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OfflineShroomsh
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Inoculating again after a week.
    #23473363 - 07/24/16 07:40 PM (7 years, 6 months ago)

I mean it's sterile right?  It's been pasteurized.  Is there any reason to not inoculate a second time in brf jars that are suspected to have not been gifted spores?  Only difference would be 1st inoc was b+ 2nd would be golden teacher.


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InvisibleMoabfighter
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Re: Inoculating again after a week. [Re: Shroomsh]
    #23473427 - 07/24/16 08:04 PM (7 years, 6 months ago)

Wtf don't do that


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OfflineBaronVonBud
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Re: Inoculating again after a week. [Re: Moabfighter]
    #23473457 - 07/24/16 08:14 PM (7 years, 6 months ago)

You can, I've read a lot of post about mixing "strains" but it's only been a week. Why not wait 1-2 more and not risk adding anything bad.cakes take 7-8 days to show life but that life may be deep


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OfflineBaronVonBud
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Re: Inoculating again after a week. [Re: BaronVonBud]
    #23473464 - 07/24/16 08:15 PM (7 years, 6 months ago)

GT is the shit btw I love it


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OfflineDark.Mayan
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Re: Inoculating again after a week. [Re: BaronVonBud]
    #23473758 - 07/24/16 09:30 PM (7 years, 6 months ago)

Quote:

BaronVonBud said:
You can, I've read a lot of post about mixing "strains" but it's only been a week. Why not wait 1-2 more and not risk adding anything bad.cakes take 7-8 days to show life but that life may be deep




Patience - I heard is the key to harvesting.  I thought my cakes were robbed of their gift of spores, but I'm starting to see webs.  It's ultimately your call - good luck


--------------------
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InvisibleLocN9ne
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Re: Inoculating again after a week. [Re: Dark.Mayan]
    #23473814 - 07/24/16 09:44 PM (7 years, 6 months ago)

Just wait it out, or start a new set of cakes... Never re-inoculate anything.


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OfflineShroomsh
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Re: Inoculating again after a week. [Re: LocN9ne]
    #23478474 - 07/26/16 11:45 AM (7 years, 6 months ago)

Yeah thanks guys.  I'm waiting just a bit longer.  I'm only even considering re-inoculating because I stretched one 9ml syringe across 14 jars.  I'm just afraid some of them may not have gotten some spores seeing as the syringe was super clumpy.  I have a few extras so it wouldn't be a big deal to me.  I also have nearly no verm left and I'm not looking to go spend more money to start more cakes, so I don't want to waste what I already spent effort and time on you know? 

We'll see how they look at around thursday this week and if some still show no signs, then we'll experiment with re inoculation! 

Unless someone has a really good reason why I shouldn't inoculate a second time...


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Invisiblefilthyknees
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Re: Inoculating again after a week. [Re: Shroomsh]
    #23478485 - 07/26/16 11:49 AM (7 years, 6 months ago)

It's just the right way to do things.

Go ahead, reinnoc and fail; then you will have a good reason to do it right the next time.


--------------------
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InvisibleMad Season
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Re: Inoculating again after a week. [Re: Shroomsh]
    #23478489 - 07/26/16 11:52 AM (7 years, 6 months ago)

I guarantee they all got spores dude. 1 drop has spores in it even if they look like they clumped up. Shaking it only increases the amount of spores per drop.

If they don't germinate, there's obviously something going on, and don't just re-inoc. Make new jars if you want to re-inoc, it's like 10 cents a jar in materials anyways.


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OfflineShroomsh
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Re: Inoculating again after a week. [Re: filthyknees]
    #23478507 - 07/26/16 12:01 PM (7 years, 6 months ago)

Oh yeah?  And what will you have to say when I reinoc and it's all successful? 

Don't be shy now, let's not always do the same thing now.


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InvisibleMoabfighter
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Re: Inoculating again after a week. [Re: Shroomsh]
    #23478511 - 07/26/16 12:03 PM (7 years, 6 months ago)

Lol reinnoc you fuck.


