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newtothis3
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Registered: 11/02/14
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Lids and contamination
#23471984 - 07/24/16 11:08 AM (7 years, 6 months ago) |
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Lately I've been getting more forest green contamination despite following PF tell, adding extra time to the sterilization at 15 psi, using a reputable vender, disinfecting my glove box, the bathroom I inoculate in and blocking the bathroom vent and door to stop air flow. I wear gloves and flame between jars. I wash the jars in my dishwasher and I've notice the lid sets have started to rust. I use medical tape over the holes after inculcation (and before). I'm thinking my kids and the seals must be degrading from dishwasher use. Do you guys think this is an explanation? Do you sterilize or boil your jars after birthing? Do you toss the jars that you've found contamination in? How do you get them ready to use again? I thought the dishwasher and sterilization in the pressure cooker was enough. Also how often do you replace your lids? I can't figure out what is going on and why I am getting more contamination than ever. Any advice is appreciated. Thanks!
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spacechildo
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Registered: 01/24/13
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Re: Lids and contamination [Re: newtothis3]
#23471991 - 07/24/16 11:12 AM (7 years, 6 months ago) |
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use a SAB instead of glovebox, dont inoc in the bathroom, that's where you shit! jars get sterilized again with new cake mix in them, just wash them normally first.
do you use a dry verm barrier and leave it dry and undisturbed? could also be a dirty syringe simply.
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TheChief
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Re: Lids and contamination [Re: newtothis3]
#23471994 - 07/24/16 11:13 AM (7 years, 6 months ago) |
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Stop using spore syringes. Start from agar and make your own LC if you wish to do cakes, spore syringes are always some what dirty at the very least.
Really you should think about getting into grains/bulk applications, transfering agar wedges to grain and then g2g from then on, it is much safer route than PF tek. Using grains and agar you can limit your contamination to almost zero.
Also, as spaceman suggests, make sure you are actually using a SAB and proper sterile technique. Not a glovebox.
Edited by TheChief (07/24/16 11:19 AM)
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spacechildo
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Re: Lids and contamination [Re: TheChief]
#23471998 - 07/24/16 11:15 AM (7 years, 6 months ago) |
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pf-tek is very forgiving tho, no recolonization of substrate needed so bacteria doesnt really matter or at least break a grow and no nasty endospores in brf.
But if you already got a PC I agree go to grains and agar. But I have a theory OP isnt following a proper pf-tek.
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weetsie
unlicensed tub surgeon



Registered: 05/08/11
Posts: 572
Loc: United Kingdom
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Re: Lids and contamination [Re: newtothis3]
#23472012 - 07/24/16 11:21 AM (7 years, 6 months ago) |
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>I'm thinking my kids and the seals must be degrading from dishwasher use. Do you guys think this is an explanation?
No, rusting lids are fine, the seals are not needed either, they are for people canning and need jars that will hold a vacuum, many people use them upside down, metal on glass without issue.
>Do you sterilize or boil your jars after birthing?
No, just clean them in the sink with soapy water and rinse them out.
> Do you toss the jars that you've found contamination in?
Some people do, I empty them outside then clean them. If they are full of mold spores you might wanna put your cloths straight in the wash afterwards, if they are just wet and full of bacteria then it's not so much of an issue.
> Also how often do you replace your lids?
I never do, even if they are rusted to hell, I won't replace them unless they get so bad they literally don't function as lids.
Your contamination could be lots of things, bathrooms are far from the cleanest room in the house for a start, spore syringes are never 100% even from vendors.
-------------------- Active grow logs: Oysters on Straw Pellets Trade list
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TheChief
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Quote:
spacechildo said: pf-tek is very forgiving tho, no recolonization of substrate needed so bacteria doesnt really matter or at least break a grow and no nasty endospores in brf.
But if you already got a PC I agree go to grains and agar. But I have a theory OP isnt following a proper pf-tek.
I also have a feeling the pf technique being used is the problem. OP needs a link to proper information.
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jcbowling1985 said: "Additionally I did one Armageddon jar with all of the types together."
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newtothis3
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Re: Lids and contamination [Re: TheChief]
#23472035 - 07/24/16 11:27 AM (7 years, 6 months ago) |
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I was following PF trek from the videos posted here. I do use a berm barrier. I was sterilizing in the bathroom after disinfecting with bleach spray and Lysol. I choose that room because it was the easiest to control air flow. I turn off every I got, blocked vent etc. I always wash clothes I've handed contaminated jars in the wash right away and open the bad jars outside. I never use to get such high contamination rates so I'm puzzled. What is a SAB? I haven't tried agar two at all, only PF tek thank you guys
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Bobabouy
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Re: Lids and contamination [Re: newtothis3]
#23472053 - 07/24/16 11:33 AM (7 years, 6 months ago) |
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Do not put tape over your holes. That's what the vermiculite is there for. (yes, I know I ended that sentence with a preposition).
