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OfflineKenetic
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Re: Strain Isolations on Agar, Pics and Questions [Re: c10h12n2o]
    #23563587 - 08/21/16 05:33 PM (7 years, 5 months ago)

Where'd you get the zip bags for your petri's?  That's a good idea.  Tedious with all the plates I'm getting into but it would make them easily examinable.  I have 100 mm dishes now but I ordered 100 60 mm dishes, 10 per sleeve and I can easily fit them in my glovebox.


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Invisiblec10h12n2o
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Re: Strain Isolations on Agar, Pics and Questions [Re: Kenetic]
    #23563616 - 08/21/16 05:44 PM (7 years, 5 months ago)

certainly makes for a fun species to experiment with, my mote toting friend :smile: and an inspiring thought :smile:

Quote:

kenetic said:
Where'd you get the zip bags for your petri's?  That's a good idea.  Tedious with all the plates I'm getting into but it would make them easily examinable.  I have 100 mm dishes now but I ordered 100 60 mm dishes, 10 per sleeve and I can easily fit them in my glovebox.




I had them left over from another project, i got them from Uline (best prices on any kind of bag or containers) and since some perfectly fit the petris i put them to use :smile:

definitely got tedious though. i eventually got a big roll of parafilm from my lab supply place and have been loving it! i got the 4" kind and cut a 1" strip that easily fits around the edge, works beautifully, like they were made for each other :smile: i still follow up by putting them in sandwich bags just for an extra layer of protection while i ogle them

nice! i love those little petris, but just dont forget one in the incubator, itll be solid white in no time lol

i actually just finished building a big stealth SAB so that i could do g2g and 3 sleeves (60) plates at a time :smile: working great so far!! here is a link


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OfflineTedTheHighlighter
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Re: Strain Isolations on Agar, Pics and Questions [Re: bodhisatta]
    #23571003 - 08/23/16 10:22 PM (7 years, 5 months ago)

Quote:

Trusted Cultivator said:
Staments is a great researcher, great grower, but a miserable scientist. He touts mushrooms as a legitimate cure for cancer, and has never cured a single person




I have heard him say in one of his seminars that his mother's cancer was cured with the help of mushroom capsules. Now, that's just what he said, but I don't think I would think he would lie about something like that. I have a lot of respect for Stamets and the great ways he uses mycology to help the world.
I wouldn't really doubt that mushrooms helped cure his mother and I wouldn't doubt that they could help others. I've heard from RR about the powerful anti-cancer properties of certain mushrooms and believe that, well idk, it could be a thing.


--------------------
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Invisiblec10h12n2o
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Re: Strain Isolations on Agar, Pics and Questions [Re: TedTheHighlighter]
    #23572436 - 08/24/16 10:31 AM (7 years, 5 months ago)

Quote:

TedTheHighlighter said:
Quote:

Trusted Cultivator said:
Staments is a great researcher, great grower, but a miserable scientist. He touts mushrooms as a legitimate cure for cancer, and has never cured a single person




I have heard him say in one of his seminars that his mother's cancer was cured with the help of mushroom capsules. Now, that's just what he said, but I don't think I would think he would lie about something like that. I have a lot of respect for Stamets and the great ways he uses mycology to help the world.
I wouldn't really doubt that mushrooms helped cure his mother and I wouldn't doubt that they could help others. I've heard from RR about the powerful anti-cancer properties of certain mushrooms and believe that, well idk, it could be a thing.




yeah, i COMPLETELY disagree with bodhi on that account. I think nothing short of arrogance and naivety could lead someone who probably doesnt have a single academic paper published to look down on one of the hardest working, most innovative researchers in the field of mycology, with dozens of studies to his name

he absolutely does not "tout mushrooms as a legitimate cure for cancer" , he researches the potential mycological compounds might have in treating various types of cancer. And he has good reason for doing so, there has been some really amazing research lately. the fact that someone is smart enough to recognize the potential for these compounds is flat out incompatible with an oversimplification like that. stamets is brilliant, humble, and dedicated and we are lucky to have him

a few of his studies to put this nonsense in context:
We're sitting on a mould mine.
Stamets, Paul
Source:
New Scientist. 2/13/2016, Vol. 229 Issue 3060, p28-29. 2p. 3 Color Photographs.

