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lone_psychonaut
Pawn



Registered: 09/27/12
Posts: 95
Loc: Somewhere in the depths o...
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Ms vs LC for agar
#23456101 - 07/19/16 12:41 PM (7 years, 6 months ago) |
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I'm looking to begin my strain isolation on agar. I have a ms syringe. I've read that it is better to inoculate the agar with a LC over a ms because you can jump start the mycelium with nutrients in the LC and ultimately achieve higher alkaloid yields. Is this true?? I can't find anymore information on this but this is the thread I read this from https://www.shroomery.org/forums/showflat.php/Cat/0/Number/514231/page/2/fpart/all/vc/1
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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That thread is dated 14 years ago and the OP doesn't know what the fuck he's talking about.Potency is defined by genetics. If you want potent shrooms, you need to clone a potent shroom.
Start isolation by inoculating a soft agar recipe with spores, transfer a bunch of times until you see definite sectoring and once you start getting close to getting isolates, take multiple transfers from multiple sectors. You need a bunch of isolates if you want to find something worth keeping. Once you have like 10+ isolates, start testing them one by one and compare them. Things to look for are good yields, good potency, ease of harvest, contam resistance, size, looks etc.
RR used to recommend that one should get at least 20 isolates if he wants to have the best chance of finding something good. Isolation is a lot more work than you first make it out to be. You should clone at first and do isolation as a side project.
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lone_psychonaut
Pawn



Registered: 09/27/12
Posts: 95
Loc: Somewhere in the depths o...
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Thank you, I was skeptical myself. So there's no added benefit in a LC already introduced to nutrients vs ms syringe?
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lukehighwalker710
Cloud 9 Dweller



Registered: 03/04/16
Posts: 609
Loc: out there
Last seen: 6 years, 22 days
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I believe it should be added that for stubborn syringes like I received, an LC can be a useful way to get germination to take to agar and transfer away from. I have an ATL syringe that must be highly bacterial (but unlike the other bacteria I've seen that's been yellow, this one is pink) and won't germinate straight to agar. Every time I try to put the spores on a plate it gets overrun with contam and no germination. However, my LC has a decent culture so I took that to agar and have some germination I can try to transfer away from. So LC to agar could still be useful. However, in the idea that added nutes will boost potency, the answer is no definitely not.
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Highwalker
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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The MS syringe is for inoculating stuff. What you are saying is wheter or not you should inoculate an LC (which has nutrients) or an agar plate (which also has nutrients). You can see how it won't really matter as both of those have nutrients.
Going from spores straight to LC is a pretty good way to get something sneaky hidden in your culture. You should always start with agar.
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lone_psychonaut
Pawn



Registered: 09/27/12
Posts: 95
Loc: Somewhere in the depths o...
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I see, that makes sense now!! Thank you!
-------------------- Feed your head...
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