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TheChief
Cube Collector



Registered: 07/09/14
Posts: 1,905
Loc: Executing TEKs...
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Re: 8yr old spores [Re: Frommer]
#23472910 - 07/24/16 05:18 PM (7 years, 6 months ago) |
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Quote:
Frommer said: JDS.
Both are perfectly fine, been doing a lot of reading on this. Seen posts of prints like this 8+ years. Ive seen a post by RR with a slant of 7 years. Ive read of guys drying the mushroom and storing it for 10 years and bringing a sample back. Ive read about guys keeping LC in water for years.
The one thing Ive taken from all my reading is if you grow once a year and use the correct conditions you will always have something to fall back on.
The time frame for either cant be set in stone as it depends what conditions are met. That said If you take a print, dried mushroom and a slant and put them all in a cool dry place for 7 years I think you will find they all come back the same...
Im a noob... Hopefully some big dogs can add more sound info.
I know the cultures can live for a long ass time, even in dried mushroom tissue, like poster above suggests. Whether or not a culture or spore will be viable/strong enough to produce a mushroom to contine the life cycle (produce more spores), and how long they have before a said culture or spore cannot anymore is still up for debate. It will likely remain one until the end of time. This is a hard thing to measure without years and years of collective, factual research/documentation, and we are talking about cubensis mushrooms here; right? 
And then there is the which strains can last longer debate... 
The debate will never end.
I'm pretty sure one of the best way to store cultures is in sterile distilled water, and in the fridge around 1C, it halts all growth because there is no nutrients/keeps them hydrated. Spores or tissue may stay viable for a very long time this way, probably much longer than the 8 years suggested in this thread.
Edited by TheChief (07/24/16 05:38 PM)
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Frommer
Feeling jiggly the stripper up.


Registered: 02/08/16
Posts: 101
Loc: Amazon Jungle
Last seen: 6 years, 9 months
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Re: 8yr old spores [Re: TheChief]
#23472961 - 07/24/16 05:38 PM (7 years, 6 months ago) |
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Yes, 100% agreement.
Life cycle of culture dose not account for storage.
Any idea how long a cube culture can go? Say you always cloned from the last tub and kept going?
I must say, have seen a few posts pointing towards the distilled water. After whipping up 60 slants.
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TheChief
Cube Collector



Registered: 07/09/14
Posts: 1,905
Loc: Executing TEKs...
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Re: 8yr old spores [Re: Frommer]
#23473002 - 07/24/16 05:53 PM (7 years, 6 months ago) |
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Depends...
When are you taking the clones?
What is the maturity when taken?
Are you doing grain transfers?
Are you growing on agar and then grain each time or putting tissue right in a jar?
So many factors can go into the onset of senescence that make it hard to come up with an estimate like that.
If you took a fruit from each tub and made, lets say 5 grain jars out of one for another tub, from a very young clone tissue jar each time; it would probably take a few months before you start having issues (3 or 4 tubs I assume). These are just my estimates, this is just in regard to most cubes, other species of mushrooms may fare better.
Again, this is something I've never done and is all hear say.
It happens fairly quickly I've read, depending on what you are doing to the culture. Cloning of clones epeciallty causes cell degradation. This is why we try to keep our cultures as young as possible on agar and in slants/storage.
Edited by TheChief (07/24/16 06:07 PM)
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Frommer
Feeling jiggly the stripper up.


Registered: 02/08/16
Posts: 101
Loc: Amazon Jungle
Last seen: 6 years, 9 months
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Re: 8yr old spores [Re: TheChief]
#23473054 - 07/24/16 06:08 PM (7 years, 6 months ago) |
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Thanks, last Q.
Dose the age of the culture impact the spores? In my mind if you had a culture on last legs and took a print, what germinates is a fresh culture.
Is that correct?
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TheChief
Cube Collector



Registered: 07/09/14
Posts: 1,905
Loc: Executing TEKs...
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Re: 8yr old spores [Re: Frommer]
#23473077 - 07/24/16 06:13 PM (7 years, 6 months ago) |
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From my understanding you are correct, yes it would be a fresh, vigorous culture.
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jcbowling1985 said: "Additionally I did one Armageddon jar with all of the types together."
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Frommer
Feeling jiggly the stripper up.


Registered: 02/08/16
Posts: 101
Loc: Amazon Jungle
Last seen: 6 years, 9 months
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Re: 8yr old spores [Re: TheChief]
#23473104 - 07/24/16 06:22 PM (7 years, 6 months ago) |
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Thanks.
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BaronVonBud
Stranger

Registered: 07/14/16
Posts: 143
Last seen: 7 years, 3 months
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Re: 8yr old spores [Re: Frommer]
#23473179 - 07/24/16 06:39 PM (7 years, 6 months ago) |
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If you search the site you will find old post were the pros are telling people over a few years won't germinate
-------------------- <iframe src="//giphy.com/embed/Z7TvUbQHuFdBu" width="480" height="352" frameBorder="0" class="giphy-embed" allowFullScreen></iframe><p><a href="http://giphy.com/gifs/rock-flag-eagle-Z7TvUbQHuFdBu">via GIPHY</a></p>
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