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Dare2d
Dare

Registered: 05/24/16
Posts: 22
Last seen: 2 years, 4 months
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Liquid culture (Pics)
#23462525 - 07/21/16 12:14 PM (7 years, 6 months ago) |
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So these LC (honey water + Spore syringe) has been in the works for 3 weeks now. Seeing some myc in there but need to double check with you guys on its health. Looks healthy & smells neutral. But what do you guys think ?
 

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lukehighwalker710
Cloud 9 Dweller



Registered: 03/04/16
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Re: Liquid culture (Pics) [Re: Dare2d]
#23462532 - 07/21/16 12:18 PM (7 years, 6 months ago) |
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The only way to know if an LC is clean is to test it it could be hiding tons of nasties or it could be a healthy culture but you can't see with just your eyes and we definitely can't with some pics. Spore syringe to LC is very risky and more likely to fail than work. Agar should be used to start an LC. However, Put a drop to some agar plates and see what grows. But... Are those in a drinking glass with a tinfoil lid?? Were they PCd?
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Highwalker
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Psilosoulful

Registered: 09/05/14
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Re: Liquid culture (Pics) [Re: Dare2d]
#23462537 - 07/21/16 12:21 PM (7 years, 6 months ago) |
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Don't go by looks. Don't go by smell. You need to test it out on a plate of agar to make sure it's clean, before using it on a grain jar. And being as it was started from a spore syringe, there's a very good chance that there is some level of bacteria present.
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Bobabouy
Shrimp Boat Captain



Registered: 01/19/16
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I would say your chances with that are less than zero. If that was possible.
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Dare2d
Dare

Registered: 05/24/16
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Thanks for the reply. Its drinking glass indeed. I know its not ideal. But thought of it at the last moment and gave it a go anyway. Yes its PCed.
Dont really have ready access to agar. Will try to procure some in the meanwhile. It would be good to experiment and learn more on LCs/Agar. :-)
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Bobabouy
Shrimp Boat Captain



Registered: 01/19/16
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Re: Liquid culture (Pics) [Re: Dare2d]
#23462566 - 07/21/16 12:41 PM (7 years, 6 months ago) |
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If you can get some grains going from your syringe you could use a single grain to make an LC. I still wouldn't recommend that and you will never succeed in a glass with foil. PC'd or not. Good luck.
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locallorax
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Registered: 07/27/12
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Quote:
lukehighwalker710 said: The only way to know if an LC is clean is to test it. Are those in a drinking glass with a tinfoil lid?? Were they PCd?
agreed
smell? is it not hermetically sealed? if not, it's probably contaminated and I wouldn't invest any more effort into it
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blackout


