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OfflineShroomsh
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Inoculating again after a week.
    #23473363 - 07/24/16 07:40 PM (7 years, 6 months ago)

I mean it's sterile right?  It's been pasteurized.  Is there any reason to not inoculate a second time in brf jars that are suspected to have not been gifted spores?  Only difference would be 1st inoc was b+ 2nd would be golden teacher.


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InvisibleMoabfighter
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Re: Inoculating again after a week. [Re: Shroomsh]
    #23473427 - 07/24/16 08:04 PM (7 years, 6 months ago)

Wtf don't do that


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OfflineBaronVonBud
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Re: Inoculating again after a week. [Re: Moabfighter]
    #23473457 - 07/24/16 08:14 PM (7 years, 6 months ago)

You can, I've read a lot of post about mixing "strains" but it's only been a week. Why not wait 1-2 more and not risk adding anything bad.cakes take 7-8 days to show life but that life may be deep


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OfflineBaronVonBud
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Re: Inoculating again after a week. [Re: BaronVonBud]
    #23473464 - 07/24/16 08:15 PM (7 years, 6 months ago)

GT is the shit btw I love it


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OfflineDark.Mayan
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Re: Inoculating again after a week. [Re: BaronVonBud]
    #23473758 - 07/24/16 09:30 PM (7 years, 6 months ago)

Quote:

BaronVonBud said:
You can, I've read a lot of post about mixing "strains" but it's only been a week. Why not wait 1-2 more and not risk adding anything bad.cakes take 7-8 days to show life but that life may be deep




Patience - I heard is the key to harvesting.  I thought my cakes were robbed of their gift of spores, but I'm starting to see webs.  It's ultimately your call - good luck


--------------------
It was from within this darkness that I found light - my light - yet again.  It was w/ the aid supplied from the medicine of ancients which rejuvenated my soul.  The love & gratitude had been within me all along - all that was needed, was a catalyst...


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InvisibleLocN9ne
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Re: Inoculating again after a week. [Re: Dark.Mayan]
    #23473814 - 07/24/16 09:44 PM (7 years, 6 months ago)

Just wait it out, or start a new set of cakes... Never re-inoculate anything.


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Q&A
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OfflineShroomsh
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Re: Inoculating again after a week. [Re: LocN9ne]
    #23478474 - 07/26/16 11:45 AM (7 years, 6 months ago)

Yeah thanks guys.  I'm waiting just a bit longer.  I'm only even considering re-inoculating because I stretched one 9ml syringe across 14 jars.  I'm just afraid some of them may not have gotten some spores seeing as the syringe was super clumpy.  I have a few extras so it wouldn't be a big deal to me.  I also have nearly no verm left and I'm not looking to go spend more money to start more cakes, so I don't want to waste what I already spent effort and time on you know? 

We'll see how they look at around thursday this week and if some still show no signs, then we'll experiment with re inoculation! 

Unless someone has a really good reason why I shouldn't inoculate a second time...


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Invisiblefilthyknees
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Re: Inoculating again after a week. [Re: Shroomsh]
    #23478485 - 07/26/16 11:49 AM (7 years, 6 months ago)

It's just the right way to do things.

Go ahead, reinnoc and fail; then you will have a good reason to do it right the next time.


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
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InvisibleMad Season
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Re: Inoculating again after a week. [Re: Shroomsh]
    #23478489 - 07/26/16 11:52 AM (7 years, 6 months ago)

I guarantee they all got spores dude. 1 drop has spores in it even if they look like they clumped up. Shaking it only increases the amount of spores per drop.

If they don't germinate, there's obviously something going on, and don't just re-inoc. Make new jars if you want to re-inoc, it's like 10 cents a jar in materials anyways.


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OfflineShroomsh
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Re: Inoculating again after a week. [Re: filthyknees]
    #23478507 - 07/26/16 12:01 PM (7 years, 6 months ago)

Oh yeah?  And what will you have to say when I reinoc and it's all successful? 

Don't be shy now, let's not always do the same thing now.


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InvisibleMoabfighter
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Re: Inoculating again after a week. [Re: Shroomsh]
    #23478511 - 07/26/16 12:03 PM (7 years, 6 months ago)

Lol reinnoc you fuck.


No one. I repeat NO ONE intentionally shoots two different strains into one jar


--------------------
KSSS And PE WBS.
 


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OfflineShroomsh
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Re: Inoculating again after a week. [Re: Moabfighter]
    #23478527 - 07/26/16 12:09 PM (7 years, 6 months ago)

I fail to see what is so scary about two strains in a jar.  It's still a cubensis!  What's really the worst that could happen?  Other than contams obviously. 

