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Invisibleuncle_rico
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agar work: cannabis
    #23471822 - 07/24/16 09:55 AM (7 years, 7 months ago)

Recently, a friend who is interested and involved in cannabis growing asked me to check around this site re.: agar work in cannabis propagation.

From what I understand, a piece of leaf (explant) is placed on agar, is grown, rooted and is eventially transplanted.  ?? really?

Is there a good tek around?

Can we use the SAB, petri dishes, agar that I have for mycology?

sorry, if I sound like a kook / noob.  I am.  it would be nice if someone could point me in an appropriate direction.

thanks guys.


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Invisiblemodern.shaman
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Re: agar work: cannabis [Re: uncle_rico] * 2
    #23471843 - 07/24/16 10:05 AM (7 years, 7 months ago)

You will need nutrients for the agar but yes you can use many of the skills you use for mycology for tissue culture.

You will also need to surface sterilize the leaf/stem(explant) and possible use some pgh although not necessary depending on what you want to do. Most plants will root without pgh although you might need bap since you will likely want to multiple the material before rooting.

Look at Plants from Test Tubes book which can be found online. https://www.omnisterra.com/botany/cp/slides/tc/tc.htm give a basic run thru on what you will need/do and provides a more diy approach to tissue culture.


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Invisibleuncle_rico
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Re: agar work: cannabis [Re: modern.shaman]
    #23472001 - 07/24/16 11:16 AM (7 years, 7 months ago)

Thank you, modern.shaman.

That was a very good link with great references.

Nice to know that the PC, SAB, petri dishes and general techniques can be used.

Is there a tek that is specific to cannabis?

Is anyone here doing tissue culturing?

thanks guys


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OfflinePOWAtrippinDiscord
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Re: agar work: cannabis [Re: uncle_rico]
    #23472351 - 07/24/16 01:22 PM (7 years, 7 months ago)

I would like to know your goal for using this type of propagation.


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Invisibleuncle_rico
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Re: agar work: cannabis [Re: POWAtrippin]
    #23472424 - 07/24/16 01:55 PM (7 years, 7 months ago)

I don't know exactly. I'll ask more directly.

What I remember from the conversation;  making sure that your mother plant is pathogen free and also, maybe,  long term storage of the genetics.

I'm not an herb guy.  My friend is way in to cannabis and brought up tissue culture and agar knowing that I have a PC and some sterile skiillz.

I'd like to help him. That's my goal.  I believe his goals are clean genetics and storage.  I'll check. I'm interested myself.


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OfflineBaronVonBud
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Re: agar work: cannabis [Re: uncle_rico]
    #23473574 - 07/24/16 08:39 PM (7 years, 7 months ago)

Long term storage of genetics without using lights and water to keep a mother would be great for breeders


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Invisiblethe_r3dz
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Re: agar work: cannabis [Re: BaronVonBud]
    #23473822 - 07/24/16 09:46 PM (7 years, 7 months ago)

I'm just gonna jump in here cuz this is an awesome subject.

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Invisiblepenker
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Re: agar work: cannabis [Re: the_r3dz]
    #23474581 - 07/25/16 06:45 AM (7 years, 7 months ago)

Quote:

the_r3dz said:
I'm just gonna jump in here cuz this is an awesome subject.




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Invisibledurian_2008
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Re: agar work: cannabis [Re: penker]
    #23479688 - 07/26/16 06:55 PM (7 years, 7 months ago)

It's called tissue culture or micropropagation.


Generally speaking, the plant is broken down into teeny pieces, with applications of rooting, growing, and branching hormone.

Your new test tube babies are said to be in vitro, take a very long time to reach hardy size, but thousands of clones are possible from a single leaf.

Pure chemicals can be sourced for very cheap, online, but need to be highly diluted for this delicate task.

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Invisibleuncle_rico
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Re: agar work: cannabis [Re: modern.shaman]
    #23482071 - 07/27/16 11:28 AM (7 years, 7 months ago)

Thanks guys.

My friend got the mentioned book and is also carefully going over the info in the link.

His response to what his goals are:  Breeding stock and virus free genetics.

Interested to hear if anyone is doing or has done microprpagation.


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OfflineIcon
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Re: agar work: cannabis [Re: uncle_rico]
    #23484069 - 07/27/16 10:18 PM (7 years, 7 months ago)

I was curious about agar for cannabis too until I tried aeroponic cloning. You can buy / build a cloner that can root and hold dozens of cuttings. I think the cloner would be better than an agar station.

I'm not sure how much agar can help clean a strain of viral infections. And for breeding stock, it sounds super slow to take a tiny piece of leaf and root from an agar plate, compared to snipping off a shoot from a growing plant.

I can see agar being useful if you have a strain you need to save and for some reason it isn't going to produce a cutting. But keeping a cycle of clones or a bonsai mother worth dozens of cuttings is pretty easy.

My cloner takes 1 cubic foot and holds 36 cuttings. Can just top and re-root to keep a whole collection of strains going. Just a thought.

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Invisibleweetsie
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Re: agar work: cannabis [Re: Icon]
    #23484341 - 07/28/16 01:21 AM (7 years, 7 months ago)

Unless I'm mistaken, you can't clone cannabis from a leaf with TC, you have to use nodes.


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Invisibledurian_2008
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Re: agar work: cannabis [Re: weetsie]
    #23486721 - 07/28/16 05:51 PM (7 years, 7 months ago)

It would be interesting to hear a scientific explanation for that, as an entire plant can supposedly be recreated from a single cell.

I'm not saying it isn't true, I just don't understand the reason why.

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Invisiblemodern.shaman
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Re: agar work: cannabis [Re: durian_2008]
    #23486825 - 07/28/16 06:18 PM (7 years, 7 months ago)

Totipotency

I guess it is similar to stem cells... they have all the data but have yet to differentiate to form anything? I don't have a complete understanding of it but that's the idea


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Invisibledurian_2008
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Re: agar work: cannabis [Re: modern.shaman]
    #23486884 - 07/28/16 06:27 PM (7 years, 7 months ago)

I get it. But, why should totipotent cells make a plant which is different from the parent.

Quote:

weetsie said:
Unless I'm mistaken, you can't clone cannabis from a leaf with TC, you have to use nodes.




:shrug: idk :strokebeard:

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Invisibleweetsie
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Re: agar work: cannabis [Re: durian_2008]
    #23487626 - 07/28/16 10:12 PM (7 years, 7 months ago)

I looked into TC when I got my flowhood for mushrooms as I had all the equipment needed minus some hormones and baby bottles, never actually got round to trying it.

I was mainly interested in orchids, though remember reading something about cannabis at one point where it mentioned clones from leafs not being possible.


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OfflineLSoares
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Re: agar work: cannabis [Re: uncle_rico]
    #23488147 - 07/29/16 03:04 AM (7 years, 7 months ago)

Quote:

durian_2008 said:
I get it. But, why should totipotent cells make a plant which is different from the parent.



Theoretically, plants derived from a single, de-differentiated cell will carry the replicating mistakes that happened when that cell was created. Very unlikely to have any significant meaning, IMHO.

Quote:

uncle_rico said:
His response to what his goals are:  Breeding stock and virus free genetics.



This is a good point: still theoretically, a fast growing mass of cells (the callus) can "outrun" a virus infection and virus free plants can thus be produced.


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