|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
bloodwater
Stranger
Registered: 06/28/16
Posts: 21
Last seen: 7 years, 6 months
|
To clone or to islotate.
#23465645 - 07/22/16 11:09 AM (7 years, 6 months ago) |
|
|
Have multispore dishes. Some where used to go to grain to make my first tub.
I still have more dishes.
Now the question is do I want to Islote from dishes or to clone from fruit.
Trying to get an answer on this. Both seem good
Isolation from spores +You get one isolate +young genetics +same results every time -might not fruit -could be bad yielding, low potency, looks funny
Cloning from bulk +can get better fruits due to more than 1 isolate -older genetics so more prone to mutations and failure -results can vary
|
CosmoKramer
The Assman

Registered: 06/22/16
Posts: 555
|
Re: To clone or to islotate. [Re: bloodwater]
#23465812 - 07/22/16 12:01 PM (7 years, 6 months ago) |
|
|
Can't go wrong with doing both if you have extra dishes to spare.
-------------------- "Get yourself some vitamin C with rose hips and bioflavonoids."
|
bloodwater
Stranger
Registered: 06/28/16
Posts: 21
Last seen: 7 years, 6 months
|
Re: To clone or to islotate. [Re: CosmoKramer]
#23465839 - 07/22/16 12:11 PM (7 years, 6 months ago) |
|
|
True that, true that
Just don't want to waste my time.
|
spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
|
Re: To clone or to islotate. [Re: CosmoKramer]
#23465845 - 07/22/16 12:14 PM (7 years, 6 months ago) |
|
|
Isolation takes a LONG time and lots of transfers, just clone a pin that starts growing on your agar plates after 3-4 weeks. clones arent more prone to mutations or failure in any way, so just forget about that.
|
bloodwater
Stranger
Registered: 06/28/16
Posts: 21
Last seen: 7 years, 6 months
|
Re: To clone or to islotate. [Re: spacechildo]
#23465855 - 07/22/16 12:17 PM (7 years, 6 months ago) |
|
|
It is older gentics, so wouldn't it be more prone?
|
Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
|
Re: To clone or to islotate. [Re: bloodwater]
#23465859 - 07/22/16 12:18 PM (7 years, 6 months ago) |
|
|
Quote:
bloodwater said: to make my first tub.
Because of this, I will say stick to clones.
Also, you don't seem to know enough about these to fully commit to an isolation project. An isolation project can take months, you can easily burn through 500 petri dishes doing it. An isolate has the ability to be better than a clone because it is only 1 strain. Such isolates are hard to get, there's a lot of trial, error and repeat. When isolating, you need to end up with a bunch of isolates to get something worthwhile, still probably not enough to get something that will beat a very good clone.
Clones can be stabilized a bit by doing isolation to get rid of the multiple strains that weren't compatabile for anastomosis. When you do this, results will still vary but to a lesser, more predictable degree. It's true, a clone will not last as long as an isolate but it will still last years if taken care of properly. If you make multiple slants right after taking a clone, you're set for a pretty long time.
When you do decide to go for isolation, keep it as a side project. Keep growing whatever it is that interests you in the meantime. As I said, it takes a while to go from spores to an isolate, it even takes a considerable amount of time and effort to test every isolate you get and compare to see which is best. In the end, you have no guarantee that you isolate will be a killer, you might easily end up having better clones, causing you to start from scratch.
|
lukehighwalker710
Cloud 9 Dweller