Registered: 07/16/00
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Re: Liquid culture (Pics) [Re: locallorax]
#23462605 - 07/21/16 12:59 PM (7 years, 6 months ago) |
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Quote:
locallorax said: smell? is it not hermetically sealed?
That's what I wondered, how did he manage to smell it.
If you were to test it I would draw it into a syringe and then ditch the LC and just use the same syringe full.
People were saying to test on LC. I had a recent thread about the possibility of selling extra clean syringes.
Quote:
Ryche Hawk said: I've used my syringes numerous times in LC's without problems but the solution should go on grain afterward, not agar.
Paul Stamets himself teaches spores will always have trace amounts of bacteria. When making spore syringes the water can help magnify that bacteria, but when inoculated in grain the mycelium easily out completes that bacteria so you don't see it. But when put on agar, the bacteria can spread faster then the mycelium. This is why I don't recommend spore syringe on agar, only use spore prints for agar and sector out any bacteria. Although I have seen times where no bacteria shows on agar from spore syringes I've just as many times it does. But those syringes that showed bacteria on agar when used on grain showed nothing but good healthy mycelium growing.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Liquid culture (Pics) [Re: blackout]
#23462662 - 07/21/16 01:22 PM (7 years, 6 months ago) |
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I have had syringes I knew were bacterial manage to colonize grain. I also had ones turn into a jar of nasty. Depends a lot on the populations in the syringe. There is definitely a tipping point where there is no return, especially if the spores take longer than a few days to germinate.
A few drops to a grain jar would be the best bet, then expand the good ones via g2g, but too often people fire in a full cc or more. That's giving the bacteria a real edge. Of course with LC it's even worse because motile bacteria can travel and expand in liquid faster than mycelium can and will have a headstart due to not really needing germinate.
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blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 2 months, 25 days
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Re: Liquid culture (Pics) [Re: Pastywhyte]
#23462723 - 07/21/16 01:55 PM (7 years, 6 months ago) |
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Quote:
Pastywhyte said: A few drops to a grain jar would be the best bet, then expand the good ones via g2g, but too often people fire in a full cc or more.
I have heard bacteria does not readily grow upwards, why people use that hot pour method of agar, pouring agar on top of a colonising plate and clean myc growing through it. I also head of putting dishes/jars on their side so the clean myc grows upwards. I wondered if the same idea could work with grains, for those who cannot easily get agar. Get a mini jar and put a small amount of grains in it, inject spores into the bottom and do not shake or anything. Then the myc colonises upwards, never shake the jar but carefully open it and pick off a few of the top grains and do a G2G with another jar.
So it is sort of like agar work in that you are transferring from the "leading edge".
I remember now Stamets did similar with tubes
Quote:
blackout said: I am not sure if I am allowed put the short (6 sentence) quote up? or even a few phrases/terminology?
In Stamets book "GROWING GOURMET and MEDICINAL MUSHROOMS" he speaks of growing myc through selective substrates in a tube. Like you might have mold & myc growing and put it into the top of a tube with a substrate which favours myc and is not liked by the mold growth. So it colonises through the substrate in the tube and you get "clean" myc out the top and can transfer it on.
Has anybody experience doing this, or suggest substrates for cubensis myc? I am guessing maybe sawdust or cardboard, as I hear cubensis will colonise wood though it is not a "wood lover"
I will stick up the quote if allowed as he says it far better than I ever could. Those who have the book its on page 101
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spacechildo
proletarians rise up


Registered: 01/24/13
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Re: Liquid culture (Pics) [Re: blackout]
#23462743 - 07/21/16 02:07 PM (7 years, 6 months ago) |
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Why should the LC go on grain and not agar afterwards? makes me real suspicious! if it doesnt show clean on agar how clean is it really?
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blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 2 months, 25 days
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Quote:
spacechildo said: Why should the LC go on grain and not agar afterwards? makes me real suspicious! if it doesnt show clean on agar how clean is it really?
Ryche Hawk appeared to be saying saying it is possibly clean enough to successfully grow shrooms with. Of course it might not be optimal growth, it could be wasting energy competing with contams.
He seemed to think it was very likely to show contams on agar, and I guess many who test on agar and see such contams would have presumed if it had gone to grains it would have been 100% sure to be a complete disaster.
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spacechildo
proletarians rise up


Registered: 01/24/13
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Re: Liquid culture (Pics) [Re: blackout]
#23462805 - 07/21/16 02:35 PM (7 years, 6 months ago) |
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To me, there's no point in making a LC unless you plan on noc'ing up a BUNCH of jars and dont wanna bother g2g.
If its not "really clean" or as clean as a perfectionist/serious grower would want it, it just doesnt make sense to me not to start a culture on agar to noc up the LC with.
LC's being noob friendly, saving spores as they say just dont make sense to me, want a lifetime supply of spores just get pf-tek right 1 time
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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If I wanted to stretch a spore syringe I would sterilize a half gallon of water and fire the vendor syringe into that. It would take a long time to use up and the CFU populations would be greatly diluted with more than enough spores to get the job done
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Liquid culture (Pics) [Re: Pastywhyte]
#23463015 - 07/21/16 03:44 PM (7 years, 6 months ago) |
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Too bad you werent here 20 yrs ago to impose that as the #1 noob way of stretching spores, damage already done and everyone new seems to think its 1cc to honey-water LC.
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