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justmethebeast
BloomTheShroom
Registered: 07/07/16
Posts: 217
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inoculation, how many ccs?
#23461383 - 07/21/16 01:35 AM (7 years, 6 months ago) |
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When innoculating how many ccs do you use per quarter jar? I have 10 ccs to use and have 6 quart jars. Also will it be okay to inoculate through the polyfil?
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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I wouldn't inoculate grain jars with spores given the propensity for failure, but if I did, I would go for about 0.5cc per jar for the best chance of success.
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: inoculation, how many ccs? [Re: Inocuole]
#23461391 - 07/21/16 01:41 AM (7 years, 6 months ago) |
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Check out agar if you only have one syringe it could be wasted with putting it straight into grains then you will have to get another... agar you can make 1000 + jars with that syringe
Edited by Boogieman47 (07/21/16 01:41 AM)
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justmethebeast
BloomTheShroom
Registered: 07/07/16
Posts: 217
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Re: inoculation, how many ccs? [Re: Boogieman47]
#23461398 - 07/21/16 01:46 AM (7 years, 6 months ago) |
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Well. I'm going by starter methods here. I'd much rather have an answer to what I asked, as I understand it is pretty popular for inoculation straight to grain especially for newer individuals still gaining experience?
I very much plan to look into those methods down the line but at the moment right now it is not going to work like that for me.
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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It actually isn't popular because you can have a contaminated syringe but I'd go with what inocule said and Ya you can inoculate through the poly
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justmethebeast
BloomTheShroom
Registered: 07/07/16
Posts: 217
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Re: inoculation, how many ccs? [Re: Boogieman47]
#23461410 - 07/21/16 01:58 AM (7 years, 6 months ago) |
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So .5 ccs per quart jar?
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Do you mean a quart jar or a quarter pint?
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justmethebeast
BloomTheShroom
Registered: 07/07/16
Posts: 217
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Re: inoculation, how many ccs? [Re: Boogieman47]
#23461417 - 07/21/16 02:03 AM (7 years, 6 months ago) |
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I was meaning a quart jar. Right after PCing 6 of them I was wanting to know how many ccs each should I use
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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I figured you meant a quart but wasn't sure ... a half cc will work people use a full cc also then just wrap the left over syringe up in a paper bag or plastic and stick it in the fridge for future use if the grains are too wet it can cause the spores to not colonize keep that in mind
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justmethebeast
BloomTheShroom
Registered: 07/07/16
Posts: 217
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Re: inoculation, how many ccs? [Re: Boogieman47]
#23461434 - 07/21/16 02:09 AM (7 years, 6 months ago) |
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Thank you very much I appreciate it man!
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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No worries
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: inoculation, how many ccs? [Re: Boogieman47]
#23461485 - 07/21/16 02:40 AM (7 years, 6 months ago) |
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Less is more with spore solutions. The more solution you put inside a jar, the higher the chances that you end up with something funky in there.
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Starter
Stranger


Registered: 05/16/03
Posts: 1,148
Loc: Australia
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You're going to shoot grain jars of quart size (near 1 liter size), yes?
That's multispore - no worries. Works a treat. I would make my own prints and syringes and usually shot 4CC per jar ensuring I got a good film around the entire inside wall of the glass. I would not shake. I would only shake the grains when about 25-30% colonised.
Here's an example of EQ multispore. Grain is wbs, with the bottom casing layer being hydroponic rockwool, which was mixed into the colonised grain to increase the water content of the casings, a critical requirement for mushrooms which are 90%+ water. The top casing being coir as I don't like the idea of glass fibres in the shrooms.
















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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: inoculation, how many ccs? [Re: Starter]
#23461517 - 07/21/16 03:02 AM (7 years, 6 months ago) |
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4cc with homemade syringes?

I can make a MS grow without even throwing a single filthy spore on my grains. For your regular newbie, spore inoculations should really be done by vendor syringes until he is confident enough in his sterile tek to make clean enough prints. The less spore solution used, the better, regardless of where the syringe is from. Homemade prints should really ever be used on agar to avoid unneccessary failures and the disappointment that comes with them.
I think Noob47 here used to inoculate with vendor syringes before he learned firsthand how useful agar is.
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Ya and didn't get shit on 18 jars after a month and they were pretty thick and chunky purply but I used whole oats so I don't know if that had any affect on it failing
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: inoculation, how many ccs? [Re: Boogieman47]
#23461538 - 07/21/16 03:15 AM (7 years, 6 months ago) |
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Oats are awesome, they were definitely not the problem. Or do you mean dehulled?
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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No, hulled oats don't get me wrong I fucking love em I just didn't know if they were a cause considering they are a bit tougher then rye or wbs
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: inoculation, how many ccs? [Re: Boogieman47]
#23461554 - 07/21/16 03:28 AM (7 years, 6 months ago) |
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Hulled oats are awesome. I've been using them exclusively for a while now. I like that they are tough, makes the prep super easy. Just boil it, strain it for 2 minutes, load the grains while still wet and PC. I leave them a bit on the dry side during the boil so the extra water that is left from loading them wet will be absorbed. Makes for a really fast prep. Takes me like 40 minutes to go from dry grains in the sack to jarred grains ready for PCing.
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Ya definitely better then soaking overnight to simmering and they are cheap fucking rye for me was 20$ for ten lbs I bought wbs twice didn't care for em this hobby takes up enough time to be wasting on prepping grain
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: inoculation, how many ccs? [Re: Boogieman47]
#23461572 - 07/21/16 03:43 AM (7 years, 6 months ago) |
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WBS is pretty easy to prep too if you don't mind waiting for the soak. Some folks soak it in hot water and leave it for 24 hours, after which they jar it and PC. It's even less work than boiling oats but the downside is the long soak.
Don't overpay for any grain just because you think it is "the best". The best grain is the one that is easily available, cheap and easy for you to prep. Some people keep on overpaying for rye just for the sake of using rye.
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Trust me I'd use dirt if I could make myc colonize on it haha I am pretty cheap when it comesto buying shit unless it's an absolute must
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Starter
Stranger


