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InvisibleBoogieman47
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Registered: 03/05/16
Posts: 9,712
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Re: inoculation, how many ccs? [Re: Supalemonhaze]
    #23461574 - 07/21/16 03:46 AM (7 years, 6 months ago)

Trust me I'd use dirt if I could make myc colonize on it haha I am pretty cheap when it comesto buying shit unless it's an absolute must


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InvisibleStarter
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Registered: 05/16/03
Posts: 1,148
Loc: Australia
Re: inoculation, how many ccs? [Re: Supalemonhaze]
    #23461585 - 07/21/16 03:55 AM (7 years, 6 months ago)

Quote:

Supalemonhaze said:
4cc with homemade syringes?

:justno:

I can make a MS grow without even throwing a single filthy spore on my grains. For your regular newbie, spore inoculations should really be done by vendor syringes until he is confident enough in his sterile tek to make clean enough prints. The less spore solution used, the better, regardless of where the syringe is from. Homemade prints should really ever be used on agar to avoid unneccessary failures and the disappointment that comes with them.

I think Noob47 here used to inoculate with vendor syringes before he learned firsthand how useful agar is.




>>>For your regular newbie, spore inoculations should really be done by vendor syringes until he is confident enough in his sterile tek to make clean enough prints.

I'll have to respectfully disagree, as agar requires a HEPA = probably too much for a noobie. 

I started as a total noobie with prints sent by shroomery members back in 2003 from the US. On arrival I made my own syringes and never bought a cubie strain from a vendor, ever. It's not difficult to be clean enough to start from a print or make liquid in the kitchen.

Yes, agar and a HEPA rule for G2G, isolates, and extracting usable genetics out of sketchy prints, but easy and low cost approaches do work.

GT home made spore syringe; print originally from a member in the US back in 2003.

[/url]

This was my attempt in 2003 of the PF tek. I wasn't too fond of cakes as they hold too little water so I cased, which was not to my liking either as cakes are mostly verm so the growth was small. Again, home made spore syringe.



An "Australian" PF cake, and a GT casing, both from a homemade spore syringes. I did the same again with Amazon and EQ.



And whoever got prints 10+ years back never complained. Keep it simple.




The noobies don't need to part with one cent to the vendors for cubie strains. I suggest fellahs you trade for prints, get some animal syringes to make your own spore syringes, and $kip the vendors. If you want to get advanced buy a HEPA and grow with agar, but it's not necessary for hobby cubie growing.


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InvisibleSupalemonhaze
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Registered: 10/02/15
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Re: inoculation, how many ccs? [Re: Starter]
    #23461633 - 07/21/16 04:38 AM (7 years, 6 months ago)

Quote:

I'll have to respectfully disagree, as agar requires a HEPA = probably too much for a noobie. 




I only read this before I realised reading further will not be even worth it, literally stopped after the first sentence. You can do agar as successfully in a $20 tote (a SAB) as one can do with a FH. It's 2016 dude.


Edited by Supalemonhaze (07/21/16 04:43 AM)


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InvisibleInocuole
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Registered: 11/21/11
Posts: 24,863
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Re: inoculation, how many ccs? [Re: Supalemonhaze]
    #23461676 - 07/21/16 05:25 AM (7 years, 6 months ago)

Oh no, an old hand stumbled in here right out of '98....  OP is only listening to the answers he wants to hear, this thread is making me cringe now.

Look, spores to grain is considered bad practice.  It does not "work a treat" by today's standards.  Today's standards include using agar as the primary form of inoculate.  No HEPAs or other crazy shit are required for these skills.

https://www.shroomery.org/forums/showflat.php/Number/22721954
http://www.shroomery.org/forums/showflat.php/Number/19140341#19140341

IF you're set on using spores to grain, which again, is a relic of the past in terms of "user friendliness" and success rate, then you should use 0.5 cc per jar, so that you reduce the risk of including any contaminants that might be in the syringe.

Using 1cc or 2cc increases your chances of failure by 2 or 4 times respectively.  Using less, you also, get this... will be able to inoculate more projects at once. :whoa:


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OfflineHugh Jorgan
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Registered: 09/21/15
Posts: 173
Last seen: 2 years, 9 months
Re: inoculation, how many ccs? [Re: Starter]
    #23461755 - 07/21/16 06:35 AM (7 years, 6 months ago)

Sorry Starter agar does not require a HEPA  or LFH. I have been doing Agar work in a SAB for quite some time now with great results. Its more a matter of your sterile technique. Results are more consistent with FAR FAR FAR fewer contams than any ms syringe. With agar you know what you're working with before putting it to grain.


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