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weetsie
unlicensed tub surgeon



Registered: 05/08/11
Posts: 572
Loc: United Kingdom
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Re: Strain Isolations on Agar, Pics and Questions [Re: MysticMoteToter]
#23461223 - 07/20/16 11:41 PM (7 years, 6 months ago) |
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Quote:
MysticMoteToter said: weird, i've got some cubensis Redboy culture that will go tomentose on just about anything with nutrition but can't get it to go rhizo to save my life...however, it's also put out one of my most potent clones....i don't think tomentose growth is indicative of anything other than colonization speed as long as it's not wispy or weak looking 
I think it's only referring to if it happens with old cultures on the same media.
Quote:
Mad Season said: Senescence IME gets wispier, not necessarily more tomentose.. Tomentose is still aggressive and thick, it just doesn't need to stretch as much looking for nutrients, so it gets all thick and bunched up.
I always thought tomentose simply meant cottony, regardless of being strong or weak.
Quote:
noob47 said: What about when it goes from cottony to rhizo? Cause most of my plates when I drop spores is tomentose
I think all spores start like like, never seen any different.
-------------------- Active grow logs: Oysters on Straw Pellets Trade list
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Strain Isolations on Agar, Pics and Questions [Re: weetsie]
#23461447 - 07/21/16 02:22 AM (7 years, 6 months ago) |
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Quote:
weetsie said: Even when you don't dilute spores and start with a big black dot your first plate you can still get isolates in less than 5 transfers.
I'd like to see you try and pull this off and document it. Have you ever even isolated a culture? From this sentence I get the idea that you never really tried it. Even with transfers not even a mm wide, it would still take a lot of transfers to get isolates with a normal spore inoculation (without dilution).

That transfer is what my regular isolation transfer looks like. It's about 1mm wide(if that) and as long as the agar's depth. It's basically impossible for me to take transfers smaller than this. It's actually annoying sometime to pick the transfer up and deposit it on the new plate due to being so small. Still, I've been isolating this variety for no less than 3 months.
And for the sake of not confusing anyone, just because a culture goes from rhizo to cottony, it doesn't mean it's senescening. It takes an awful lot of cell divisions and expansion for a culture to senescene. No one really knows how much agar transfers it takes for a culture to grow old but it's safe to say that no one here will ever experience that through agar transfers alone.
@Mad season: So you only inoculate a grain jar with cultures that grow at the same rate? I get that the one you labelled as "disorganized" is not a good candidate for inoculation, especially the one at the bottom but I constantly get sectors that look clean and organized but grow at different speeds. Never thought of that as a problem as long as the strands look organized and flat.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Strain Isolations on Agar, Pics and Questions [Re: Supalemonhaze]
#23461552 - 07/21/16 03:27 AM (7 years, 6 months ago) |
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Most of us, the vast majority, will never even experience senescence in any culture
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Strain Isolations on Agar, Pics and Questions [Re: bodhisatta]
#23461555 - 07/21/16 03:30 AM (7 years, 6 months ago) |
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Quote:
Trusted Cultivator said: Most of us, the vast majority, will never even experience senescence in any culture

Most hobbyists will never even experience it, the ones who do normally do so from excess G2G's.
hey bodhi, how much transfers would you say it takes to get a culture to grow old? Close to a thousand maybe? Or not even?
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: Strain Isolations on Agar, Pics and Questions [Re: Supalemonhaze]
#23461560 - 07/21/16 03:34 AM (7 years, 6 months ago) |
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What is that when the myc gets old and useless?
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Strain Isolations on Agar, Pics and Questions [Re: Boogieman47]
#23461567 - 07/21/16 03:38 AM (7 years, 6 months ago) |
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Yes. Excess G2Gs/expanded mycelium is usually the cause of it. Mycelium growing on a 2d surface(agar) is senescening at a much slower rate than mycelium growing in a 3d manner like it would in grain jars and bulk subs. This is entirely the reason why they say not to G2G a grain jar for more than 3-5 times.
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: Strain Isolations on Agar, Pics and Questions [Re: Supalemonhaze]
#23461577 - 07/21/16 03:49 AM (7 years, 6 months ago) |
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So what if you did ten jars to 100 jars and so on the third to fifth time it will be pretty useless ? Even if you got 1000 jars ?
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Strain Isolations on Agar, Pics and Questions [Re: Boogieman47]
#23461583 - 07/21/16 03:53 AM (7 years, 6 months ago) |
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No, you can do g2g safely for a few times but they don't ever recommend going over 5 times. Some won't recommend going over 3. The mycelium will still be young enough to colonize the bulk and give a normal healthy flush though. It takes more G2Gs to get senescene, 3-5 times is being safe rather than sorry.
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: Strain Isolations on Agar, Pics and Questions [Re: Supalemonhaze]
#23461584 - 07/21/16 03:55 AM (7 years, 6 months ago) |
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Right I feel you man makes since
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Strain Isolations on Agar, Pics and Questions [Re: Boogieman47]
#23461845 - 07/21/16 07:28 AM (7 years, 6 months ago) |
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Quote:
noob47 said: you man makes since

