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overthrow
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Registered: 07/22/16
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Last seen: 7 years, 2 months
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Low on spores, safest way?
#23466711 - 07/22/16 04:54 PM (7 years, 6 months ago) |
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This is my first time cultivating shrooms and I started the whole process by sending a letter to Free Spore Ring Earth. It took a month before I received the B+ spore print back.
In the meantime, I spent a lot of time reading through this forum to gather information and be prepared for when I actually had the print. I collected every item required for the PF Tek with the exception of the 1/2 pint jars which simply do not exist where I live (I've looked everywhere online and on multiple stores). So instead I bought regular glasses and I'm using aluminum foil + rubber bands to seal.
I have also built a glovebox which seems to have turned out really well.
And, I have the required items to make this liquid culture. I'd be using honey instead of Karo though since I've read it works.
Now...
The spore print that I got back is really small. I have not yet opened it so I don't know if it is any good. I've already sent them another letter and am going to buy prints online now that I know I can actually receive them here without a problem. However, it will take at least a month before I have a chance to put my hands on another print.
I am as of right now steaming (90min on regular pan) the BRF glasses + a shot glass also topped with aluminum foil that I will remove once inside the GB. The recipe of 2 cups vermiculte, 1 cup BRF and 1 cup water gave me exactly two glasses instead of 4-5 jars as per the tut.
Will that be a problem?
And, considering I have limited spores, the options that I can think of are: #1 Make the liquid culture first so I have more room to screw up later (considering I don't fuck this up) #2 Inoculate these two glasses (and if I have any left then I do #1).
I guess my main question is what are the chances of success of #1 or #2 in my specific case? Other ideas?
Edited by overthrow (07/22/16 08:42 PM)
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mrmazdarx9
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Registered: 05/15/16
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Re: Low on spores, safest way? [Re: overthrow]
#23466757 - 07/22/16 05:09 PM (7 years, 6 months ago) |
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How big are those glasses there is a reason they fill 4-5 jars because large jars tend to stall with that amount of Pf mix I can usually do 4-5 half pint jars with a bit to spare Where do you live I struggled with jars in UK but jam jars pretty much the same as the american ball/Kerr jars plus I just found ball jars cheap enough for me to buy 40 quid for 18 nearly 2 quid a jar ain't too bad
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Edited by mrmazdarx9 (07/22/16 05:36 PM)
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lukehighwalker710
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Registered: 03/04/16
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Re: Low on spores, safest way? [Re: mrmazdarx9] 1
#23466777 - 07/22/16 05:18 PM (7 years, 6 months ago) |
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I don't know anything about the PF tek so I can't help ya there..
But a glovebox is outdated equipment, nowadays cultivators use an SAB (still air box) which is similar but without the gloves attached because they cause more problems than good.
The print is plenty big, no matter how big it really is. First, don't open it until in a proper SAB and ready to be used. But there are enough spores on one print to make many syringes! You just need a tiny scraping off the orint to work with.
Spores to LC is (almost) guaranteed to have problems. The proper way to start an LC is with agar which is how I suggest you go about germinating your spores in the first place. Then transfer till you have clean growth. Then you can start and LC or Li if you wanted.
Invest in a pressure cooker!!! Seriously.
Edit: if you're trying to make cakes in a drinking glass with a tinfoil lid you're destined for failure. You need to do a lot more reading, and get the proper equipment ready.
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Highwalker
Edited by lukehighwalker710 (07/22/16 05:20 PM)
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spacechildo
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Registered: 01/24/13
Posts: 19,243
Loc: Babylon
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follow www.mushroomvideos.com vid 1-4 and do it right, that's the easiest way to guarantee at least 1 mushroom to print and do more grows with!
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overthrow
Creator



