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b1blazin
The Transcendent


Registered: 06/16/12
Posts: 399
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Cordyceps
#23457453 - 07/19/16 08:19 PM (7 years, 6 months ago) |
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just wondering if anbody has any updated info on cordyceps and primarily on cordyceps sinensis (cs-4) and its cultivation techniques. Been through numerous articles and on here all day looking found a few hit and misses but nothing definitive in the last few years. I would like to obtain culture but first want to grasp on the process.
Things ive found so far:
1 2 3 I appreciate ANY info and contributions
Edited by b1blazin (07/19/16 11:50 PM)
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weetsie
unlicensed tub surgeon



Registered: 05/08/11
Posts: 572
Loc: United Kingdom
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I got a CS-4 culture recently. It's incredibly quick to cultivate on grain.
If you shake a jar of grain inoculated with a wedge of CS-4, even if it's only 3% colonized it will recover as if it had been shaken at 100%. It's as if the mycelium breaks up into tiny fragments that recover rapidly.
If you swab an agar plate with CS-4 mycelium the entire plate will be dense with mycelium in just a few days. It's weird stuff.
-------------------- Active grow logs: Oysters on Straw Pellets Trade list
Edited by weetsie (07/22/16 02:00 AM)
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Gr0wer
always improving



Registered: 09/16/03
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Loc: El Paso, TX
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Re: Cordyceps [Re: weetsie]
#23464396 - 07/21/16 11:08 PM (7 years, 6 months ago) |
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grow it on organic brown rice and harvest the myc after 6 weeks min.
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Eywa_devotee
Goddess Worshiper


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Re: Cordyceps [Re: weetsie] 1
#23464541 - 07/21/16 11:59 PM (7 years, 6 months ago) |
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Cordyceps needs a lot of protein to fruit properly. It's cultivation is definitely an "advanced mycology" subject.
First make an agar plate, malt yeast soy agar formulations are best. This is to expand your culture and to have a reserve if something goes wrong. Incubate the plate at about 75 deg F. When the plate is covered with fluffy mycelium, cut a wedge and make a LC using a light malt extract with some protein source (I used some soy based baby formula since it can be pasteurized without congealing like eggs do) added to it once it gets obviously started. Keep the temperature near 75 deg also.
Note, this LC needs very active aeration with a small sterile air bubbler setup, so you'll have to get creative... This same technique can be used to make ergot LC btw. Let it brew until it has very obvious mycelial growth, white strands floating in it.
Get some dried mealworms and mix it with the grains in a 70 grain to 30 mealworm dry weight ratio. The grain can be anything from WBS to rice to rye, but avoid corn and oats. Other than that, hydrate and process as if you were growing cubes. Knock 'em up with your LC and shake well to mix the LC in.
Allow to colonize at temperatures in the 65 to 75 degree F range and shake periodically to keep it from turning into a large monolithic mass. When all the grains and worms are well covered with fluffy mycelium it's ready. Scoop out the jars and case with some moist vermiculite and fruit in a tray in tub setup or even directly from the jars or bags. Like cubes, the extra moisture from casing will get larger fruits and longer duration of fruiting.
I cannot stress enough that the biggest fruiting trigger is light, but temperature and FAE are also important for this fungus. The optimal temperature to fruit is 77 degrees plus or minus a couple degrees. Better to err on the cooler side if possible. Temperatures over 90 will stunt and kill the mycelium. It will fruit prolifically and pretty chaotically, you'll get gnarly looking orange stalagmites. Hope this helps, and have fun.
-------------------- "Love one another." "To Love is to know me." "Love is the Law, Love under Will." "In Compassion, all sorrows end." Regardless of the Master, the message is the same- Choose love and you shall live, Choose Fear and you shall die. Help bring peace to this Earth: Love one another, and serve others before yourself.
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Lennybernadino
Amazon grower


Registered: 09/16/09
Posts: 770
Loc: Iquitos, Peru
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How do you calculate the mealworm's dry weight? Are the mealworm's already dry?
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Eywa_devotee
Goddess Worshiper


Registered: 10/04/10
Posts: 1,088
Loc: State of Confusion, Arkan...
Last seen: 3 years, 7 months
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I used the pre dried ones used for chicken treats. If you use freshly killed, then just multiply by 9.
-------------------- "Love one another." "To Love is to know me." "Love is the Law, Love under Will." "In Compassion, all sorrows end." Regardless of the Master, the message is the same- Choose love and you shall live, Choose Fear and you shall die. Help bring peace to this Earth: Love one another, and serve others before yourself.
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Gr0wer
always improving



Registered: 09/16/03
Posts: 6,056
Loc: El Paso, TX
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Ive also been told by multiple sources you need a fresh wild culture and it will only fruit for a limited time/generations before the culture will just stop fruiting.
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b1blazin
The Transcendent


Registered: 06/16/12
Posts: 399
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Re: Cordyceps [Re: Gr0wer]
#23472509 - 07/24/16 02:32 PM (7 years, 6 months ago) |
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OK guys CHECK THIS OUT so seems to be little difference between C.s and C.m. hopefully with whats provided and tid bit knowledge ive found so far im just going to have to get a culture and try. Ive been reluctant as of yet as the price is scaring me. WEETSIE, did you get your cs-4 from aloha or a reputable source? cost?
I was really hoping someone had an updated tek here on the shroomery for such. If things work out i will make one myself to put up here. Ive sent alot of emails today. thanks guys feel free to add and ask
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weetsie
unlicensed tub surgeon