Registered: 03/04/16
Posts: 609
Loc: out there
Last seen: 6 years, 22 days
|
Re: To clone or to islotate. [Re: CosmoKramer]
#23465862 - 07/22/16 12:19 PM (7 years, 6 months ago) |
|
|
To get a true isolate requires a lot of work. You'll go through 100s of plates to get one you want to keep because you have to transfer till you get sectoring then transfer each sector to a new plate and continue to do so till you have no more sectoring and then each isolate must be tested and hopefully one of the many sectors you'll have will have all the characteristics you want.
Not saying not to undertake the journey, just want you to know what you getting into. A clone is a much faster way to get decent results with some variability as the genetic pool is much smaller, but still a pool. Like Cosmo said, I would (and am) do(ing) both. Work on isolation as a side project, as it will basically just be doing transfers every so often and in the meantime.. You have your first grow going so select some good looking clone candidates and get those on plates to work with to produce with.
Edit:damn, supa beat me to it.
--------------------
Highwalker
Edited by lukehighwalker710 (07/22/16 12:19 PM)
|
spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
|
Re: To clone or to islotate. [Re: bloodwater]
#23465872 - 07/22/16 12:21 PM (7 years, 6 months ago) |
|
|
Quote:
bloodwater said: It is older gentics, so wouldn't it be more prone?
no its still very young genetics because you clone pins and not mature mushrooms. a pin from a plate hasnt done much cell divisions at all.
|
bloodwater
Stranger
Registered: 06/28/16
Posts: 21
Last seen: 7 years, 6 months
|
|
Well better rephrase that. This is my first tub in a few years.
I have gotten my own isolates down in the past but never fruited them. By than I scrapped the projects and went on with life.
I think I will need up attempting isolates a long side clones.
|
bloodwater
Stranger
Registered: 06/28/16
Posts: 21
Last seen: 7 years, 6 months
|
Re: To clone or to islotate. [Re: spacechildo]
#23465896 - 07/22/16 12:27 PM (7 years, 6 months ago) |
|
|
[quote=spacechildo]no its still very young genetics because you clone pins and not mature mushrooms. a pin from a plate hasnt done much cell divisions at all.
well wouldn't I want to let the mushroom grow out to see the end result and clone from the base of the mushroom inside?
Edited by bloodwater (07/22/16 12:31 PM)
|
Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
|
Re: To clone or to islotate. [Re: spacechildo]
#23465900 - 07/22/16 12:28 PM (7 years, 6 months ago) |
|
|
Even cloning mature shrooms won't cause mutations. What you call mutations is actually the aging of the culture, commonly known as senescene. Once you take a clone, you slant it, optionally you isolate it and then you test it. If it is crap, empty the slant but if it's good, treat that slant like gold.
A slant will barely age at all and it takes a lot of wedges to totally use it up. Every wedge can inoculate a plate, that plate can inoculate hundreds of plates, each plate can inoculate multiple jars. You get my drift. When your slant is almost finished, take another wedge, put it on agar, see if it's clean and slant it again. If you do this, the 2nd slant will still be a ways away from senescening and not too much older than your first clone transfer.
|
lukehighwalker710
Cloud 9 Dweller