I think I might do it just to make you all mad


Edited by Shroomsh (07/26/16 12:09 PM)


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Offlinemrmazdarx9
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Re: Inoculating again after a week. [Re: Shroomsh]
    #23478535 - 07/26/16 12:13 PM (7 years, 6 months ago)

Quote:

Shroomsh said:
Oh yeah?  And what will you have to say when I reinoc and it's all successful? 

Don't be shy now, let's not always do the same thing now.



I tried that and got contams one the one jar I did it to on my first attempt, the dry verm barrier catches contams chances are even with a sterile needle you'll still pick up contams pushing through the already active verm barrier

Edit: thought you were doing Pf cakes


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Edited by mrmazdarx9 (07/26/16 12:14 PM)


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OfflineShroomsh
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Re: Inoculating again after a week. [Re: mrmazdarx9]
    #23478542 - 07/26/16 12:15 PM (7 years, 6 months ago)

Quote:

mrmazdarx9 said:
Quote:

Shroomsh said:
Oh yeah?  And what will you have to say when I reinoc and it's all successful? 

Don't be shy now, let's not always do the same thing now.



I tried that and got contams one the one jar I did it to on my first attempt, the dry verm barrier catches contams chances are even with a sterile needle you'll still pick up contams pushing through the already active verm barrier

Edit: thought you were doing Pf cakes




Mmm, this was really the biggest and only obstacle I foresaw as well.  Think it's too risky to sterilize a spoon and scoop some verm outta the way?


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Offlinemrmazdarx9
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Re: Inoculating again after a week. [Re: Shroomsh]
    #23478552 - 07/26/16 12:19 PM (7 years, 6 months ago)

Quote:

Shroomsh said:
Quote:

mrmazdarx9 said:
Quote:

Shroomsh said:
Oh yeah?  And what will you have to say when I reinoc and it's all successful? 

Don't be shy now, let's not always do the same thing now.



I tried that and got contams one the one jar I did it to on my first attempt, the dry verm barrier catches contams chances are even with a sterile needle you'll still pick up contams pushing through the already active verm barrier

Edit: thought you were doing Pf cakes




Mmm, this was really the biggest and only obstacle I foresaw as well.  Think it's too risky to sterilize a spoon and scoop some verm outta the way?



I'd wait it out after my experience but at the end of the day there your babies its down to you what you think is best :thumbup:


--------------------
COCA GROWERS come here and share your knowledge
COCA GROWERS UNITE
:penis:Click here for UK trades:penis:
:penis: need some supplies in the UK check Here or PM me:penis:
UK OTD uk members chat
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Right Here
If you use "SWIM" you should DROWN


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Invisibleh0ldthedoor
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Re: Inoculating again after a week. [Re: Shroomsh]
    #23478565 - 07/26/16 12:26 PM (7 years, 6 months ago)

Quote:

Shroomsh said:
Think it's too risky to sterilize a spoon and scoop some verm outta the way?




Wouldn't this involve opening the jar(s)? From what I've read it seems like a bad idea.


--------------------
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OfflineShroomsh
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Re: Inoculating again after a week. [Re: mrmazdarx9]
    #23478578 - 07/26/16 12:32 PM (7 years, 6 months ago)

I'll wait a tad longer and if any show no signs of growth then we'll get jiggy with it. 

Hodor, yes it would involve some opening, but what I would probably do is just puncture a small hole through both layers of foil and try to work with that and then add a 3rd layer to seal it again.


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Invisiblefilthyknees
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Re: Inoculating again after a week. [Re: Shroomsh]
    #23478851 - 07/26/16 01:53 PM (7 years, 6 months ago)

Quote:

Shroomsh said:
Oh yeah?  And what will you have to say when I reinoc and it's all successful? 




I'd say: why are you wasting your time on cakes when grain spawn and bulk substrates will yield you more.

:rolleyes: now I'm gonna go pour 80 petris and do real work. Good luck.


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night


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OfflineShroomsh
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Re: Inoculating again after a week. [Re: filthyknees]
    #23478927 - 07/26/16 02:17 PM (7 years, 6 months ago)

Quote:

filthyknees said:
Quote:

Shroomsh said:
Oh yeah?  And what will you have to say when I reinoc and it's all successful? 




I'd say: why are you wasting your time on cakes when grain spawn and bulk substrates will yield you more.

:rolleyes: now I'm gonna go pour 80 petris and do real work. Good luck.




Because this is only my 2nd grow and I'm just experimenting in various ways.  I'm interested in using different teks but just haven't gotten to those parts yet.  Once these cakes I have right now become colonized a partial amount of them will be transferred to a monotub that I'm gonna try out. 

Have fun with your cultures!  That's next on my list to learn.


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OfflineBaronVonBud
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Re: Inoculating again after a week. [Re: Shroomsh]
    #23478970 - 07/26/16 02:30 PM (7 years, 6 months ago)

When you say that it was real clumpy does that mean you didn't shake it up?


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