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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Strain Isolations on Agar, Pics and Questions [Re: Rooster Cogburn]
#23466992 - 07/22/16 06:17 PM (7 years, 6 months ago) |
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just stick around and you'll both see what we're talking about
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Rooster Cogburn
Ranger

Registered: 07/19/16
Posts: 265
Last seen: 7 years, 2 months
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Re: Strain Isolations on Agar, Pics and Questions [Re: spacechildo]
#23467006 - 07/22/16 06:21 PM (7 years, 6 months ago) |
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Quote:
spacechildo said:
Quote:
c10h12n2o said: just because someone has a shroomery account and knows how to grow cubensis doesnt make them a mycologist on the same level with people like Stamets and countless other academic researchers who devote their lives to the topic, thats just silly/small-minded/arrogant
No, I'm saying that said mycologists have shroomery accounts. Why do they suddenly become a "random hippie" when they log on? And you must know how few studies are actually done on cubensis or other actives, most stuff we get taught about stuff like vert or mycogone comes from universities that publish stuff on agaricus production.
Stamets is one of those few people that had been approved and funded to study cubensis. And I agree Agiricus cultivation is where we have learned so much, If paul wanted to farm cubensis it would look just like his Agiricus cultivation and with his own compost that he spent a good portion of TMC teaching about.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Strain Isolations on Agar, Pics and Questions [Re: Rooster Cogburn]
#23467751 - 07/22/16 10:37 PM (7 years, 6 months ago) |
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TMC is shit compared to the shroomery. There's no arguing that. Notice all the regulars agree it's shit compared to the shroomery. That isn't a coincidence. Shroomery is reviewed by all the members and has an amazing active knowledge base. Something i know stamets would be jealous af to have. When it comes to the cultivation of actives, nobody does it better then this community of OCD fucks.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Strain Isolations on Agar, Pics and Questions [Re: Mad Season]
#23467882 - 07/22/16 11:19 PM (7 years, 6 months ago) |
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Quote:
Mad Season said: nobody does it better then this community of OCD fucks.

TMC was certainly to go-to book in the past for anyone wanting to cultivate anything but Paul definitely stopped using some of the stuff that's in there, just like we did. As space said, there is some outdated info in there, which stamets himself is said to have taken back since then during his seminars. I mean, you have to remember that this book was originally released in the 80's. Anything that old is bound to have some outdated stuff, especially with a practice that hasn't been around that long.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Strain Isolations on Agar, Pics and Questions [Re: Rooster Cogburn]
#23468082 - 07/23/16 12:39 AM (7 years, 6 months ago) |
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Quote:
Rooster Cogburn said: Have you ever seen his grow rooms? They are fucking nuts.
 What "teks" do you think he would be looking into or unaware of?
He grows edibles... Not cubes You could be a edible mushroom farmer your whole life and still fuck up a cube grow your first time, he hasn't grown cubes in decades. Back when TMC was wrote PF cakes and aquariums with drip shields were high tech.
Staments is a great researcher, great grower, but a miserable scientist. He touts mushrooms as a legitimate cure for cancer, and has never cured a single person
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Strain Isolations on Agar, Pics and Questions [Re: bodhisatta]
#23468109 - 07/23/16 12:55 AM (7 years, 6 months ago) |
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That's because he has something to gain by selling medicinal capsules to the public.
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Rooster Cogburn
Ranger

Registered: 07/19/16
Posts: 265
Last seen: 7 years, 2 months
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Re: Strain Isolations on Agar, Pics and Questions [Re: Supalemonhaze]
#23468242 - 07/23/16 02:18 AM (7 years, 6 months ago) |
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I bet the entire north west was high as kites the entire time Paul was in school. How do you think he paid for that prestigious schooling I'm joking, I see what you mean though, as far as gourmet mushroom cultivation is concerned his book GGMM is still golden and relevant for anyone diving into semi commercial or commercial cultivation.
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weetsie
unlicensed tub surgeon



Registered: 05/08/11
Posts: 572
Loc: United Kingdom
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Re: Strain Isolations on Agar, Pics and Questions [Re: Rooster Cogburn]
#23468297 - 07/23/16 03:47 AM (7 years, 6 months ago) |
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Scraped up at least half a square inch of spores and dumped them in a pile on a plate.

