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Offlinemrmazdarx9
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Re: Inoculating again after a week. [Re: BaronVonBud]
    #23478987 - 07/26/16 02:35 PM (7 years, 6 months ago)

I think its Pf I may be wrong


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OfflineEywa_devotee
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Re: Inoculating again after a week. [Re: Shroomsh]
    #23478992 - 07/26/16 02:37 PM (7 years, 6 months ago)

PC and wait until they are cold and try again OR wait at least a couple weeks old. If no growth is seen at the two week mark chuck'em or set aside and see if anything happens- it's your call. Start over with new jars and PC them a little longer and make sure they cool to room temp before knocking them up next time.

The way i make mine is a few tablespoons of vermiculite on the bottom, about 1/4 inch or so. In the middle is the well hydrated birdseed, oats or BRF+verm mixture, and at the top is a few tablespoons of dry vermiculite. This layer is just enough to thoroughly cover the grains in the middle, 1/8 to 1/4 inch thick. Tyvek homewrap is used as the GE membrane at the top. PC at 15 PSI for 90 minutes for grains and 30 minutes for brown rice or BRF/verm. Generally wait until the next day to inoculate them because it takes so long to cool. IME, it is OK to give them a second PC treatment ONCE if after a week or so no growth is seen. If they fail the second time chuck'em and start with fresh grains.

In mycology, patience is a virtue. Better to take your time and do it right than hurry and have an epic fail.


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InvisibleMad Season
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Re: Inoculating again after a week. [Re: Mad Season]
    #23479042 - 07/26/16 02:54 PM (7 years, 6 months ago)

Quote:

Mad Season said:
I guarantee they all got spores dude. 1 drop has spores in it even if they look like they clumped up. Shaking it only increases the amount of spores per drop.

If they don't germinate, there's obviously something going on, and don't just re-inoc. Make new jars if you want to re-inoc, it's like 10 cents a jar in materials anyways.



I was like what the fuck why didn't he even acknowledge this. Then I checked his ratings and saw I gave him a 0 for ignoring me :rofl:


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OfflineBaronVonBud
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Re: Inoculating again after a week. [Re: Eywa_devotee]
    #23479044 - 07/26/16 02:55 PM (7 years, 6 months ago)

Shake the spores up right before you flame the needle. They shouldn't be all clumped together when u use it


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Offlinemrmazdarx9
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Re: Inoculating again after a week. [Re: Mad Season]
    #23479106 - 07/26/16 03:15 PM (7 years, 6 months ago)

Quote:

Mad Season said:
Quote:

Mad Season said:
I guarantee they all got spores dude. 1 drop has spores in it even if they look like they clumped up. Shaking it only increases the amount of spores per drop.

If they don't germinate, there's obviously something going on, and don't just re-inoc. Make new jars if you want to re-inoc, it's like 10 cents a jar in materials anyways.



I was like what the fuck why didn't he even acknowledge this. Then I checked his ratings and saw I gave him a 0 for ignoring me :rofl:



Never ignore mad season :whip:


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OfflineBaronVonBud
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Re: Inoculating again after a week. [Re: mrmazdarx9]
    #23479130 - 07/26/16 03:27 PM (7 years, 6 months ago)

If it's all still clumped up who's to say those few random spores that get used are alive


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InvisibleMad Season
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Re: Inoculating again after a week. [Re: BaronVonBud]
    #23479145 - 07/26/16 03:35 PM (7 years, 6 months ago)

Few random spores? Really? Lol!!

If there's even visible specks, not even clumps, 1 drop should EASILY have 500000+ spores

^ that speck is 10 million spores. A speck. Literally smaller than a bread crumb.


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InvisibleKenInVic
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Re: Inoculating again after a week. [Re: Shroomsh]
    #23479175 - 07/26/16 03:47 PM (7 years, 6 months ago)

Quote:

Shroomsh said:
I'll wait a tad longer and if any show no signs of growth then we'll get jiggy with it. 

Hodor, yes it would involve some opening, but what I would probably do is just puncture a small hole through both layers of foil and try to work with that and then add a 3rd layer to seal it again.




What tek are you using?  If it's cakes (PF Tek), why is there foil involved?


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Offlinemrmazdarx9
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Re: Inoculating again after a week. [Re: KenInVic]
    #23479207 - 07/26/16 04:05 PM (7 years, 6 months ago)

Quote:

KenInVic said:
Quote:

Shroomsh said:
I'll wait a tad longer and if any show no signs of growth then we'll get jiggy with it. 

Hodor, yes it would involve some opening, but what I would probably do is just puncture a small hole through both layers of foil and try to work with that and then add a 3rd layer to seal it again.




What tek are you using?  If it's cakes (PF Tek), why is there foil involved?



