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BaronVonBud
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Registered: 07/14/16
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8yr old spores
#23469525 - 07/23/16 02:42 PM (7 years, 6 months ago) |
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Put them under the scope and they are alive so far 2/4 have shown life under the scope
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YeOlde
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I have a spore syringe sent to me but am not in the position to grow just yet. I hear conflicting accounts of how to store them. Fridge or room temp? hmm.
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BaronVonBud
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Re: 8yr old spores [Re: YeOlde]
#23469653 - 07/23/16 03:17 PM (7 years, 6 months ago) |
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These were room temp for the entire 8 yrs and that's just in a house that fluctuates in temp A lot of ppl say the fridge is just a bacteria orgy
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Edited by BaronVonBud (07/23/16 03:19 PM)
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Josex
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So what's the question?
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BaronVonBud
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Re: 8yr old spores [Re: Josex]
#23469701 - 07/23/16 03:34 PM (7 years, 6 months ago) |
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Just statement, it that a rule?
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YeOlde
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Mmm yeah. I get the feeling fridge is a no no from what I've read.
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BaronVonBud
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Re: 8yr old spores [Re: YeOlde]
#23469873 - 07/23/16 04:27 PM (7 years, 6 months ago) |
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The fridge is often looked in by guest too
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Indigo-Child
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Most of my spores, both syringes and prints, are at least half of a decade old with the oldest going back about 12-13 years. Never had an issue with germination/contamination. For the record I keep them double bagged in the fridge.
Can I ask...How can you tell if spores are viable under a microscope? I actually work in microbiology and know no way of gauging viability via visual inspection, nor can I think of any, but am VERY interested in learning. I purchased about $200 worth of exotics (various pans, mexs, and woodlovers) from a VERY reputable exotic psilocybe dealer about five years back and was having SERIOUS issue with germination (none!). I was dying to try my hand at pans (particularly cambos) and only could get germination out of the Azures and that was only in one jar several years later. I've always maintained it was an issue with the syringes. I've posted about this here before, but nobody could give me a satisfactory answer and I do have a VERY nice microscope so it would be nice to see if the spores are in fact alive.
Thanks!
T.
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BaronVonBud
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Under the scope was kinda like SWIM
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MykoMykoAnay
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Are you going to put them on some agar???
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spacechildo
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Quote:
BaronVonBud said: Under the scope was kinda like SWIM
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Eywa_devotee
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IME spore syringes keep viability for several years in the fridge if double bagged to keep the nastiness out. I keep them in a mini fridge just for that purpose along with seeds, slants, and other stuff though.
Also you really can test spore viability under a microscope. All you need is to mix the spores with brown sugar water plop it on the slide and place the cover slip on. Wait several hours and look using cross polarized light. The viable spores will be seen with rainbow looking threads growing out of them. You can get suitable polarizers for this very cheaply from old calculators or watches with LCD displays. It's pretty trippy looking actually.
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TheChief
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Registered: 07/09/14
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Quote:
Eywa_devotee said: IME spore syringes keep viability for several years in the fridge if double bagged to keep the nastiness out. I keep them in a mini fridge just for that purpose along with seeds, slants, and other stuff though.
Also you really can test spore viability under a microscope. All you need is to mix the spores with brown sugar water plop it on the slide and place the cover slip on. Wait several hours and look using cross polarized light. The viable spores will be seen with rainbow looking threads growing out of them. You can get suitable polarizers for this very cheaply from old calculators or watches with LCD displays. It's pretty trippy looking actually.

I thought something like that as well when reading this thread before, if you just put some nutrient on the slide, then you would be able to see them germinating; right? I just wasn't sure so I didn't bother posting about it.
This is awesome information, thank you for posting!
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jcbowling1985 said: "Additionally I did one Armageddon jar with all of the types together."
Edited by TheChief (07/24/16 03:53 PM)
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mrmazdarx9
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Quote:
BaronVonBud said: Under the scope was kinda like SWIM
Ah that clears that up then. Indigo child will be happy with that detailed answer
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Frommer
Feeling jiggly the stripper up.


Registered: 02/08/16
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Thanks for the post, been doing a lot of reading with regards to how long storage methods last. Its a nice thing for a noob stocking up prints to hear about them keeping.
My question to you is, what is the purpose of viewing germination at this point? Are you able to identify something more than what agar would show?
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mupetmower
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Registered: 03/29/16
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Quote:
Eywa_devotee said: The viable spores will be seen with rainbow looking threads growing out of them. You can get suitable polarizers for this very cheaply from old calculators or watches with LCD displays. It's pretty trippy looking actually.
that sounds like it would be really, really cool to see!
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TheChief
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Re: 8yr old spores [Re: Frommer]
#23472855 - 07/24/16 04:49 PM (7 years, 6 months ago) |
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Quote:
Frommer said: Thanks for the post, been doing a lot of reading with regards to how long storage methods last. Its a nice thing for a noob stocking up prints to hear about them keeping.
My question to you is, what is the purpose of viewing germination at this point? Are you able to identify something more than what agar would show?
I can imagine it being useful in not having to wait or waiste materials/time on trying to get them to germinate plates. You would be able to see a lot clearer and faster what is really going on under a scope, before deciding to invest the materials and time.
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jcbowling1985 said: "Additionally I did one Armageddon jar with all of the types together."
Edited by TheChief (07/24/16 04:52 PM)
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jds


Registered: 10/06/08
Posts: 3,083
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Re: 8yr old spores [Re: TheChief]
#23472860 - 07/24/16 04:51 PM (7 years, 6 months ago) |
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What should last longer between a spore print and a spore syringe? I have some spore prints hidden away in dark, dry, cool corners in case anything were to happen and I had to restart everything from scratch, but I'm not sure how long they'll keep or how often I should take more prints to replace them.
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Frommer
Feeling jiggly the stripper up.


Registered: 02/08/16
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Re: 8yr old spores [Re: TheChief]
#23472870 - 07/24/16 04:56 PM (7 years, 6 months ago) |
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I was thinking about that, but curious to know if we have any plus sides to growth patterns. If it can serve more purpose in working with Myc, Ill be doing the trek home to grab my kit. Havnt done much scope work in years, would be cool if it had more application here.
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Frommer
Feeling jiggly the stripper up.


Registered: 02/08/16
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Re: 8yr old spores [Re: jds]
#23472882 - 07/24/16 05:03 PM (7 years, 6 months ago) |
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JDS.
Both are perfectly fine, been doing a lot of reading on this. Seen posts of prints like this 8+ years. Ive seen a post by RR with a slant of 7 years. Ive read of guys drying the mushroom and storing it for 10 years and bringing a sample back. Ive read about guys keeping LC in water for years.
The one thing Ive taken from all my reading is if you grow once a year and use the correct conditions you will always have something to fall back on.
The time frame for either cant be set in stone as it depends what conditions are met. That said If you take a print, dried mushroom and a slant and put them all in a cool dry place for 7 years I think you will find they all come back the same...
Im a noob... Hopefully some big dogs can add more sound info.
Edited by Frommer (07/24/16 05:10 PM)
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