Feeling confident 
EDIT: for anyone that's intrested...
Quote:
weetsie said:
Quote:
Supalemonhaze said:
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weetsie said: Even when you don't dilute spores and start with a big black dot for your first plate you can still get isolates in less than 5 transfers.
I'd like to see you try and pull this off and document it.
I'll take you up on that.
Watch this space.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Strain Isolations on Agar, Pics and Questions [Re: weetsie]
#23468411 - 07/23/16 06:48 AM (7 years, 6 months ago) |
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This ought to be interesting. This deserves a new thread.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Strain Isolations on Agar, Pics and Questions [Re: Supalemonhaze]
#23468817 - 07/23/16 10:18 AM (7 years, 6 months ago) |
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Most people get something looks like the PEU plate on the right
 Oh man I got an isolate.
Nope keep going more than a few more transfers
You get plates to look like the two on the right(LOI)
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Strain Isolations on Agar, Pics and Questions [Re: bodhisatta]
#23468829 - 07/23/16 10:22 AM (7 years, 6 months ago) |
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Wispy cultures are annoying to isolate, I was going with PE my first time. In the end I decided to go with a more rhizomorphic culture.
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weetsie
unlicensed tub surgeon



Registered: 05/08/11
Posts: 572
Loc: United Kingdom
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Re: Strain Isolations on Agar, Pics and Questions [Re: Supalemonhaze]
#23468846 - 07/23/16 10:26 AM (7 years, 6 months ago) |
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Exactly why I picked EQ, very rhizo every time I have grown it.
-------------------- Active grow logs: Oysters on Straw Pellets Trade list
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Strain Isolations on Agar, Pics and Questions [Re: Mad Season]
#23468943 - 07/23/16 11:00 AM (7 years, 6 months ago) |
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Quote:
Mad Season said: TMC is shit compared to the shroomery. There's no arguing that. Notice all the regulars agree it's shit compared to the shroomery. That isn't a coincidence. Shroomery is reviewed by all the members and has an amazing active knowledge base. Something i know stamets would be jealous af to have. When it comes to the cultivation of actives, nobody does it better then this community of OCD fucks.
I think we are saying the same thing. a 30 yr old book and a modern forum are two VERY different contexts. To say that one is better than the other is ignoring that context. apples and oranges, reishi and cubensis. only by completely missing the point and misconstruing my post (or not reading it) could someone think i said TMC > Shroomery. context is everything, i really dont think there is an argument here
anyone who has read and has the humility and perspective to appreciate the principles covered in TMC will obviously recognize the tremendous value of a public forum that allows people to discuss those principles (and new ones)
BTW thanks to everyone who has taken the time to contribute their knowledge and experience to this thread, especially regarding the questions. and big thanks to everyone who has posted pics and examples (and even experiments!! )for the benefit of others who might have the same questions. i very much appreciate it, and im sure countless others will as well (even if they dont post)
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Strain Isolations on Agar, Pics and Questions [Re: c10h12n2o]
#23471909 - 07/24/16 10:32 AM (7 years, 6 months ago) |
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I didn't mean it directly at you. I meant it in general. Though to say these random hippies who have spent their time on 1 species don't know more than a mycologist who has a generalization of all species is just a perspective. Every other week I'm correcting a person who works in a mycology lab or someone's mycology friend. Every species is different, but on this forum there's lots of people of all different types who collectively know more than any mycologist.
Anyways..
@supale might be a bit late but this is a jar of grains literally covered in bacteria:

I know because it's been sitting on my cupboard thing with the rest of my contaminated shit without being PCd for 2 months now. Also the bacteria kept out all other competitors even tho it was prepared in open air, soaked for 12 hours and never saw any heat.
Grains are almost impossible to tell if they have bacteria until the very end when the mycelium shows you what's happening, and even then it's kinda hard to tell. There's over 200,000 bacterium on every mm of our skin. Especially our hands. Grain masters MUST be clean. Both inside and out. Bacteria also travels on dust particles too. Also yes there is endospores to think about too. Though if you colonize quickly, they won't be a problem.
Edited by Mad Season (07/24/16 11:11 AM)
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Strain Isolations on Agar, Pics and Questions [Re: Mad Season]
#23483958 - 07/27/16 09:40 PM (7 years, 6 months ago) |
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makes perfect sense, thanks mad. exactly, thats what makes this place such an awesome resource for both amateur and professional mycologists alike 
btw
UPDATE:
I have finished a few more series of transfers and the cultures are starting to look MUCH more organized than the earlier plates. Here are a few shots of a few of the ones that look interesting:
20 X (Orissa):
20 Y (Orissa):
 20 Z (Orissa):
100 X (Ecuador):
100 Y (Ecuador):
100 Z (Ecuador):

the pics dont look as good as they do irl, but its amazing how much different they are starting to look from the earlier plates!! I have a lot of others going as well, and am one transfer past this on some AA+ and Orissa.
I cut back the yeast and peptone to McKenna's recipe instead of stamets for these pictured (red agar), and in the current series i am only using half strength MEA (purple agar;, will post pics after further colonization
Any feedback on these? Much obliged my friends 
warm regards
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weetsie
unlicensed tub surgeon