Truly Magical MUSHROOMS.
Authors:
Baker, Linda
Source:
Utne. Mar/Apr2003, Issue 116, p78. 5p. 3 Color Photographs.

MUSHROOM MANIFESTO. (cover story)
Authors:
MILLER, KENNETH
Source:
Discover. Jul/Aug2013, Vol. 34 Issue 6, p38-47. 10p.

Mushrooms Proposed for Gulf Spill Cleanup.
Source:
Oil Spill Intelligence Report. 11/11/2010, Vol. 33 Issue 47, p4-4. 1/3p.

Mushrooms: Ancient Allies for Modern Medicine.
Authors:
Stamets, Paul
Source:
Share Guide. Nov/Dec2010, Issue 112, p20-29. 3p.

Earth's Natural Internet.
Authors:
Stamets, Paul
Source:
Whole Earth. Fall99, Issue 98, p74. 4p. 5 Black and White Photographs.

Novel Antimicrobials from mushrooms.
Authors:
Stamets, Paul
Source:
HerbalGram. Spring2002, Issue 54, p28-33. 6p.

Crude Extracts of Marine-derived and Soil Fungi of the Genus Neosartorya Exhibit Selective Anticancer Activity by Inducing Cell Death in Colon, Breast and Skin Cancer Cell Lines.
Authors:
Ramos, Alice Abreu1
Castro-Carvalho, Bruno1,2
Prata-Sena, Maria1,2
Dethoup, Tida3
Buttachon, Suradet1,4
Kijjoa, Anake1,4
Rocha, Eduardo1,2 erocha@icbas.up.pt
Source:
Pharmacognosy Research. Jan-Mar2016, Vol. 8 Issue 1, p8-15. 8p.

Potential of four marine-derived fungi extracts as anti-proliferative and cell death-inducing agents in seven human cancer cell lines.
Authors:
Ramos AA; Interdisciplinary Center for Marine and Environmental Research (CIIMAR), CIMAR Associate Laboratory (CIMAR LA), University of Porto (U. Porto), Rua dos Bragas, nº 289, 4050-123 Porto, Portugal.
Prata-Sena M; Interdisciplinary Center for Marine and Environmental Research (CIIMAR), CIMAR Associate Laboratory (CIMAR LA), University of Porto (U. Porto), Rua dos Bragas, nº 289, 4050-123 Porto, Portugal; ICBAS - Institute of Biomedical Sciences Abel Salazar, University of Porto (U. Porto), Rua de Jorge Viterbo Ferreira, nº 228, 4050-313 Porto, Portugal.
Castro-Carvalho B; Interdisciplinary Center for Marine and Environmental Research (CIIMAR), CIMAR Associate Laboratory (CIMAR LA), University of Porto (U. Porto), Rua dos Bragas, nº 289, 4050-123 Porto, Portugal; ICBAS - Institute of Biomedical Sciences Abel Salazar, University of Porto (U. Porto), Rua de Jorge Viterbo Ferreira, nº 228, 4050-313 Porto, Portugal.
Dethoup T; Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Bangkok, Thailand.
Buttachon S; Interdisciplinary Center for Marine and Environmental Research (CIIMAR), CIMAR Associate Laboratory (CIMAR LA), University of Porto (U. Porto), Rua dos Bragas, nº 289, 4050-123 Porto, Portugal; ICBAS - Institute of Biomedical Sciences Abel Salazar, University of Porto (U. Porto), Rua de Jorge Viterbo Ferreira, nº 228, 4050-313 Porto, Portugal.
Kijjoa A; Interdisciplinary Center for Marine and Environmental Research (CIIMAR), CIMAR Associate Laboratory (CIMAR LA), University of Porto (U. Porto), Rua dos Bragas, nº 289, 4050-123 Porto, Portugal; ICBAS - Institute of Biomedical Sciences Abel Salazar, University of Porto (U. Porto), Rua de Jorge Viterbo Ferreira, nº 228, 4050-313 Porto, Portugal.
Rocha E; Interdisciplinary Center for Marine and Environmental Research (CIIMAR), CIMAR Associate Laboratory (CIMAR LA), University of Porto (U. Porto), Rua dos Bragas, nº 289, 4050-123 Porto, Portugal; ICBAS - Institute of Biomedical Sciences Abel Salazar, University of Porto (U. Porto), Rua de Jorge Viterbo Ferreira, nº 228, 4050-313 Porto, Portugal. Electronic address: erocha@icbas.up.pt.
Source:
Asian Pacific Journal Of Tropical Medicine [Asian Pac J Trop Med] 2015 Oct; Vol. 8 (10), pp. 798-806. Date of Electronic Publication: 2015 Sep 25.