Registered: 05/16/03
Posts: 1,148
Loc: Australia
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Quote:
Supalemonhaze said: 4cc with homemade syringes?

I can make a MS grow without even throwing a single filthy spore on my grains. For your regular newbie, spore inoculations should really be done by vendor syringes until he is confident enough in his sterile tek to make clean enough prints. The less spore solution used, the better, regardless of where the syringe is from. Homemade prints should really ever be used on agar to avoid unneccessary failures and the disappointment that comes with them.
I think Noob47 here used to inoculate with vendor syringes before he learned firsthand how useful agar is.
>>>For your regular newbie, spore inoculations should really be done by vendor syringes until he is confident enough in his sterile tek to make clean enough prints.
I'll have to respectfully disagree, as agar requires a HEPA = probably too much for a noobie.
I started as a total noobie with prints sent by shroomery members back in 2003 from the US. On arrival I made my own syringes and never bought a cubie strain from a vendor, ever. It's not difficult to be clean enough to start from a print or make liquid in the kitchen.
Yes, agar and a HEPA rule for G2G, isolates, and extracting usable genetics out of sketchy prints, but easy and low cost approaches do work.
GT home made spore syringe; print originally from a member in the US back in 2003.
[/url]
This was my attempt in 2003 of the PF tek. I wasn't too fond of cakes as they hold too little water so I cased, which was not to my liking either as cakes are mostly verm so the growth was small. Again, home made spore syringe.
  
An "Australian" PF cake, and a GT casing, both from a homemade spore syringes. I did the same again with Amazon and EQ.
And whoever got prints 10+ years back never complained. Keep it simple.

 
The noobies don't need to part with one cent to the vendors for cubie strains. I suggest fellahs you trade for prints, get some animal syringes to make your own spore syringes, and $kip the vendors. If you want to get advanced buy a HEPA and grow with agar, but it's not necessary for hobby cubie growing.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: inoculation, how many ccs? [Re: Starter]
#23461633 - 07/21/16 04:38 AM (7 years, 6 months ago) |
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Quote:
I'll have to respectfully disagree, as agar requires a HEPA = probably too much for a noobie.
I only read this before I realised reading further will not be even worth it, literally stopped after the first sentence. You can do agar as successfully in a $20 tote (a SAB) as one can do with a FH. It's 2016 dude.
Edited by Supalemonhaze (07/21/16 04:43 AM)
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Oh no, an old hand stumbled in here right out of '98.... OP is only listening to the answers he wants to hear, this thread is making me cringe now.
Look, spores to grain is considered bad practice. It does not "work a treat" by today's standards. Today's standards include using agar as the primary form of inoculate. No HEPAs or other crazy shit are required for these skills.
https://www.shroomery.org/forums/showflat.php/Number/22721954 http://www.shroomery.org/forums/showflat.php/Number/19140341#19140341
IF you're set on using spores to grain, which again, is a relic of the past in terms of "user friendliness" and success rate, then you should use 0.5 cc per jar, so that you reduce the risk of including any contaminants that might be in the syringe.
Using 1cc or 2cc increases your chances of failure by 2 or 4 times respectively. Using less, you also, get this... will be able to inoculate more projects at once.
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Hugh Jorgan
Mycophile



Registered: 09/21/15
Posts: 173
Last seen: 2 years, 9 months
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Re: inoculation, how many ccs? [Re: Starter]
#23461755 - 07/21/16 06:35 AM (7 years, 6 months ago) |
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Sorry Starter agar does not require a HEPA or LFH. I have been doing Agar work in a SAB for quite some time now with great results. Its more a matter of your sterile technique. Results are more consistent with FAR FAR FAR fewer contams than any ms syringe. With agar you know what you're working with before putting it to grain.
-------------------- Always go forward! Never go straight!!
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