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Offlinenicelee808
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Can you mix spores
    #23462012 - 07/21/16 08:41 AM (7 years, 6 months ago)

I recently made some spore syringes from about 5 spores. Im not sure if they were the same mushroom breed.  At the time I was thinking the more the better. All the mushrooms were probably closely related as they were picked in the same area. All on cow dung. 1 was quite a bit larger than the others. Does anyone have any knowledge about this


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OfflinecronicrFacebook
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Re: Can you mix spores [Re: nicelee808]
    #23462022 - 07/21/16 08:44 AM (7 years, 6 months ago)

:facepalm: i would go find more fruits and start over


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Invisiblemupetmower
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Re: Can you mix spores [Re: nicelee808]
    #23462024 - 07/21/16 08:45 AM (7 years, 6 months ago)

wait.. so.. oh man.

so you made a spores syringe from a bunch of random mushrooms you found on cow shit? not knowing if they were even all cubes? there are so many thing wrong with this.

for one, you probably wont have any luck using that, because there is going to be a fuck-ton of bacteria and shit in it. if you inoc'd anything with that, it will likely fail.

secondly, you dont want to be growing random mushrooms, that you are planning to eat. you couldve gotten something poisonous without realizing.

you should do a LOT more reading and research on this, and then purchase a spore syringe from a vendor, or something, or trade for a print from someone here. but do the reading first, that way you know what you will need to be doing, and what supplies you will need.


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Offlinenicelee808
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Re: Can you mix spores [Re: mupetmower]
    #23462040 - 07/21/16 08:52 AM (7 years, 6 months ago)

Actually. Sorry. Maybe my original post was misleading. Im quite sure they were all cubes. And they worked well after i ate them. Maybe they might of been different breeds of cubes because of the sizes


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Invisiblemupetmower
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Re: Can you mix spores [Re: nicelee808]
    #23462042 - 07/21/16 08:53 AM (7 years, 6 months ago)

oh.. well still, that syringe will likely only lead do bacteria/contam/mold.


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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Offlinenicelee808
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Re: Can you mix spores [Re: mupetmower]
    #23462050 - 07/21/16 08:57 AM (7 years, 6 months ago)

The reason im sure they were cubes. Is because they all had the blue bruising on the stems


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OfflineYerow
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Re: Can you mix spores [Re: nicelee808]
    #23462053 - 07/21/16 08:57 AM (7 years, 6 months ago)

:doublefacepalm:


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Invisiblemupetmower
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Re: Can you mix spores [Re: nicelee808]
    #23462055 - 07/21/16 08:58 AM (7 years, 6 months ago)

while cubes do bruise blue, that can not 100% guarantee that they are cubes for certain.

but that is besides the point. if you wanna try inoc'ing some brf cakes with that syringe, then go for it. but just dont expect much more than contam and bacteria.


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-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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OfflinecronicrFacebook
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Re: Can you mix spores [Re: mupetmower]
    #23462078 - 07/21/16 09:07 AM (7 years, 6 months ago)

all cubes are cubes


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Offlinenicelee808
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Re: Can you mix spores [Re: cronicr]
    #23462130 - 07/21/16 09:27 AM (7 years, 6 months ago)

Just to be clear. I have been eating these shrooms for a while. Ive learnt to identify the difference between the magic and the non magic. As a safety precaution I only eat them if they have grown in cow dung or near. They have black spores and have blue bruising on the stem once handled.

As for the the inoculating what reason would they likely be contam. Because the mixing the spores or because of the poo.  The spores were stored in the fridge for some time and I used quite warm sterile water to make the syringe. Im not uf thats a good thing seemed like a good udea at the time.  My jars to have mycelium growing no sign of mold yet. Its been nearly 2 weeks


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Invisiblemupetmower
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Re: Can you mix spores [Re: nicelee808]
    #23462136 - 07/21/16 09:31 AM (7 years, 6 months ago)

because the spores were taken from wild prints that were not grown in sterile conditions. there is going to be a lot of bacteria and other crap in that syringe that will fuck up your grow. just putting those spores in sterile water isnt enough, because the spores themselves werent sterile. you still have bacteria and other contam in that syringe, now. if you want to use it, start with agar. put a drop of the solution on an agar plate, and make transfers to clean it up from the bacteria and other stuff. then you can use that to inoculate, knowing it is clean.

like i said, if you want to try using it, then go for it. just dont expect much of anything.


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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Offlinenicelee808
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Re: Can you mix spores [Re: mupetmower]
    #23462161 - 07/21/16 09:39 AM (7 years, 6 months ago)

Quote:

mupetmower said:
because the spores were taken from wild prints that were not grown in sterile conditions. there is going to be a lot of bacteria and other crap in that syringe that will fuck up your grow. just putting those spores in sterile water isnt enough, because the spores themselves werent sterile. you still have bacteria and other contam in that syringe, now. if you want to use it, start with agar. put a drop of the solution on an agar plate, and make transfers to clean it up from the bacteria and other stuff. then you can use that to inoculate, knowing it is clean.

like i said, if you want to try using it, then go for it. just dont expect much of anything.




Thanks for the info. Need to learn about agar


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Invisiblemupetmower
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Re: Can you mix spores [Re: nicelee808]
    #23462165 - 07/21/16 09:40 AM (7 years, 6 months ago)

Pasty Plates

Agar 123

Agar Portal

Those can get you started


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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OfflineEywa_devotee
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Re: Can you mix spores [Re: mupetmower]
    #23462906 - 07/21/16 03:09 PM (7 years, 6 months ago)

If you recently taken the prints and went straight to grain it's an almost guaranteed fail. You'll have better luck if you age the prints for 6 months or so, but from wild prints it's going to be a gamble. If you want to attempt to grow them now, use one of the above agar teks. Oh you can get agar from Asian and Indian grocery stores very cheaply BTW.


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"Love one another." "To Love is to know me." "Love is the Law, Love under Will." "In Compassion, all sorrows end." Regardless of the Master, the message is the same- Choose love and you shall live, Choose Fear and you shall die. Help bring peace to this Earth: Love one another, and serve others before yourself.


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Invisiblemupetmower
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Re: Can you mix spores [Re: Eywa_devotee]
    #23462987 - 07/21/16 03:35 PM (7 years, 6 months ago)

ummm why would letting the print age change anything...


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-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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InvisiblePsilocamel
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Re: Can you mix spores [Re: mupetmower]
    #23466395 - 07/22/16 03:14 PM (7 years, 6 months ago)

I think I read that bacteria dies out over time..... Not sure if that's what eywa was referring to. Not sure if that's even true.


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Offlinemrmazdarx9
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Re: Can you mix spores [Re: Psilocamel]
    #23466437 - 07/22/16 03:27 PM (7 years, 6 months ago)

Quote:

Psilocamel said:
I think I read that bacteria dies out over time..... Not sure if that's what eywa was referring to. Not sure if that's even true.



:facepalm:
Is this thread a joke?


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