Sloth hair as a novel source of fungi with potent anti-parasitic, anti-cancer and anti-bacterial bioactivity.
Authors:
Higginbotham S; Smithsonian Tropical Research Institute, Panama, Republic of Panama.
Wong WR; Department of Chemistry and Biochemistry, University of California Santa Cruz, Santa Cruz, California, United States of America.
Linington RG; Department of Chemistry and Biochemistry, University of California Santa Cruz, Santa Cruz, California, United States of America.
Spadafora C; Instituto de Investigaciones Científicas y Servicios de Alta Tecnología, Panama, Republic of Panama.
Iturrado L; Smithsonian Tropical Research Institute, Panama, Republic of Panama.
Arnold AE; School of Plant Sciences, University of Arizona, Tucson, Arizona, United States of America.
Source:
Plos One [PLoS One] 2014 Jan 15; Vol. 9 (1), pp. e84549. Date of Electronic Publication: 20140115 (Print Publication: 2014).

Aurovertin-Type Polyketides from Calcarisporium arbuscula with Potent Cytotoxic Activities against Triple-Negative Breast Cancer.
Authors:
Zhao, Hong1
Wu, Rui2
Ma, Lie-Feng2
Wo, Li-Ke1
Hu, Yuan-Yuan1
Chen, Chao1
Zhan, Zha-Jun2,3
Source:
Helvetica Chimica Acta. Jul2016, Vol. 99 Issue 7, p543-546. 4p.

Taxol produced from endophytic fungi induces apoptosis in human breast, cervical and ovarian cancer cells.
Authors:
Wang X; Key Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin, China E-mail : zybin395@126.com.
Wang C
Sun YT
Sun CZ
Zhang Y
Wang XH
Zhao K
Source:
Asian Pacific Journal Of Cancer Prevention: APJCP [Asian Pac J Cancer Prev] 2015; Vol. 16 (1), pp. 125-31.

Cytotoxicity Induced by Extracts of Pisolithus tinctorius Spores on Human Cancer and Normal Cell Lines—Evaluation of the Anticancer Potential.
Authors:
Alves, Ricardo1
Preto, Marco2
Vasconcelos, Vitor2,3
Oliveira, Rui S.1,4,5
Martins, Rosário1,2
Source:
Journal of Toxicology & Environmental Health: Part A. 2015, Vol. 78 Issue 13/14, p840-847. 8p.