TMC is over 30 yrs old dude, gotta read it like a bible and take everything with a grain of salt.
Best way to learn how stuff works is to grow, simple as that.
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weetsie
unlicensed tub surgeon



Registered: 05/08/11
Posts: 572
Loc: United Kingdom
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Re: Strain Isolations on Agar, Pics and Questions [Re: Supalemonhaze]
#23462192 - 07/21/16 09:51 AM (7 years, 6 months ago) |
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Quote:
Supalemonhaze said:
Quote:
weetsie said: Even when you don't dilute spores and start with a big black dot your first plate you can still get isolates in less than 5 transfers.
I'd like to see you try and pull this off and document it.
I'll take you up on that.
Watch this space.
-------------------- Active grow logs: Oysters on Straw Pellets Trade list
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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Re: Strain Isolations on Agar, Pics and Questions [Re: weetsie]
#23462228 - 07/21/16 10:02 AM (7 years, 6 months ago) |
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-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Strain Isolations on Agar, Pics and Questions [Re: mupetmower]
#23462292 - 07/21/16 10:27 AM (7 years, 6 months ago) |
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Quote:
mupetmower said:

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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Strain Isolations on Agar, Pics and Questions [Re: Supalemonhaze]
#23462433 - 07/21/16 11:25 AM (7 years, 6 months ago) |
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yeah lets just hope senescense doesnt make your isolates start sectoring again this time!
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lukehighwalker710
Cloud 9 Dweller



Registered: 03/04/16
Posts: 609
Loc: out there
Last seen: 6 years, 22 days
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Re: Strain Isolations on Agar, Pics and Questions [Re: spacechildo]
#23462517 - 07/21/16 12:11 PM (7 years, 6 months ago) |
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Pulling up a chair
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Highwalker
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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If I had a dollar for every time a noob said he'd prove something wrong and even went as far as updating his thread I'd still be a poor son of a bitch!
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lukehighwalker710
Cloud 9 Dweller



Registered: 03/04/16
Posts: 609
Loc: out there
Last seen: 6 years, 22 days
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Re: Strain Isolations on Agar, Pics and Questions [Re: spacechildo]
#23462536 - 07/21/16 12:20 PM (7 years, 6 months ago) |
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Quote:
spacechildo said: If I had a dollar for every time a noob said he'd prove something wrong and even went as far as updating his thread I'd still be a poor son of a bitch!
right? At least update us that it didn't work out don't just dissapear lmao
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Highwalker
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Rooster Cogburn
Ranger

Registered: 07/19/16
Posts: 265
Last seen: 7 years, 2 months
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Re: Strain Isolations on Agar, Pics and Questions [Re: spacechildo]
#23462940 - 07/21/16 03:19 PM (7 years, 6 months ago) |
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Quote:
spacechildo said:
Quote:
noob47 said: you man makes since

TMC is over 30 yrs old dude, gotta read it like a bible and take everything with a grain of salt.
Best way to learn how stuff works is to grow, simple as that.
Out of curiosity, what parts of TMC do you feel are worth taking with a grain of salt? I own the the newest revised edition as well as GGMM. Besides coir and small scale fruiting chambers, what is it missing or has wrong?
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Strain Isolations on Agar, Pics and Questions [Re: Rooster Cogburn]
#23463032 - 07/21/16 03:52 PM (7 years, 6 months ago) |
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oh man... where to begin? everything on lights/darkness, probably most if not all the charts, temps, overlay, basically just remember mush cult is a rather new thing and a lot of what was written in stone has now been disproved. doubt everything, try everything, and if you got a theory try to disprove it rather than prove it, science style
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: Strain Isolations on Agar, Pics and Questions [Re: spacechildo]
#23463076 - 07/21/16 04:05 PM (7 years, 6 months ago) |
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Quote:
spacechildo said:
Quote:
noob47 said: you man makes since

TMC is over 30 yrs old dude, gotta read it like a bible and take everything with a grain of salt.
Best way to learn how stuff works is to grow, simple as that.
Trust me I'm going to buy it but I have grown maybe not as successful as some of you guys on here I've never done that many g2g because I have 6 types of cubes and three clones on agar with like 75 pasty plates I don't know what you're referring to? Space
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