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Invisibleoontribe
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Cobweb or they are germinating?
    #23471522 - 07/24/16 07:16 AM (7 years, 7 months ago)

So i inoculated 7 jars 3 days ago (usually they germinate after 3 days) and on the top of one of the jars i can see this:



A rhyzmo looking light white aerial myc on the top of the jar

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Invisiblepablokabute
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Re: Cobweb or they are germinating? [Re: oontribe]
    #23471564 - 07/24/16 07:40 AM (7 years, 7 months ago)

theres that yellowish spots, it can be sporulating mold. does it look that yellow in real life?


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InvisiblebodhisattaMDiscordReddit
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Re: Cobweb or they are germinating? [Re: pablokabute]
    #23471579 - 07/24/16 07:48 AM (7 years, 7 months ago)


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Invisibleoontribe
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Re: Cobweb or they are germinating? [Re: bodhisatta]
    #23471599 - 07/24/16 07:58 AM (7 years, 7 months ago)

No yellowing anywhere its the cam.

Thanks for the link mate...ill see if it grow 10× or something tomorrow.

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Invisibleoontribe
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Re: Cobweb or they are germinating? [Re: oontribe]
    #23472368 - 07/24/16 01:30 PM (7 years, 7 months ago)

Looking at it again...yes there is yellow color in there.

Should i toss?

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Invisiblepablokabute
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Re: Cobweb or they are germinating? [Re: oontribe]
    #23475100 - 07/25/16 10:32 AM (7 years, 7 months ago)

probably contaminated. toss it out and try again. you might wanna clean your spores up in agar culture first.

hows it lookin right now anyway?


--------------------

Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1

'The second seal: “All CONTAMINATED things and events are unsatisfactory.”'



"I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST."

--Anonymous Guerilla, or is he..

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Invisibleoontribe
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Re: Cobweb or they are germinating? [Re: pablokabute]
    #23475983 - 07/25/16 04:27 PM (7 years, 7 months ago)

I pcd it then tossed it...Unfortunately i cant work with agar so ill try again with a different spore print and make a new syringe...thanks for the help man.

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InvisibleMad Season
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Re: Cobweb or they are germinating? [Re: oontribe]
    #23476065 - 07/25/16 04:53 PM (7 years, 7 months ago)

I don't see anything that warranted a toss.. some discoloration but nothing saying mold. I'd have at least let it chill a day or 2 to see if it sporulates more.. Molds are like always granular. This looked like some slightly yellow myc imo.


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Invisibleoontribe
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Re: Cobweb or they are germinating? [Re: Mad Season]
    #23476123 - 07/25/16 05:06 PM (7 years, 7 months ago)

too late...lol, I have already tossed it.
and the other jars have a greenish color inside em that's why I thought this one too is contaminated.

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InvisiblePastywhyteMDiscord
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Re: Cobweb or they are germinating? [Re: oontribe]
    #23476285 - 07/25/16 05:58 PM (7 years, 7 months ago)

Looks like yeast. Not cobweb but certainly fucked.

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Invisibleoontribe
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Re: Cobweb or they are germinating? [Re: Pastywhyte]
    #23476450 - 07/25/16 06:35 PM (7 years, 7 months ago)

may i ask...so i can avoid this in the future.
is it a syringe problem or should i have pcd more or what?

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InvisiblePastywhyteMDiscord
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Re: Cobweb or they are germinating? [Re: oontribe]
    #23476467 - 07/25/16 06:40 PM (7 years, 7 months ago)

Could very well be the syringe. By pcd I'm going to assume you mean SFD. Provided the SFD are not worn out or damaged you only need one. It's doubtful a contam like yeast could penetrate a SFD and unlikely that all the SFD would fail in a single grow. Usually SFD fails are a one off occurrence.

If you want to track vectors you need to eliminate the ones out of your control. That means no more spore syringes.

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Offlinetommyg57

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Re: Cobweb or they are germinating? [Re: Pastywhyte]
    #23476568 - 07/25/16 07:04 PM (7 years, 7 months ago)

Pasty if I may ask what do you do to clean SFD? Is pcing not enough?

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InvisiblePastywhyteMDiscord
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Re: Cobweb or they are germinating? [Re: tommyg57]
    #23476584 - 07/25/16 07:07 PM (7 years, 7 months ago)

PCing is as clean as it gets. Clean is not the issue. Wear and tear is an issue but they can handle many many runs.

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Invisibleoontribe
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Re: Cobweb or they are germinating? [Re: Pastywhyte]
    #23476688 - 07/25/16 07:37 PM (7 years, 7 months ago)

By pc i meant pressure cooking, i use poly filters.
Thanks for the input mate tou are the best.

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InvisibleMad Season
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Re: Cobweb or they are germinating? [Re: oontribe]
    #23476827 - 07/25/16 08:14 PM (7 years, 7 months ago)

Quote:

Pastywhyte said:
Looks like yeast. Not cobweb but certainly fucked.



:fuckinawesome:

Yeah that first pic does look quite yeasty. I was thrown off thinking it was condensation on the myc. The last 2 did look like it was condensation lol. But yeah on 2nd look at the first pic I must agree.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.

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