Toward a Cancer Drug of Fungal Origin.
Authors:
Kornienko A; Department of Chemistry and Biochemistry, Texas State University, San Marcos, TX, 78666.
Evidente A; Dipartimento di Scienze Chimiche, Università di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126, Napoli, Italy.
Vurro M; Institute of Sciences of Food Production, National Research Council, Via Amendola 122/0, 70126, Bari, Italy.
Mathieu V; Laboratorie de Cancérologie et de Toxicologie Expérimentale, Faculté de Pharmacie, Université Libre de Bruxelles (ULB), Brussels, Belgium.
Cimmino A; Dipartimento di Scienze Chimiche, Università di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126, Napoli, Italy.
Evidente M; Dipartimento di Scienze Chimiche, Università di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126, Napoli, Italy.
van Otterlo WA; Department of Chemistry and Polymer Science, University of Stellenbosch, Private Bag X1, Matieland, 7602, South Africa.
Dasari R; Department of Chemistry and Biochemistry, Texas State University, San Marcos, TX, 78666.
Lefranc F; Service de Neurochirurgie, Hôpital Erasme, Université Libre de Bruxelles (ULB), Brussels, Belgium.
Kiss R; Laboratorie de Cancérologie et de Toxicologie Expérimentale, Faculté de Pharmacie, Université Libre de Bruxelles (ULB), Brussels, Belgium.
Source:
Medicinal Research Reviews [Med Res Rev] 2015 Sep; Vol. 35 (5), pp. 937-67. Date of Electronic Publication: 2015 Apr 08.

Destruxin B Isolated from Entomopathogenic Fungus Metarhizium anisopliae Induces Apoptosis via a Bcl-2 Family-Dependent Mitochondrial Pathway in Human Nonsmall Cell Lung Cancer Cells.
Authors:
Chun-Chi Wu
Tzu-Hsiu Chen
Bing-Lan Liu
Li-Chen Wu
Yung-Ching Chen
Yew-Min Tzeng
Shih-Lan Hsu
Source:
Evidence-based Complementary & Alternative Medicine (eCAM). 2013, Vol. 2013, p1-11. 11p. 2 Diagrams, 4 Graphs.

The Marine-Derived Fungus Clonostachys rosea, Source of a Rare Conjugated 4-Me-6E,8E-hexadecadienoic Acid Reducing Viability of MCF-7 Breast Cancer Cells and Gene Expression of Lipogenic Enzymes.
Authors:
Dias AC; Faculty of Pharmacy, University of Nantes, MMS; 9, Rue Bias, 44000 Nantes, France. acamiladias@gmail.com.
Ruiz N; Faculty of Pharmacy, University of Nantes, MMS; 9, Rue Bias, 44000 Nantes, France. nicolas.ruiz@univ-nantes.fr.
Couzinet-Mossion A; Faculty of Pharmacy, University of Nantes, MMS; 9, Rue Bias, 44000 Nantes, France.
Bertrand S; Faculty of Pharmacy, University of Nantes, MMS; 9, Rue Bias, 44000 Nantes, France. samuel.bertrand@univ-nantes.fr.
Duflos M; Faculty of Pharmacy, University of Nantes, IICiMed, 9 Rue Bias, 44000 Nantes, France. muriel.duflos@univ-nantes.fr.
Pouchus YF; Faculty of Pharmacy, University of Nantes, MMS; 9, Rue Bias, 44000 Nantes, France. yves-francois.pouchus@univ-nantes.fr.
Barnathan G; Faculty of Pharmacy, University of Nantes, MMS; 9, Rue Bias, 44000 Nantes, France. gilles.barnathan@univ-nantes.fr.
Nazih H; Faculty of Pharmacy, University of Nantes, MMS; 9, Rue Bias, 44000 Nantes, France. el-hassane.nazih@univ-nantes.fr.
Wielgosz-Collin G; Faculty of Pharmacy, University of Nantes, MMS; 9, Rue Bias, 44000 Nantes, France. gaetane.wielgosz-collin@univ-nantes.fr.
Source:
Marine Drugs [Mar Drugs] 2015 Aug 06; Vol. 13 (8), pp. 4934-48. Date of Electronic Publication: 2015 Aug 06.

I could go on for quite a while, i have literally hundreds of these bookmarked, and would suggest anyone who seeks to form an opinion on the validity of anti-cancer fungal research (or stamets brilliance for getting so many started researching in that direction) should be required to actually read some of the literature.

