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Shop: Mushroom-Hut Liquid Cultures   Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore Injection Grain Bag   PhytoExtractum Maeng Da Thai Kratom Leaf Powder

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OfflineSirSippertonScrim
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Grain Jars
    #23472751 - 07/24/16 04:13 PM (7 years, 7 months ago)

Wondering if these yellow spots look like bacteria or mycelium metabolites to you guys? Was planning on using the jar for a g2g transfer but obviously with a bacterial contam this would be a lost cause. Could I salvage the jar in the event that it is bacteria by opening it and transferring some of the mycelium to agar in order to transfer away from the bacteria? Or should I just start over and order another syringe? Thanks in advance for any input.

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Offlinemrmazdarx9
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Re: Grain Jars [Re: SirSippertonScrim]
    #23472754 - 07/24/16 04:15 PM (7 years, 7 months ago)

Is it not just grains touching the glass that are a tad damp and havent got mycelium on them


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Edited by mrmazdarx9 (07/24/16 04:16 PM)

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Offlinespacechildo
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Re: Grain Jars [Re: mrmazdarx9]
    #23472772 - 07/24/16 04:21 PM (7 years, 7 months ago)

damn, how long has that been sitting? use colonized jars right away. i wouldn't g2g that.

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InvisibleKush_Zombie
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Re: Grain Jars [Re: spacechildo]
    #23472799 - 07/24/16 04:33 PM (7 years, 7 months ago)

Quote:

spacechildo said:
damn, how long has that been sitting? use colonized jars right away. i wouldn't g2g that.



Isn't it okay to let colonized jars sit if you rehydrate them?


--------------------
How to get started in bulk:
Presto 23-Quart Pressure Cooker
BOD's Simple as FUCK Still Air Box
PastyWhyte's Easy Agar Tek
Munchauzen's Cultivation Video Series
How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs)
What is G2G? (Grain-to-Grain)
Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary)
Spitball's Monotub Tek (A liner isn't necessary but is useful)
Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.

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Invisibleb1blazin
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Re: Grain Jars [Re: Kush_Zombie]
    #23472841 - 07/24/16 04:43 PM (7 years, 7 months ago)

ur good looks like metabolites to me ive let my jars sit and theyve done that

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OfflineSirSippertonScrim
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Re: Grain Jars [Re: b1blazin]
    #23472864 - 07/24/16 04:54 PM (7 years, 7 months ago)

They've been sitting for  a month exactly. Obviously not ideal but I had my hands full with some tubs and couldn't handle more volume at once. It's definitely not wet mycelium. If I shouldn't use it for g2g would it be possible to remove some mycelium and transfer it to agar or should I toss the whole thing? Thanks for the help!

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OfflineBobabouy
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Re: Grain Jars [Re: SirSippertonScrim]
    #23472871 - 07/24/16 04:56 PM (7 years, 7 months ago)

You can put some on Agar, no problem.

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Offlinespacechildo
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Re: Grain Jars [Re: SirSippertonScrim]
    #23472938 - 07/24/16 05:25 PM (7 years, 7 months ago)

Quote:

Kush_Zombie said:
Quote:

spacechildo said:
damn, how long has that been sitting? use colonized jars right away. i wouldn't g2g that.



Isn't it okay to let colonized jars sit if you rehydrate them?




depends how you see it, they start consolidating and lose vigor as they sit but they'll still produce.

Quote:

SirSippertonScrim said:
If I shouldn't use it for g2g would it be possible to remove some mycelium and transfer it to agar or should I toss the whole thing? Thanks for the help!




I would spawn it if its all you have find a 20qt mini mono!

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