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OfflineGaleenet
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Registered: 05/19/16
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Last seen: 7 years, 1 month
Is it all contam ? [liquid culture]
    #23458235 - 07/20/16 01:14 AM (7 years, 6 months ago)

Started a few cultures from spores one week ago, first time.

I suppose it's all contam in this jar ?



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OfflineFruit Cakes
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Registered: 06/30/16
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Re: Is it all contam ? [liquid culture] [Re: Galeenet]
    #23458265 - 07/20/16 01:39 AM (7 years, 6 months ago)

Do not get your hopes down, it almost looks promising, but i am noob. I have seen this look alike growth on jars, it could just be that it is liquid culture and it grows like that lol - but i swear if i get harassed for trying to start a convo and giving my thoughts ima be sooo mad lol ,i am not even suggesting waht to do or what it is, simply my thoughts lmfaooo, so plz wait for an experienced shroomer to answer this ahaha but like i said dont get ya hopes down lol


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Offlinelocallorax
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Re: Is it all contam ? [liquid culture] [Re: Galeenet]
    #23458266 - 07/20/16 01:40 AM (7 years, 6 months ago)

looks healthy because the water is clear. but you won't know for sure until you test it. that's what everyone is going to tell you


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Invisiblec10h12n2o
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Re: Is it all contam ? [liquid culture] [Re: Galeenet]
    #23458269 - 07/20/16 01:40 AM (7 years, 6 months ago)

what species and what technique did you follow? its hard to tell one species from another in a liquid culture just from a pic, and if its multispore (rather than from an isolated culture) it could have quite a bit of variety. looks good but we definitely need more info to make a good guess


Edited by c10h12n2o (07/20/16 01:41 AM)


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OfflineGaleenet
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Re: Is it all contam ? [liquid culture] [Re: c10h12n2o]
    #23458363 - 07/20/16 03:01 AM (7 years, 6 months ago)

Sorry for lack of details.

It was a LC directly from home made spore print, PC Mexican.
4 jars, 2 of them are showing growths like that. (each jar from a different spore print)

I also have another 4 jars from a bought spore syringe PC Golden Teacher.


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InvisibleSupalemonhaze
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Registered: 10/02/15
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Re: Is it all contam ? [liquid culture] [Re: Galeenet]
    #23458572 - 07/20/16 06:17 AM (7 years, 6 months ago)

Nice, not only did you inoculate an LC with spores (which is super risky) but you also did so with a homemade print (which are normally dirtier than a vendor syringe would be on average).

I mean yeah, it could be that it's clean but honestly, I wouldn't count on it. Inoculate an agar plate before you commit on using that thing on a bunch of jars. LC is pretty risky as it is, using spores on it is just asking for trouble.

BTW, you can never tell if an LC is clean just by looking at it. You can sometimes tell that it's dirty but you can never tell if it's clean. A seemingly clean LC can have some really sneaky shit inside it.


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OfflineGaleenet
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Re: Is it all contam ? [liquid culture] [Re: Galeenet]
    #23458605 - 07/20/16 06:39 AM (7 years, 6 months ago)


that's the top view.
seems like contams on the top ?
might be mycelium in the bottom


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OfflineGaleenet
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Re: Is it all contam ? [liquid culture] [Re: Supalemonhaze]
    #23458607 - 07/20/16 06:41 AM (7 years, 6 months ago)

Quote:

Supalemonhaze said:
Nice, not only did you inoculate an LC with spores (which is super risky) but you also did so with a homemade print (which are normally dirtier than a vendor syringe would be on average).




I like to live dangerously :p

Good to know however.

I'm running LC from home made spore prints, LC from spore syringe, Grains from spore syringe, and am betting a few LC will make it to inoculate more grains jars. If I have too much successful grain jars, I will try bulk, if I don't have any, I have a growkit growing as a fallback.

Just back home for 2 months, so I have to shoot on all cylinders before going back abroad away from my tools.


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InvisiblePastywhyteMDiscord
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Re: Is it all contam ? [liquid culture] [Re: Galeenet]
    #23458608 - 07/20/16 06:43 AM (7 years, 6 months ago)

Quote:

Galeenet said:

that's the top view.
seems like contams on the top ?
might be mycelium in the bottom




That is totally fucked. I wouldn't even waste material testing it.


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InvisibleSupalemonhaze
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Registered: 10/02/15
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Re: Is it all contam ? [liquid culture] [Re: Galeenet]
    #23458609 - 07/20/16 06:44 AM (7 years, 6 months ago)

Is that a different colour at the top? That thing looks fucked up anyway, even from the look of the mycelium floating on top. Pic is absolutely garbage though, there is no telling what kind of mold that is.

You should either learn agar or stick to spores to grains/cakes. LC made from spores is a disater waiting to happen, especially with a homemade print.

If you are relatively new to the hobby, best thing you can do for yourself is forget about LCs. LC is an advanced technique, even with the best tools at your disposal.