CONTEXT CONTEXT CONTEXT, anyone who actually keeps up with the research on this stuff knows that stamets contribution is massive, and would be grateful that he is so committed to learning more about these things


--------------------

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"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
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Offlinespacechildo
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Re: Strain Isolations on Agar, Pics and Questions [Re: c10h12n2o]
    #23572537 - 08/24/16 11:07 AM (7 years, 5 months ago)

those that know stamets well understands his points but have a problem with the way he uses his position to earn $$ instead of focusing on the works.
stamets is also well known to exaggerate his truths and statements to an extent where he can't back his claims up. that doesnt take away all his done for the community tho, but that's not what's being said either.


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Invisiblec10h12n2o
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Re: Strain Isolations on Agar, Pics and Questions [Re: spacechildo]
    #23572651 - 08/24/16 12:06 PM (7 years, 5 months ago)

i think someone would need to contribute a WHOLE LOT to the subject of mycology and medicine before they could look down on stamets, in any respect

i get what you are saying, he certainly has the propensity to get excited about his work, and he is definitely guilty of tryng to dumb things down enough in public speaking events that the DoD guy asking "so does each spore turn into a mushroom?" and the people who would lose interest if he said the word "cytotoxic" can get the gist of what he is saying.

his big thing lately has been saying that the old growth forest should be saved as a matter of national defense, which is certainly a radical idea that many a defense contractor would find laughable. but if you look at the work he has been doing, and more importantly, the studies that have been citing him and advancing this line of research, it is easy to see how he could get excited

it bugs me when someone has to take something out of context to criticize it, its just not valid


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


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Offlinespacechildo
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Re: Strain Isolations on Agar, Pics and Questions [Re: c10h12n2o]
    #23572854 - 08/24/16 01:35 PM (7 years, 5 months ago)

saving old forest and peat moss ground isn't a "new thing" its been known for "ever" but the industry just doesnt give a flying fuck about the environment.


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OfflineKenetic
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Re: Strain Isolations on Agar, Pics and Questions [Re: spacechildo]
    #23572948 - 08/24/16 02:06 PM (7 years, 5 months ago)

The environment doesn't actually exist.


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



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Re: Strain Isolations on Agar, Pics and Questions [Re: Kenetic]
    #23572968 - 08/24/16 02:14 PM (7 years, 5 months ago)

muda earth then..


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OfflineRooster Cogburn
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Re: Strain Isolations on Agar, Pics and Questions [Re: spacechildo]
    #23573007 - 08/24/16 02:27 PM (7 years, 5 months ago)

Uhhhh, gaia?


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OfflineRooster Cogburn
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Re: Strain Isolations on Agar, Pics and Questions [Re: Rooster Cogburn]
    #23573073 - 08/24/16 02:46 PM (7 years, 5 months ago)

I have a lot of respect for Stamets due to the fact I learned nearly everything about cultivating oysters from GGMM and nothing else, but I feel the main reason people LOVE hating him is because his company is ridiculous!Fungi Perfecti is a company that takes advantage of new growers, not only that but their cultures really suck, they are on the bottom of the chain for commercial cultures which is the ONE thing they should have right.
There was that thread recently where that old timer Parecelusgold said Stamets was an asshole. I got a kick out of that, says nothing about his skill set and research, but he does have that feel to him.
One last thing, people always say, "Stamets doesn't grow cubes", this right here, considering he had research grants and legally grew them and documented them, I can guarantee he grew more cubes than we will ever see between his researching and his college days. Asshole, horrible business owner, turkey tail cancer curer or not, he is a good cultivator and writer.


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Invisiblec10h12n2o
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Re: Strain Isolations on Agar, Pics and Questions [Re: Rooster Cogburn]
    #23573164 - 08/24/16 03:08 PM (7 years, 5 months ago)

lol i bet he can come off that way :smile: most professor-types can

ive never ordered spawn or anything like that from them, they definitely have decent prices on hepa filters though (and crazy prices on assembled hoods lol), but if their commercial cultures are anything short of amazing that would certainly be a let-down, like you said, thats one thing they should have right. Very lame if they are using his name to sell shitty cultures

FP is a big company, i was amazed to see how many employees are pictured in their current catalog


