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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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Re: To clone or to islotate. [Re: Pastywhyte] 1
#23465944 - 07/22/16 12:42 PM (7 years, 6 months ago) |
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Quote:
Pastywhyte said: Don't forget that invitro pins often mean they can grow with shit FAE and little water or nutes. Once you get them on a nutrition and water rich sub in decent FAE they often really go nuts. My best clones are all from invitro pins and I have yet to see anyone throw up an isolate that can compete with my high producers pound for pound.
this is kinda what i was thinking(and hoping) might be the case for the clones i have taken from plates.
and OP, in this context, the in-vitro pin refers to a pin that is pinning on an agar plate. that way you dont have to actually fruit anything to see if it will fruit.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: To clone or to islotate. [Re: Pastywhyte]
#23465960 - 07/22/16 12:47 PM (7 years, 6 months ago) |
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Quote:
Pastywhyte said: Don't forget that invitro pins often mean they can grow with shit FAE and little water or nutes. Once you get them on a nutrition and water rich sub in decent FAE they often really go nuts. My best clones are all from invitro pins and I have yet to see anyone throw up an isolate that can compete with my high producers pound for pound.
Or they can turn out to be mad pinners but buzzkill growers like the ones we were talking about in the other thread 
When it comes to cloning, I think one should do both. Cloning a mature fruit will have a better chance of turning into what you wanted it to look like but invitro clones are easy to get, just neglect a plate and clone it once it grows. I like to leave a plate of each new variety I get solely for invitro pins.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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I usually look for invitro pins that pop up right away. Pins that show after the plate sits around for 3 months are usually not worth my time.
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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Re: To clone or to islotate. [Re: Pastywhyte]
#23465974 - 07/22/16 12:53 PM (7 years, 6 months ago) |
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thats what i did. after i transfer i let my palte sit for a couple weeks. if it clones soon after it finished colonizing the plate, then ill take it, and if not after a week or two after it colonizes the whole plate, i toss it.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: To clone or to islotate. [Re: Pastywhyte]
#23466116 - 07/22/16 01:51 PM (7 years, 6 months ago) |
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Quote:
Pastywhyte said: I usually look for invitro pins that pop up right away. Pins that show after the plate sits around for 3 months are usually not worth my time.
Well that is disheartening, my one and only PE invitro pin took months to form. The only one that pinned within weeks for me was martinique, that one is quite fierce. I'm still waiting for my AA+, F+ and GT to pin but I think they have been there for at least 3-4weeks.
Would you say the amount of nutrients has an effect on how fast invitro pins form? I use a nutrient poor MEA recipe almost exclusively.
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Bobabouy
Shrimp Boat Captain



Registered: 01/19/16
Posts: 661
Loc: The Black Sea
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But c10h12n2o said I could have an isolate in just a few transfers.
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Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
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Re: To clone or to islotate. [Re: Bobabouy]
#23466171 - 07/22/16 02:08 PM (7 years, 6 months ago) |
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Quote:
Bobabouy said: But c10h12n2o said I could have an isolate in just a few transfers. 
He also has no ratings to verify his credibility, no grow logs, no longevity In the community and probably no basis at all from which to give cultivation advice
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
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Bobabouy
Shrimp Boat Captain



Registered: 01/19/16
Posts: 661
Loc: The Black Sea
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And he also has no isolates.
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Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
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Re: To clone or to islotate. [Re: Bobabouy]
#23466198 - 07/22/16 02:14 PM (7 years, 6 months ago) |
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Also I think you are misunderstanding what was said...,.it is said that isolates take hundreds of transfer to achieve because typically you will narrow down to have a nice selection of monocultures to test out to find your favorite.ll.lif you are just going to pick a strain to isolate with no regard or knowledge of its genetics and fruiting potential you can probably have an isolate after 4-5 transfers
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
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Bobabouy
Shrimp Boat Captain



Registered: 01/19/16
Posts: 661
Loc: The Black Sea
Last seen: 6 years, 16 days
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You just won't let me make fun of the guy will you?
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: To clone or to islotate. [Re: Bobabouy]
#23466219 - 07/22/16 02:19 PM (7 years, 6 months ago) |
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Bobabouy
Shrimp Boat Captain



Registered: 01/19/16
Posts: 661
Loc: The Black Sea
Last seen: 6 years, 16 days
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lukehighwalker710
Cloud 9 Dweller



Registered: 03/04/16
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Re: To clone or to islotate. [Re: Bobabouy]
#23466277 - 07/22/16 02:32 PM (7 years, 6 months ago) |
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--------------------
Highwalker
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Mycologist217
Frank's Disciple



Registered: 02/13/13
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Re: To clone or to islotate. [Re: Bobabouy]
#23466294 - 07/22/16 02:36 PM (7 years, 6 months ago) |
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Here's a little pictorial for you:
Select a mature fruit to clone:

In a SAB use your hands to pull the cap in half and then use a sterilized knife to cut a piece of cap flesh out and put it on agar and let it colonize:
>>>
Select good looking mycelium from initial clone plate and transfer:

Colonize and repeat until sectoring occurs:

Select mycelium no from individual sectors, and transfer....repeat to expose the monocultures:

Use to prepare an inoculant and grow your cloned isolate:
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
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bloodwater
Stranger
Registered: 06/28/16
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Last seen: 7 years, 6 months
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Right now I have some fully colonized plates. They are wrapped in 2-3 layers of parafilm. Should I lower it to 1-2? (I do this since I see tears a lot in my film and this greatly reduces side contamination.
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Bobabouy
Shrimp Boat Captain



Registered: 01/19/16
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Re: To clone or to islotate. [Re: bloodwater]
#23466347 - 07/22/16 02:56 PM (7 years, 6 months ago) |
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FFS one layer is fine! Hell, I only use plastic wrap.
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bloodwater
Stranger
Registered: 06/28/16
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Re: To clone or to islotate. [Re: Bobabouy]
#23466399 - 07/22/16 03:15 PM (7 years, 6 months ago) |
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Parafilm tears and doesn't store well. When I did one layer I could visually see holes in it after a week.
PLastic wrap is maybe great for short term storage but doesn't cling well to dishes.
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Bobabouy
Shrimp Boat Captain



Registered: 01/19/16
Posts: 661
Loc: The Black Sea
Last seen: 6 years, 16 days
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Re: To clone or to islotate. [Re: bloodwater]
#23466405 - 07/22/16 03:17 PM (7 years, 6 months ago) |
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I use "clingwrap" and yes it most definitely "clings". Why do you need to store it for so long? Even ones I keep in the fridge for a while are clingwrapped. Trust me, your throwing away money on parafilm.
Edited by Bobabouy (07/22/16 03:18 PM)
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
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but that's not a true isolate mycologist217, we were talking about spores to agar, 3-5 transfers and having an isolate from that.
edit, I'm not really convinced you have an isolated culture there
Edited by spacechildo (07/22/16 03:37 PM)
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spacechildo
proletarians rise up


Registered: 01/24/13
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Re: To clone or to islotate. [Re: bloodwater]
#23466435 - 07/22/16 03:26 PM (7 years, 6 months ago) |
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Quote:
bloodwater said: Parafilm tears and doesn't store well. When I did one layer I could visually see holes in it after a week.
PLastic wrap is maybe great for short term storage but doesn't cling well to dishes.
you gotta store parafilm air tight or else it dries up and cracks as you've seen.
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