Edited by Bobabouy (07/24/16 11:34 AM)
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TheChief
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Re: Lids and contamination [Re: newtothis3]
#23472067 - 07/24/16 11:37 AM (7 years, 6 months ago) |
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Quote:
newtothis3 said: What is a SAB?
This is your problem.
Use the search function and build a proper Still Air Box for doing clean work.
You are also trying to grow with very old information, it is no wonder you are getting a high contamination rate. You should search for methods posted by trusted cultivators in more recent years.
Edited by TheChief (07/24/16 11:40 AM)
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Bobabouy
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Re: Lids and contamination [Re: TheChief]
#23472075 - 07/24/16 11:40 AM (7 years, 6 months ago) |
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You shouldn't need a SAB for PF Tek. If your doing everything right, then it might be your syringe.
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spacechildo
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Re: Lids and contamination [Re: newtothis3]
#23472082 - 07/24/16 11:42 AM (7 years, 6 months ago) |
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Quote:
newtothis3 said: I was following PF trek from the videos posted here. I do use a berm barrier. I was sterilizing in the bathroom after disinfecting with bleach spray and Lysol. What is a SAB?
these videos www.mushroomvideos.com the "lets grow mushrooms" series? there's no lysol,bleach spray etc used there, and they explain the still air box.
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TheChief
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Re: Lids and contamination [Re: Bobabouy]
#23472087 - 07/24/16 11:42 AM (7 years, 6 months ago) |
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You don't need a toilet to take a shit either, but it makes things cleaner doesn't it?
I don't recommend doing any type of inoculation what so ever in open air.
Another thing, get your mushrooms out of the bathroom. Why are you innoculating where you shit and clean yourself? 
The only thing I feel confident in doing in the bathroom regarding growing mushrooms is dunking them, at that point they are 100% colonised and contams are not really a factor anymore.
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jcbowling1985 said: "Additionally I did one Armageddon jar with all of the types together."
Edited by TheChief (07/24/16 11:47 AM)
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Bobabouy
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Re: Lids and contamination [Re: TheChief]
#23472099 - 07/24/16 11:44 AM (7 years, 6 months ago) |
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What's a toilet? And a SAB will not fix a dirty syringe.
Edited by Bobabouy (07/24/16 11:46 AM)
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TheChief
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Re: Lids and contamination [Re: Bobabouy]
#23472113 - 07/24/16 11:48 AM (7 years, 6 months ago) |
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Quote:
Bobabouy said: What's a toilet? And a SAB will not fix a dirty syringe.
This is exactly why my first post in this thread is stop using spore syringes...
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jcbowling1985 said: "Additionally I did one Armageddon jar with all of the types together."
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Bobabouy
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Re: Lids and contamination [Re: TheChief]
#23472124 - 07/24/16 11:52 AM (7 years, 6 months ago) |
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Telling someone who uses their bathroom for inoculation to not use a syringe is kinda like pissing into the wind.
Edited by Bobabouy (07/24/16 11:54 AM)
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ToolTroll
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Re: Lids and contamination [Re: TheChief]
#23472129 - 07/24/16 11:54 AM (7 years, 6 months ago) |
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Is human feces really a contaminant in this case? I would think the constant moisture and the shower stall would be the main problem with a bathroom as clean room.
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Bobabouy
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Re: Lids and contamination [Re: ToolTroll]
#23472140 - 07/24/16 11:56 AM (7 years, 6 months ago) |
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You don't need a clean room for PF Tek. Wipe the lids with alcohol, flame the needle, inoculate.
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TheChief
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Re: Lids and contamination [Re: Bobabouy]
#23472141 - 07/24/16 11:56 AM (7 years, 6 months ago) |
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Quote:
Bobabouy said: Telling someone who uses their bathroom for inoculation to not use a syringe is kinda like pissing into the wind.
Well encouraging someone to do it seems kind of like you already pissed all over yourself many times in the past.
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jcbowling1985 said: "Additionally I did one Armageddon jar with all of the types together."
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Bobabouy
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Re: Lids and contamination [Re: TheChief]
#23472147 - 07/24/16 11:57 AM (7 years, 6 months ago) |
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Quote:
TheChief said:
Quote:
Bobabouy said: Telling someone who uses their bathroom for inoculation to not use a syringe is kinda like pissing into the wind.
Well encouraging someone to do it seems kind of like you already pissed all over yourself many times in the past.
You are super witty.
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TheChief
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Re: Lids and contamination [Re: Bobabouy]
#23472152 - 07/24/16 12:02 PM (7 years, 6 months ago) |
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I didn't grow pounds of dicks innoculating with spore syringes in a bathroom.
If you want to keep playing with contaminations for grams of shrooms, keep at your mentality.
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jcbowling1985 said: "Additionally I did one Armageddon jar with all of the types together."
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