Probably no lid


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OfflineBaronVonBud
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Re: Inoculating again after a week. [Re: Mad Season]
    #23479261 - 07/26/16 04:25 PM (7 years, 6 months ago)

How many would it be if not visible. I can see the spec in that thread has spores because it's dark. I've seen clear areas in unshaken syringes .How old is the syringe too


Edited by BaronVonBud (07/26/16 04:32 PM)


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OfflineBaronVonBud
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Re: Inoculating again after a week. [Re: BaronVonBud]
    #23479269 - 07/26/16 04:28 PM (7 years, 6 months ago)

I'm pretty sure my empty LC is from not shaking on the first jar
He's for sure crazy on wanting to put more after a week but why on earth would not shaking be encouraged


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Edited by BaronVonBud (07/26/16 04:34 PM)


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InvisibleMad Season
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Re: Inoculating again after a week. [Re: BaronVonBud]
    #23479280 - 07/26/16 04:33 PM (7 years, 6 months ago)

Doubt it. Germination can take a long time. I mean I guess it's possible, just soo unlikely that there's no spores when you use a syringe. Even without shaking


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Invisibledankington
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Re: Inoculating again after a week. [Re: Shroomsh]
    #23479283 - 07/26/16 04:34 PM (7 years, 6 months ago)

Quote:

Shroomsh said:
Quote:

mrmazdarx9 said:
Quote:

Shroomsh said:
Oh yeah?  And what will you have to say when I reinoc and it's all successful? 

Don't be shy now, let's not always do the same thing now.



I tried that and got contams one the one jar I did it to on my first attempt, the dry verm barrier catches contams chances are even with a sterile needle you'll still pick up contams pushing through the already active verm barrier

Edit: thought you were doing Pf cakes




Mmm, this was really the biggest and only obstacle I foresaw as well.  Think it's too risky to sterilize a spoon and scoop some verm outta the way?




Exactly. Don't listen to all these people that grow regularly, just follow your gut. You know better than all of them.
If you want to get colonization almost instantly, take off the lid, scoop some of the verm out of the way, dump spores in and crumple up some foil and throw it on top. Repeat for all your stalled jars. They didn't germinate only because 50,000+ spores isn't enough sometimes. Foil is an excellent filter--I wonder why more people don't use it as one.

More is always better. Why? Because 'Merca.


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OfflineBaronVonBud
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Re: Inoculating again after a week. [Re: Mad Season]
    #23479288 - 07/26/16 04:36 PM (7 years, 6 months ago)

I did two LC jars with the same syringe before realizing that it's old news to do that and I have one that's full of cum looking growth and one that's crystal clear and I forgot to shake on that first one both are 8-9 days


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InvisibleMad Season
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Re: Inoculating again after a week. [Re: BaronVonBud]
    #23479300 - 07/26/16 04:41 PM (7 years, 6 months ago)

Idk what you're trying to tell me lol. That you have growth in the one you shook before inoc after 8-9 days? Let's hope it's cubensis lol


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OfflineBaronVonBud
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Re: Inoculating again after a week. [Re: Mad Season]
    #23479303 - 07/26/16 04:42 PM (7 years, 6 months ago)

Ya, I'm pretty sure not shaking caused the empty one
Used cybers LC tek and 8-9 days showing growth is normal for that, it says 7-10 and it's good to go

Did u mean hope it's not contaminated as well as myc ?


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Edited by BaronVonBud (07/26/16 04:44 PM)


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InvisibleMad Season
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Re: Inoculating again after a week. [Re: BaronVonBud]
    #23479316 - 07/26/16 04:53 PM (7 years, 6 months ago)

No I meant let's hope it's cubensis, but yeah let's also hope what you said too. Cubensis IME takes 3-21 days to germinate, and I've even seen shit take 2 months to germinate.

LC almost everything will look mucousy in a LC. Agar is the way to tell if it's clean. That's why you gotta test your LC cleanliness on agar. Oh and even then, fuckin contaminations can be hidden on agar


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OfflineBaronVonBud
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Re: Inoculating again after a week. [Re: Mad Season]
    #23479335 - 07/26/16 04:59 PM (7 years, 6 months ago)

I'm about to get into agar after figuring out I needed to sort results by date lol
I haven't been involved in the hobby in a long time but the only contamination I've had that was obvious and prevented good smelling results that gave fruit (I hear all technically has something bad) was when I tried a really low spawn to bulk ratio with pre-made overpriced bulk

I'm on my 4-5 run at rye for bulk and my first go at cakes and both now and always have had growth within 10 days


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Edited by BaronVonBud (07/26/16 05:02 PM)


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OfflineBaronVonBud
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Re: Inoculating again after a week. [Re: BaronVonBud]
    #23479374 - 07/26/16 05:12 PM (7 years, 6 months ago)



Day 8 no dry layer since its PC'd and has Tyvek and RTV ports


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Edited by BaronVonBud (07/26/16 05:14 PM)


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InvisibleInocuole
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Re: Inoculating again after a week. [Re: BaronVonBud]
    #23480716 - 07/26/16 11:24 PM (7 years, 6 months ago)

This is why I gave OP a little bitch rating.


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