Registered: 05/08/11
Posts: 572
Loc: United Kingdom
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Quote:
b1blazin said: WEETSIE, did you get your cs-4 from aloha or a reputable source? cost?
Nope, got it from myceliups so you could ask him. The culture certainly looks like CS-4, going off photos online.
-------------------- Active grow logs: Oysters on Straw Pellets Trade list
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drake89
Mushroom Magnate



Registered: 06/26/11
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Re: Cordyceps [Re: Gr0wer]
#23473821 - 07/24/16 09:46 PM (7 years, 6 months ago) |
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Quote:
Gr0wer said: Ive also been told by multiple sources you need a fresh wild culture and it will only fruit for a limited time/generations before the culture will just stop fruiting.
Cs4 is not a fruiting strain so don't bother. The trade paper about it reads like aloha fruits it but it's just over grown grain. Like 2 or 3 months and it's 90+% mycelium. As grower said it'd best to find a wild clone and it will fruit off only grain like rice or sorghum etc.
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b1blazin
The Transcendent


Registered: 06/16/12
Posts: 399
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Re: Cordyceps [Re: drake89]
#23492667 - 07/30/16 01:46 PM (7 years, 5 months ago) |
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well i highly doubt ill ever be able to find one and true cs-4 from aloha is 500? i just want to get both and try very little info about them in general. ive asked a buncha peeps. im not sure i truely understand how it isnt a fruiting strain when it grows the worm like bodies
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Gr0wer
always improving



Registered: 09/16/03
Posts: 6,056
Loc: El Paso, TX
Last seen: 5 years, 10 months
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Its not a fruiting strain in that only fresh wild cultures will fruit for a few months before the cultures age will no longer allow fruiting. This is what i have heard from multiple sources.
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drake89
Mushroom Magnate



Registered: 06/26/11
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Re: Cordyceps [Re: Gr0wer]
#23495181 - 07/31/16 10:41 AM (7 years, 5 months ago) |
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Quote:
Gr0wer said: Its not a fruiting strain in that only fresh wild cultures will fruit for a few months before the cultures age will no longer allow fruiting. This is what i have heard from multiple sources.
CS4 is a hybrid I'm pretty sure, like it was made in the lab. I think the whole draw of Cordyceps sinensis is that it only grows on the Himalayan mountains. at least that's what it seems to me. I figured if you could grow it inside some crafy chinese person would be doing it, given the high price.
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: Cordyceps [Re: drake89]
#23505065 - 08/03/16 10:33 AM (7 years, 5 months ago) |
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The cs4 strain is 30 years old and isn't even true cordyceps sinensis but an anamorph of true sinensis, which aloha doesn't even sell to the public.
It is not a fruiting strain but the mycelium is extremely beneficial.
If you want a fruiting strain, try to acquire cordyceps militaris k1 which is supposed to be a solid fruiter.
I have a few over colonized jars of cs4 that I am still hesitant to use because of the overwhelming ammonia smell of the mycelium.
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anthiawe
friendly stranger


Registered: 05/18/16
Posts: 652
Last seen: 2 years, 7 months
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was doing research this morning on cordyceps, it can be fruited on supplemented rice in jars, but yes that is C. militaris only. apparently its better for medicinal compounds to be harvested from mycelium rather than the fruits.
i didn't copy the link but i found this: This is a summary of the recipe and the methodology used on my first experience with Cordyceps:
Cook peeled and sliced potatoes in mineral water or distilled water on low heat until tender. Filter the broth through a sieve and discard the potatoes.
Potato Broth
500 g potatoes (peeled and sliced thin) 5000 ml water (distilled or mineral water)
Prepare the following nutrient solution using the potato broth prepared above:
Nutrient Solution
200 ml potato broth 2 g KH2PO4 1 g MgSO4 1 g C6H14N2O7 (Ammonium citrate) 30 g glucose (dextrose) 3 g Soybean peptone 50 mg vitamin B1 (thiamine)
Dilute all the above components with water by adjusting the volume to 1000 ml
Measure 35 ml of nutrient solution and pour into wide mouth glass jars. Weigh the rice grains (30g) and put them in the jars along with the nutrient solution. Close the glass flask with a metal lid with a felted disc under the lid. You do not need a hole in the metal lid, only the filter under the lid is enough for the exchange of air during the growth of the mycelium.
Per jar :
35 ml nutrient solution 30 g rice (polished or brown rice)
After sterilization, inject approximately 5 ml of the Liquid culture of Cordyceps militaris into every glass jar (use a micropipette with sterilized tips or even a stainless spoon sterilized on the flame of a Bunsen burner). Incubate in the dark (25ºC) until the mycelium cover all the grains.
Primordia initiation
Start a one week treatment for the initiation of primordia by putting the flasks on the refrigerator at night and leaving them at an illuminated area during the day (18-25 ºC), without direct sunlight. After the appearance of primordia , keep the flasks in a 18-23ºC environment with indirect light until the mushrooms develop to full size.
or check out
https://www.shroomery.org/forums/showflat.php/Number/11986207
-------------------- TEK compendium
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b1blazin
The Transcendent


Registered: 06/16/12
Posts: 399
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im still researching this good input and is helpful
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