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ichugwindex
Dex



Registered: 06/04/16
Posts: 4,613
Loc: ¯\_(ツ)_/¯
Last seen: 11 months, 2 days
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summertime blues
#23471247 - 07/24/16 02:25 AM (7 years, 6 months ago) |
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Started 18 half pint brf cakes and now im left with 7 (knocked up about 2 weeks ago still colonizing)i feel like the biggest failure now. I dont even know why im posting i guess i have nowhere else to vent. I used my SAB I steam sterilized everything and flamed all my syringes my surfaces where all bleach cleaned. I was so overwhelmed with sadness that i forgot to ask questions will micropore tape on my inoc/gas exchange holes help to decrease my contam rate? Is this really just the summertime blues?
-------------------- Only hope can give rise to the emotion we call despair. But it is nearly impossible for a man to try to live without hope, so I guess that leaves Man no choice but to walk around with despair as his companion.
Edited by ichugwindex (07/24/16 02:34 AM)
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Mp tape won't keep shit out. You also flame the needle red hot between jars right? If so sounds like you have dirty syringes. Agar cleanup will help greatly with that.
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ichugwindex
Dex



Registered: 06/04/16
Posts: 4,613
Loc: ¯\_(ツ)_/¯
Last seen: 11 months, 2 days
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Yes i torch the needles red hot. I guess i need to seriously get on learning agar. Probably didnt help these were all all MS. You know anything else I can do to lower my contam rate other than overviewing/revising my sterile tek?
Edited by ichugwindex (07/24/16 10:59 AM)
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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With pf tek, if you flame between jars and did in a sab, and have a decent dry verm layer, youre good on the sterile front. Sterile tek becomes big with agar and grains. Pf tek isn't like that. If you had lots contaminate, and you did a decent sterile tek, it most definitely is your inoculant that needs to be clean.
https://www.shroomery.org/forums/showflat.php/Number/19208976 awesome agar prep out of reusable containers
https://www.shroomery.org/forums/showflat.php/Number/18430998/fpart/1/vc/1 agar cleanup
https://www.shroomery.org/forums/showflat.php/Number/22833314#22833314 making clean syringes for pf tek out of agar
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ichugwindex
Dex



Registered: 06/04/16
Posts: 4,613
Loc: ¯\_(ツ)_/¯
Last seen: 11 months, 2 days
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Thank you so much for giving me some direction on this contam issue. I will definitely do my best to master the basics of agar and hopefully beyond! Im hoping to do this (temporarily) without a PC if at all possible.
-------------------- Only hope can give rise to the emotion we call despair. But it is nearly impossible for a man to try to live without hope, so I guess that leaves Man no choice but to walk around with despair as his companion.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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You can steam sterilize/boil in those containers.
https://www.shroomery.org/forums/showflat.php/Number/22901218
Lol he deleted the pics but they still expand anyways you can sterilize agar broth without a pc. Just gotta immerse in boiling water for like 60 minutes or something. Steam sterilization is basically the same shit, but not required at all.
Also forgot to ask what pf tek recipe/tek do you follow?
Edited by Mad Season (07/24/16 11:34 AM)
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ichugwindex
Dex



Registered: 06/04/16
Posts: 4,613
Loc: ¯\_(ツ)_/¯
Last seen: 11 months, 2 days
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You sir have saved my day.
-------------------- Only hope can give rise to the emotion we call despair. But it is nearly impossible for a man to try to live without hope, so I guess that leaves Man no choice but to walk around with despair as his companion.
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ichugwindex
Dex



Registered: 06/04/16
Posts: 4,613
Loc: ¯\_(ツ)_/¯
Last seen: 11 months, 2 days
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Did not see your question at the end. The tek i followed said 1 half cup verm 1 quarter cup brf and 1 quarter cup water per half pint container.
Steam sterilized my half pints for 90ish minutes and moved to my SAB then knocked up the next morning.
-------------------- Only hope can give rise to the emotion we call despair. But it is nearly impossible for a man to try to live without hope, so I guess that leaves Man no choice but to walk around with despair as his companion.
Edited by ichugwindex (07/24/16 12:21 PM)
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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And you mix the water with the verm first, then add the brf as a sort of "coating" for the verm right?
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ichugwindex
Dex



Registered: 06/04/16
Posts: 4,613
Loc: ¯\_(ツ)_/¯
Last seen: 11 months, 2 days
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Yessir water and verm in the mixing bowl then i add the the brf from my blender i use measuring cups to be precise on the measurements. Sorry for long reply time i have to post from my phone
-------------------- Only hope can give rise to the emotion we call despair. But it is nearly impossible for a man to try to live without hope, so I guess that leaves Man no choice but to walk around with despair as his companion.
Edited by ichugwindex (07/24/16 12:26 PM)
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