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InvisiblePsychedelic Pupil
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Plate Count Agar for spores and clones?
    #23462600 - 07/21/16 12:56 PM (7 years, 7 months ago)

I know it's inexpensive to make my own agar mixtures but I have free access to BD Difco Dehydrated Culture Media PCA. It's specs are: Pancreatic digest of Casein 5g/L, Yeast extract 2.5g/L, Dextrose 1g/L, Agar 15g/L

Has anyone used this? It seems like the Casein might invite problems. Do you think it's worth giving this a shot for cleaning up wild oyster spores or cloning foraged mushrooms or should I not waste my time?

Thanks!


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OfflineGr0wer
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Re: Plate Count Agar for spores and clones? [Re: Psychedelic Pupil]
    #23462616 - 07/21/16 01:01 PM (7 years, 7 months ago)

Give it a shot, i just got some odball dishes like that from a lab buddy and it worked fine for oysters.

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Offlinedrake89
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Re: Plate Count Agar for spores and clones? [Re: Gr0wer]
    #23462671 - 07/21/16 01:25 PM (7 years, 7 months ago)

If it's made for bacteria then it may not be ideal.  I dunno if casein digest is a carbohydrate or not.  I think it's just protein.


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InvisiblePsychedelic Pupil
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Re: Plate Count Agar for spores and clones? [Re: drake89]
    #23462729 - 07/21/16 01:58 PM (7 years, 7 months ago)

I think that's exactly what it's for. It's from a waste water testing lab.

It's not going to cost me anything but a bit of time to try it. Hey, maybe it will be a good test of my sterile procedures if it's more like to grow bacteria :smile:


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Offlinedrake89
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Re: Plate Count Agar for spores and clones? [Re: Psychedelic Pupil]
    #23462809 - 07/21/16 02:36 PM (7 years, 7 months ago)

Yeah give it a shot.  Mycelium will grow on water and agar so just one transfer should be fine.


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