Registered: 03/04/16
Posts: 609
Loc: out there
Last seen: 6 years, 22 days
|
Re: To clone or to islotate. [Re: bloodwater]
#23465905 - 07/22/16 12:30 PM (7 years, 6 months ago) |
|
|
Quote:
bloodwater said:
well wouldn't I want to let the mushroom grow out to see the end result and clone from the base of the mushroom inside?
He said to clone a pin that grows in vitro on agar.
--------------------
Highwalker
|
Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
|
Re: To clone or to islotate. [Re: bloodwater]
#23465908 - 07/22/16 12:31 PM (7 years, 6 months ago) |
|
|
Quote:
bloodwater said: [quote/]no its still very young genetics because you clone pins and not mature mushrooms. a pin from a plate hasnt done much cell divisions at all.
well wouldn't I want to let the mushroom grow out to see the end result and clone from the base of the mushroom inside?
I prefer this, invitro clones are a little less predictable. That said, people still get killer clones from invitro pins and it's not uncommon to get a culture that is better than your average MS grow.
Invitro clones have their own desirable traits. They are fast to get, young and they have already shown you that they can pin. Cloning from mature fruits will give you a higher probability of getting a tub full of your favourited mushroom that you originally cloned.
|
Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 1 day, 8 hours
|
|
I recommend doing an an MS grow from afar inoculation, taking a clone from a mature fruit, then if you want you can isolate a single strain out of that clone that is guaranteed to have fruiting genetics....that's how I did my isolate.
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
|
spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
|
|
Quote:
lukehighwalker710 said:
Quote:
bloodwater said:
well wouldn't I want to let the mushroom grow out to see the end result and clone from the base of the mushroom inside?
He said to clone a pin that grows in vitro on agar.
yeah you clone pins so you cant see the final outcome but clusters, fat first pins etc are good candidates but so are usually invitro clones on agar. so you can do both ways, yeah, I just recommended 1 over the other.
You still have to test your clone to see how it performs, get to know what it likes and how it behaves in different situations.
|
Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
|
|
It's not a true isolate though, so it's not what OP is referring to. Isolating a clone will make it more stable however so it's worth doing. I would still refer to an isolated clone as a clone though.
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
|
|
Don't forget that invitro pins often mean they can grow with shit FAE and little water or nutes. Once you get them on a nutrition and water rich sub in decent FAE they often really go nuts. My best clones are all from invitro pins and I have yet to see anyone throw up an isolate that can compete with my high producers pound for pound.
|
bloodwater
Stranger
Registered: 06/28/16
Posts: 21
Last seen: 7 years, 6 months
|
|
"invitro pins"
trying to find what this is now. Search function just has people talking about them.
Are they just your first few pins?
|
Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 1 day, 8 hours
|
Re: To clone or to islotate. [Re: Pastywhyte]
#23465927 - 07/22/16 12:38 PM (7 years, 6 months ago) |
|
|
Quote:
Pastywhyte said: Don't forget that invitro pins often mean they can grow with shit FAE and little water or nutes. Once you get them on a nutrition and water rich sub in decent FAE they often really go nuts. My best clones are all from invitro pins and I have yet to see anyone throw up an isolate that can compete with my high producers pound for pound.
We'll see about that in a few weeks
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
|
spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
|
Re: To clone or to islotate. [Re: bloodwater]
#23465943 - 07/22/16 12:42 PM (7 years, 6 months ago) |
|
|
Quote:
bloodwater said: "invitro pins"
trying to find what this is now. Search function just has people talking about them.
Are they just your first few pins?
its pins that form before you fruited your sub, invitro means "in the glass", but here we refer to pins that start on your agar plate a few weeks after full colonization.
|
mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
|
Re: To clone or to islotate. [Re: Pastywhyte] 1
#23465944 - 07/22/16 12:42 PM (7 years, 6 months ago) |
|
|
Quote:
Pastywhyte said: Don't forget that invitro pins often mean they can grow with shit FAE and little water or nutes. Once you get them on a nutrition and water rich sub in decent FAE they often really go nuts. My best clones are all from invitro pins and I have yet to see anyone throw up an isolate that can compete with my high producers pound for pound.
this is kinda what i was thinking(and hoping) might be the case for the clones i have taken from plates.
and OP, in this context, the in-vitro pin refers to a pin that is pinning on an agar plate. that way you dont have to actually fruit anything to see if it will fruit.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
|
Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
|
Re: To clone or to islotate. [Re: Pastywhyte]
#23465960 - 07/22/16 12:47 PM (7 years, 6 months ago) |
|
|
Quote:
Pastywhyte said: Don't forget that invitro pins often mean they can grow with shit FAE and little water or nutes. Once you get them on a nutrition and water rich sub in decent FAE they often really go nuts. My best clones are all from invitro pins and I have yet to see anyone throw up an isolate that can compete with my high producers pound for pound.
Or they can turn out to be mad pinners but buzzkill growers like the ones we were talking about in the other thread 
When it comes to cloning, I think one should do both. Cloning a mature fruit will have a better chance of turning into what you wanted it to look like but invitro clones are easy to get, just neglect a plate and clone it once it grows. I like to leave a plate of each new variety I get solely for invitro pins.
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
|
|
I usually look for invitro pins that pop up right away. Pins that show after the plate sits around for 3 months are usually not worth my time.
|
mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
|
Re: To clone or to islotate. [Re: Pastywhyte]
#23465974 - 07/22/16 12:53 PM (7 years, 6 months ago) |
|
|
thats what i did. after i transfer i let my palte sit for a couple weeks. if it clones soon after it finished colonizing the plate, then ill take it, and if not after a week or two after it colonizes the whole plate, i toss it.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
|
Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
|
Re: To clone or to islotate. [Re: Pastywhyte]
#23466116 - 07/22/16 01:51 PM (7 years, 6 months ago) |
|
|
Quote:
Pastywhyte said: I usually look for invitro pins that pop up right away. Pins that show after the plate sits around for 3 months are usually not worth my time.
Well that is disheartening, my one and only PE invitro pin took months to form. The only one that pinned within weeks for me was martinique, that one is quite fierce. I'm still waiting for my AA+, F+ and GT to pin but I think they have been there for at least 3-4weeks.
Would you say the amount of nutrients has an effect on how fast invitro pins form? I use a nutrient poor MEA recipe almost exclusively.
|
Bobabouy
Shrimp Boat Captain