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InvisibleSupalemonhaze
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Re: Is it all contam ? [liquid culture] [Re: Supalemonhaze]
    #23458614 - 07/20/16 06:48 AM (7 years, 6 months ago)

:lol: @ living dangerously. More like living stupidly if you are going to keep doing that. Doing it once to see how it goes is one thing, but if you keep doing that, you obviously don't want to grow shrooms successfully.  If you want to inoculate with spores and have relatively "good" results, inoculate straight to grain and don't g2g the jars.

Spores are inherently dirty, homemade ones even more. Inoculating LCs with homemade spores is a recipe for constant failure. It's just a waste of time, energy and material.


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OfflineFruit Cakes
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Re: Is it all contam ? [liquid culture] [Re: Pastywhyte]
    #23458624 - 07/20/16 06:56 AM (7 years, 6 months ago)

The bacteria which is growing on top is were it breaths, but thre is bacteria ridden through out the whole agar, lmfao the mold is breathing thru the top of the agar which is thriving on top of the agar, so bascialy the actual contamination is all inside of agar, just u can see were it breaths, DONT TAKE thIS NOOBS WORD FOR IT THO  -I COULD BE WRONG AS HELL


Edited by Fruit Cakes (07/20/16 07:01 AM)


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InvisiblePastywhyteMDiscord
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Re: Is it all contam ? [liquid culture] [Re: Fruit Cakes]
    #23458631 - 07/20/16 06:58 AM (7 years, 6 months ago)

Quote:

Fruit Cakes said:
The bacteria is breathing thru the culture which is thriving on top of the agar, so bascialy the actual contamination is all inside of agar, just u can see were it breaths, DONT TAKE thIS NOOBS WORD FOR IT THO  -I COULD BE WRONG AS HELL




This makes no sense whatsoever.


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InvisibleSupalemonhaze
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Re: Is it all contam ? [liquid culture] [Re: Fruit Cakes]
    #23458632 - 07/20/16 06:58 AM (7 years, 6 months ago)

Quote:

Fruit Cakes said:
The bacteria is breathing thru the culture which is thriving on top of the agar, so bascialy the actual contamination is all inside of agar, just u can see were it breaths, DONT TAKE thIS NOOBS WORD FOR IT THO  -I COULD BE WRONG AS HELL




:thatsinteresting:


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OfflineFruit Cakes
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Re: Is it all contam ? [liquid culture] [Re: Supalemonhaze]
    #23458633 - 07/20/16 06:59 AM (7 years, 6 months ago)



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Offlinemrmazdarx9
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Re: Is it all contam ? [liquid culture] [Re: Fruit Cakes]
    #23458716 - 07/20/16 07:48 AM (7 years, 6 months ago)

Quote:

Fruit Cakes said:
https://www.shroomery.org/forums/images/icons/heart.gif



Awesome emoji :facepalm:


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OfflineFruit Cakes
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Re: Is it all contam ? [liquid culture] [Re: mrmazdarx9]
    #23458719 - 07/20/16 07:52 AM (7 years, 6 months ago)



Edited by Fruit Cakes (07/20/16 07:52 AM)


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OfflineBobabouy
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Re: Is it all contam ? [liquid culture] [Re: Fruit Cakes]
    #23458752 - 07/20/16 08:09 AM (7 years, 6 months ago)

wrecked.


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InvisibleTimmy Meow
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Re: Is it all contam ? [liquid culture] [Re: Galeenet]
    #23458787 - 07/20/16 08:20 AM (7 years, 6 months ago)

Quote:

Fruit Cakes said:
Do not get your hopes down, it almost looks promising, but i am noob. I have seen this look alike growth on jars, it could just be that it is liquid culture and it grows like that lol - but i swear if i get harassed for trying to start a convo and giving my thoughts ima be sooo mad lol ,i am not even suggesting waht to do or what it is, simply my thoughts lmfaooo, so plz wait for an experienced shroomer to answer this ahaha but like i said dont get ya hopes down lol



Quote:

Fruit Cakes said:
The bacteria which is growing on top is were it breaths, but thre is bacteria ridden through out the whole agar, lmfao the mold is breathing thru the top of the agar which is thriving on top of the agar, so bascialy the actual contamination is all inside of agar, just u can see were it breaths, DONT TAKE thIS NOOBS WORD FOR IT THO  -I COULD BE WRONG AS HELL




Are you on shrooms right now? :lol: :shrug:

Quote:

Galeenet said:

that's the top view.
seems like contams on the top ?
might be mycelium in the bottom




Chuck that thing and never look back, please.


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Offlinelocallorax
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Registered: 07/27/12
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Last seen: 4 years, 3 months
Re: Is it all contam ? [liquid culture] [Re: Timmy Meow]
    #23458801 - 07/20/16 08:24 AM (7 years, 6 months ago)

Yeah, toss it. Didn't see the floating stuff


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