I doubt he has a whole lot to do with running it these days, probably spends most of his time on research projects, many of which seem to be classified these days

right there with you codburn, mad respect

UPDATE: here are a couple of the more rhizo plates i mentioned. one has grown out, and the other 2 are more current. one seems to be an isolate :smile:


--------------------

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"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
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Re: Strain Isolations on Agar, Pics and Questions [Re: Rooster Cogburn]
    #23573171 - 08/24/16 03:09 PM (7 years, 5 months ago)

Quote:

Rooster Cogburn said:
I have a lot of respect for Stamets due to the fact I learned nearly everything about cultivating oysters from GGMM and nothing else, but I feel the main reason people LOVE hating him is because his company is ridiculous!Fungi Perfecti is a company that takes advantage of new growers, not only that but their cultures really suck, they are on the bottom of the chain for commercial cultures which is the ONE thing they should have right.
There was that thread recently where that old timer Parecelusgold said Stamets was an asshole. I got a kick out of that, says nothing about his skill set and research, but he does have that feel to him.
One last thing, people always say, "Stamets doesn't grow cubes", this right here, considering he had research grants and legally grew them and documented them, I can guarantee he grew more cubes than we will ever see between his researching and his college days. Asshole, horrible business owner, turkey tail cancer curer or not, he is a good cultivator and writer.



Thanks for your insight. I am surprised whenever I see people hating on Stamets on The Shroomery. After seeing a few of his talks, I instantly idolized him and figured other mushroom lovers and hobbyists would do the same.
I can see how you can lose some respect for him due to fungi perfecti. Personally, I don't know a whole lot about it (other than I wanted to buy Mycelium Running from it) so I can't form an opinion on it, but if what you are saying about it is true, then that is disappointing.
Regardless however, I still think people should respect Stamets and listen to the things he has to say. If his claims are exaggerated then I want to see people prove it. Unfortunately, it seems like a lot of scientists ignore mushrooms research too often. I think fungi could make enourmous positive difference.


Quote:

Rooster Cogburn said:
their cultures really suck, they are on the bottom of the chain for commercial cultures.



Which commercial cultures are at the top of the chain?


Sorry that my post is way off the oriinal topic. This is a really good thread though, lot of stuff going on


--------------------
Alice asked the Cheshire Cat, who was sitting in a tree, “What road do I take?”

The cat asked, “Where do you want to go?”

“I don’t know,” Alice answered.

“Then,” said the cat, “it really doesn’t matter, does it?”


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Invisiblec10h12n2o
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Re: Strain Isolations on Agar, Pics and Questions [Re: TedTheHighlighter]
    #23573213 - 08/24/16 03:20 PM (7 years, 5 months ago)

Quote:

TedTheHighlighter said:
After seeing a few of his talks, I instantly idolized him and figured other mushroom lovers and hobbyists would do the same.





this is exactly why:


--------------------

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"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
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Offlinespacechildo
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Re: Strain Isolations on Agar, Pics and Questions [Re: c10h12n2o]
    #23573264 - 08/24/16 03:33 PM (7 years, 5 months ago)

Quote:

c10h12n2o said:
UPDATE: here are a couple of the more rhizo plates i mentioned. one has grown out, and the other 2 are more current. one seems to be an isolate :smile:





the outgrown one? because none of the rest looks isolated if you mean a strain/monoculture and notjust isolated somewhat or whatever they say nowaday :lol:


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Re: Strain Isolations on Agar, Pics and Questions [Re: TedTheHighlighter]
    #23573280 - 08/24/16 03:36 PM (7 years, 5 months ago)

Ted, the best commercial cultures come from Aloha culture bank, Amycel, Sylvaan, and I'm sure I'm missing a few but IMO Aloha is #1. I have bought both spawn KO1 Eryngii(which I saved by taking grains to plates>slants) and have 2 traded cultures from them, shiitake 3782, and their white oyster.
I grow grey oysters mainly for extra cash so I use Amycel 3015, from Amycel, it is the best oyster culture I have found, you can only order it by phone though and send a money order or COD, and takes quite a bit of time to get it, it comes in 20# spawn bags something like a buck a pound, and is WAY worth it if you get into pumping out oysters, but also I have that saved and stored from taking grains to plates>slants, but I would never discourage anyone from buying more spawn when needed.