Registered: 07/22/16
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Re: Low on spores, safest way? [Re: spacechildo]
#23467119 - 07/22/16 07:04 PM (7 years, 6 months ago) |
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The glasses are about 400ml.
I have spent a full month looking for the 1/2 pint wide mouth jars and they definitely do not exist where I live. I have looked online, in specialized stores as well as in the grocery store for products that come in jars that I could possibly use and absolutely none of them would work for not being shoulderless.
I will have to work with whatever the second best solution is, even if I have to buy smaller glasses or whatever. I'll be in the US in December and will bring jars from there.
Also I have read that the ideal definitely is to have a PC and I will surely invest in a good one eventually but for now I'll have to make do with a regular pan. I've read that for the PF Tek it is completely viable to do a 90min roll and I guess I could watch my BRF glasses without inoculating just to see if nasty things grow out of them.
As for the glove box, I had no idea, I'll look into it. I can easily detach the gloves and make adjustments.
Considering that my glasses might be too big and the growth might stop, I am pending on going with this other LC tek that I just found.
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spacechildo
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Registered: 01/24/13
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Re: Low on spores, safest way? [Re: overthrow]
#23467182 - 07/22/16 07:19 PM (7 years, 6 months ago) |
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jars should be around 250mL / 1 cup and be as tall as they are wide. everything you need to know about SABs is in the videos.
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lukehighwalker710
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Re: Low on spores, safest way? [Re: spacechildo]
#23467239 - 07/22/16 07:35 PM (7 years, 6 months ago) |
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Why not just scrape some of the spores into a jar of sterilized water and suck up the spore laden water in a syringe and use that to nocc your cakes? I feel like that'd be safer than going to LC, where the nutrients and sugars can allow bacteria and contams to flourish. Also, you can get plastic (pp5) jars and lids and use those, however I don't know about boiling them just PCing. But whatever you use, it needs to have a proper fitting lid.
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Highwalker
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overthrow
Creator



Registered: 07/22/16
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Last seen: 7 years, 2 months
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The answers here have been really helpful so far but I'm still kinda lost. In the LC tek that I just linked to he mentions his first grow ever starting with a LC.
My cakes are probably too big and of course not the best solution which would have been the jars. I'm still pissed about not being able to find them here. However, I have seen a video (check below @ 6m40s) where the dude is using 3 sheets of aluminum foil on a jar so I thought a glass should do the trick too.
That's why I am still pending on trying the LC so I can fuck up later. Earlier replies made me rather pessimistic about trying the cakes. In case I don't have enough to inoculate them + make the LC at least I'll leave one to see if I would have had contamination.
Also for a noob it's kind of tricky to know what is outdated and what is the norm. I totally had no clue about the glovebox. Now I am really curious why no gloves attached is better and I will do the research before I prepare to make the syringe tomorrow.
By the way I watched the videos on www.mushroomvideos.com multiple times. It just sucks that I can't follow them 100% for living in a place where I can't find shit. It would have been a no brainer if I had the right containers.
Edited by overthrow (07/22/16 08:38 PM)
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: Low on spores, safest way? [Re: overthrow]
#23467686 - 07/22/16 10:04 PM (7 years, 6 months ago) |
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You should really look into agar you can stretch those spores out to months and months worth of grows ... and Goto Amazon.com for the proper jars idk where you live but Amazon has all you need and if you get a PC get either the 16 or 23 quart they are from 60-96$ on there
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Inocuole
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Registered: 11/21/11
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Re: Low on spores, safest way? [Re: Boogieman47]
#23468159 - 07/23/16 01:18 AM (7 years, 6 months ago) |
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Do not try to use LC to stretch out spores, that is not the purpose. Use agar to stretch out spores, and use that to incoulate LC.
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oontribe


Registered: 01/14/15
Posts: 3,570
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Re: Low on spores, safest way? [Re: Inocuole]
#23468281 - 07/23/16 03:09 AM (7 years, 6 months ago) |
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Use SAB.
Make spore syringe as its the easiest way for noobs not lc but if you can use agar then go for it.
Did you find perlite?
Good luck mate
Edited by oontribe (07/23/16 03:10 AM)
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overthrow
Creator



Registered: 07/22/16
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Last seen: 7 years, 2 months
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Re: Low on spores, safest way? [Re: oontribe]
#23468329 - 07/23/16 05:21 AM (7 years, 6 months ago) |
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Yes, I have found perlite here but no use until I have the cakes ready.
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overthrow
Creator



