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Invisiblejustmethebeast
BloomTheShroom
Registered: 07/07/16
Posts: 217
inoculation, how many ccs?
    #23461383 - 07/21/16 01:35 AM (7 years, 6 months ago)

When innoculating how many ccs do you use per quarter jar? I have 10 ccs to use and have 6 quart jars. Also will it be okay to inoculate through the polyfil?


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InvisibleInocuole
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Re: inoculation, how many ccs? [Re: justmethebeast]
    #23461386 - 07/21/16 01:37 AM (7 years, 6 months ago)

I wouldn't inoculate grain jars with spores given the propensity for failure, but if I did, I would go for about 0.5cc per jar for the best chance of success.


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InvisibleBoogieman47
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Re: inoculation, how many ccs? [Re: Inocuole]
    #23461391 - 07/21/16 01:41 AM (7 years, 6 months ago)

Check out agar if you only have one syringe it could be wasted with putting it straight into grains then you will have to get another... agar you can make 1000 + jars with that syringe


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Edited by Boogieman47 (07/21/16 01:41 AM)


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Invisiblejustmethebeast
BloomTheShroom
Registered: 07/07/16
Posts: 217
Re: inoculation, how many ccs? [Re: Boogieman47]
    #23461398 - 07/21/16 01:46 AM (7 years, 6 months ago)

Well. I'm going by starter methods here. I'd much rather have an answer to what I asked, as I understand it is pretty popular for inoculation straight to grain especially for newer individuals still gaining experience?

I very much plan to look into those methods down the line but at the moment right now it is not going to work like that for me.


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InvisibleBoogieman47
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Re: inoculation, how many ccs? [Re: justmethebeast]
    #23461402 - 07/21/16 01:51 AM (7 years, 6 months ago)

It actually isn't popular because you can have a contaminated syringe but I'd go with what inocule said and Ya you can inoculate through the poly


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Invisiblejustmethebeast
BloomTheShroom
Registered: 07/07/16
Posts: 217
Re: inoculation, how many ccs? [Re: Boogieman47]
    #23461410 - 07/21/16 01:58 AM (7 years, 6 months ago)

So .5 ccs per quart jar?


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InvisibleBoogieman47
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Re: inoculation, how many ccs? [Re: justmethebeast]
    #23461411 - 07/21/16 02:00 AM (7 years, 6 months ago)

Do you mean a quart jar or a quarter pint?


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Invisiblejustmethebeast
BloomTheShroom
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Re: inoculation, how many ccs? [Re: Boogieman47]
    #23461417 - 07/21/16 02:03 AM (7 years, 6 months ago)

I was meaning a quart jar. Right after PCing 6 of them I was wanting to know how many ccs each should I use


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InvisibleBoogieman47
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Re: inoculation, how many ccs? [Re: justmethebeast]
    #23461428 - 07/21/16 02:07 AM (7 years, 6 months ago)

I figured you meant a quart but wasn't sure ... a half cc will work people use a full cc also then just wrap the left over syringe up in a paper bag or plastic and stick it in the fridge for future use if the grains are too wet it can cause the spores to not colonize keep that in mind


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Invisiblejustmethebeast
BloomTheShroom
Registered: 07/07/16
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Re: inoculation, how many ccs? [Re: Boogieman47]
    #23461434 - 07/21/16 02:09 AM (7 years, 6 months ago)

Thank you very much I appreciate it man!


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InvisibleBoogieman47
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Re: inoculation, how many ccs? [Re: justmethebeast]
    #23461436 - 07/21/16 02:10 AM (7 years, 6 months ago)

No worries


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InvisibleSupalemonhaze
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Re: inoculation, how many ccs? [Re: Boogieman47]
    #23461485 - 07/21/16 02:40 AM (7 years, 6 months ago)

Less is more with spore solutions. The more solution you put inside a jar, the higher the chances that you end up with something funky in there.