Registered: 01/19/16
Posts: 661
Loc: The Black Sea
Last seen: 6 years, 16 days
|
|
But c10h12n2o said I could have an isolate in just a few transfers.
|
Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 1 day, 8 hours
|
Re: To clone or to islotate. [Re: Bobabouy]
#23466171 - 07/22/16 02:08 PM (7 years, 6 months ago) |
|
|
Quote:
Bobabouy said: But c10h12n2o said I could have an isolate in just a few transfers. 
He also has no ratings to verify his credibility, no grow logs, no longevity In the community and probably no basis at all from which to give cultivation advice
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
|
Bobabouy
Shrimp Boat Captain



Registered: 01/19/16
Posts: 661
Loc: The Black Sea
Last seen: 6 years, 16 days
|
|
And he also has no isolates.
|
Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 1 day, 8 hours
|
Re: To clone or to islotate. [Re: Bobabouy]
#23466198 - 07/22/16 02:14 PM (7 years, 6 months ago) |
|
|
Also I think you are misunderstanding what was said...,.it is said that isolates take hundreds of transfer to achieve because typically you will narrow down to have a nice selection of monocultures to test out to find your favorite.ll.lif you are just going to pick a strain to isolate with no regard or knowledge of its genetics and fruiting potential you can probably have an isolate after 4-5 transfers
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
|
Bobabouy
Shrimp Boat Captain



Registered: 01/19/16
Posts: 661
Loc: The Black Sea
Last seen: 6 years, 16 days
|
|
You just won't let me make fun of the guy will you?
|
Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
|
Re: To clone or to islotate. [Re: Bobabouy]
#23466219 - 07/22/16 02:19 PM (7 years, 6 months ago) |
|
|
|
Bobabouy
Shrimp Boat Captain



Registered: 01/19/16
Posts: 661
Loc: The Black Sea
Last seen: 6 years, 16 days
|
|
|
lukehighwalker710
Cloud 9 Dweller



Registered: 03/04/16
Posts: 609
Loc: out there
Last seen: 6 years, 22 days
|
Re: To clone or to islotate. [Re: Bobabouy]
#23466277 - 07/22/16 02:32 PM (7 years, 6 months ago) |
|
|
--------------------
Highwalker
|
Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 1 day, 8 hours
|
Re: To clone or to islotate. [Re: Bobabouy]
#23466294 - 07/22/16 02:36 PM (7 years, 6 months ago) |
|
|
Here's a little pictorial for you:
Select a mature fruit to clone:

In a SAB use your hands to pull the cap in half and then use a sterilized knife to cut a piece of cap flesh out and put it on agar and let it colonize:
>>>
Select good looking mycelium from initial clone plate and transfer:

Colonize and repeat until sectoring occurs:

Select mycelium no from individual sectors, and transfer....repeat to expose the monocultures:

Use to prepare an inoculant and grow your cloned isolate:
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
|
bloodwater
Stranger
Registered: 06/28/16
Posts: 21
Last seen: 7 years, 6 months
|
|
Right now I have some fully colonized plates. They are wrapped in 2-3 layers of parafilm. Should I lower it to 1-2? (I do this since I see tears a lot in my film and this greatly reduces side contamination.
|
Bobabouy
Shrimp Boat Captain



Registered: 01/19/16
Posts: 661
Loc: The Black Sea
Last seen: 6 years, 16 days
|
Re: To clone or to islotate. [Re: bloodwater]
#23466347 - 07/22/16 02:56 PM (7 years, 6 months ago) |
|
|
FFS one layer is fine! Hell, I only use plastic wrap.
|
bloodwater
Stranger
Registered: 06/28/16
Posts: 21
Last seen: 7 years, 6 months
|
Re: To clone or to islotate. [Re: Bobabouy]
#23466399 - 07/22/16 03:15 PM (7 years, 6 months ago) |
|
|
Parafilm tears and doesn't store well. When I did one layer I could visually see holes in it after a week.
PLastic wrap is maybe great for short term storage but doesn't cling well to dishes.
|
Bobabouy
Shrimp Boat Captain



