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OfflineYeOlde
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Buy Spore Syringe only the once?
    #23452006 - 07/18/16 07:21 AM (7 years, 6 months ago)

Hi all,

If I buy a spore syringe how would I be able to keep myself in a perpetual "harvest"?

Would I ...

A) Inoculate some Agar medium
B) Once it's established then keep it in the fridge to halt growth of mycellium
C) Take slices from it, colonise a rye jar
D) colonise another jar with some of the colonised substrate from first jar
E) move some of the colonised substrate to a fresh jar and so on ?

Also how would you store the syringe?


--------------------
My Psychedelic experiences:
LSD - 30+ times (2 hits min / max 3 hits) every time.
Shrooms - 4 times (2.5 - 3.5g)
DMT - 5 times (Powerful breakthrough only once)

Life can be one hell of a bitter pill to swallow so I chose acid instead -YeOlde


Edited by YeOlde (07/18/16 07:27 AM)


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OfflineYerow
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Re: Buy Spore Syringe only the once? [Re: YeOlde]
    #23452017 - 07/18/16 07:28 AM (7 years, 6 months ago)

You can do that yup, agar to grain and then use those as masters for g2g and whatever :thumbup:


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OfflineYeOlde
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Re: Buy Spore Syringe only the once? [Re: Yerow]
    #23452047 - 07/18/16 07:37 AM (7 years, 6 months ago)

Ok cool, the PF Tek method can't do this though right, because the substrate "cakes" ?


--------------------
My Psychedelic experiences:
LSD - 30+ times (2 hits min / max 3 hits) every time.
Shrooms - 4 times (2.5 - 3.5g)
DMT - 5 times (Powerful breakthrough only once)

Life can be one hell of a bitter pill to swallow so I chose acid instead -YeOlde


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OfflineMorel Guy
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Re: Buy Spore Syringe only the once? [Re: YeOlde]
    #23452048 - 07/18/16 07:38 AM (7 years, 6 months ago)

Master spawn will run out of life sooner or later.  Professional labs such as Universities use preserving techniques to store cultures below freezing temps. 

Slants are the next best thing to keep a culture viable.


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OfflineYerow
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Re: Buy Spore Syringe only the once? [Re: Morel Guy]
    #23452060 - 07/18/16 07:43 AM (7 years, 6 months ago)

Yeah you can make liquid inoculant with blended colonized cakes but you really should go the agar way.

Slants/petridishes for storage of your cultures. Never used slants before but had petri dishes in my fridge working after roughly half a year in there, not been in the hobby long enough to store anything for longer time :smile:


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InvisibleSupalemonhaze
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Re: Buy Spore Syringe only the once? [Re: Yerow]
    #23452120 - 07/18/16 07:59 AM (7 years, 6 months ago)

Once you start using agar you can clean up your homemade prints as well so you don't have to rely on buying syringes that are "lab made" to "ensure" their cleanliness. You can put an MS inoculation into cold storage but this is a waste of space IMO. I would only slant an MS if I'm either giving my original print away or if it's PE, where you have to use swabs rather than prints. If you have a print, you can easily get a new culture by inoculating some agar. It can be as dirty as you like, you can still clean it with a little TLC.

Keep your test tubes for clones, isolates and for situations mentioned above. Of course, if equipment and fridge space isn't an issue, having a multispore slant will speed things up for whenever you decide to grow that certain variety. Making a transfer from a slant is faster than waiting for spores to germinate.

Just my :2cents:


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OfflineNDStepp84
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Re: Buy Spore Syringe only the once? [Re: Supalemonhaze]
    #23452480 - 07/18/16 10:41 AM (7 years, 6 months ago)

You can use agar to make a liquid inoculant to use to inoculate BRF cakes, but if you have a pressure cooker look into grains and bulk, less maintenance and higher yeild.


--------------------

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


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OfflineYeOlde
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Re: Buy Spore Syringe only the once? [Re: NDStepp84]
    #23452816 - 07/18/16 12:37 PM (7 years, 6 months ago)

Thanks so much for your replies. I am still undecided on which way to go for my first ever grow.

I was thinking about buying sterilised grow kits on ebay complete with substrates already done for me and just inoculate them.