No one. I repeat NO ONE intentionally shoots two different strains into one jar


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OfflineShroomsh
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Re: Inoculating again after a week. [Re: Moabfighter]
    #23478527 - 07/26/16 12:09 PM (7 years, 6 months ago)

I fail to see what is so scary about two strains in a jar.  It's still a cubensis!  What's really the worst that could happen?  Other than contams obviously. 

I think I might do it just to make you all mad


Edited by Shroomsh (07/26/16 12:09 PM)


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Offlinemrmazdarx9
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Re: Inoculating again after a week. [Re: Shroomsh]
    #23478535 - 07/26/16 12:13 PM (7 years, 6 months ago)

Quote:

Shroomsh said:
Oh yeah?  And what will you have to say when I reinoc and it's all successful? 

Don't be shy now, let's not always do the same thing now.



I tried that and got contams one the one jar I did it to on my first attempt, the dry verm barrier catches contams chances are even with a sterile needle you'll still pick up contams pushing through the already active verm barrier

Edit: thought you were doing Pf cakes


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Edited by mrmazdarx9 (07/26/16 12:14 PM)


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OfflineShroomsh
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Re: Inoculating again after a week. [Re: mrmazdarx9]
    #23478542 - 07/26/16 12:15 PM (7 years, 6 months ago)

Quote:

mrmazdarx9 said:
Quote:

Shroomsh said:
Oh yeah?  And what will you have to say when I reinoc and it's all successful? 

Don't be shy now, let's not always do the same thing now.



I tried that and got contams one the one jar I did it to on my first attempt, the dry verm barrier catches contams chances are even with a sterile needle you'll still pick up contams pushing through the already active verm barrier

Edit: thought you were doing Pf cakes




Mmm, this was really the biggest and only obstacle I foresaw as well.  Think it's too risky to sterilize a spoon and scoop some verm outta the way?


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Offlinemrmazdarx9
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Re: Inoculating again after a week. [Re: Shroomsh]
    #23478552 - 07/26/16 12:19 PM (7 years, 6 months ago)

Quote:

Shroomsh said:
Quote:

mrmazdarx9 said:
Quote:

Shroomsh said:
Oh yeah?  And what will you have to say when I reinoc and it's all successful? 

Don't be shy now, let's not always do the same thing now.



I tried that and got contams one the one jar I did it to on my first attempt, the dry verm barrier catches contams chances are even with a sterile needle you'll still pick up contams pushing through the already active verm barrier

Edit: thought you were doing Pf cakes




Mmm, this was really the biggest and only obstacle I foresaw as well.  Think it's too risky to sterilize a spoon and scoop some verm outta the way?



I'd wait it out after my experience but at the end of the day there your babies its down to you what you think is best :thumbup:


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Invisibleh0ldthedoor
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Re: Inoculating again after a week. [Re: Shroomsh]
    #23478565 - 07/26/16 12:26 PM (7 years, 6 months ago)

Quote:

Shroomsh said:
Think it's too risky to sterilize a spoon and scoop some verm outta the way?




Wouldn't this involve opening the jar(s)? From what I've read it seems like a bad idea.


--------------------
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OfflineShroomsh
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Re: Inoculating again after a week. [Re: mrmazdarx9]
    #23478578 - 07/26/16 12:32 PM (7 years, 6 months ago)

I'll wait a tad longer and if any show no signs of growth then we'll get jiggy with it. 

Hodor, yes it would involve some opening, but what I would probably do is just puncture a small hole through both layers of foil and try to work with that and then add a 3rd layer to seal it again.


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Invisiblefilthyknees
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Re: Inoculating again after a week. [Re: Shroomsh]
    #23478851 - 07/26/16 01:53 PM (7 years, 6 months ago)

Quote:

Shroomsh said:
Oh yeah?  And what will you have to say when I reinoc and it's all successful? 




I'd say: why are you wasting your time on cakes when grain spawn and bulk substrates will yield you more.

:rolleyes: now I'm gonna go pour 80 petris and do real work. Good luck.


