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InvisibleInocuole
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Re: Strain Isolations on Agar, Pics and Questions [Re: c10h12n2o]
    #23573439 - 08/24/16 04:21 PM (7 years, 5 months ago)

Quote:

weetsie said:
Quote:

Supalemonhaze said:
Quote:

weetsie said:
Even when you don't dilute spores and start with a big black dot  your first plate you can still get isolates in less than 5 transfers.





I'd like to see you try and pull this off and document it.




I'll take you up on that.

Watch this space.





:standingby:


--------------------
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Offlinespacechildo
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Re: Strain Isolations on Agar, Pics and Questions [Re: c10h12n2o]
    #23573461 - 08/24/16 04:27 PM (7 years, 5 months ago)

Quote:

c10h12n2o said:
1) its perfectly symmetrical to the agar i transferred though, and using calipers to measure from the edge of the transferred agar to the leading edge of the myc it is almost dead even all the way around. this plate is either 11 or 12 transfers in

2) btw i had been meaning to ask you something spacechildo: when you have a plate that is heavily sectored showing 2 distinct kinds of myc, and there are 3-4 sections of aggressive rhizo myc and 3-4 linear sectors, and some or all of the rhizo sectors look similar, or are even connected, do you transfer all the good looking sectors, even though there is a good chance that some of them are the same? especially late into a series of transfers, like 10-15th plate.

3) sometimes lately it has seemed like i am transferring the same thing twice on the same plate, what appears to be 4 sectors might only be 1 "sector" that is on top/under another sector that is diving it (not to say that this single "interrupted sector" is an individual strain, of course there could be numerous compatible strains forming the same "sector")

4) i suppose there is no harm in labeling and testing them all separately, but im working on so much right now that i would like to minimize wasted time/space on testing strains X, Y, and Z against each other only to discover/suspect that they are indeed only one strain.

let me know if i need to clarify anything. i would really like to hear your thoughts on this and potential ways to negate this possibility without missing out on potentially valuable strains

warm regards




1, well try just 1 more transfer and make it real small, I still doubt it but I've been wrong before :shrug:

2, just look for fast growing strong looking myc. all your plates seem nice and healthy so just pick the fastest growing sectors as that's the only trait you can see on agar.

3, that's confusing :lol: its perfectly normal that sectors grow together and what looks like 1 is really 10 which you'll find out later on as you keep transferring.

4, yeah that's a bitch, in a perfect world you'd test them all but :shrug: do the amount of searching you can/have time for.


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OfflineRooster Cogburn
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Re: Strain Isolations on Agar, Pics and Questions [Re: PsilocyBen17]
    #23573528 - 08/24/16 04:45 PM (7 years, 5 months ago)

Quote:

PsilocyBen17 said:
Tonight I'm making slants for the first time. I don't have parafilm as I've never used it....would micropore tape be a fine substitute for taping around the caps?



Once I PC these I'll put them on the bench at a slight angle and drop clean wedges in once they solidify. I wish they had a wider mouthed, its going to be a pain getting wedges out when I need them again,  but I'm working with what I've got.

I plan on keeping them stored in a ziplock inside of jar with SFD filter inside the fridge.

Tips?




Those are plenty wide, you want to put a tiny piece of mycelium in there.
My biggest tip that makes it simple is getting your sample with your right hand and unscrewing the top between your pinky and ring finger of the transfer hand and doing the transfer while holding the cap like that then it's easy to screw back on.
The most valuable tool I think for making slants is a test tube holder, simply fill the tubes in the holder, screw caps on tight and throw the whole thing in the PC, pull it out and lay the rack at an angle and wait to cool, then it's really nice for doing the actual work.
The only thing I think about the micropore tape is it would dry the culture out faster over time, but better than nothing I guess, you do need GE in those things. Im not sure a jar is necessary for storing, is there a specific reason for doing this?


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InvisibleMunchauzen
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Re: Strain Isolations on Agar, Pics and Questions [Re: spacechildo]
    #23573768 - 08/24/16 06:19 PM (7 years, 5 months ago)

Quote:

spacechildo said:
If I had a dollar for every time a noob said he'd prove something wrong and even went as far as updating his thread I'd still be a poor son of a bitch!



If I had a dime for every time a noob said he'd prove something wrong and even went as far as updating his thread I'd have ZERO DIMES



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OfflineTedTheHighlighter
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Re: Strain Isolations on Agar, Pics and Questions [Re: Munchauzen]
    #23573787 - 08/24/16 06:26 PM (7 years, 5 months ago)

Quote:

Munchauzen said:
Quote:

spacechildo said:
If I had a dollar for every time a noob said he'd prove something wrong and even went as far as updating his thread I'd still be a poor son of a bitch!



