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OfflinePsychsense
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Cyber LC Tek possible with smaller jar?
    #23454708 - 07/18/16 11:40 PM (7 years, 6 months ago)

I've always used Cybers Liquid Culture tek with great results!
Usually colonizing my jars a week faster then just spore inoculation.

https://www.shroomery.org/10477/Cybers-Liquid-Culture-Tek

The tek calls for using pint jars (16 fl oz).

I have used larger quart jars (32 fl oz) in the past successfully to create LC jar, using double the corn syrup amount (4 teaspoons).

My question is.. would it possible to use a smaller half pint jar(8 fl oz) to accomplish the same goal / amount of mycelium? Using only 1 teaspoon of corn syrup?

I know i could go buy more pint jars.. but if this is possibility it would save a lot of room in my fridge, where i could store and stack multiple strained LC's.


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Shared Trip Reports (with Empire420): December 25th, 2012 | 2 Day, 16mg DOC Trip report.


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Invisiblewowimflabbergasted
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Re: Cyber LC Tek possible with smaller jar? [Re: Psychsense]
    #23454763 - 07/19/16 12:20 AM (7 years, 6 months ago)

Yeah


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InvisibleSupalemonhaze
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Re: Cyber LC Tek possible with smaller jar? [Re: wowimflabbergasted]
    #23454950 - 07/19/16 03:41 AM (7 years, 6 months ago)

Sure, people grow LCs in syringes. You can do it pretty much in any container if you manage to keep it sterile.


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OfflinePsychsense
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Re: Cyber LC Tek possible with smaller jar? [Re: Supalemonhaze]
    #23461212 - 07/20/16 11:33 PM (7 years, 6 months ago)

Awesome. Thanks guys.
I inoculated some B+ and Ecuadors strains.

Now the waiting game.
:yeahthatsright:


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Who's got the herb when I'm dry?

Shared Trip Reports (with Empire420): December 25th, 2012 | 2 Day, 16mg DOC Trip report.


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Invisiblewowimflabbergasted
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Re: Cyber LC Tek possible with smaller jar? [Re: Psychsense]
    #23461250 - 07/20/16 11:53 PM (7 years, 6 months ago)

:goodluck:


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InvisibleSupalemonhaze
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Re: Cyber LC Tek possible with smaller jar? [Re: wowimflabbergasted]
    #23461418 - 07/21/16 02:03 AM (7 years, 6 months ago)

I hope you used agar to inoculate those. I just realised the "cyber tek" calls for a spore syringe. Might as well be called "stoneage tek".


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OfflinePsychsense
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Re: Cyber LC Tek possible with smaller jar? [Re: Supalemonhaze]
    #23461587 - 07/21/16 04:01 AM (7 years, 6 months ago)

Quote:

Supalemonhaze said:
I hope you used agar to inoculate those. I just realised the "cyber tek" calls for a spore syringe. Might as well be called "stoneage tek".




No, i inoculated with spores.
I haven't had any problems for the past 4 years with this method.

Why do you believe cybers method is a "stoneage tek"?


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Who's got the herb when I'm dry?

Shared Trip Reports (with Empire420): December 25th, 2012 | 2 Day, 16mg DOC Trip report.


Edited by Psychsense (07/21/16 04:24 PM)


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InvisibleSupalemonhaze
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Re: Cyber LC Tek possible with smaller jar? [Re: Psychsense]
    #23461630 - 07/21/16 04:36 AM (7 years, 6 months ago)

Because anyone who wants a semi-reliable LC uses agar nowadays. If you really have been using LCs made with spores successfully, without a single hitch for 4 years, you are either blessed or bullshitting.


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Offlinetump
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Re: Cyber LC Tek possible with smaller jar? [Re: Supalemonhaze]
    #23461684 - 07/21/16 05:40 AM (7 years, 6 months ago)

I believe him. Without lid failure my lc form spores that my vendors sell work perfectly. Even the guy that ran Hawkeyes or mushroom that spores form syrices to lc are fine on lc. Spore form print need agar.


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Invisiblewowimflabbergasted
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Re: Cyber LC Tek possible with smaller jar? [Re: Supalemonhaze]
    #23461864 - 07/21/16 07:36 AM (7 years, 6 months ago)

Quote:

Supalemonhaze said:
Because anyone who wants a semi-reliable LC uses agar nowadays. If you really have been using LCs made with spores successfully, without a single hitch for 4 years, you are either blessed or bullshitting.



All he needs is a clean syringe and a PC'd jar of water/nutes. It's not impossible, far from it...I understand it is safer to use agar and stuff but spores still work fine! May not be 100% clean all the time but some ppl don't give a shit.

You ever make LC supa?