Registered: 01/19/16
Posts: 661
Loc: The Black Sea
Last seen: 6 years, 16 days
|
Re: To clone or to islotate. [Re: bloodwater]
#23466405 - 07/22/16 03:17 PM (7 years, 6 months ago) |
|
|
I use "clingwrap" and yes it most definitely "clings". Why do you need to store it for so long? Even ones I keep in the fridge for a while are clingwrapped. Trust me, your throwing away money on parafilm.
Edited by Bobabouy (07/22/16 03:18 PM)
|
spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
|
|
but that's not a true isolate mycologist217, we were talking about spores to agar, 3-5 transfers and having an isolate from that.
edit, I'm not really convinced you have an isolated culture there
Edited by spacechildo (07/22/16 03:37 PM)
|
spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
|
Re: To clone or to islotate. [Re: bloodwater]
#23466435 - 07/22/16 03:26 PM (7 years, 6 months ago) |
|
|
Quote:
bloodwater said: Parafilm tears and doesn't store well. When I did one layer I could visually see holes in it after a week.
PLastic wrap is maybe great for short term storage but doesn't cling well to dishes.
you gotta store parafilm air tight or else it dries up and cracks as you've seen.
|
bloodwater
Stranger
Registered: 06/28/16
Posts: 21
Last seen: 7 years, 6 months
|
Re: To clone or to islotate. [Re: spacechildo]
#23466472 - 07/22/16 03:38 PM (7 years, 6 months ago) |
|
|
Spacechildo so would you recommend clingwrap over parafilm? Also I have a minifridge just for cultures
|
Bobabouy
Shrimp Boat Captain



Registered: 01/19/16
Posts: 661
Loc: The Black Sea
Last seen: 6 years, 16 days
|
Re: To clone or to islotate. [Re: bloodwater]
#23466493 - 07/22/16 03:45 PM (7 years, 6 months ago) |
|
|
Quote:
bloodwater said: Spacechildo so would you recommend clingwrap over parafilm? Also I have a minifridge just for cultures
What am I, chopped liver?
|
spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
|
Re: To clone or to islotate. [Re: Bobabouy]
#23466508 - 07/22/16 03:49 PM (7 years, 6 months ago) |
|
|
I'm not the best to ask, I dont do petris anymore as they're so damn expensive to ship. Both works, find your own preference! and parafilm can stretch a LOT.
|
Bobabouy
Shrimp Boat Captain



Registered: 01/19/16
Posts: 661
Loc: The Black Sea
Last seen: 6 years, 16 days
|
Re: To clone or to islotate. [Re: spacechildo]
#23466527 - 07/22/16 03:53 PM (7 years, 6 months ago) |
|
|
Just consider that clingwrap was mad for leftovers. Leftovers that stay in the Fridge! And it stretches and clings. You have my word on that. Take a super sharp knife and cut off a 1 - 1.25" section. A hole role will last you years. Just circumvent each plate 1.5 to 3 times while slightly stretched. Draw your own conclusion. It's cheap!
Edited by Bobabouy (07/22/16 03:55 PM)
|
bloodwater
Stranger
Registered: 06/28/16
Posts: 21
Last seen: 7 years, 6 months
|
Re: To clone or to islotate. [Re: Bobabouy]
#23466552 - 07/22/16 04:01 PM (7 years, 6 months ago) |
|
|
When I used cling wrap it would stick to my gloves and was not sticking to the dish tiself bobabouy that was also why I wanted a 2nd opion.
I ended up having a mess of uneven dishes due to me wadding up the cling wrap on the bottom of the dish.
|
Bobabouy
Shrimp Boat Captain



Registered: 01/19/16
Posts: 661
Loc: The Black Sea
Last seen: 6 years, 16 days
|
Re: To clone or to islotate. [Re: bloodwater]
#23466570 - 07/22/16 04:05 PM (7 years, 6 months ago) |
|
|
The only explanation I have for that is you were not using Clingwrap. Clingwrap is not the same thing as plastic/shrink wrap. It has to say " Clingwrap" on the box. Mad by Glad. "Search it out and yee shall be satisfied." That was a creepy voice btw.
Edited by Bobabouy (07/22/16 04:08 PM)
|
bloodwater
Stranger
Registered: 06/28/16
Posts: 21
Last seen: 7 years, 6 months
|
Re: To clone or to islotate. [Re: Bobabouy]
#23466605 - 07/22/16 04:14 PM (7 years, 6 months ago) |
|
|
white glaze, sticks to everything including your nitrite gloves, doesn't strech, and uneven texture?
Been a few months but it was something like that and said glad on it. I was trying to strech it around the dish lol.I would check back in a few weeks and my dishes would be dried out due to too much air exchange.
|
Bobabouy
Shrimp Boat Captain