If not, I will need to simply buy jars, rye grain, pressure cooker and some agar powder with petri dishes.

Is this about right?

Sorry for all the noob questions but apart from the PF Tek method on youtube that I see recommended here, is there an article on the shroomery that describes the "manual" way (way I just mentioned) with a clear guidance?

Or any guides you people recommend specifically with a high success rate and yield for a beginner?

There's a saying "Start as you mean to go on" - I'd rather do it the "proper" way from the beginning even if it's a little harder and learn properly but at the same time I do really hope to get a return.

EDIT: Just to clarify further, the guide I found recommended here is "Lets grow mushrooms" 4 part video on youtube. I do believe though that this way isn't the way that allows you to "bulk" grow

Thanks so much again for your advice!


--------------------
My Psychedelic experiences:
LSD - 30+ times (2 hits min / max 3 hits) every time.
Shrooms - 4 times (2.5 - 3.5g)
DMT - 5 times (Powerful breakthrough only once)

Life can be one hell of a bitter pill to swallow so I chose acid instead -YeOlde


Edited by YeOlde (07/18/16 12:44 PM)


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Offlinekingkc
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Re: Buy Spore Syringe only the once? [Re: YeOlde]
    #23452832 - 07/18/16 12:45 PM (7 years, 6 months ago)

I would pc some wheat berries and then drop some drops of spore solution in the jar. great for nwbies.


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Offlinespacechildo
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Re: Buy Spore Syringe only the once? [Re: YeOlde]
    #23452841 - 07/18/16 12:47 PM (7 years, 6 months ago)

dont but kits they arent sterile they all contam during storage and shipping.
lets grow mushrooms is for pf-tek not bulk, pro; dont need a PC, con; gives less yields,cant be g2g'd or shaken.


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OfflineYeOlde
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Re: Buy Spore Syringe only the once? [Re: spacechildo]
    #23452856 - 07/18/16 12:53 PM (7 years, 6 months ago)

Yep that's what I thought, PF TEK doesn't allow bulk grow so it's not really the answer.

I'm not sure what you mean by "PC" but regarding the kits, thank you. I'll avoid them.

Wheat berries in a jar (all sterilised) then inoculated is a recommended way to begin a bulk grow for a newbie? Do I just skip the agar in this case then and just shake the jars and spread the love to the next jars?

EDIT: Oops I got it, Pressure cooker!? yeah I see what you mean. That's ok I'll get one. I want to do it the best way. Yield is important to me.


--------------------
My Psychedelic experiences:
LSD - 30+ times (2 hits min / max 3 hits) every time.
Shrooms - 4 times (2.5 - 3.5g)
DMT - 5 times (Powerful breakthrough only once)

Life can be one hell of a bitter pill to swallow so I chose acid instead -YeOlde


Edited by YeOlde (07/18/16 01:00 PM)


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Offlinespacechildo
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Re: Buy Spore Syringe only the once? [Re: YeOlde]
    #23452868 - 07/18/16 01:00 PM (7 years, 6 months ago)

PC = pressure cooker.

I wouldn't skip agar, that's where all the fun stuff come from. contam's no fun.
you dont NEED petri dishes for agar you can use small containers, pastywhytes easy agar tek style.


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InvisibleTheChief
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Re: Buy Spore Syringe only the once? [Re: YeOlde]
    #23452965 - 07/18/16 01:37 PM (7 years, 6 months ago)

You can bulk with PF cakes, just cheese grate them into the bulk as your innoculant. Work the same as grain jars this way.

:chief:


--------------------


jcbowling1985 said:
"Additionally I did one Armageddon jar with all of the types together."


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OfflineBrozag
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Re: Buy Spore Syringe only the once? [Re: TheChief]
    #23453305 - 07/18/16 03:25 PM (7 years, 6 months ago)

im going g2g it easier and  faster  from spore syringe i need only do selection on agar and then infinitely g2g


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InvisibleTheChief
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Re: Buy Spore Syringe only the once? [Re: Brozag]
    #23453342 - 07/18/16 03:41 PM (7 years, 6 months ago)

:rockon:


--------------------


jcbowling1985 said:
"Additionally I did one Armageddon jar with all of the types together."


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