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Shroomsh
Mad King



Registered: 07/28/15
Posts: 121
Loc: Washington
Last seen: 7 years, 3 months
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Inoculating again after a week.
#23473363 - 07/24/16 07:40 PM (7 years, 6 months ago) |
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I mean it's sterile right? It's been pasteurized. Is there any reason to not inoculate a second time in brf jars that are suspected to have not been gifted spores? Only difference would be 1st inoc was b+ 2nd would be golden teacher.
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: Inoculating again after a week. [Re: Shroomsh]
#23473427 - 07/24/16 08:04 PM (7 years, 6 months ago) |
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Wtf don't do that
-------------------- KSSS And PE WBS.
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BaronVonBud
Stranger

Registered: 07/14/16
Posts: 143
Last seen: 7 years, 3 months
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Re: Inoculating again after a week. [Re: Moabfighter]
#23473457 - 07/24/16 08:14 PM (7 years, 6 months ago) |
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You can, I've read a lot of post about mixing "strains" but it's only been a week. Why not wait 1-2 more and not risk adding anything bad.cakes take 7-8 days to show life but that life may be deep
-------------------- <iframe src="//giphy.com/embed/Z7TvUbQHuFdBu" width="480" height="352" frameBorder="0" class="giphy-embed" allowFullScreen></iframe><p><a href="http://giphy.com/gifs/rock-flag-eagle-Z7TvUbQHuFdBu">via GIPHY</a></p>
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BaronVonBud
Stranger

Registered: 07/14/16
Posts: 143
Last seen: 7 years, 3 months
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Re: Inoculating again after a week. [Re: BaronVonBud]
#23473464 - 07/24/16 08:15 PM (7 years, 6 months ago) |
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GT is the shit btw I love it
-------------------- <iframe src="//giphy.com/embed/Z7TvUbQHuFdBu" width="480" height="352" frameBorder="0" class="giphy-embed" allowFullScreen></iframe><p><a href="http://giphy.com/gifs/rock-flag-eagle-Z7TvUbQHuFdBu">via GIPHY</a></p>
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Dark.Mayan
Welterweight divizion



Registered: 07/24/16
Posts: 21
Last seen: 7 years, 3 months
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Re: Inoculating again after a week. [Re: BaronVonBud]
#23473758 - 07/24/16 09:30 PM (7 years, 6 months ago) |
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Quote:
BaronVonBud said: You can, I've read a lot of post about mixing "strains" but it's only been a week. Why not wait 1-2 more and not risk adding anything bad.cakes take 7-8 days to show life but that life may be deep
Patience - I heard is the key to harvesting. I thought my cakes were robbed of their gift of spores, but I'm starting to see webs. It's ultimately your call - good luck
-------------------- It was from within this darkness that I found light - my light - yet again. It was w/ the aid supplied from the medicine of ancients which rejuvenated my soul. The love & gratitude had been within me all along - all that was needed, was a catalyst...
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: Inoculating again after a week. [Re: Dark.Mayan]
#23473814 - 07/24/16 09:44 PM (7 years, 6 months ago) |
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Just wait it out, or start a new set of cakes... Never re-inoculate anything.
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Q&A US vs. THEM The more I learn, the less I know.
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Shroomsh
Mad King



Registered: 07/28/15
Posts: 121
Loc: Washington
Last seen: 7 years, 3 months
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Re: Inoculating again after a week. [Re: LocN9ne]
#23478474 - 07/26/16 11:45 AM (7 years, 6 months ago) |
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Yeah thanks guys. I'm waiting just a bit longer. I'm only even considering re-inoculating because I stretched one 9ml syringe across 14 jars. I'm just afraid some of them may not have gotten some spores seeing as the syringe was super clumpy. I have a few extras so it wouldn't be a big deal to me. I also have nearly no verm left and I'm not looking to go spend more money to start more cakes, so I don't want to waste what I already spent effort and time on you know?
We'll see how they look at around thursday this week and if some still show no signs, then we'll experiment with re inoculation!
Unless someone has a really good reason why I shouldn't inoculate a second time...
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: Inoculating again after a week. [Re: Shroomsh]
#23478485 - 07/26/16 11:49 AM (7 years, 6 months ago) |
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It's just the right way to do things.
Go ahead, reinnoc and fail; then you will have a good reason to do it right the next time.
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Inoculating again after a week. [Re: Shroomsh]
#23478489 - 07/26/16 11:52 AM (7 years, 6 months ago) |
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I guarantee they all got spores dude. 1 drop has spores in it even if they look like they clumped up. Shaking it only increases the amount of spores per drop.
If they don't germinate, there's obviously something going on, and don't just re-inoc. Make new jars if you want to re-inoc, it's like 10 cents a jar in materials anyways.
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Shroomsh
Mad King



Registered: 07/28/15
Posts: 121
Loc: Washington
Last seen: 7 years, 3 months
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Re: Inoculating again after a week. [Re: filthyknees]
#23478507 - 07/26/16 12:01 PM (7 years, 6 months ago) |
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Oh yeah? And what will you have to say when I reinoc and it's all successful?
Don't be shy now, let's not always do the same thing now.
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: Inoculating again after a week. [Re: Shroomsh]
#23478511 - 07/26/16 12:03 PM (7 years, 6 months ago) |
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Lol reinnoc you fuck.
No one. I repeat NO ONE intentionally shoots two different strains into one jar
-------------------- KSSS And PE WBS.
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Shroomsh
Mad King