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
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OfflineShroomsh
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Re: Inoculating again after a week. [Re: filthyknees]
    #23478927 - 07/26/16 02:17 PM (7 years, 6 months ago)

Quote:

filthyknees said:
Quote:

Shroomsh said:
Oh yeah?  And what will you have to say when I reinoc and it's all successful? 




I'd say: why are you wasting your time on cakes when grain spawn and bulk substrates will yield you more.

:rolleyes: now I'm gonna go pour 80 petris and do real work. Good luck.




Because this is only my 2nd grow and I'm just experimenting in various ways.  I'm interested in using different teks but just haven't gotten to those parts yet.  Once these cakes I have right now become colonized a partial amount of them will be transferred to a monotub that I'm gonna try out. 

Have fun with your cultures!  That's next on my list to learn.


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OfflineBaronVonBud
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Re: Inoculating again after a week. [Re: Shroomsh]
    #23478970 - 07/26/16 02:30 PM (7 years, 6 months ago)

When you say that it was real clumpy does that mean you didn't shake it up?


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Offlinemrmazdarx9
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Re: Inoculating again after a week. [Re: BaronVonBud]
    #23478987 - 07/26/16 02:35 PM (7 years, 6 months ago)

I think its Pf I may be wrong


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OfflineEywa_devotee
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Re: Inoculating again after a week. [Re: Shroomsh]
    #23478992 - 07/26/16 02:37 PM (7 years, 6 months ago)

PC and wait until they are cold and try again OR wait at least a couple weeks old. If no growth is seen at the two week mark chuck'em or set aside and see if anything happens- it's your call. Start over with new jars and PC them a little longer and make sure they cool to room temp before knocking them up next time.

The way i make mine is a few tablespoons of vermiculite on the bottom, about 1/4 inch or so. In the middle is the well hydrated birdseed, oats or BRF+verm mixture, and at the top is a few tablespoons of dry vermiculite. This layer is just enough to thoroughly cover the grains in the middle, 1/8 to 1/4 inch thick. Tyvek homewrap is used as the GE membrane at the top. PC at 15 PSI for 90 minutes for grains and 30 minutes for brown rice or BRF/verm. Generally wait until the next day to inoculate them because it takes so long to cool. IME, it is OK to give them a second PC treatment ONCE if after a week or so no growth is seen. If they fail the second time chuck'em and start with fresh grains.

In mycology, patience is a virtue. Better to take your time and do it right than hurry and have an epic fail.


--------------------
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InvisibleMad Season
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Re: Inoculating again after a week. [Re: Mad Season]
    #23479042 - 07/26/16 02:54 PM (7 years, 6 months ago)

Quote:

Mad Season said:
I guarantee they all got spores dude. 1 drop has spores in it even if they look like they clumped up. Shaking it only increases the amount of spores per drop.

If they don't germinate, there's obviously something going on, and don't just re-inoc. Make new jars if you want to re-inoc, it's like 10 cents a jar in materials anyways.



I was like what the fuck why didn't he even acknowledge this. Then I checked his ratings and saw I gave him a 0 for ignoring me :rofl:


--------------------
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Re: Inoculating again after a week. [Re: Eywa_devotee]
    #23479044 - 07/26/16 02:55 PM (7 years, 6 months ago)

Shake the spores up right before you flame the needle. They shouldn't be all clumped together when u use it


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Offlinemrmazdarx9
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Re: Inoculating again after a week. [Re: Mad Season]
    #23479106 - 07/26/16 03:15 PM (7 years, 6 months ago)

Quote:

Mad Season said:
Quote:

Mad Season said:
I guarantee they all got spores dude. 1 drop has spores in it even if they look like they clumped up. Shaking it only increases the amount of spores per drop.

If they don't germinate, there's obviously something going on, and don't just re-inoc. Make new jars if you want to re-inoc, it's like 10 cents a jar in materials anyways.