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Offlinespacechildo
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Re: Cyber LC Tek possible with smaller jar? [Re: tump]
    #23461875 - 07/21/16 07:40 AM (7 years, 6 months ago)

Quote:

tump said:
spores form syrices to lc are fine on lc. Spore form print need agar.





lol, where do you think the spores in the syringes comes from? :rofl:


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InvisibleSupalemonhaze
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Re: Cyber LC Tek possible with smaller jar? [Re: wowimflabbergasted]
    #23461886 - 07/21/16 07:45 AM (7 years, 6 months ago)

Plenty of times, most of those were fails which is funny because I can keep an agar plate perfectly clean through multiple transfers. Haven't lost a grain jar in about 3 or 4 batches, my last one was from this really funky pan mycelium which I wasn't really sure about.

I've had relative success with LCs the first few times I used it. My first time was actually inoculated with a piece of clone, believe it or not and it gave me that PE minitub in my sig. Pretty sick. Still though, I would never inoculate an LC with spores. I have never even inoculated a grain jar with a syringe (unless purely for germination) but if I had no other way but to use a syringe, I would stick to spores>grains. Spores to LCs is just too risky.

You might get away with something funky in your syringe if you throw it in a grain jar but do that with an LC and that funky thing will grow and buttfuck you.


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Invisiblewowimflabbergasted
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Re: Cyber LC Tek possible with smaller jar? [Re: Supalemonhaze]
    #23461888 - 07/21/16 07:47 AM (7 years, 6 months ago)

All my first grows for a year were with LC from spores. I dunno man....it's not the devil like everyone here is making it out to be.



Edit: Maybe I just got lucky, who knows. :shrug:


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Edited by wowimflabbergasted (07/21/16 07:48 AM)


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InvisibleInocuole
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Re: Cyber LC Tek possible with smaller jar? [Re: wowimflabbergasted]
    #23461917 - 07/21/16 07:59 AM (7 years, 6 months ago)

Considering the kind of shit I see ALL the time when putting syringes to agar, I just can't in good conscience ever recommend putting them to LC.


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Re: Cyber LC Tek possible with smaller jar? [Re: Inocuole]
    #23461928 - 07/21/16 08:03 AM (7 years, 6 months ago)

im never talking about a fucking LC again  :rofl:


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Offlinetump
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Re: Cyber LC Tek possible with smaller jar? [Re: wowimflabbergasted]
    #23461955 - 07/21/16 08:17 AM (7 years, 6 months ago)

Dude agar is perfect spore form a spore print but when adding clean water and the spore print making your syrices different. Batrai runs better form spore syrice. To agar conpared to spore print to spores. Myc out runs in grain jars which is why it works some times. But diludes with more water in lc form. Makeing it clearing in the long run. The way you start out is every thing


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InvisibleSupalemonhaze
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Re: Cyber LC Tek possible with smaller jar? [Re: wowimflabbergasted]
    #23461965 - 07/21/16 08:22 AM (7 years, 6 months ago)

It's not that it's a guaranteed fail but nowadays there is enough info around to avoid most of the unneccessary fails. Agar is dirt cheap anyway. Those 2-3 plates it takes to ensure a clean culture will be cheaper than a whole batch of jars ruined when taking into account the supplies ruined, time wasted and energy used.

My point is that if you are hell bent on using syringes, you should do so on grains. A single dirty inoculation will give you a single dirty jar while if you use that on an LC, it will wreak havoc on your entire batch. One could say that he would test it on a test jar before but who's to say you will be able to spot it invitro? I've had plenty of molds go undetected up until fruiting, turning the whole thing green.

This isn't at all a statement saying "spores+LC=100% fail", it's a statement saying reduce your chances of contamination by as much as you can.


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Re: Cyber LC Tek possible with smaller jar? [Re: Supalemonhaze]
    #23461974 - 07/21/16 08:27 AM (7 years, 6 months ago)

Best chance would be to knock up i small grain jar like a half pint
Then remove two grain into like 50 plates. What is clean is clean to dp what you like or bad just tows away


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Offlinespacechildo
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Re: Cyber LC Tek possible with smaller jar? [Re: tump]
    #23461984 - 07/21/16 08:30 AM (7 years, 6 months ago)

Quote:

tump said:
Dude agar is perfect spore form a spore print but when adding clean water and the spore print making your syrices different. Batrai runs better form spore syrice. To agar conpared to spore print to spores. Myc out runs in grain jars which is why it works some times. But diludes with more water in lc form. Makeing it clearing in the long run. The way you start out is every thing




this makes absolutely no sense, both on a mycological and lingual level..
spore syringe to LC isnt better than spore print to LC, same dirty spores.
But I'm not even sure that's what you're saying anymore.. take a second a proof read your posts man!


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InvisibleSupalemonhaze
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Re: Cyber LC Tek possible with smaller jar? [Re: tump]
    #23461987 - 07/21/16 08:31 AM (7 years, 6 months ago)

So instead of doing spores>LC>grain>50plates, why not just do spores>agar>LC>agar?

If you have agar, this argument is already 6 feet under anyway. No one who uses agar is going to inoculate anything but an agar plate with spores.


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