Registered: 05/08/11
Posts: 572
Loc: United Kingdom
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Re: Strain Isolations on Agar, Pics and Questions [Re: c10h12n2o]
#23538160 - 08/13/16 02:33 PM (7 years, 5 months ago) |
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Took 2 transfers from the first plate today.
-------------------- Active grow logs: Oysters on Straw Pellets Trade list
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Strain Isolations on Agar, Pics and Questions [Re: weetsie]
#23563299 - 08/21/16 03:44 PM (7 years, 5 months ago) |
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hello friends, just wanted to post an update to this project
I have several hundred plates going now, going through about 3 sleeves of transfers per day. I am working with lots more than what is posted here, including cordyceps m., p. micro. (plastic digesting), and a bunch of different edibles and medicinals. I absolutely love playing with agar so much faster than gardening
Anyways, here are a few shots of the underside of plates that seem to show the exact same growth on their last 3 transfers (same rate, same shape, same basic characteristics, in the last three series of transfers), so i figure they must be getting close. any feedback is much appreciated, im definitely still getting my feet wet with isolations


a couple of these have been showing the same growth since the 4th and 5th transfer, so they became isolate candidates and i kept transferring them to see if they showed any new sectoring, so maybe i got lucky! though i suppose it would be more lucky if it was more aggressive. I have some really aggressive rhizo cultures in the works right now, i cant wait to see how they look when i get them isolated i will post the better looking cultures as soon as i have some not showing clear sectoring after 2-3 transfers, should have something sexy soon 
though i gotta say, i have been amazed at how often i will have a beautiful rhizo sector that i am confident will be close to an isolate, or at least rhizo on the next plate, only to find that there was indeed a jungle of strains on top of each other, even when transferring a single rhizo tip smaller than a mm. it is also amazing how myc can go from rhizo to linear to tomentose and back again
i really appreciate all the good info guys!! thanks a bunch
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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MysticMoteToter



Registered: 07/26/15
Posts: 2,036
Loc: Who nose.
Last seen: 4 hours, 40 minutes
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Re: Strain Isolations on Agar, Pics and Questions [Re: c10h12n2o]
#23563321 - 08/21/16 03:54 PM (7 years, 5 months ago) |
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nice plates my dude! where did you get the microspora??
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Strain Isolations on Agar, Pics and Questions [Re: MysticMoteToter]
#23563365 - 08/21/16 04:20 PM (7 years, 5 months ago) |
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thanks buddy! i cant wait to post the sexy ones rhizo ones, they just keep sectoring!! will be beautiful though eventually 
I was trading a bunch of cultures getting ready to get back into the hobby, and a few weeks after our trade, a guy sent me p. micro. and cord. m. as a thankyou! i thought it was a pretty fair trade to begin with, but gotta love folks like that i sent him the rest of what i had as a dbl thankyou i think he really dug the AA+
lol now to try to make this sumbeech salt water tolerant and turn some of those plastic islands in the pacific int islands of mushrooms (obviously kidding, mushies hate salt, but a nice thought )
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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MysticMoteToter



Registered: 07/26/15
Posts: 2,036
Loc: Who nose.
Last seen: 4 hours, 40 minutes
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Re: Strain Isolations on Agar, Pics and Questions [Re: c10h12n2o]
#23563394 - 08/21/16 04:28 PM (7 years, 5 months ago) |
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Quote:
c10h12n2o said: lol now to try to make this sumbeech salt water tolerant and turn some of those plastic islands in the pacific int islands of mushrooms (obviously kidding, mushies hate salt, but a nice thought )
It may be possible through genetic modification, like if you took goldenrod or some other salt tolerating species and put that into p. microsp.
That'd be amazing
-------------------- Half Homo Hardly Sapient Overview Effect Fuck War, Feed Birds.
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