If I had a dime for every time a noob said he'd prove something wrong and even went as far as updating his thread I'd have ZERO DIMES






Well, weetsie has begun his experiment and already updated the thread soooo i guess someone owes you ten cents
Props to weetsie for actually carrying out the experiment


--------------------
Alice asked the Cheshire Cat, who was sitting in a tree, “What road do I take?”

The cat asked, “Where do you want to go?”

“I don’t know,” Alice answered.

“Then,” said the cat, “it really doesn’t matter, does it?”


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InvisibleMunchauzen
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Re: Strain Isolations on Agar, Pics and Questions [Re: TedTheHighlighter]
    #23573803 - 08/24/16 06:31 PM (7 years, 5 months ago)

Quote:

TedTheHighlighter said:
Quote:

Munchauzen said:
Quote:

spacechildo said:
If I had a dollar for every time a noob said he'd prove something wrong and even went as far as updating his thread I'd still be a poor son of a bitch!



If I had a dime for every time a noob said he'd prove something wrong and even went as far as updating his thread I'd have ZERO DIMES






Well, weetsie has begun his experiment and already updated the thread soooo i guess someone owes you ten cents
Props to weetsie for actually carrying out the experiment



he hasn't updated shit. a random picture of an empty plate from over a month ago is not an update, its a waste of a post and our time. so, I'm sticking with NO DIMES.


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Offlinespacechildo
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Re: Strain Isolations on Agar, Pics and Questions [Re: Munchauzen]
    #23573809 - 08/24/16 06:33 PM (7 years, 5 months ago)

Quote:

Munchauzen said:
Quote:

spacechildo said:
If I had a dollar for every time a noob said he'd prove something wrong and even went as far as updating his thread I'd still be a poor son of a bitch!



If I had a dime for every time a noob said he'd prove something wrong and even went as far as updating his thread I'd have ZERO DIMES






:philososloth:

havent seen any updates myself :shrug: should get its own thread for sure if its happening!


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InvisibleMunchauzen
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Re: Strain Isolations on Agar, Pics and Questions [Re: spacechildo]
    #23573813 - 08/24/16 06:35 PM (7 years, 5 months ago)

this is what dude considered an 'update'

Quote:

weetsie said:
Scraped up at least half a square inch of spores and dumped them in a pile on a plate.



Feeling confident :dancing:





https://www.shroomery.org/forums/showflat.php/Number/23468297#23468297

I could have made 4 transfers and finished this shit within a month. No need to let the plates grow out. You could probably get to the 4th transfer in 2 weeks, if you actually tried.


Edited by Munchauzen (08/24/16 06:35 PM)


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OfflineTedTheHighlighter
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Re: Strain Isolations on Agar, Pics and Questions [Re: weetsie]
    #23573826 - 08/24/16 06:39 PM (7 years, 5 months ago)

Quote:

weetsie said:


Took 2 transfers from the first plate today. :dancer:




Is this not an update?


--------------------
Alice asked the Cheshire Cat, who was sitting in a tree, “What road do I take?”

The cat asked, “Where do you want to go?”

“I don’t know,” Alice answered.

“Then,” said the cat, “it really doesn’t matter, does it?”


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InvisibleMunchauzen
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Re: Strain Isolations on Agar, Pics and Questions [Re: weetsie]
    #23573890 - 08/24/16 07:02 PM (7 years, 5 months ago)

Quote:

weetsie said:


Took 2 transfers from the first plate today. :dancer:



:lol: I blame space for priming me with bad info

still, dont know why he is waiting for it to grow out to the edge of the plate to make a transfer. Weetsie, stop pussy-footin' around and get crackin'!


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Offlinespacechildo
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Re: Strain Isolations on Agar, Pics and Questions [Re: Munchauzen]
    #23573914 - 08/24/16 07:09 PM (7 years, 5 months ago)

IMO its a terrible excuse for an update. horrible pics and no telling whats going on. but yeah seems I got ma dollah as well!


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OfflinePsilocyBen17
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Re: Strain Isolations on Agar, Pics and Questions [Re: Rooster Cogburn]
    #23574225 - 08/24/16 09:06 PM (7 years, 5 months ago)

Quote:

Rooster Cogburn said:
Quote:

PsilocyBen17 said:
Tonight I'm making slants for the first time. I don't have parafilm as I've never used it....would micropore tape be a fine substitute for taping around the caps?



Once I PC these I'll put them on the bench at a slight angle and drop clean wedges in once they solidify. I wish they had a wider mouthed, its going to be a pain getting wedges out when I need them again,  but I'm working with what I've got.