I was like what the fuck why didn't he even acknowledge this. Then I checked his ratings and saw I gave him a 0 for ignoring me :rofl:



Never ignore mad season :whip:


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OfflineBaronVonBud
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Re: Inoculating again after a week. [Re: mrmazdarx9]
    #23479130 - 07/26/16 03:27 PM (7 years, 6 months ago)

If it's all still clumped up who's to say those few random spores that get used are alive


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InvisibleMad Season
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Re: Inoculating again after a week. [Re: BaronVonBud]
    #23479145 - 07/26/16 03:35 PM (7 years, 6 months ago)

Few random spores? Really? Lol!!

If there's even visible specks, not even clumps, 1 drop should EASILY have 500000+ spores

^ that speck is 10 million spores. A speck. Literally smaller than a bread crumb.


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Re: Inoculating again after a week. [Re: Shroomsh]
    #23479175 - 07/26/16 03:47 PM (7 years, 6 months ago)

Quote:

Shroomsh said:
I'll wait a tad longer and if any show no signs of growth then we'll get jiggy with it. 

Hodor, yes it would involve some opening, but what I would probably do is just puncture a small hole through both layers of foil and try to work with that and then add a 3rd layer to seal it again.




What tek are you using?  If it's cakes (PF Tek), why is there foil involved?


--------------------
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Offlinemrmazdarx9
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Re: Inoculating again after a week. [Re: KenInVic]
    #23479207 - 07/26/16 04:05 PM (7 years, 6 months ago)

Quote:

KenInVic said:
Quote:

Shroomsh said:
I'll wait a tad longer and if any show no signs of growth then we'll get jiggy with it. 

Hodor, yes it would involve some opening, but what I would probably do is just puncture a small hole through both layers of foil and try to work with that and then add a 3rd layer to seal it again.




What tek are you using?  If it's cakes (PF Tek), why is there foil involved?



Probably no lid


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OfflineBaronVonBud
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Re: Inoculating again after a week. [Re: Mad Season]
    #23479261 - 07/26/16 04:25 PM (7 years, 6 months ago)

How many would it be if not visible. I can see the spec in that thread has spores because it's dark. I've seen clear areas in unshaken syringes .How old is the syringe too


Edited by BaronVonBud (07/26/16 04:32 PM)


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OfflineBaronVonBud
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Re: Inoculating again after a week. [Re: BaronVonBud]
    #23479269 - 07/26/16 04:28 PM (7 years, 6 months ago)

I'm pretty sure my empty LC is from not shaking on the first jar
He's for sure crazy on wanting to put more after a week but why on earth would not shaking be encouraged


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Edited by BaronVonBud (07/26/16 04:34 PM)


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InvisibleMad Season
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Re: Inoculating again after a week. [Re: BaronVonBud]
    #23479280 - 07/26/16 04:33 PM (7 years, 6 months ago)

Doubt it. Germination can take a long time. I mean I guess it's possible, just soo unlikely that there's no spores when you use a syringe. Even without shaking


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Re: Inoculating again after a week. [Re: Shroomsh]
    #23479283 - 07/26/16 04:34 PM (7 years, 6 months ago)

Quote:

Shroomsh said:
Quote:

mrmazdarx9 said:
Quote:

Shroomsh said:
Oh yeah?  And what will you have to say when I reinoc and it's all successful? 

Don't be shy now, let's not always do the same thing now.



I tried that and got contams one the one jar I did it to on my first attempt, the dry verm barrier catches contams chances are even with a sterile needle you'll still pick up contams pushing through the already active verm barrier

Edit: thought you were doing Pf cakes




Mmm, this was really the biggest and only obstacle I foresaw as well.  Think it's too risky to sterilize a spoon and scoop some verm outta the way?




Exactly. Don't listen to all these people that grow regularly, just follow your gut. You know better than all of them.
If you want to get colonization almost instantly, take off the lid, scoop some of the verm out of the way, dump spores in and crumple up some foil and throw it on top. Repeat for all your stalled jars. They didn't germinate only because 50,000+ spores isn't enough sometimes. Foil is an excellent filter--I wonder why more people don't use it as one.

More is always better. Why? Because 'Merca.