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InvisibleStarter
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Re: inoculation, how many ccs? [Re: justmethebeast]
    #23461512 - 07/21/16 02:56 AM (7 years, 6 months ago)

You're going to shoot grain jars of quart size (near 1 liter size), yes?

That's multispore - no worries. Works a treat. I would make my own prints and syringes and usually shot 4CC per jar ensuring I got a good film around the entire inside wall of the glass. I would not shake. I would only shake the grains when about 25-30% colonised.

Here's an example of EQ multispore. Grain is wbs, with the bottom casing layer being hydroponic rockwool, which was mixed into the colonised grain to increase the water content of the casings, a critical requirement for mushrooms which are 90%+ water. The top casing being coir as I don't like the idea of glass fibres in the shrooms.



















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Convert Metric and Imperial.


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InvisibleSupalemonhaze
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Re: inoculation, how many ccs? [Re: Starter]
    #23461517 - 07/21/16 03:02 AM (7 years, 6 months ago)

4cc with homemade syringes?

:justno:

I can make a MS grow without even throwing a single filthy spore on my grains. For your regular newbie, spore inoculations should really be done by vendor syringes until he is confident enough in his sterile tek to make clean enough prints. The less spore solution used, the better, regardless of where the syringe is from. Homemade prints should really ever be used on agar to avoid unneccessary failures and the disappointment that comes with them.

I think Noob47 here used to inoculate with vendor syringes before he learned firsthand how useful agar is.


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InvisibleBoogieman47
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Re: inoculation, how many ccs? [Re: Supalemonhaze]
    #23461531 - 07/21/16 03:13 AM (7 years, 6 months ago)

Ya and didn't get shit on 18 jars after a month and they were pretty thick and chunky purply but I used whole oats so I don't know if that had any affect on it failing


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InvisibleSupalemonhaze
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Re: inoculation, how many ccs? [Re: Boogieman47]
    #23461538 - 07/21/16 03:15 AM (7 years, 6 months ago)

Oats are awesome, they were definitely not the problem. Or do you mean dehulled?


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InvisibleBoogieman47
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Re: inoculation, how many ccs? [Re: Supalemonhaze]
    #23461541 - 07/21/16 03:18 AM (7 years, 6 months ago)

No, hulled oats don't get me wrong I fucking love em I just didn't know if they were a cause considering they are a bit tougher then rye or wbs


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InvisibleSupalemonhaze
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Re: inoculation, how many ccs? [Re: Boogieman47]
    #23461554 - 07/21/16 03:28 AM (7 years, 6 months ago)

Hulled oats are awesome. I've been using them exclusively for a while now. I like that they are tough, makes the prep super easy. Just boil it, strain it for 2 minutes, load the grains while still wet and PC. I leave them a bit on the dry side during the boil so the extra water that is left from loading them wet will be absorbed. Makes for a really fast prep. Takes me like 40 minutes to go from dry grains in the sack to jarred grains ready for PCing.


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InvisibleBoogieman47
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Re: inoculation, how many ccs? [Re: Supalemonhaze]
    #23461559 - 07/21/16 03:32 AM (7 years, 6 months ago)

Ya definitely better then soaking overnight to simmering and they are cheap fucking rye for me was 20$ for ten lbs I bought wbs twice didn't care for em this hobby takes up enough time to be wasting on prepping grain


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InvisibleSupalemonhaze
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Re: inoculation, how many ccs? [Re: Boogieman47]
    #23461572 - 07/21/16 03:43 AM (7 years, 6 months ago)

WBS is pretty easy to prep too if you don't mind waiting for the soak. Some folks soak it in hot water and leave it for 24 hours, after which they jar it and PC. It's even less work than boiling oats but the downside is the long soak.

Don't overpay for any grain just because you think it is "the best". The best grain is the one that is easily available, cheap and easy for you to prep. Some people keep on overpaying for rye just for the sake of using rye.


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