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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 5 months
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Re: Strain Isolations on Agar, Pics and Questions [Re: Boogieman47]
#23463094 - 07/21/16 04:10 PM (7 years, 7 months ago) |
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ya little quote fuck up there.. but you did make me !
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: Strain Isolations on Agar, Pics and Questions [Re: spacechildo]
#23463106 - 07/21/16 04:13 PM (7 years, 7 months ago) |
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Haha oh shit I didn't even realize that well I was up late as Fuck that makes "since" to me now Lmao
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spacechildo
proletarians rise up


Registered: 01/24/13
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Re: Strain Isolations on Agar, Pics and Questions [Re: Boogieman47]
#23463135 - 07/21/16 04:23 PM (7 years, 7 months ago) |
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Rooster Cogburn
Ranger

Registered: 07/19/16
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Re: Strain Isolations on Agar, Pics and Questions [Re: spacechildo]
#23463259 - 07/21/16 05:02 PM (7 years, 7 months ago) |
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Ok, I see what you mean. As far as published work go's what do you feel is the most up to date piece of work concerning mushroom cultivation besides TMC or GGMM?
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spacechildo
proletarians rise up


Registered: 01/24/13
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Re: Strain Isolations on Agar, Pics and Questions [Re: Rooster Cogburn] 1
#23463286 - 07/21/16 05:15 PM (7 years, 7 months ago) |
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shroomery.org
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MysticMoteToter



Registered: 07/26/15
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Re: Strain Isolations on Agar, Pics and Questions [Re: Rooster Cogburn]
#23463384 - 07/21/16 05:40 PM (7 years, 7 months ago) |
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Quote:
Rooster Cogburn said: Ok, I see what you mean. As far as published work go's what do you feel is the most up to date piece of work concerning mushroom cultivation besides TMC or GGMM?
https://www.shroomery.org/forums/search.php this is a good way to refine what your looking for, but published work on the cultivation of actives will be very hard to come by due to drug scheduling laws haha
-------------------- Half Homo Hardly Sapient Overview Effect Fuck War, Feed Birds.
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 5 months
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Re: Strain Isolations on Agar, Pics and Questions [Re: MysticMoteToter]
#23463469 - 07/21/16 06:05 PM (7 years, 7 months ago) |
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all the good regulars here arent getting published but their writeups are way way better than most "published work" we get linked here on a weekly basis  I bet they're good lab technicians, but they dont know jack shit about mushrooms, growing MS taking notes thinking they found the holy grail/answer to 43.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
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Re: Strain Isolations on Agar, Pics and Questions [Re: spacechildo]
#23463765 - 07/21/16 07:39 PM (7 years, 7 months ago) |
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I barely g2g, there's other reliable methods to get things done quickly. G2G for me usually ends up with signs of bacteria so I only do it with stuff that's gonna be fruited outside, like oysters.
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Rooster Cogburn
Ranger

Registered: 07/19/16
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Re: Strain Isolations on Agar, Pics and Questions [Re: Supalemonhaze]
#23464300 - 07/21/16 10:36 PM (7 years, 7 months ago) |
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How do you think you are getting bacteria during your G2G transfers, are you using jars or bags?. Personally I like going from my master jars straight to bags, use some bags for tubs and take another bag to bunch more bags and so on about 5 generations, usually 8 lb. or 8 quart bags, I have found that to be the fastest method for me so far. What do you prefer to do other than G2G?
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
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Loc: 12" down Europe's butthole
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Re: Strain Isolations on Agar, Pics and Questions [Re: Rooster Cogburn]
#23464703 - 07/22/16 01:13 AM (7 years, 7 months ago) |
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If I wanted anything done fast, I would go with LI.
I think the main problem with me G2Ging is that the endospores would have recovered by the time my jars are colonized. G2Ging will just give those endospores grains to grow on. I can have a very healthy looking jar, G2G it and then see definite signs of bacteria. No stalling or anything but I get grains stuck to sides/thick mycelium etc. All spawnable but the point to using agar is to get the cleanest spawn possible, G2G's basically fucks up all that hard work.
For inside grows, I stick to wedges. Speed is not an issue once you start inoculating jars with a routine, you'll always have something to spawn when you start doing that.
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
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Re: Strain Isolations on Agar, Pics and Questions [Re: Supalemonhaze]
#23464752 - 07/22/16 01:45 AM (7 years, 7 months ago) |
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I've noticed that too when I do g2g the spawn sometimes comes back fucked up I just thought my sterile technique was sloppy
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spacechildo
proletarians rise up


Registered: 01/24/13
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Re: Strain Isolations on Agar, Pics and Questions [Re: Supalemonhaze]
#23465263 - 07/22/16 08:52 AM (7 years, 7 months ago) |
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sterilize your master jars longer supa.
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Strain Isolations on Agar, Pics and Questions [Re: spacechildo]
#23465283 - 07/22/16 08:59 AM (7 years, 7 months ago) |
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I had a similar problem, but mine was because i sucked at g2g at first and would get mold in the receivers too much, so i switched to master only.
pasty turned me onto pint masters. i cook them with qt jars, so they get sterilized a while for the amount of grain in them and they colonize pretty quick from wedge. ill go to 2-3 qts after that.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Strain Isolations on Agar, Pics and Questions [Re: spacechildo]
#23465382 - 07/22/16 09:29 AM (7 years, 7 months ago) |
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Quote:
spacechildo said: sterilize your master jars longer supa.
I PC for a good amount, or so I think at least. I PC my jars for a total of 2hr15m. Used to do 2hrs before that but a few weeks ago I timed how long it takes for the core to get completely saturated with heat. It took 45 minutes so with 2hr15m the jars spend 1hr30m at ~121F. Isn't going over this overkill? I always thought it was but IDK really.
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
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Re: Strain Isolations on Agar, Pics and Questions [Re: Supalemonhaze]
#23465432 - 07/22/16 09:42 AM (7 years, 7 months ago) |
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must be your sterile tech then
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Strain Isolations on Agar, Pics and Questions [Re: spacechildo]
#23465464 - 07/22/16 09:54 AM (7 years, 7 months ago) |
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Edit:
Unless you're serious... In which case... I figure you're joking though.
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spacechildo
proletarians rise up


Registered: 01/24/13
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Re: Strain Isolations on Agar, Pics and Questions [Re: Supalemonhaze]
#23465482 - 07/22/16 09:59 AM (7 years, 7 months ago) |
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well OK you're right g2g doesnt work
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
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Re: Strain Isolations on Agar, Pics and Questions [Re: spacechildo]
#23465491 - 07/22/16 10:02 AM (7 years, 7 months ago) |
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Oh, I guess you were serious then. Inadequate sterile tek normally results in mold contams, not bacteria. For me at least. I reckon something else is going on, endospores is the only thing I can think of. Or maybe I'm crap at spotting minute bacteria contams. The former makes more sense to me because it's unlikely for me to have invisible bacteria in all of my plates.
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 5 months
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Re: Strain Isolations on Agar, Pics and Questions [Re: Supalemonhaze]
#23465593 - 07/22/16 10:39 AM (7 years, 7 months ago) |
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you should never stop striving for better sterile tech IMO. And I know g2g works great so it shouldn't be a problem for you
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Strain Isolations on Agar, Pics and Questions [Re: spacechildo]
#23465650 - 07/22/16 11:11 AM (7 years, 7 months ago) |
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But how can you say it's the sterile tek when none of the jars are getting molds? Don't get me wrong, my sterile tek is far from bulletproof and even if it was, you're always learning. I just don't think that if I g2ged 9 recievers and I got bacteria in every single one, it's sterile tek related.
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