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OfflineBaronVonBud
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Re: Inoculating again after a week. [Re: Mad Season]
    #23479288 - 07/26/16 04:36 PM (7 years, 6 months ago)

I did two LC jars with the same syringe before realizing that it's old news to do that and I have one that's full of cum looking growth and one that's crystal clear and I forgot to shake on that first one both are 8-9 days


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Re: Inoculating again after a week. [Re: BaronVonBud]
    #23479300 - 07/26/16 04:41 PM (7 years, 6 months ago)

Idk what you're trying to tell me lol. That you have growth in the one you shook before inoc after 8-9 days? Let's hope it's cubensis lol


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Re: Inoculating again after a week. [Re: Mad Season]
    #23479303 - 07/26/16 04:42 PM (7 years, 6 months ago)

Ya, I'm pretty sure not shaking caused the empty one
Used cybers LC tek and 8-9 days showing growth is normal for that, it says 7-10 and it's good to go

Did u mean hope it's not contaminated as well as myc ?


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Edited by BaronVonBud (07/26/16 04:44 PM)


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Re: Inoculating again after a week. [Re: BaronVonBud]
    #23479316 - 07/26/16 04:53 PM (7 years, 6 months ago)

No I meant let's hope it's cubensis, but yeah let's also hope what you said too. Cubensis IME takes 3-21 days to germinate, and I've even seen shit take 2 months to germinate.

LC almost everything will look mucousy in a LC. Agar is the way to tell if it's clean. That's why you gotta test your LC cleanliness on agar. Oh and even then, fuckin contaminations can be hidden on agar


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Re: Inoculating again after a week. [Re: Mad Season]
    #23479335 - 07/26/16 04:59 PM (7 years, 6 months ago)

I'm about to get into agar after figuring out I needed to sort results by date lol
I haven't been involved in the hobby in a long time but the only contamination I've had that was obvious and prevented good smelling results that gave fruit (I hear all technically has something bad) was when I tried a really low spawn to bulk ratio with pre-made overpriced bulk

I'm on my 4-5 run at rye for bulk and my first go at cakes and both now and always have had growth within 10 days


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Edited by BaronVonBud (07/26/16 05:02 PM)


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OfflineBaronVonBud
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Re: Inoculating again after a week. [Re: BaronVonBud]
    #23479374 - 07/26/16 05:12 PM (7 years, 6 months ago)



Day 8 no dry layer since its PC'd and has Tyvek and RTV ports


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Edited by BaronVonBud (07/26/16 05:14 PM)


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Re: Inoculating again after a week. [Re: BaronVonBud]
    #23480716 - 07/26/16 11:24 PM (7 years, 6 months ago)

This is why I gave OP a little bitch rating.


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Re: Inoculating again after a week. [Re: BaronVonBud]
    #23482385 - 07/27/16 01:33 PM (7 years, 6 months ago)

No, I shook the fuck outta that syringe and stop once I saw it leaking the solution all over the bag.  I got the spores as broken up as I could without losing it all.


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Re: Inoculating again after a week. [Re: Shroomsh]
    #23482948 - 07/27/16 04:51 PM (7 years, 6 months ago)

We're the spores from a good source ?


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Re: Inoculating again after a week. [Re: BaronVonBud]
    #23484159 - 07/27/16 11:10 PM (7 years, 6 months ago)

Name one source that doesn't occasionally produce spores with issues?  As of now I don't know any.  In fact don't name them even if they do exist, board rules and all..


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Re: Inoculating again after a week. [Re: Inocuole]
    #23484633 - 07/28/16 06:01 AM (7 years, 6 months ago)

Quote:

Inocuole said:
Name one source that doesn't occasionally produce spores with issues?  As of now I don't know any.  In fact don't name them even if they do exist, board rules and all..



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OfflineMy Boy
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Re: Inoculating again after a week. [Re: mrmazdarx9]
    #23484683 - 07/28/16 06:41 AM (7 years, 6 months ago)

where on the jar did you inoculate and what is your incubation temp?


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