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Offlinecandela
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Registered: 09/04/15
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Last seen: 2 years, 7 months
Slants vs. Spore Prints
    #23469023 - 07/23/16 11:22 AM (7 years, 7 months ago)

For very, very, very long term storage, years, which is the more reliable method.

Edited by candela (07/23/16 11:23 AM)

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OfflineYaMoonSun
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Registered: 10/23/14
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Re: Slants vs. Spore Prints [Re: candela]
    #23469050 - 07/23/16 11:32 AM (7 years, 7 months ago)

I've used 7 year old hand-me-down syringes that were donated without a problem. I think it's because they were hydrated though, and possibly even refrigerated prior to me getting them. Slants are good if you want to store an isolate for a long time.

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InvisiblePastywhyteMDiscord
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Registered: 09/15/12
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Re: Slants vs. Spore Prints [Re: YaMoonSun]
    #23469071 - 07/23/16 11:43 AM (7 years, 7 months ago)

Slants are only good for 2-4 years. But the culture in them can be keep alive for decades. Much longer than a print would last. Just need to rotate the culture.

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OfflineEywa_devotee
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Re: Slants vs. Spore Prints [Re: Pastywhyte]
    #23469343 - 07/23/16 01:34 PM (7 years, 7 months ago)

Like apples and oranges, agar slants are for saving the purified strain, and prints are for species archival.

As above make sure to start new slants from the culture every two years or so. Spore prints will keep indefinitely if allowed to dry thoroughly and are stored at near freezing temperatures. You can make a spore liquid out of water, glycerin, and polyethylene glycol that will allow you to keep spores at freezer temperatures indefinitely as well.

FWIW, I tested some prints that were 7 years old that were kept in less than ideal conditions- kept in an unheated and uncooled storage locker for 3 of the years- and they still were very much viable.


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