c10, I have a purchased FP culture, Enoki, and also have a traded Enoki that blew FP's out of the water, and also have a traded FP Shiitake that sucks ass compared to the Aloha one. I don't know anyone who grows FP cultures by choice:shrug: if you do I would love to hear it.
If you guys havn't checked it out the Aloha Culture Bank is just a an awesome website to go check out in general, lot's of information and medicinal studies. http://www.alohaculturebank.com/grain-spawn.html#.V74QjPkrLIU


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Invisiblec10h12n2o
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Re: Strain Isolations on Agar, Pics and Questions [Re: Rooster Cogburn]
    #23573338 - 08/24/16 03:52 PM (7 years, 5 months ago)

no i was thinking the first one might be getting close, if not there. note these are shots of underside of the plates. showed symetrical, even growth on the plate it came from, and is exactly the same on this plate. what specifically makes you so sure its not?

bear in mind these are my first isolations, so im definitely still learning

much obliged rooster, thats some killer info. I havent ordered any cultures in a while, but i love aloha for cheap filter disks! they will definitely be my next vendor for cultures :thumbup:

everything i am currently working with has either come from hawk's eye or trade


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Offlinespacechildo
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Re: Strain Isolations on Agar, Pics and Questions [Re: c10h12n2o]
    #23573349 - 08/24/16 03:55 PM (7 years, 5 months ago)

its far from a perfect circle. how many transfers in are you?


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Re: Strain Isolations on Agar, Pics and Questions [Re: spacechildo] * 1
    #23573386 - 08/24/16 04:07 PM (7 years, 5 months ago)

Tonight I'm making slants for the first time. I don't have parafilm as I've never used it....would micropore tape be a fine substitute for taping around the caps?



Once I PC these I'll put them on the bench at a slight angle and drop clean wedges in once they solidify. I wish they had a wider mouthed, its going to be a pain getting wedges out when I need them again,  but I'm working with what I've got.

I plan on keeping them stored in a ziplock inside of jar with SFD filter inside the fridge.

Tips?


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Re: Strain Isolations on Agar, Pics and Questions [Re: spacechildo]
    #23573417 - 08/24/16 04:16 PM (7 years, 5 months ago)

its perfectly symmetrical to the agar i transferred though, and using calipers to measure from the edge of the transferred agar to the leading edge of the myc it is almost dead even all the way around. this plate is either 11 or 12 transfers in

btw i had been meaning to ask you something spacechildo: when you have a plate that is heavily sectored showing 2 distinct kinds of myc, and there are 3-4 sections of aggressive rhizo myc and 3-4 linear sectors, and some or all of the rhizo sectors look similar, or are even connected, do you transfer all the good looking sectors, even though there is a good chance that some of them are the same? especially late into a series of transfers, like 10-15th plate.

sometimes lately it has seemed like i am transferring the same thing twice on the same plate, what appears to be 4 sectors might only be 1 "sector" that is on top/under another sector that is diving it (not to say that this single "interrupted sector" is an individual strain, of course there could be numerous compatible strains forming the same "sector")

i suppose there is no harm in labeling and testing them all separately, but im working on so much right now that i would like to minimize wasted time/space on testing strains X, Y, and Z against each other only to discover/suspect that they are indeed only one strain.

let me know if i need to clarify anything. i would really like to hear your thoughts on this and potential ways to negate this possibility without missing out on potentially valuable strains

warm regards


ps:re: ben
probably better than nothing, but you should get some parafilm brother! that stuff is the bomb, and a little bit goes a LONG way if you use it right. hell, PM me and i will send you some. I lucked out and found a lab supply store that stocks the old kind that was even better :laugh:


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C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


Edited by c10h12n2o (08/24/16 04:19 PM)


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