYerow
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Re: Making a liquid culture with a mushroom cap [Re: where_did_Panda_go]
    #23474583 - 07/25/16 06:46 AM (7 years, 6 months ago)

Oh my. The cap itself is covered in bacteria and other sorts of shit that's dropping down into the bottle, + the mushroom is nowhere near enough to prevent shit from the air getting down there..

We use agar to start cultures so we can transfer away from contams, and then use the clean agar for LC or whatever


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InvisibleSupalemonhaze
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Re: Making a liquid culture with a mushroom cap [Re: Yerow]
    #23474670 - 07/25/16 07:42 AM (7 years, 6 months ago)

That thing is uber fucked. Possibly the funniest shit I've seen so far. :lolsy:


OP, you really need to do a lot of reading. But to answer your question, agar still contaminates but since it's a 2d surface, you can take a piece of clean mycelium and transfer that into another plate until you get a clean culture. You then proceed to cut the plate into wedges and drop them into grain jars. If you wanna do liquids, I suggest LI over LC for a begginer, it's easier and most definitely safer.

Even I have trouble keeping an LC clean and I can do agar pretty easily and with minimal contamination. Using a mushroom cap as a lid in an open air environment is about the worst thing you can do, not to mention the funniest. Trash that thing before it gives you aids.


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Invisiblewhere_did_Panda_go
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Re: Making a liquid culture with a mushroom cap [Re: Supalemonhaze]
    #23474806 - 07/25/16 08:53 AM (7 years, 6 months ago)

Nah I want to see what happens, the world needs more love and aids anyway. Plus I can't throw it away, it's staring at me not to do it...



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Invisiblemupetmower
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Re: Making a liquid culture with a mushroom cap [Re: where_did_Panda_go]
    #23474811 - 07/25/16 08:55 AM (7 years, 6 months ago)

this just made my day :lol:


--------------------
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-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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OfflineBobabouy
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Re: Making a liquid culture with a mushroom cap [Re: where_did_Panda_go]
    #23474816 - 07/25/16 08:57 AM (7 years, 6 months ago)

Can never have enough aids. As long as it's in Africa.


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Invisibler.lutece
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Re: Making a liquid culture with a mushroom cap [Re: Bobabouy]
    #23474827 - 07/25/16 09:00 AM (7 years, 6 months ago)

God, it literally has a face.


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But one does not undertake an experiment knowing one HAS failed.


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InvisibleMunchauzen
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Re: Making a liquid culture with a mushroom cap [Re: where_did_Panda_go]
    #23474833 - 07/25/16 09:02 AM (7 years, 6 months ago)

Quote:

where_did_Panda_go said:
If spores are never sterile, how would it be able to not contaminate on agar? I read somewhere in recent posts agar gives spores more favorable environment, but do I really also need to add sugar and starch to an agar mixture used for desserts? Sugar no problem, but do I need to boil a potato to get starch?

I assumed that the mushroom cap is covering my bottle, so only spores can drop into the liquid culture. Or do you think this might had a better chance if I only used sterilized water? Most spore syringe only contain water and spores, would that not be a problem with contamination too?



On agar we can transfer away from contaminates to clean our culture up. A few generations of transfers and ur culture is squeaky clean.


Edited by Munchauzen (07/25/16 09:03 AM)


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Invisibleh0ldthedoor
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Re: Making a liquid culture with a mushroom cap [Re: Bobabouy]
    #23474853 - 07/25/16 09:13 AM (7 years, 6 months ago)

Quote:

Bobabouy said:
Can never have enough aids. As long as it's in Africa.



:notyou:


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

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Offlinemrmazdarx9
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Re: Making a liquid culture with a mushroom cap [Re: where_did_Panda_go]
    #23474864 - 07/25/16 09:19 AM (7 years, 6 months ago)

Quote:

where_did_Panda_go said:
Nah I want to see what happens, the world needs more love and aids anyway. Plus I can't throw it away, it's staring at me not to do it...





Looks like a homemade real boob feeder for babies or something out of texas chainsaw massacre


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Edited by mrmazdarx9 (07/25/16 09:24 AM)


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InvisiblePastywhyteMDiscord
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Re: Making a liquid culture with a mushroom cap [Re: mrmazdarx9]
    #23474873 - 07/25/16 09:22 AM (7 years, 6 months ago)

:popcorn:


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Invisiblemupetmower
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Re: Making a liquid culture with a mushroom cap [Re: Pastywhyte]
    #23474880 - 07/25/16 09:24 AM (7 years, 6 months ago)

Quote:

Pastywhyte said:
:popcorn:






damn you, nice ninja edit! was gonna add on the countless amounts of bacteria and mold spores to it =p


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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InvisiblePastywhyteMDiscord
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Re: Making a liquid culture with a mushroom cap [Re: mupetmower]
    #23474886 - 07/25/16 09:25 AM (7 years, 6 months ago)

:ninjawe:

Funnier this way. I actually want to see this play out lol.


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Offlinemrmazdarx9
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Re: Making a liquid culture with a mushroom cap [Re: Pastywhyte]
    #23474899 - 07/25/16 09:28 AM (7 years, 6 months ago)

You and muppetmower need to save your powers till you see my awesome agar attempt


--------------------
COCA GROWERS come here and share your knowledge
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Invisiblemupetmower
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Re: Making a liquid culture with a mushroom cap [Re: mrmazdarx9]
    #23474910 - 07/25/16 09:31 AM (7 years, 6 months ago)

i have no powers =\

but you'll do fine, man. you will get the hang of it. if i can manage to get some healthy plates from transfers, then you can too. anyone can.


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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InvisiblePastywhyteMDiscord
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Re: Making a liquid culture with a mushroom cap [Re: mupetmower]
    #23474923 - 07/25/16 09:36 AM (7 years, 6 months ago)

Yeah agar is easier than it sounds. Hardest part is just taking the plunge. Then once your doing it it get easier every time. Practice and muscle memory are 90% of the process.

There are no powers. I'm not a pro. I just muddle along like everyone else.


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Invisiblemupetmower
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Re: Making a liquid culture with a mushroom cap [Re: Pastywhyte]
    #23474926 - 07/25/16 09:37 AM (7 years, 6 months ago)

well... that's not true. you sir, are very much a pro. and we all learn from you every day. just think you should know that.

but enough stroking =p


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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InvisibleMad Season
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Re: Making a liquid culture with a mushroom cap [Re: where_did_Panda_go]
    #23474935 - 07/25/16 09:40 AM (7 years, 6 months ago)

Quote:

where_did_Panda_go said:
Nah I want to see what happens, the world needs more love and aids anyway. Plus I can't throw it away, it's staring at me not to do it...





:rofl: it's your grow man :P. I hope you got some spore prints going as well lol


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