Registered: 07/28/15
Posts: 121
Loc: Washington
Last seen: 7 years, 3 months
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Re: Inoculating again after a week. [Re: Moabfighter]
#23478527 - 07/26/16 12:09 PM (7 years, 6 months ago) |
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I fail to see what is so scary about two strains in a jar. It's still a cubensis! What's really the worst that could happen? Other than contams obviously.
I think I might do it just to make you all mad
Edited by Shroomsh (07/26/16 12:09 PM)
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mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
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Re: Inoculating again after a week. [Re: Shroomsh]
#23478535 - 07/26/16 12:13 PM (7 years, 6 months ago) |
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Quote:
Shroomsh said: Oh yeah? And what will you have to say when I reinoc and it's all successful?
Don't be shy now, let's not always do the same thing now.
I tried that and got contams one the one jar I did it to on my first attempt, the dry verm barrier catches contams chances are even with a sterile needle you'll still pick up contams pushing through the already active verm barrier
Edit: thought you were doing Pf cakes
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
Edited by mrmazdarx9 (07/26/16 12:14 PM)
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Shroomsh
Mad King



Registered: 07/28/15
Posts: 121
Loc: Washington
Last seen: 7 years, 3 months
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Re: Inoculating again after a week. [Re: mrmazdarx9]
#23478542 - 07/26/16 12:15 PM (7 years, 6 months ago) |
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Quote:
mrmazdarx9 said:
Quote:
Shroomsh said: Oh yeah? And what will you have to say when I reinoc and it's all successful?
Don't be shy now, let's not always do the same thing now.
I tried that and got contams one the one jar I did it to on my first attempt, the dry verm barrier catches contams chances are even with a sterile needle you'll still pick up contams pushing through the already active verm barrier
Edit: thought you were doing Pf cakes
Mmm, this was really the biggest and only obstacle I foresaw as well. Think it's too risky to sterilize a spoon and scoop some verm outta the way?
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mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
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Re: Inoculating again after a week. [Re: Shroomsh]
#23478552 - 07/26/16 12:19 PM (7 years, 6 months ago) |
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Quote:
Shroomsh said:
Quote:
mrmazdarx9 said:
Quote:
Shroomsh said: Oh yeah? And what will you have to say when I reinoc and it's all successful?
Don't be shy now, let's not always do the same thing now.
I tried that and got contams one the one jar I did it to on my first attempt, the dry verm barrier catches contams chances are even with a sterile needle you'll still pick up contams pushing through the already active verm barrier
Edit: thought you were doing Pf cakes
Mmm, this was really the biggest and only obstacle I foresaw as well. Think it's too risky to sterilize a spoon and scoop some verm outta the way?
I'd wait it out after my experience but at the end of the day there your babies its down to you what you think is best
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
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h0ldthedoor
HODOR



Registered: 06/25/16
Posts: 510
Loc: North of The Wall
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Re: Inoculating again after a week. [Re: Shroomsh]
#23478565 - 07/26/16 12:26 PM (7 years, 6 months ago) |
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Quote:
Shroomsh said: Think it's too risky to sterilize a spoon and scoop some verm outta the way?
Wouldn't this involve opening the jar(s)? From what I've read it seems like a bad idea.
--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you. – Petyr Baelish
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Shroomsh
Mad King



Registered: 07/28/15
Posts: 121
Loc: Washington
Last seen: 7 years, 3 months
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Re: Inoculating again after a week. [Re: mrmazdarx9]
#23478578 - 07/26/16 12:32 PM (7 years, 6 months ago) |
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I'll wait a tad longer and if any show no signs of growth then we'll get jiggy with it.
Hodor, yes it would involve some opening, but what I would probably do is just puncture a small hole through both layers of foil and try to work with that and then add a 3rd layer to seal it again.
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: Inoculating again after a week. [Re: Shroomsh]
#23478851 - 07/26/16 01:53 PM (7 years, 6 months ago) |
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Quote:
Shroomsh said: Oh yeah? And what will you have to say when I reinoc and it's all successful?
I'd say: why are you wasting your time on cakes when grain spawn and bulk substrates will yield you more.
now I'm gonna go pour 80 petris and do real work. Good luck.
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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Shroomsh
Mad King



Registered: 07/28/15
Posts: 121
Loc: Washington
Last seen: 7 years, 3 months
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Re: Inoculating again after a week. [Re: filthyknees]
#23478927 - 07/26/16 02:17 PM (7 years, 6 months ago) |
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Quote:
filthyknees said:
Quote:
Shroomsh said: Oh yeah? And what will you have to say when I reinoc and it's all successful?
I'd say: why are you wasting your time on cakes when grain spawn and bulk substrates will yield you more.
now I'm gonna go pour 80 petris and do real work. Good luck.
Because this is only my 2nd grow and I'm just experimenting in various ways. I'm interested in using different teks but just haven't gotten to those parts yet. Once these cakes I have right now become colonized a partial amount of them will be transferred to a monotub that I'm gonna try out.
Have fun with your cultures! That's next on my list to learn.
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BaronVonBud
Stranger

Registered: 07/14/16
Posts: 143
Last seen: 7 years, 3 months
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Re: Inoculating again after a week. [Re: Shroomsh]
#23478970 - 07/26/16 02:30 PM (7 years, 6 months ago) |
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When you say that it was real clumpy does that mean you didn't shake it up?
-------------------- <iframe src="//giphy.com/embed/Z7TvUbQHuFdBu" width="480" height="352" frameBorder="0" class="giphy-embed" allowFullScreen></iframe><p><a href="http://giphy.com/gifs/rock-flag-eagle-Z7TvUbQHuFdBu">via GIPHY</a></p>
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