I plan on keeping them stored in a ziplock inside of jar with SFD filter inside the fridge.

Tips?




Those are plenty wide, you want to put a tiny piece of mycelium in there.
My biggest tip that makes it simple is getting your sample with your right hand and unscrewing the top between your pinky and ring finger of the transfer hand and doing the transfer while holding the cap like that then it's easy to screw back on.
The most valuable tool I think for making slants is a test tube holder, simply fill the tubes in the holder, screw caps on tight and throw the whole thing in the PC, pull it out and lay the rack at an angle and wait to cool, then it's really nice for doing the actual work.
The only thing I think about the micropore tape is it would dry the culture out faster over time, but better than nothing I guess, you do need GE in those things. Im not sure a jar is necessary for storing, is there a specific reason for doing this?




Cool, thanks kindly for your response...

No reason for the jar really, just a convient way to store them. They all fit in there nicely, why not throw an sfd on there just cuz...

I DID forget to prehydrate the popsicle sticks :facepalm3:. Hopefully they don't dry out super fast cuz of this....worst case senario I can transfer to new slants 6 months down the road.


--------------------
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Invisibleweetsie
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Re: Strain Isolations on Agar, Pics and Questions [Re: spacechildo]
    #23574881 - 08/25/16 02:06 AM (7 years, 5 months ago)

Quote:

spacechildo said:
IMO its a terrible excuse for an update. horrible pics and no telling whats going on. but yeah seems I got ma dollah as well!




No telling what's going on?

I transferred two small pieces of mycelium from the leading edge of the plate on the left to the two on the right. But that much was obvious, what else is there to say. :lol:

I'll make the 2nd transfer in a couple of hours and post some photos with the lids off.


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Offlinespacechildo
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Re: Strain Isolations on Agar, Pics and Questions [Re: weetsie]
    #23575096 - 08/25/16 06:07 AM (7 years, 5 months ago)

your initial plate looks like shit but I really cant zoom in on it.
and just for the sake of argument, lets say you did get an isolate in 5 transfers. are you gonna use this thread to show it can be done?
"hey dudes, just look for my posts somewhere in between c12's posts" :confused2:


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Invisibleblindingleaf
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Re: Strain Isolations on Agar, Pics and Questions [Re: spacechildo] * 1
    #23575109 - 08/25/16 06:17 AM (7 years, 5 months ago)

i got an MS isolate in 8 transfers (so the ninth plate if u include the initial germination plate) only one time.



pure luck


--------------------
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Invisiblemary fairchild
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Re: Strain Isolations on Agar, Pics and Questions [Re: blindingleaf]
    #23575245 - 08/25/16 07:57 AM (7 years, 5 months ago)

Quote:

blindingleaf said:
i got an MS isolate in 8 transfers (so the ninth plate if u include the initial germination plate) only one time.



pure luck




Is that isolate a Pan cambo? Looks like thats what you wrote on the plate. If so, its an interesting growth form for a Pan.
The reason I ask is I'm working on a cambo Sandos isolate right now.
Have you had success taking Pan isolates to fruiting?


Edited by mary fairchild (08/26/16 06:18 AM)


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InvisibleMad Season
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Re: Strain Isolations on Agar, Pics and Questions [Re: mary fairchild]
    #23575380 - 08/25/16 09:03 AM (7 years, 5 months ago)

No, that's not panaeolus mycelium.

This is



--------------------
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Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

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Invisiblemary fairchild
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Re: Strain Isolations on Agar, Pics and Questions [Re: Mad Season]
    #23575527 - 08/25/16 09:55 AM (7 years, 5 months ago)

It looks kinda more "fibrous" than most Pans- but my cambo Sandos looks that way too.



Edited by mary fairchild (08/25/16 09:56 AM)


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Offlineguyute22
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Re: Strain Isolations on Agar, Pics and Questions [Re: mary fairchild]
    #23575561 - 08/25/16 10:03 AM (7 years, 5 months ago)

:creepylurker:


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OfflineRooster Cogburn
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Re: Strain Isolations on Agar, Pics and Questions [Re: weetsie]
    #23575651 - 08/25/16 10:34 AM (7 years, 5 months ago)

Quote:

weetsie said:
Quote:

spacechildo said:
IMO its a terrible excuse for an update. horrible pics and no telling whats going on. but yeah seems I got ma dollah as well!




No telling what's going on?

I transferred two small pieces of mycelium from the leading edge of the plate on the left to the two on the right. But that much was obvious, what else is there to say. :lol:

I'll make the 2nd transfer in a couple of hours and post some photos with the lids off.




Wheetsie, your going to set off a shit storm if you get this! It's definitely gonna need it's own thread man.


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