Registered: 07/22/16
Posts: 10
Last seen: 7 years, 2 months
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Re: Low on spores, safest way? [Re: overthrow]
#23468335 - 07/23/16 05:32 AM (7 years, 6 months ago) |
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Guys thanks for all the answers. So I've learnt the best/safest way is agar using a SAB.
And yes I know how important a PC is. However I've read in different places + seen in the RogerRabbit videos him using a regular pot to steam for 90min. Now, I know I am a noob but the dude knows what he is doing and apparently for the PF Tek it is not absolutely necessary. In his videos he does use a SAB and it was my mistake to think that he could do that for being a pro. Still on my list to get a PC though.
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mrmazdarx9
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Registered: 05/15/16
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Re: Low on spores, safest way? [Re: overthrow]
#23468345 - 07/23/16 05:40 AM (7 years, 6 months ago) |
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Quote:
overthrow said: Guys thanks for all the answers. So I've learnt the best/safest way is agar using a SAB.
And yes I know how important a PC is. However I've read in different places + seen in the RogerRabbit videos him using a regular pot to steam for 90min. Now, I know I am a noob but the dude knows what he is doing and apparently for the PF Tek it is not absolutely necessary. In his videos he does use a SAB and it was my mistake to think that he could do that for being a pro. Still on my list to get a PC though.
That's the point though mate he does know what he's doing its better for a newbie to PC it'll give you a much better chance of success
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
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Yerow
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Re: Low on spores, safest way? [Re: mrmazdarx9]
#23468356 - 07/23/16 05:50 AM (7 years, 6 months ago) |
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You can make agar without a pressure cooker too.
If i were you i would find some agar agar first of all, find a recipe that suits you. Easiest would be something like Yeast+agaragar+malt extract or PDYA.
those ingredients are super easy to find in any town, you'll have to get petri dishes or do it the pasty way: https://www.shroomery.org/forums/showflat.php/Number/19208976
So..
spore print -> LC -> 2 options Failure/Win the way i see it  Spore print -> agar -> infinite options such as clean LC, LI and whatever
Should also say if you go the agar way, then you will be able to use that B+ print for 1000's of jars
Edited by Yerow (07/23/16 05:52 AM)
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mrmazdarx9
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Registered: 05/15/16
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Re: Low on spores, safest way? [Re: Yerow]
#23468358 - 07/23/16 05:52 AM (7 years, 6 months ago) |
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Or just buy some premade PDA its not expensive really to have a play with before making your own in large quantities buying all the ingredients and equipment
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
Edited by mrmazdarx9 (07/23/16 05:53 AM)
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spacechildo
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Registered: 01/24/13
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Re: Low on spores, safest way? [Re: overthrow]
#23468476 - 07/23/16 07:30 AM (7 years, 6 months ago) |
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Quote:
overthrow said: Guys thanks for all the answers. So I've learnt the best/safest way is agar using a SAB.
And yes I know how important a PC is. However I've read in different places + seen in the RogerRabbit videos him using a regular pot to steam for 90min. Now, I know I am a noob but the dude knows what he is doing and apparently for the PF Tek it is not absolutely necessary. In his videos he does use a SAB and it was my mistake to think that he could do that for being a pro. Still on my list to get a PC though.
you dont need a PC for pf-tek but you really dont need agar for it either.
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Boogieman47
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Registered: 03/05/16
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Re: Low on spores, safest way? [Re: spacechildo]
#23469415 - 07/23/16 02:00 PM (7 years, 6 months ago) |
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That's not why we are telling him to get all the stuff he was trying to put his only spore print straight into LC
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overthrow
Creator



Registered: 07/22/16
Posts: 10
Last seen: 7 years, 2 months
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Re: Low on spores, safest way? [Re: Boogieman47]
#23469506 - 07/23/16 02:35 PM (7 years, 6 months ago) |
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Today I made two 10cc syringes using a bit less than half of the print. I had no idea whether it would be packed or what. It was a very decent print of a very small shroom.
So in the end I might be able to try all of the above and if I fail everything including the syringes I can still make, then I guess I need the month's wait to do more research and equipment improvement.
Learning about agar now and also I might have to move away from cakes and maybe think of grains or something else since I definitely can find jars, just not the ones appropriate for the PF Tek.
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Boogieman47
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Registered: 03/05/16
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Re: Low on spores, safest way? [Re: overthrow]
#23469520 - 07/23/16 02:41 PM (7 years, 6 months ago) |
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Goto Amazon.com can't you order from there where you live ?
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