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Offlinebloodwater
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Registered: 06/28/16
Posts: 21
Last seen: 7 years, 6 months
Re: To clone or to islotate. [Re: spacechildo]
    #23466472 - 07/22/16 03:38 PM (7 years, 6 months ago)

Spacechildo so would you recommend clingwrap over parafilm? Also I have a minifridge just for cultures


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OfflineBobabouy
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Re: To clone or to islotate. [Re: bloodwater]
    #23466493 - 07/22/16 03:45 PM (7 years, 6 months ago)

Quote:

bloodwater said:
Spacechildo so would you recommend clingwrap over parafilm? Also I have a minifridge just for cultures



What am I, chopped liver?


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Offlinespacechildo
proletarians rise up
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Registered: 01/24/13
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Re: To clone or to islotate. [Re: Bobabouy]
    #23466508 - 07/22/16 03:49 PM (7 years, 6 months ago)

I'm not the best to ask, I dont do petris anymore as they're so damn expensive to ship.
Both works, find your own preference! and parafilm can stretch a LOT.


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OfflineBobabouy
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Re: To clone or to islotate. [Re: spacechildo]
    #23466527 - 07/22/16 03:53 PM (7 years, 6 months ago)

Just consider that clingwrap was mad for leftovers. Leftovers that stay in the Fridge! And it stretches and clings. You have my word on that. Take a super sharp knife and cut off a 1 - 1.25" section. A hole role will last you years. Just circumvent each plate 1.5 to 3 times while slightly stretched. Draw your own conclusion. It's cheap!


Edited by Bobabouy (07/22/16 03:55 PM)


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Offlinebloodwater
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Re: To clone or to islotate. [Re: Bobabouy]
    #23466552 - 07/22/16 04:01 PM (7 years, 6 months ago)

When I used cling wrap it would stick to my gloves and was not sticking to the dish tiself bobabouy that was also why I wanted a 2nd opion.

I ended up having a mess of uneven dishes due to me wadding up the cling wrap on the bottom of the dish.


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OfflineBobabouy
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Re: To clone or to islotate. [Re: bloodwater]
    #23466570 - 07/22/16 04:05 PM (7 years, 6 months ago)

The only explanation I have for that is you were not using Clingwrap. Clingwrap is not the same thing as plastic/shrink wrap. It has to say "
Clingwrap" on the box. Mad by Glad. "Search it out and yee shall be satisfied." That was a creepy voice btw.


Edited by Bobabouy (07/22/16 04:08 PM)


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Offlinebloodwater
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Re: To clone or to islotate. [Re: Bobabouy]
    #23466605 - 07/22/16 04:14 PM (7 years, 6 months ago)

white glaze, sticks to everything including your nitrite gloves, doesn't strech, and uneven texture?

Been a few months but it was something like that and said glad on it. I was trying to strech it around the dish lol.I would check back in a few weeks and my dishes would be dried out due to too much air exchange.


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OfflineBobabouy
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Re: To clone or to islotate. [Re: bloodwater]
    #23466618 - 07/22/16 04:17 PM (7 years, 6 months ago)

OK brother. Say it one more time. Brand name: Clingwrap by Glad. Cut you off a little role from the end: 1-1.25 inches. WRAP! It does not stick to my gloves. And when it does, it's in a good way. Like when I'm trying to grab that little piece at the start of the role.


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Invisibleweetsie
unlicensed tub surgeon
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Registered: 05/08/11
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Re: To clone or to islotate. [Re: Bobabouy]
    #23466670 - 07/22/16 04:35 PM (7 years, 6 months ago)

It's called clingfilm in the UK, probably 95% of households have a roll of it in the kitchen. It doesn't stick to anything but itself, nitrile gloves are no exception, I just tested it. In the USA you will often find other types of plastic food wrap, translucent stuff that has some kind of mild adhesive on one side.


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Offlinebloodwater
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Re: To clone or to islotate. [Re: weetsie]
    #23467788 - 07/22/16 10:49 PM (7 years, 6 months ago)

yeah I was using the wrong stuff before.

I am still a little iffy on cling route.


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InvisibleSupalemonhaze
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Registered: 10/02/15
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Loc: 12" down Europe's butthole
Re: To clone or to islotate. [Re: bloodwater]
    #23467858 - 07/22/16 11:11 PM (7 years, 6 months ago)

Parafilm is pretty cool, you don't have to mess with it cutting the roll and shit so I prefer it. If your parafilm is tearing it's probably because it spent a lot of time stored at your supplier's. The two rolls of parafilm I have both do this.

If your parafilm keeps tearing, try letting it rest after stretching. What I do is I stretch, let it contract and then wrap it around the plate, stretching just enough for it not to have creases. Then I repeat until I wrap my piece all around. Some still tear, old parafilm seems to do this no matter what you do but it does take longer for it to tear and it does it less often.

They should really start doing a date of manufacture and best before date on parafilm, time certainly does have an effect on it's quality.

Those who say that parafilm is expensive probably never really bought a roll. Sure, it's more expensive than a roll of cling wrap but I've been using my roll for almost a year and I used about 1/2-3/4 of it and I go through a lot of agar. I inoculate exclusively with wedges so I probably use more agar than most for inoculation alone. I find it comfortable that you just buy a roll and cut off whatever you need rather than to mess with cutting a roll into smaller rolls or whatever the fuck you do with cling wrap. It's also specifically made for the job.


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Offlinebloodwater
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Re: To clone or to islotate. [Re: Supalemonhaze]
    #23467871 - 07/22/16 11:15 PM (7 years, 6 months ago)

I left a few plates out a few weeks ago. I forgot about them and one has a pin on it. Going to transfer it tonight. These plates had some contamination. I am getting ready for it.


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InvisibleSupalemonhaze
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Registered: 10/02/15
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Loc: 12" down Europe's butthole
Re: To clone or to islotate. [Re: bloodwater]
    #23467890 - 07/22/16 11:22 PM (7 years, 6 months ago)

See? You already have your first clone.

Transfer it, clean it up if neccessary, slant it (or put on an extra plate and cold store it) and grow it to see if it's worth keeping. Always have a plate or slant in cold storage before you do anything with it, just in case it's a keeper.


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Offlinebloodwater
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Re: To clone or to islotate. [Re: Supalemonhaze]
    #23468316 - 07/23/16 04:16 AM (7 years, 6 months ago)

still no one has told mee if too much parafilm would stop invitro pins. I also transferred one from a dish today that I had laying around outside of the fridge.


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InvisibleSupalemonhaze
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Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: To clone or to islotate. [Re: bloodwater]
    #23468406 - 07/23/16 06:44 AM (7 years, 6 months ago)

No, parafilm is breatheable. An agar plate requires no GE anyway. It probably speeds up colonization but the mycelium can still colonize the whole plate and pin without GE.


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Invisibleweetsie
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Registered: 05/08/11
Posts: 572
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Re: To clone or to islotate. [Re: Supalemonhaze]
    #23468773 - 07/23/16 10:01 AM (7 years, 6 months ago)

Quote:

Supalemonhaze said:
No, parafilm is breatheable. An agar plate requires no GE anyway. It probably speeds up colonization but the mycelium can still colonize the whole plate and pin without GE.




those little tubs you use for agar then, you just clip the lids down, no modifications?

nvm just saw your sig after posting :thumbup:


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