Registered: 01/19/16
Posts: 661
Loc: The Black Sea
Last seen: 6 years, 16 days
|
Re: To clone or to islotate. [Re: bloodwater]
#23466618 - 07/22/16 04:17 PM (7 years, 6 months ago) |
|
|
OK brother. Say it one more time. Brand name: Clingwrap by Glad. Cut you off a little role from the end: 1-1.25 inches. WRAP! It does not stick to my gloves. And when it does, it's in a good way. Like when I'm trying to grab that little piece at the start of the role.
|
weetsie
unlicensed tub surgeon



Registered: 05/08/11
Posts: 572
Loc: United Kingdom
|
Re: To clone or to islotate. [Re: Bobabouy]
#23466670 - 07/22/16 04:35 PM (7 years, 6 months ago) |
|
|
It's called clingfilm in the UK, probably 95% of households have a roll of it in the kitchen. It doesn't stick to anything but itself, nitrile gloves are no exception, I just tested it. In the USA you will often find other types of plastic food wrap, translucent stuff that has some kind of mild adhesive on one side.
-------------------- Active grow logs: Oysters on Straw Pellets Trade list
|
bloodwater
Stranger
Registered: 06/28/16
Posts: 21
Last seen: 7 years, 6 months
|
Re: To clone or to islotate. [Re: weetsie]
#23467788 - 07/22/16 10:49 PM (7 years, 6 months ago) |
|
|
yeah I was using the wrong stuff before.
I am still a little iffy on cling route.
|
Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
|
Re: To clone or to islotate. [Re: bloodwater]
#23467858 - 07/22/16 11:11 PM (7 years, 6 months ago) |
|
|
Parafilm is pretty cool, you don't have to mess with it cutting the roll and shit so I prefer it. If your parafilm is tearing it's probably because it spent a lot of time stored at your supplier's. The two rolls of parafilm I have both do this.
If your parafilm keeps tearing, try letting it rest after stretching. What I do is I stretch, let it contract and then wrap it around the plate, stretching just enough for it not to have creases. Then I repeat until I wrap my piece all around. Some still tear, old parafilm seems to do this no matter what you do but it does take longer for it to tear and it does it less often.
They should really start doing a date of manufacture and best before date on parafilm, time certainly does have an effect on it's quality.
Those who say that parafilm is expensive probably never really bought a roll. Sure, it's more expensive than a roll of cling wrap but I've been using my roll for almost a year and I used about 1/2-3/4 of it and I go through a lot of agar. I inoculate exclusively with wedges so I probably use more agar than most for inoculation alone. I find it comfortable that you just buy a roll and cut off whatever you need rather than to mess with cutting a roll into smaller rolls or whatever the fuck you do with cling wrap. It's also specifically made for the job.
|
bloodwater
Stranger
Registered: 06/28/16
Posts: 21
Last seen: 7 years, 6 months
|
|
I left a few plates out a few weeks ago. I forgot about them and one has a pin on it. Going to transfer it tonight. These plates had some contamination. I am getting ready for it.
|
Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
|
Re: To clone or to islotate. [Re: bloodwater]
#23467890 - 07/22/16 11:22 PM (7 years, 6 months ago) |
|
|
See? You already have your first clone.
Transfer it, clean it up if neccessary, slant it (or put on an extra plate and cold store it) and grow it to see if it's worth keeping. Always have a plate or slant in cold storage before you do anything with it, just in case it's a keeper.
|
bloodwater
Stranger
Registered: 06/28/16
Posts: 21
Last seen: 7 years, 6 months
|
|
still no one has told mee if too much parafilm would stop invitro pins. I also transferred one from a dish today that I had laying around outside of the fridge.
|
Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
|
Re: To clone or to islotate. [Re: bloodwater]
#23468406 - 07/23/16 06:44 AM (7 years, 6 months ago) |
|
|
No, parafilm is breatheable. An agar plate requires no GE anyway. It probably speeds up colonization but the mycelium can still colonize the whole plate and pin without GE.
|
weetsie
unlicensed tub surgeon



Registered: 05/08/11
Posts: 572
Loc: United Kingdom
|
|
Quote:
Supalemonhaze said: No, parafilm is breatheable. An agar plate requires no GE anyway. It probably speeds up colonization but the mycelium can still colonize the whole plate and pin without GE.
those little tubs you use for agar then, you just clip the lids down, no modifications?
nvm just saw your sig after posting
-------------------- Active grow logs: Oysters on Straw Pellets Trade list
|
|